Electrochemical Biosensors: Advances and Prospects

Dear Colleagues,

Biosensors are now increasingly used in clinical diagnostics, the biomonitoring of clinical biomarkers, and targeted drug delivery. The need-based alliance of nanotechnology, biotechnology, and material science has revolutionized the field of biosensing for its diverse application. Of all the sensors, electrochemical biosensors are especially important as these are simple to fabricate, easy to operate, have portability, miniaturization ability, and facilitates rapid, sensitive, selective, on-site analysis in an economical manner. The future trend of electrochemical biosensors needs to focus on further improvisations in the amplification of various orders of magnitude, a lower detection limit, and the multiplex determination of target analytes in a high-throughput manner to realize the true potential.

This Special Issue will present comprehensive research outlining progress on the application of new materials or novel technology to improve the performance of electrochemical biosensors. Potential topics include electrochemical, electrochemiluminescent, and photoelectrochemical biosensors. We aim to attract both academic and industrial researchers in order to foster the current knowledge of electrochemistry and to present new ideas for biosensing applications. I cordially invite you to submit an article to this Special Issue. Both review articles and original research papers are welcomed.

Dr. Wenwen Tu
Guest Editor

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• biosensors
• electrochemistry
• electrochemiluminescence
• photoelectrochemistry
• immunoassay
• Cytosensor
• biomarkers
• nucleic acid assay
• pharmaceutical analysis
• drug delivery

Title: A simple and effective strategy for urease immobilization on PEDOT-based organic electrochemical transistors towards urea detection
Authors: Jael Neyra Recky; Juliana Scotto; Omar Azzaroni; Waldemar Marmisollé
Affiliation: Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA), Departamento de Química, Facultad de Ciencias Exactas, Universidad Nacional de La Plata (UNLP), CONICET. 64 and 113, La Plata 1900, Argentina
Abstract: We present the construction of an organic electrochemical transistor (OECT) based on poly(3,4-ethylendioxythiophene, PEDOT) and polyallylamine (PAH) and its evaluation as bioelectronic platform for urease integration and urea detection. The OECT channel was fabricated in a one-step procedure using chemical polymerization directly onto the interdigitated electrode. Through this method, a highly conductive organic film is obtained functionalized with amino groups that endow the channel surface with positive charge. Then, urease was immobilized on the surface by electrostatic interaction of the negatively charged enzyme at neutral pH with the positively charged surface yielding a highly stable architecture. The real-time monitoring of the urease adsorption process was achieved by registering the changes on the drain-source current of the OECT during the enzyme deposition with high sensitivity. Finally, the biosensing performance of the modified transistor was evaluated by measuring the changes in the conductivity of the OECT channel caused by the reaction products of the catalyzed hydrolysis of urea.