Decoding the Complexity of Angiogenesis: Insights into Vascular Formation and Disease

A special issue of Cells (ISSN 2073-4409). This special issue belongs to the section "Cells of the Cardiovascular System".

Deadline for manuscript submissions: 20 November 2025 | Viewed by 876

Special Issue Editor

Special Issue Information

Dear Colleagues,

Angiogenesis, in the strictest sense, is defined as the physiological process of vessel formation from the pre-existing vasculature. However, it is important to note that angiogenesis and vasculogenesis occur during embryonic development and in physiological and pathophysiological conditions. Angiogenesis is a fascinating fundamental biological process involving the interplay of different cell types and signaling molecules, and it is an extremely relevant therapeutic target under multiple disease conditions. The present Special Issue of Cells, entitled “Decoding the Complexity of Angiogenesis: Insights into Vascular Formation and Disease”, aims to publish high-quality original research and reviews dealing with this fascinating topic.

Key themes that will be explored within this Special Issue include mechanistic insights into cell–cell interactions, molecular downstream mechanisms, pathophysiologically relevant models, and mechanisms of developmental angiogenesis. Furthermore, translational approaches relevant to diseases with modified angiogenesis are highly appropriate.

Prof. Dr. Kay-Dietrich Wagner
Guest Editor

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Keywords

  • angiogenesis
  • vasculogenesis
  • development
  • physiological angiogenesis
  • pathophysiology
  • cell–cell communication
  • cell signaling

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Published Papers (1 paper)

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Research

17 pages, 6355 KB  
Article
Regulation of Hindbrain Vascular Development by rps20 in Zebrafish
by Xinyu Shen, Zhaozhi Wen, Shunze Deng, Yuxuan Qiu, Weijie Ma, Xinyue Dong, Jie Gong, Yu Zhang, Dong Liu and Bing Xu
Cells 2025, 14(14), 1070; https://doi.org/10.3390/cells14141070 - 13 Jul 2025
Viewed by 642
Abstract
During aging, the brain vasculature undergoes significant deterioration characterized by increased arterial tortuosity, compromised blood–brain barrier integrity, and reduced cerebral blood flow, all of which contribute to various neurological disorders. Thus, understanding the mechanisms underlying aging-related cerebrovascular defects is critical for developing strategies [...] Read more.
During aging, the brain vasculature undergoes significant deterioration characterized by increased arterial tortuosity, compromised blood–brain barrier integrity, and reduced cerebral blood flow, all of which contribute to various neurological disorders. Thus, understanding the mechanisms underlying aging-related cerebrovascular defects is critical for developing strategies to alleviate aging-associated neurological diseases. In this study, we investigated the role of aging-related genes in brain vascular development using zebrafish as an in vivo model. By thoroughly analyzing scRNA-seq datasets of mid- and old-aged brain vascular endothelial cells (human/mouse), we found ribosomal protein S20 (rps20) significantly down-regulated during aging. qPCR analysis and whole-mount in situ hybridization validated a high expression of rps20 during early zebrafish development, which progressively decreased in adult and aged zebrafish brains. Functional studies using the CRISPR/Cas9-mediated knockout of rps20 revealed an impaired growth of central arteries in the hindbrain and a marked increased intracranial hemorrhage incidence. Mechanistically, qPCR analysis demonstrated a significant downregulation of vegfa, cxcl12b, and cxcr4a, key signaling molecules required for hindbrain vascular development, in rps20-deficient embryos. In conclusion, our findings demonstrate that rps20 is essential for proper brain vascular development and the maintenance of vascular homeostasis in zebrafish, revealing a novel mechanism by which aging-related genes regulate brain vascular development. This study provides new insights that may aid in understanding and treating aging-associated vascular malformations and neurological pathologies. Full article
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