Journal Description
Crops
Crops
is an international, peer-reviewed, open access journal on the science and technology of crops published bimonthly online by MDPI.
- Open Access— free for readers, with article processing charges (APC) paid by authors or their institutions.
- High Visibility: indexed within ESCI (Web of Science), Scopus, and other databases.
- Journal Rank: JCR - Q2 (Agronomy) / CiteScore - Q2 (Agronomy and Crop Science)
- Rapid Publication: manuscripts are peer-reviewed and a first decision is provided to authors approximately 23.5 days after submission; acceptance to publication is undertaken in 6.8 days (median values for papers published in this journal in the first half of 2025).
- Recognition of Reviewers: APC discount vouchers, optional signed peer review, and reviewer names published annually in the journal.
- Crops is a companion journal of Agriculture and Agronomy.
Impact Factor:
1.9 (2024);
5-Year Impact Factor:
1.9 (2024)
Latest Articles
Predicting Plant Breeder Decisions Across Multiple Selection Stages in a Wheat Breeding Program
Crops 2025, 5(5), 69; https://doi.org/10.3390/crops5050069 - 2 Oct 2025
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Selection decisions in plant breeding programs are complex, and breeders aim to integrate phenotypic impressions, genotypic data, and agronomic performance across multiple selection stages to develop successful varieties. This study investigates whether such decisions can be predicted in a commercial winter wheat (
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Selection decisions in plant breeding programs are complex, and breeders aim to integrate phenotypic impressions, genotypic data, and agronomic performance across multiple selection stages to develop successful varieties. This study investigates whether such decisions can be predicted in a commercial winter wheat (Triticum aestivum L.) breeding program using elastic net models trained on genome-wide distributed markers and genomic estimated breeding values. For this purpose, a dataset of several thousand lines tested between 2015 and 2019 in preliminary, advanced, and elite multi-environment yield trials was analyzed across three decision-making scenarios. The predictive models achieved a higher precision than random selection in all scenarios, with an increased performance when genomic estimated breeding values were included as predictors. Comparisons of breeder selections and model recommendations in terms of selection differentials for key agronomic traits showed a substantial overlap in breeding objectives, while both the breeder’s decisions and the model’s suggestions maintained similar levels of genetic diversity. Although the precision of the elastic net model was of moderate magnitude, divergent model recommendations often identified promising alternative lines, highlighting the potential of artificial intelligence to support decision-making in plant breeding.
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Open AccessArticle
Impact of Coated Phosphorus Fertilizers and Application Methods on Soil Fertility, Yield, and Ionic Regulation of Common Beans (Phaseolus vulgaris L.) Grown in Saline Soil
by
Sara A. El-Shabasy, Tamer H. Khalifa, Tarek M. El-Zehery and Alaa El-Dein Omara
Crops 2025, 5(5), 68; https://doi.org/10.3390/crops5050068 - 29 Sep 2025
Abstract
Salinity is a major limitation on common bean productivity, while phosphorus in many soils is often immobilized, limiting its availability to plants. This study investigated the effects of coated and uncoated superphosphate fertilizers, applied at different rates and using distinct methods, on soil
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Salinity is a major limitation on common bean productivity, while phosphorus in many soils is often immobilized, limiting its availability to plants. This study investigated the effects of coated and uncoated superphosphate fertilizers, applied at different rates and using distinct methods, on soil properties, plant growth, and ion regulation in common beans grown in saline soil over two seasons (2023–2024). Treatments combined two fertilizer types (coated with potassium sulfate and uncoated), two P rates (360 and 480 kg/ha), and two application methods: (1) conventional application, broadcasting followed by plowing to 30 cm depth during soil preparation; (2) surface application, broadcasting without incorporation. Six treatments were applied: T1: 360 kg/ha of uncoated superphosphate (conventional method); T2: 480 kg/ha of uncoated superphosphate (conventional method); T3: 360 kg/ha of coated superphosphate (conventional method); T4: 480 kg/ha of coated superphosphate (conventional method); T5: 360 kg/ha of coated superphosphate (surface method); and T6: 480 kg/ha of coated superphosphate (surface method). The results demonstrated that soil pH was unaffected across treatments. However, T4 and T6 significantly improved nutrient availability (N, P, and K), biomass, grain yield, and seed nutritional quality (protein, P, K, and Ca). Despite increased soil EC, these treatments enhanced ionic balance (higher K/Na and Ca/Na ratios) indicating improved stress tolerance. Importantly, T3 (360 kg/ha coated) performed comparably to T2 (480 kg/ha uncoated), suggesting that coated superphosphate at lower rates can reduce input costs without compromising yield. These results demonstrate the agronomic and environmental benefits of coated superphosphate, particularly under saline conditions, through enhanced nutrient use efficiency and improved crop performance.
