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New Ways for Plant Genome Editing
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New Ways for Plant Genome Editing

A special issue of Genes (ISSN 2073-4425). This special issue belongs to the section "Plant Genetics and Genomics".

Deadline for manuscript submissions: closed (20 September 2021) | Viewed by 9000

Special Issue Editor

Dr. Götz Hensel

E-Mail Website
Guest Editor
Centre of Plant Genome Engineering (CPGE), Institute of Plant Biochemistry, Heinrich-Heine-University, 40225 Dusseldorf, Germany
Interests: genome editing; targeted mutagenesis using customized endonucleases; genetic transformation of cereal plants; phytohormones; molecular farming; plant-microbe-interactions
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

The development of customized endonucleases for biotechnological processing has taken basic molecular research to a new level. New enzymes or systems are being developed at unprecedented speed to enable the targeted modification of a given genome. The relatively simple use of RNA-guided CRISPR/Cas technology has especially inspired the scientific community. Applications range from medicine and agriculture to synthetic biology, to name but a few. Not only can new insights be gained for basic research, but also applications in curing genetic diseases or accelerating plant breeding. The importance of CRISPR/Cas technology was recently recognized by the award of the Nobel Prize in Chemistry.

Since the great dynamics of the technology make it difficult to maintain an overview, this Special Issue will summarize new approaches to genome editing. Original papers and review articles can be submitted, e.g., new enzymes or new systems of efficiently targeted genome modification, especially those that enable mechanisms of homology-directed repair or other ways of integrating synthetic RNA or DNA elements. The focus is not exclusively limited to CRISPR/Cas. All work in any organism that involves targeted modification of the genome will be evaluated.

Dr. Götz Hensel
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Genes is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • TALEN
  • CRISPR/Cas
  • Base editing
  • Prime editing
  • Targeted mutagenesis
  • Allele replacement
  • Precise breeding

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Published Papers (2 papers)

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17 pages, 1776 KiB  
Article
Improvement of Gene Delivery and Mutation Efficiency in the CRISPR-Cas9 Wheat (Triticum aestivum L.) Genomics System via Biolistics
by Jaclyn Tanaka, Bastian Minkenberg, Snigdha Poddar, Brian Staskawicz and Myeong-Je Cho
Genes 2022, 13(7), 1180; https://doi.org/10.3390/genes13071180 - 30 Jun 2022
Cited by 6 | Viewed by 2839
Abstract
Discovery of the CRISPR-Cas9 gene editing system revolutionized the field of plant genomics. Despite advantages in the ease of designing gRNA and the low cost of the CRISPR-Cas9 system, there are still hurdles to overcome in low mutation efficiencies, specifically in hexaploid wheat. [...] Read more.
Discovery of the CRISPR-Cas9 gene editing system revolutionized the field of plant genomics. Despite advantages in the ease of designing gRNA and the low cost of the CRISPR-Cas9 system, there are still hurdles to overcome in low mutation efficiencies, specifically in hexaploid wheat. In conjunction with gene delivery and transformation frequency, the mutation efficiency bottleneck has the potential to slow down advancements in genomic editing of wheat. In this study, nine bombardment parameter combinations using three gold particle sizes and three rupture disk pressures were tested to establish optimal stable transformation frequencies in wheat. Utilizing the best transformation protocol and a knockout cassette of the phytoene desaturase gene, we subjected transformed embryos to four temperature treatments and compared mutation efficiencies. The use of 0.6 μm gold particles for bombardment increased transformation frequencies across all delivery pressures. A heat treatment of 34 °C for 24 h resulted in the highest mutation efficiency with no or minimal reduction in transformation frequency. The 34 °C treatment produced two M0 mutant events with albino phenotypes, requiring biallelic mutations in all three genomes of hexaploid wheat. Utilizing optimal transformation and heat treatment parameters greatly increases mutation efficiency and can help advance research efforts in wheat genomics. Full article
(This article belongs to the Special Issue New Ways for Plant Genome Editing)
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Review

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18 pages, 1758 KiB  
Review
Advances in Gene Editing of Haploid Tissues in Crops
by Pankaj Bhowmik and Andriy Bilichak
Genes 2021, 12(9), 1410; https://doi.org/10.3390/genes12091410 - 13 Sep 2021
Cited by 8 | Viewed by 5141
Abstract
Emerging threats of climate change require the rapid development of improved varieties with a higher tolerance to abiotic and biotic factors. Despite the success of traditional agricultural practices, novel techniques for precise manipulation of the crop’s genome are needed. Doubled haploid (DH) methods [...] Read more.
Emerging threats of climate change require the rapid development of improved varieties with a higher tolerance to abiotic and biotic factors. Despite the success of traditional agricultural practices, novel techniques for precise manipulation of the crop’s genome are needed. Doubled haploid (DH) methods have been used for decades in major crops to fix desired alleles in elite backgrounds in a short time. DH plants are also widely used for mapping of the quantitative trait loci (QTLs), marker-assisted selection (MAS), genomic selection (GS), and hybrid production. Recent discoveries of genes responsible for haploid induction (HI) allowed engineering this trait through gene editing (GE) in non-inducer varieties of different crops. Direct editing of gametes or haploid embryos increases GE efficiency by generating null homozygous plants following chromosome doubling. Increased understanding of the underlying genetic mechanisms responsible for spontaneous chromosome doubling in haploid plants may allow transferring this trait to different elite varieties. Overall, further improvement in the efficiency of the DH technology combined with the optimized GE could accelerate breeding efforts of the major crops. Full article
(This article belongs to the Special Issue New Ways for Plant Genome Editing)
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