Recent Advances in Cardiac Repair and Regeneration

A special issue of Cells (ISSN 2073-4409).

Deadline for manuscript submissions: closed (15 October 2023) | Viewed by 2272

Special Issue Editor


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Guest Editor
Department of Cardiology, Institute CARDIOMET and CIC-Biotherapies, University Hospital of Toulouse, 31059 Toulouse, France
Interests: cardiology; heart failure; cardiac function

Special Issue Information

Dear Colleagues,

Despite the dramatic progression of available pharmacological therapies for the management of cardiovascular disease, the latter remains the major cause of death and disability worldwide. Damage irreversibility, myocardial remodeling process and inability to replace the dead cardiomyocytes constitute the main limitations of current invasive and non-invasive therapeutic approaches for cardiac diseases. Thus, restoring a normal myocardial function is a challenge for researchers. Reparative and regenerative medicine seems a promising therapy that may offer survival benefit for millions of people. It includes stem cells, extracellular vesicles, genetic modifications and bioactive factors with scaffolds made up of biodegradable and biocompatible materials. It is essential to consider both pathophysiological and immunomodulatory properties for evaluating their applicability in cardiac tissue repair and regeneration. This special issue provides a potential platform for publications on pathophysiology, pharmacological approach, biotherapies including stem cells and gene therapies in addition to biomaterial-based tissue (engineered cardiac patches) in the setting of cardiac repair and regeneration.

Prof. Dr. Jerome Roncalli
Guest Editor

Manuscript Submission Information

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Keywords

  • cardiac repair
  • cardiac regeneration
  • biotherapy
  • cell therapy
  • gene therapy

Published Papers (1 paper)

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Research

22 pages, 6453 KiB  
Article
Adult Multipotent Cardiac Progenitor-Derived Spheroids: A Reproducible Model of In Vitro Cardiomyocyte Commitment and Specification
by Mariangela Scalise, Fabiola Marino, Luca Salerno, Nunzia Amato, Claudia Quercia, Chiara Siracusa, Andrea Filardo, Antonio Chiefalo, Loredana Pagano, Giuseppe Misdea, Nadia Salerno, Antonella De Angelis, Konrad Urbanek, Giuseppe Viglietto, Daniele Torella and Eleonora Cianflone
Cells 2023, 12(13), 1793; https://doi.org/10.3390/cells12131793 - 5 Jul 2023
Cited by 1 | Viewed by 2020
Abstract
Background: Three-dimensional cell culture systems hold great promise for bridging the gap between in vitro cell-based model systems and small animal models to study tissue biology and disease. Among 3D cell culture systems, stem-cell-derived spheroids have attracted significant interest as a strategy to [...] Read more.
Background: Three-dimensional cell culture systems hold great promise for bridging the gap between in vitro cell-based model systems and small animal models to study tissue biology and disease. Among 3D cell culture systems, stem-cell-derived spheroids have attracted significant interest as a strategy to better mimic in vivo conditions. Cardiac stem cell/progenitor (CSC)-derived spheroids (CSs) provide a relevant platform for cardiac regeneration. Methods: We compared three different cell culture scaffold-free systems, (i) ultra-low attachment plates, (ii) hanging drops (both requiring a 2D/3D switch), and (iii) agarose micro-molds (entirely 3D), for CSC-derived CS formation and their cardiomyocyte commitment in vitro. Results: The switch from a 2D to a 3D culture microenvironment per se guides cell plasticity and myogenic differentiation within CS and is necessary for robust cardiomyocyte differentiation. On the contrary, 2D monolayer CSC cultures show a significant reduced cardiomyocyte differentiation potential compared to 3D CS culture. Forced aggregation into spheroids using hanging drop improves CS myogenic differentiation when compared to ultra-low attachment plates. Performing CS formation and myogenic differentiation exclusively in 3D culture using agarose micro-molds maximizes the cardiomyocyte yield. Conclusions: A 3D culture system instructs CS myogenic differentiation, thus representing a valid model that can be used to study adult cardiac regenerative biology. Full article
(This article belongs to the Special Issue Recent Advances in Cardiac Repair and Regeneration)
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