Full article
(This article belongs to the Topic Soil Fertility and Plant Nutrition for Sustainable Agriculture—2nd Edition)
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Open AccessArticle
Influence of Crop Phenology and Seasonality on Soil Conditions Across Depth Profiles
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Ramona Bălc, Delia Maria Gligor, Carmen Andreea Roba, Tiberius Dicu, Gheorghe Roșian and Laura Mico
Crops 2025, 5(5), 67; https://doi.org/10.3390/crops5050067 - 26 Sep 2025
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The regulation of nutrient availability and microbial processes in agroecosystems are strongly mediated by soil physico-chemical factors. Yet, their seasonal dynamics in different crops are not fully understood. This study monitored pH, redox potential (Eh), electrical conductivity (EC), and nitrite (NO2−
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The regulation of nutrient availability and microbial processes in agroecosystems are strongly mediated by soil physico-chemical factors. Yet, their seasonal dynamics in different crops are not fully understood. This study monitored pH, redox potential (Eh), electrical conductivity (EC), and nitrite (NO2−) in soils grown with clover, maize, and triticale from November to May. Monthly samples were collected in four depth layers (0–20, 20–40, 40–60, 60–80 cm) and analyzed to reveal patterns over time and space. Soil pH remained near neutral, with slight decreases in spring, and it appeared that maize maintained more stable values than clover or triticale. Eh was highest in winter, indicating oxidizing conditions, but decreased in spring, especially at depth under triticale. EC showed moderate variation, with higher surface values under maize. NO2− was uniformly low in winter but increased in spring, especially in deeper soils with triticale, while clover had lower accumulation. Overall, clover supported greater soil stability, maize increased surface EC, and triticale enhanced nitrite accumulation at depth. These results highlight the need for crop-specific, depth-aware management to maintain soil quality and optimize nitrogen cycling in agricultural systems.
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Open AccessCommunication
Evaluation of the Pathogenicity of Metarhizium taii and Trichoderma afroharzianum on Immature Stages of Bemisia tabaci in Tomato Plants
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Ricardo A. Varela-Pardo, Gustavo Curaqueo, Alejandra Fuentes-Quiroz, Paola Díaz-Navarrete, Claudia López-Lastra, Cecilia Mónaco and Eduardo Wright
Crops 2025, 5(5), 66; https://doi.org/10.3390/crops5050066 - 26 Sep 2025
Abstract
The whitefly (Bemisia tabaci) (Hemiptera: Aleyrodidae) is a small phytophagous invertebrate of herbaceous plants, shrubs, trees, wild plants, and crops of economic importance. It generates substantial economic losses due to direct damage caused by sap sucking and virus transmission. This work
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The whitefly (Bemisia tabaci) (Hemiptera: Aleyrodidae) is a small phytophagous invertebrate of herbaceous plants, shrubs, trees, wild plants, and crops of economic importance. It generates substantial economic losses due to direct damage caused by sap sucking and virus transmission. This work presents referential images of the morphology of B. tabaci and one of its main biological controllers in southern South America, thus serving as a reference for other researchers. In addition, results are presented of studies carried out to evaluate the pathogenicity of two fungal isolates (previously selected in vitro against Sclerotinia sclerotiorum and Botrytis cinerea and plant growth promoters) identified as Metarhizium taii CEP-722 and Trichoderma afroharzianum CEP-754 in immature stages of B. tabaci in tomato plants (Solanum lycopersicum). The trials were conducted under controlled conditions in controlled chambers, ensuring optimal growth conditions for B. tabaci, after morphological prospection, collection, identification, and mass rearing of adults in entomological cages. The results indicate that M. taii CEP-722 caused approximately 30% mortality in the immature stages of B. tabaci, while T. afroharzianum CEP-754 did not increase mortality under the experimental conditions. This study provides new knowledge on the potential of M. taii as a biological control agent against B. tabaci, offering a promising alternative in integrated pest management strategies. The results with T. afroharzianum suggest that further methodologies or combinations should be explored to improve its efficacy.
Full article
(This article belongs to the Topic Advances in Integrated Pest Management: New Tools and Tactics for Pest Control)
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Open AccessArticle
Water Deficit During Pod Development Affects Eco-Physiological Traits, Growth, and Yield in Pea Varieties Under Greenhouse Conditions in Tropical Highlands
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Diego Alejandro Gutiérrez-Villamil, Oscar Humberto Alvarado-Sanabria and Javier Giovanni Álvarez-Herrera
Crops 2025, 5(5), 65; https://doi.org/10.3390/crops5050065 - 25 Sep 2025
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Water deficit during reproductive development is one of the main constraints on pea (Pisum sativum L.) productivity in tropical highlands. In this study, five varieties with contrasting leaf architectures were evaluated under controlled greenhouse conditions, with and without water deficit applied from
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Water deficit during reproductive development is one of the main constraints on pea (Pisum sativum L.) productivity in tropical highlands. In this study, five varieties with contrasting leaf architectures were evaluated under controlled greenhouse conditions, with and without water deficit applied from the time of pod formation. Key ecophysiological variables, including leaf area index (LAI), radiation extinction coefficient (k), interception efficiency (RIE), radiation use efficiency (RUE), and water use efficiency (WUE), along with yield components, were measured. Deficit significantly reduced biomass, RUE, and yield, although the harvest index (HI) remained relatively stable. Varieties with the afila gene showed greater stability in LAI and WUE, but lower biomass accumulation. Correlation analyses revealed that, under optimal conditions, yield was closely associated with structural and functional traits, a relationship that weakened under stress. These results demonstrate the importance of integrating morphophysiological characteristics into breeding and agronomic management programs to develop more efficient and resilient varieties under water deficit conditions in the high tropics.
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Open AccessArticle
Seed Morphometry and Germination of Four Edible Species of Passiflora spp. Conserved in a Gene Bank
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Fabricio Verdezoto-Merino, Álvaro Monteros-Altamirano, Alberto Roura and Héctor Andrade-Bolaños
Crops 2025, 5(5), 64; https://doi.org/10.3390/crops5050064 - 23 Sep 2025
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The Passifloraceae family is one of the most representative in tropical America, with food, pharmaceutical, and ornamental importance. This study evaluated seed morphometry and germination of eight accessions of four Passiflora edible species, P. edulis; P. ligularis; P. quadrangularis; and
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The Passifloraceae family is one of the most representative in tropical America, with food, pharmaceutical, and ornamental importance. This study evaluated seed morphometry and germination of eight accessions of four Passiflora edible species, P. edulis; P. ligularis; P. quadrangularis; and P. tripartita var. mollissima, by studying accessions conserved several years in the gene bank (−15 °C) and recently collected accessions. Four experimental phases were carried out as follows: (1) morphometric characterization of seeds with qualitative and quantitative variables; (2) evaluation of germination under two thermal regimes (20 °C/30 °C and 25 °C); (3) application of six pre-germination treatments to overcome dormancy; and (4) tetrazolium tests. In phase 1, P. quadrangularis stood out for its unique morphological characteristics according to multivariate analysis. In phase 2, the alternating thermal regime (20 °C/30 °C) promoted the highest germination. In phase 3, the germination response was specific to each species: mechanical scarification in P. edulis (85.7%), KNO3 (0.5%) in P. ligularis (35.7%), control in P. quadrangularis (71.1%), and gibberellic acid (GA3 400 ppm) in P. tripartita (71.4%). The tetrazolium phase 4 identified the viability status of the seeds. It is concluded that the differences in morphometry and germination reflect the intrinsic characteristics of each species, highlighting the importance of specific protocols for their germination. This study provides tools to optimize the conservation and regeneration of Passiflora spp. germplasm under ex situ conditions, as a genetic base to be utilized in the future.
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Spring Wheat Breeding in Northern Kazakhstan: Drivers of Diversity and Performance
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Timur Savin, Yerlan Turuspekov, Akerke Amalova, Shynar Anuarbek, Adylkhan Babkenov, Vladimir Chudinov, Elena Fedorenko, Yelzhas Kairzhanov, Akerke Maulenbay, Grigoriy Sereda, Sergey Sereda, Daniyar Tajibayev, Vladimir Tsygankov, Artem Tsygankov, Lyudmila Zotova and Alexey Morgounov
Crops 2025, 5(5), 63; https://doi.org/10.3390/crops5050063 - 17 Sep 2025
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Kazakhstan cultivates over 12 million hectares of wheat, primarily spring wheat in the northern region. Spring wheat yields are low, ranging from 1.2 to 1.7 t/ha depending on weather conditions. Northern Kazakhstan is served by five spring wheat breeding programs: A.I. Barayev Research
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Kazakhstan cultivates over 12 million hectares of wheat, primarily spring wheat in the northern region. Spring wheat yields are low, ranging from 1.2 to 1.7 t/ha depending on weather conditions. Northern Kazakhstan is served by five spring wheat breeding programs: A.I. Barayev Research and Production Centre for Grain Farming and Agricultural Experimental Stations located in the Aktobe, Karagandy, Kostanay, and North Kazakhstan regions. In 2022, a germplasm set was assembled, including cultivars and breeding lines from the five breeding programs, totaling 84 genotypes. This set was evaluated in field trials during 2022 and 2023 at the breeding programs that contributed to the germplasm (except Aktobe). The material was also screened for molecular markers associated with genes for agronomic traits. The study objective was to compare the diversity and performance of germplasm originating from different breeding programs and identify potential underlying drivers. Breeding sites grouped based on variations in air temperature, precipitation, and grain yield demonstrated both similarities and differences among sites. However, these similarities were not reflected in the agronomic performance of materials originating from different locations. The expectation that germplasm would perform best for grain yield at its “home” location was not always confirmed. Grouping of germplasm based on genetic diversity of 20 molecular markers was not related to similarities in environmental conditions at the places of origin. The performance and diversity of germplasm from each of the five breeding programs is apparently driven by factors beyond environment, including breeding strategy and methodology, parental pool, and, in the absence of modern tools, breeders’ intuition and selection robustness. Kazakh spring wheat breeding programs require improvement to remain competitive in the face of increasing pressure from introduced foreign cultivars.
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Mitigating Salinity Stress in Solanaceae: The Role of Nanoparticles in Seed Germination and Growth Development
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Chinur Hadi Mahmood, Kamaran Salh Rasul and Hawar Sleman Halshoy
Crops 2025, 5(5), 62; https://doi.org/10.3390/crops5050062 - 12 Sep 2025
Abstract
Salinity is a significant challenge that limits agricultural productivity worldwide. This study examined the use of nanoparticles to improve the growth and development of Solanaceae crops under salinity stress. Specifically, titanium dioxide (TiO2NPs), copper oxide (CuONPs), and zinc oxide (ZnONPs) were
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Salinity is a significant challenge that limits agricultural productivity worldwide. This study examined the use of nanoparticles to improve the growth and development of Solanaceae crops under salinity stress. Specifically, titanium dioxide (TiO2NPs), copper oxide (CuONPs), and zinc oxide (ZnONPs) were applied at 750, 1250, and 1500 mg/kg per seed, respectively, to assess their effects on seed germination and growth of tomato, eggplant, and pepper plants. Results showed that tomato plants under salinity stress performed best with CuONPs, which improved key traits. The combination of salinity and TiO2NPs reduced flower abortion and increased seed yield and 1000-Seed weight. In eggplants, CuONPs and ZnONPs, both individually and in combination with salinity, enhanced plant characteristics, with CuONPs showing particularly strong effects. Control plants consistently recorded the lowest values across traits. For peppers, ZnONPs applied individually most effectively improved growth traits, while CuONPs reduced flower abortion and enhanced seed and germination rates. However, salinity stress itself severely reduced pepper growth parameters. The findings highlight the potential of nanoparticle applications to mitigate salinity stress, enhance growth performance, and support sustainable crop production in tomatoes, eggplants, and peppers, offering practical solutions for salinity-affected agriculture.
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(This article belongs to the Topic Plant Responses and Tolerance to Salinity Stress, 2nd Edition)
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Open AccessArticle
Moroccan Ulva rigida Extracts: A Promising Biostimulant for Improving Growth and Photosynthetic Performance in Salt-Stressed Bean Plants
by
Salma Latique
Crops 2025, 5(5), 61; https://doi.org/10.3390/crops5050061 - 8 Sep 2025
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Soil salinity is a crucial factor that limits agricultural production, negatively impacting the growth and physiological functions of salt-sensitive crops, such as beans. The present study examined the efficiency of Ulva rigida seaweed extracts (URE) as biostimulants to enhance the growth and photosynthetic
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Soil salinity is a crucial factor that limits agricultural production, negatively impacting the growth and physiological functions of salt-sensitive crops, such as beans. The present study examined the efficiency of Ulva rigida seaweed extracts (URE) as biostimulants to enhance the growth and photosynthetic ability of bean plants (Phaseolus vulgaris L.) under saline conditions (51.33 mM NaCl). Seaweed extracts were obtained by maceration and ultrasonic assistance at two concentrations, 25% and 50% (v/v), and applied as a foliar spray or irrigation. The most significant improvement was observed following foliar sprays of 50% ultrasonic extract (UP-50), with an increase of 96% in CCI compared to salt-stressed controls and by 71% compared to non-stressed controls. Stomatal conductance (SC) was also significantly improved with UP-50, reaching levels that were 146% higher than those of salt-stressed plants and 53% higher than those of non-stressed plants. The OJIP transients under salinity were significantly improved by both ultrasonic-assisted and maceration extracts; especially, 50% maceration extracts (MP-50) restored PSII quantum efficiency (ΦPo) and total performance index (PItotal) of salinity-stressed seedlings to 107% and 255% of non-stressed control and 122% and 314% of salt-stressed control, respectively. Root length and indole acetic acid (IAA) levels in treated plants were also enhanced, particularly in response to higher concentrations of the extract, suggesting improved root growth as well as hormonal homeostasis in the presence of salt stress. According to these findings, U. rigida extracts, specifically those applied at high concentrations as a foliar spray, serve as biostimulants that mitigate the adverse effects of salt stress on beans by preventing chlorophyll degradation and enhancing photosynthesis, root development, and hormonal balance.
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Open AccessArticle
Dual Role of Triazole Fungicides in Managing Alternaria Blight and Promoting Growth in Groundnut (Arachis hypogaea L.)
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Jyotika Purohit, Anirudha Chattopadhyay, Dasharathlal S. Patel, Somabhai M. Chaudhari and Kantilal K. Patel
Crops 2025, 5(5), 60; https://doi.org/10.3390/crops5050060 - 2 Sep 2025
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Alternaria leaf blight (ALB) is a major constraint to groundnut production, particularly in North Gujarat, where its incidence has intensified in recent years due to changing climatic conditions. Effective and sustainable disease management requires fungicides that not only suppress the pathogen but also
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Alternaria leaf blight (ALB) is a major constraint to groundnut production, particularly in North Gujarat, where its incidence has intensified in recent years due to changing climatic conditions. Effective and sustainable disease management requires fungicides that not only suppress the pathogen but also promote plant growth. To identify such options, field experiments were conducted during 2016–2018 to evaluate the bioefficacy of nine fungicides, including five systemic, two contact, and two combination formulations. Among these, propiconazole 25 EC, tebuconazole 25 WG, and carbendazim 50 WP were the most effective in reducing disease intensity and slowing disease progression. The highest pod and haulm yields were recorded in plots treated with tebuconazole 25 WG, followed by propiconazole 25 EC and carbendazim 50 WP. However, the highest cost–benefit ratio was observed with carbendazim 50 WP, followed by propiconazole 25 EC and tebuconazole 25 WG. In addition, propiconazole 25 EC and tebuconazole 25 WG exhibited notable plant growth-promoting effects, enhancing plant height, root length, and chlorophyll content. Based on these findings, the application of propiconazole 25 EC or tebuconazole 25 WG is recommended for the effective and economical management of ALB in groundnut.
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Open AccessArticle
Effect of Row Spacing in the Period Prior to Weed Interference in Peanut Cultivation Under Azorean Conditions
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Mariana Casari Parreira, Vasco Rafael Rodrigues Costa, David João Horta Lopes, João Martim de Portugal e Vasconcelos, João da Silva Madruga, Vitor Adriano Benedito, Arthur Nardi Campalle and Heytor Lemos Martins
Crops 2025, 5(5), 59; https://doi.org/10.3390/crops5050059 - 31 Aug 2025
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Peanut cultivation currently plays a minor role in Portuguese agriculture, despite the country’s favorable soil and climatic conditions. In the Azores archipelago, where agriculture is a key economic activity, peanut production has recently sparked interest among rural producers. Weeds pose a major threat
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Peanut cultivation currently plays a minor role in Portuguese agriculture, despite the country’s favorable soil and climatic conditions. In the Azores archipelago, where agriculture is a key economic activity, peanut production has recently sparked interest among rural producers. Weeds pose a major threat to crop development, particularly for short-cycle species like peanuts. This study aimed to determine the period prior to weed interference (PPI) in peanut crops under two row spacings (40 cm and 60 cm) on São Miguel Island, Azores. Eight treatments were established—0–15, 0–30, 0–45, 0–60, 0–75, 0–90 days after emergence (DAE), full-season coexistence, and a weed-free control—to represent increasing periods of weed competition. A randomized block design with four replicates was used for each spacing. The weed community included eight species, with Cyperus spp., Digitaria spp., Amaranthus blitum, and Portulaca oleracea being the most prevalent. Weed interference throughout the entire cycle led to yield losses exceeding 81% and 86% at 40 cm and 60 cm row spacings, respectively. The PPI was defined at a 5% yield reduction threshold, which is a commonly accepted benchmark in weed science to determine the beginning of the critical period of weed interference.
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Open AccessArticle
A Novel Spectral Vegetation Index for Improved Detection of Soybean Cyst Nematode (SCN) Infestation Using Hyperspectral Data
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Yuhua Wang, Ruopu Li, Jason Bond, Ahmad Fakhoury and Justin Schoof
Crops 2025, 5(5), 58; https://doi.org/10.3390/crops5050058 - 29 Aug 2025
Abstract
Soybean cyst nematode (SCN) is a pathogen with serious impacts on soybean yields, yet traditional field-based assessment is labor-intensive and often ineffective for early interventions, and the existing spectral vegetation indices (VIs) also lack the ability to accurately detect SCN infested plants. This
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Soybean cyst nematode (SCN) is a pathogen with serious impacts on soybean yields, yet traditional field-based assessment is labor-intensive and often ineffective for early interventions, and the existing spectral vegetation indices (VIs) also lack the ability to accurately detect SCN infested plants. This study aimed to develop an improved detection method using hyperspectral data. A greenhouse-based experiment was designed to collect 100 hyperspectral datasets from 20 soybean plants inoculated with four SCN egg levels (0–10,000) from the 68th to 97th day after planting. Based on spectral similarity and inoculation levels, three stress classes were defined as proxies for actual plant stress: healthy (0 egg), moderate (1000 and 5000 eggs), and severe (10,000 eggs). These classifications are based on predefined inoculation thresholds and spectral trends, which may not fully align with direct physiological stress measurements due to inherent variability in individual plant responses. Through analysis of variance (ANOVA), principal component analysis (PCA), feature selection, and classification comparison, a new spectral VI, called SCNVI, was proposed using bands 338 nm and 665 nm. The SCNVI coupled with eXtreme Gradient Boosting (XGBoost) achieved an accurate classification of 70% for three classes and outperformed the 12 traditional VIs. These findings suggest that integrating the SCNVI and XGBoost algorithm provides the potential for improving the detection of SCN infestation, though further validation in field environments is required to confirm its practical applicability.
Full article
(This article belongs to the Topic Advances in Integrated Pest Management: New Tools and Tactics for Pest Control)
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Open AccessArticle
Growth and Physiological Traits Associated with Water Use Efficiency in Different Popcorn Genotypes Grown Under Water-Stress Conditions
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Emile Caroline Silva Lopes, Paulo Ricardo dos Santos, Luciene Souza Ferreira, Guilherme Augusto Rodrigues de Souza, Weverton Pereira Rodrigues, Samuel Henrique Kamphorst, Valter Jário de Lima, Deivisson Pelegrino de Abreu, Antônio Teixeira do Amaral Junior and Eliemar Campostrini
Crops 2025, 5(5), 57; https://doi.org/10.3390/crops5050057 - 29 Aug 2025
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Climate change may soon impact popcorn productivity. The aim was to assess physiological and growth traits in two popcorn genotypes with different water use efficiency under water-deficit stress. The plants were grown in a greenhouse under either water stress (WS) or non-water stress
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Climate change may soon impact popcorn productivity. The aim was to assess physiological and growth traits in two popcorn genotypes with different water use efficiency under water-deficit stress. The plants were grown in a greenhouse under either water stress (WS) or non-water stress (WW) conditions. Gas exchange, chlorophyll fluorescence, and leaf temperature were assessed every three days, for a total of nine measurements. At the end of the assessment period, growth traits and the SPAD index were evaluated. Our hypotheses were as follows: (a) plants of the P7 genotype (water-efficient agronomic genotype) would take longer than L65 plants (water-inefficient agronomic genotype) to reduce photosynthetic rates under water stress conditions; (b) after re-irrigation, P7 plants would recover photosynthetic capacity with values similar to the period without water stress; and (c) P7 plants would recover photosynthetic capacity faster than L65 plants when subjected to the same period of water stress. The P7 genotype (agronomic water-efficient genotype) absorbed water more quickly due to higher root biomass, root length, and root volume. Yet, at 14 days after suspending irrigation (DASI), the P7 genotype had the lowest net CO2 assimilation rate (Anet), stomatal conductance (gs), and transpiration rates (E) values. However, L65 (agronomic water-inefficient genotype) had the lowest Anet, gs, and E values only at 17 DASI. As a consequence of stomatal closure in both genotypes, the E rates were reduced, and there was an increase in leaf temperature for WS plants, while L65 had higher leaf temperature at maximum water stress. No photochemical damage was detected, indicating that the reduced Anet in WS was likely due to stomatal limitations and biochemical disturbances in both genotypes. Photosynthetic recovery occurred gradually, with full restoration of rates in both genotypes at the end of the experiment. Although our initial hypothesis expected the P7 genotype to maintain photosynthesis longer under water stress, our findings showed an earlier decline in Anet compared to L65. This result is likely due to the large root system of P7 exhausting the limited soil water more rapidly in pot conditions, accelerating the onset of stress.
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Open AccessArticle
Valorization of Expired Milk into Protein Hydrolysate as a Plant Biostimulant: Characterization and Application on Hydroponically Grown Cos Lettuce
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Aryanis Mutia Zahra, Apiradee Uthairatanakij, Natta Laohakunjit, Pongphen Jitareerat, Nattapon Kaisangsri and Arak Tira-Umphon
Crops 2025, 5(5), 56; https://doi.org/10.3390/crops5050056 - 28 Aug 2025
Abstract
To meet global food demand, agricultural systems must enhance crop performance, productivity, and sustainability. Biostimulants have emerged as a promising strategy, particularly in vegetable production, due to their ability to enhance plant growth and resilience. This study characterized milk-derived protein hydrolysates (MPH) produced
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To meet global food demand, agricultural systems must enhance crop performance, productivity, and sustainability. Biostimulants have emerged as a promising strategy, particularly in vegetable production, due to their ability to enhance plant growth and resilience. This study characterized milk-derived protein hydrolysates (MPH) produced from expired milk and evaluated their potential as biostimulants for hydroponic cos lettuce. Hydrolysis of expired milk with hydrochloric acid achieved 94.55% hydrolysis and yielded 80.77% free amino acids. MPH was applied at volumes of 0, 1, 3, and 5 mL L−1 in combination with Hoagland and Arnon nutrient solution. The 1 mL L−1 (MPH1) treatment significantly increased shoot and root biomass and canopy size while reducing nitrate accumulation and enhancing total flavonoid and ascorbic acid content, as well as antioxidant capacity. HPLC analysis showed that MPH1 treatment promoted the accumulation of key metabolites, including vanillic acid, para-coumaric acid, salicylic acid, ferulic acid, gallic acid, syringic acid, quercetin, myricetin, and naringenin. MPH1 improved uptake of phosphorus, potassium, calcium, magnesium, and iron, contributing to mineral biofortification and nutritional quality. These results demonstrate that MPH at 1 mL L−1 is an effective biostimulant, improving yield and quality while reducing nitrate levels in hydroponically grown cos lettuce, offering a sustainable solution for food waste valorization.
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(This article belongs to the Topic Sustainable Food Production and High-Quality Food Supply)
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Open AccessArticle
Machine Learning vs. Langmuir: A Multioutput XGBoost Regressor Better Captures Soil Phosphorus Adsorption Dynamics
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Miltiadis Iatrou and Aristotelis Papadopoulos
Crops 2025, 5(4), 55; https://doi.org/10.3390/crops5040055 - 13 Aug 2025
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Accurate prediction of soil phosphorus (P) adsorption capacity is essential for efficient fertilizer management and environmental protection. Traditional isotherm models, such as the Langmuir equation, have been widely used to quantify P sorption, but they do not adequately capture the nonlinear and multivariate
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Accurate prediction of soil phosphorus (P) adsorption capacity is essential for efficient fertilizer management and environmental protection. Traditional isotherm models, such as the Langmuir equation, have been widely used to quantify P sorption, but they do not adequately capture the nonlinear and multivariate nature of soil systems. This study evaluates the performance of a multi-output XGBoost regression model trained on laboratory-measured P adsorption data from 147 soils, representing a wide range of textures, pH levels, and CaCO3 contents. The model was developed to simultaneously predict P adsorption at five different equilibrium concentrations (1, 2, 4, 6, and 10 mg/L). SHAP analysis and causal discovery via DirectLiNGAM revealed that initial Olsen P concentration and sand content are the primary factors reducing P adsorption. The multi-output XGBoost model was compared against classical Langmuir isotherms using an extended dataset of 10,389 soil samples. The extended dataset was binned into four groups based on Olsen P concentrations and four groups based on sand content. This binning was based on the identification of these variables as highly influential by the XGBoost model, and on their demonstrated causal relationship with soil P sorption capacity through causal inference analysis. The XGBoost model outperformed the Langmuir model in capturing the effect of Olsen P and sand content, as it predicted a 12.6% drop in P adsorption in the very high Olsen P group and a 19.2% drop in the very high sand content groups, which are substantially higher than the reductions estimated by Langmuir isotherms. These results demonstrate that machine learning models, trained on well-designed experimental data, offer a superior alternative to classical isotherms for modeling P sorption dynamics.
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Open AccessArticle
Trade-Off Between Yield and Water-Use Efficiency in Piper nigrum
by
Helane C. A. Santos, Joaquim A. L. Junior, Olavo P. Silva, Rafaela S. Guerino, Mariele C. Alves, Deiviane B. da Silva, William L. C. de Aviz, Maria do B. C. L. Medeiros, Oriel F. Lemos, João P. C. L. Both, Luana M. Luz and Lucas C. Costa
Crops 2025, 5(4), 54; https://doi.org/10.3390/crops5040054 - 12 Aug 2025
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Water-use efficiency (WUE) plays a crucial role in sustainable crop production, particularly in water-limited environments where maximizing natural resource use is essential. This study evaluated the physiological and agronomic performance of two Piper nigrum cultivars, Clonada and Uthirankotta, grown under different soil water
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Water-use efficiency (WUE) plays a crucial role in sustainable crop production, particularly in water-limited environments where maximizing natural resource use is essential. This study evaluated the physiological and agronomic performance of two Piper nigrum cultivars, Clonada and Uthirankotta, grown under different soil water potential conditions. The trial was conducted in a 1930 m2 field using a randomized block design and drip irrigation system, calibrated to 3.55 L h−1 with a uniformity of 97%. Soil water availability was managed based on daily tensiometer readings at 20 and 30 cm depths, triggering irrigation at defined tensions (10–55 kPa). Clonada exhibited higher net CO2 assimilation rates (A) and stomatal conductance (gs), but these responses did not lead to higher yields. In contrast, Uthirankotta consistently maintained superior water-use efficiency and yield across all soil moisture conditions by favoring water conservation and targeted biomass allocation over maximized gas exchange. Both cultivars performed optimally at a soil water potential range of 25–35 kPa, with declines in yield and gas exchange parameters at higher tensions (45–55 kPa). Under such conditions, Uthirankotta was 51.3% more water-use efficient and 40.8% more productive than Clonada. Based on this, a Principal Component Analysis (PCA) further demonstrated distinct physiological profiles, underscoring trade-offs between yield and water-use strategies. These results highlight the significance of cultivar selection for optimizing WUE and provide valuable insights into irrigation management and breeding programs aimed at boosting black pepper performance under water-limited conditions.
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Open AccessArticle
Physicochemical and Microbiological Properties of Hazelnuts from Three Varieties Cultivated in Portugal
by
Ana Cristina Ferrão, Raquel P. F. Guiné, Marco Silva, Arminda Lopes and Paula M. R. Correia
Crops 2025, 5(4), 53; https://doi.org/10.3390/crops5040053 - 7 Aug 2025
Abstract
Hazelnut is an important crop worldwide, and the characteristics of the fruits are quite variable according to a number of factors, including variety and cultivation conditions, which in turn can vary according to harvest year. This study aimed to investigate some physical and
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Hazelnut is an important crop worldwide, and the characteristics of the fruits are quite variable according to a number of factors, including variety and cultivation conditions, which in turn can vary according to harvest year. This study aimed to investigate some physical and chemical characteristics of three hazelnut varieties grown in Portugal (Grada de Viseu, Tonda di Giffoni and Butler) along two different harvesting years (2021 and 2022). Also, the microbial quality was investigated for its relevance to the conservation of the fruits. The physical properties evaluated were biometric characteristics and colour, the chemical components analysed were moisture, lipids, protein, ash and fibre, and the microbial properties investigated were the microorganisms, moulds and yeasts. The results showed that, generically, statistically significant differences were found between the three varieties under study on several properties investigated. The kernel was confirmed as the lighter part of all hazelnuts, and when comparing between varieties, Tonda di Giffoni presented the lighter fruits in both harvesting years. With respect to weight, the Tonda di Giffoni variety was the lightest in both harvest years. Moisture content was observed to be higher than the recommended limits for two of the samples (Grada de Viseu in 2021: 6.01 ± 0.26 g/100 g and Butler in 2022: 6.02 ± 0.37 g/100 g), although the difference was marginal given that the recommended limit is 6%. Not surprisingly, lipids were the major chemical component, ranging from 66.46 ± 1.67 to 70.14 ± 1.75 g/100 g in 2021 and from 64.38 ± 1.67 to 77.77 g/100 g in 2022. It was further observed that the three varieties presented a satisfactory microbiological quality. Finally, applying factor analysis with principal components and Varimax rotation, a solution that explains 92.8% of the variance was obtained. This study provided information that is relevant for the characterisation and evaluation of variability according to the year of hazelnuts of three varieties cultivated in Portugal.
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Open AccessReview
State of the Art on the Interaction of Entomopathogenic Nematodes and Plant Growth-Promoting Rhizobacteria to Innovate a Sustainable Plant Health Product
by
Islam Ahmed Abdelalim Darwish, Daniel P. Martins, David Ryan and Thomais Kakouli-Duarte
Crops 2025, 5(4), 52; https://doi.org/10.3390/crops5040052 - 6 Aug 2025
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Insect pests cause severe damage and yield losses to many agricultural crops globally. The use of chemical pesticides on agricultural crops is not recommended because of their toxic effects on the environment and consumers. In addition, pesticide toxicity reduces soil fertility, poisons ground
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Insect pests cause severe damage and yield losses to many agricultural crops globally. The use of chemical pesticides on agricultural crops is not recommended because of their toxic effects on the environment and consumers. In addition, pesticide toxicity reduces soil fertility, poisons ground waters, and is hazardous to soil biota. Therefore, applications of entomopathogenic nematodes (EPNs) and plant growth-promoting rhizobacteria (PGPR) are an alternative, eco-friendly solution to chemical pesticides and mineral-based fertilizers to enhance plant health and promote sustainable food security. This review focuses on the biological and ecological aspects of these organisms while also highlighting the practical application of molecular communication approaches in developing a novel plant health product. This insight will support this innovative approach that combines PGPR and EPNs for sustainable crop production. Several studies have reported positive interactions between nematodes and bacteria. Although the combined presence of both organisms has been shown to promote plant growth, the molecular interactions between them are still under investigation. Integrating molecular communication studies in the development of a new product could help in understanding their relationships and, in turn, support the combination of these organisms into a single plant health product.
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Open AccessArticle
Influence of Partial Vermicompost Tea Substitution for Mineral Nitrogen Fertilizers on Yield and Nutrient Content of Wheat Cultivars
by
Hashim Abdel-Lattif and Mohamed Abbas
Crops 2025, 5(4), 51; https://doi.org/10.3390/crops5040051 - 5 Aug 2025
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Chemical fertilizers pose significant risks to both human health and the environment. To investigate the effect of substituting nitrogen fertilizer with vermicompost tea on wheat yield, shoot chemical constituents, and grain quality under clay-loam soil conditions, two field experiments were conducted at the
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Chemical fertilizers pose significant risks to both human health and the environment. To investigate the effect of substituting nitrogen fertilizer with vermicompost tea on wheat yield, shoot chemical constituents, and grain quality under clay-loam soil conditions, two field experiments were conducted at the Faculty of Agriculture, Cairo University, Egypt, during the winter seasons of 2021–2022 and 2022–2023. A split-plot design in randomized complete blocks with three replications was employed. Vermicompost tea was assigned to the main plots, while wheat cultivars were assigned to the subplots. The cultivars were evaluated under four treatments involving partial substitution of mineral nitrogen (recommended dose of nitrogen (RDN%, 190 kg N ha−1): a control (90% of RDN + 25 kg vermicompost tea), 80% of RDN + 37.5 kg vermicompost tea, and 70% of RDN + 50 kg vermicompost tea. Nitrogen fertilizer (RDN%) was applied at rates of 190 (control), 170 (90%), 150 (80%), and 130 (70%) kg N ha−1. The results indicated that partially substituting mineral nitrogen with vermicompost tea significantly increased grain weight/Ha, chlorophyll A, chlorophyll B, carotenoids, nitrogen, phosphorus (P), and potassium (K) content in shoots, as well as ash, crude protein, crude fiber, total sugar, and N, P, and K content in wheat grains. The grain weight/Ha of the Sakha-95, Giza-171, and Sads-14 cultivars increased by 38.6%, 33.5%, and 39.3%, respectively, when treated with 70% RDN + 50 kg vermicompost tea. The combination of the Sads-14 cultivar and 70% RDN + 50 kg vermicompost tea resulted in the highest values for grain weight/ha (9.43 tons ha−1), chlorophyll A (1.39 mg/g), chlorophyll B (1.04 mg/g), N (5.08%), P (1.63%), and P (2.43%) content in shoots. The same combination also improved ash (2.89%), crude fiber (2.84%), and K (6.05%) content in grains. In conclusion, the application of vermicompost tea in conjunction with chemical fertilizers offers a viable alternative to using chemical fertilizers alone, promoting sustainable agricultural practices and improving wheat production. It is recommended that mineral nitrogen fertilizer be partially replaced with vermicompost tea to enhance both the productivity and grain quality of wheat while minimizing environmental pollution.
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Open AccessReview
Implications of Fertilisation on Soil Nematode Community Structure and Nematode-Mediated Nutrient Cycling
by
Lilian Salisi Atira and Thomais Kakouli-Duarte
Crops 2025, 5(4), 50; https://doi.org/10.3390/crops5040050 - 30 Jul 2025
Abstract
Soil nematodes are essential components of the soil food web and are widely recognised as key bioindicators of soil health because of their sensitivity to environmental factors and disturbance. In agriculture, many studies have documented the effects of fertilisation on nematode communities and
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Soil nematodes are essential components of the soil food web and are widely recognised as key bioindicators of soil health because of their sensitivity to environmental factors and disturbance. In agriculture, many studies have documented the effects of fertilisation on nematode communities and explored their role in nutrient cycling. Despite this, a key gap in knowledge still exists regarding how fertilisation-induced changes in nematode communities modify their role in nutrient cycling. We reviewed the literature on the mechanisms by which nematodes contribute to nutrient cycling and on how organic, inorganic, and recycling-derived fertilisers (RDFs) impact nematode communities. The literature revealed that the type of organic matter and its C:N ratio are key factors shaping nematode communities in organically fertilised soils. In contrast, soil acidification and ammonium suppression have a greater influence in inorganically fertilised soils. The key sources of variability across studies include differences in the amount of fertiliser applied, the duration of the fertiliser use, management practices, and context-specific factors, all of which led to differences in how nematode communities respond to both fertilisation regimes. The influence of RDFs on nematode communities is largely determined by the fertiliser’s origin and its chemical composition. While fertilisation-induced changes in nematode communities affect their role in nutrient cycling, oversimplifying experiments makes it difficult to understand nematodes’ functions in these processes. The challenges and knowledge gaps for further research to understand the effects of fertilisation on soil nematodes and their impact on nutrient cycling have been highlighted in this review to inform sustainable agricultural practices.
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(This article belongs to the Topic Soil Health and Nutrient Management for Crop Productivity)
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