Genes and Their Regulatory Networks Underlying Secondary Metabolite Biosynthesis in Horticultural Crops

A special issue of Horticulturae (ISSN 2311-7524). This special issue belongs to the section "Genetics, Genomics, Breeding, and Biotechnology (G2B2)".

Deadline for manuscript submissions: 30 September 2026 | Viewed by 599

Special Issue Editors

National Wolfberry Engineering Research Center, Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan 751002, China
Interests: molecular breeding; DNA methylation; gene mining of quality traits; gene editing; transcriptional regulation of secondary metabolites

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Guest Editor
School of Life Sciences, Ningxia University, Yinchuan 750021, China
Interests: salt stress; plant physiology; protein modification; gene function

Special Issue Information

Dear Colleagues,

The fruits of horticultural plants are rich in secondary metabolites such as flavonoids, terpenoids, and alkaloids. Their content and composition are important quality indicators for evaluating fruit quality, and they also play a significant role in human health and response to adverse stress.

A large number of horticultural plants have completed whole-genome sequencing to identify the members of gene families that regulate important secondary metabolites, providing excellent gene resources for revealing the diversity of secondary metabolism formation and targeted breeding of varieties.

It is necessary to identify the members of the gene family that regulate the secondary metabolites of horticultural plants at the whole-genome level and obtain candidate genes through the correlation analysis of gene expression and secondary metabolites.

This Special Issue welcomes research on the identification and expression of related gene families in important secondary metabolites of horticultural plants, including the identification of gene family members, evolution and replication events, and studies related to the content of secondary metabolites and gene expression.

Dr. Yue Yin
Dr. Lingxia Wang
Guest Editors

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Keywords

  • horticultural crops
  • secondary metabolites
  • secondary metabolite biosynthesis pathway
  • biosynthesis mechanisms
  • transcription factors
  • regulatory networks
  • transcriptional regulation
  • epigenetic regulation
  • post-transcriptional regulation

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Published Papers (1 paper)

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Research

17 pages, 5350 KB  
Article
Dynamic Accumulation and Transcriptional Regulation of Alkylamides in Developing Zanthoxylum planispinum var. Dintanensis Fruits
by Hang Zhang, Ning Lv, Xinglin Wang, Huan Tian, Lunxian Liu, Tie Shen and Qingxiong Yang
Horticulturae 2026, 12(3), 386; https://doi.org/10.3390/horticulturae12030386 - 20 Mar 2026
Viewed by 372
Abstract
The accumulation dynamics and regulatory mechanisms of the alkylamides, the key pungent compounds in the fruits of Sichuan peppers, remain poorly understood. Using fruits of the Zanthoxylum planispinum var. dintanensis (Dintan) harvested at five key developmental stages, we comprehensively mapped the accumulation of [...] Read more.
The accumulation dynamics and regulatory mechanisms of the alkylamides, the key pungent compounds in the fruits of Sichuan peppers, remain poorly understood. Using fruits of the Zanthoxylum planispinum var. dintanensis (Dintan) harvested at five key developmental stages, we comprehensively mapped the accumulation of numbering compounds and their underlying molecular drivers by integrating HPLC-based metabolite profiling and de novo transcriptomics. Total alkylamide content increased during development, with hydroxyl-α-sanshool (HαSS) being predominant. The contributions of hydroxyl-β-sanshool (HβSS) and hydroxyl-ε-sanshool (HεSS) increased in later stages. Cluster and correlation analyses identified 51 candidate genes strongly correlated (|r| ≥ 0.6) with HαSS accumulation, predominantly enriched in fatty acid and branched-chain amino acid metabolism pathways. The expression patterns of five stearoyl-CoA desaturase (SCD) genes, one long-chain acyl-CoA synthetase (ACSL/fadD), and one S-(hydroxymethyl)glutathione dehydrogenase/alcohol dehydrogenase (frmA) gene closely mirrored HαSS accumulation. In contrast, 3-oxoacyl-[acyl-carrier-protein] synthase II (fabF) and one β-ketoacyl-CoA synthase (KCS) gene exhibited a negative correlation. Accordingly, a positive regulatory network was constructed for HαSS accumulation. These findings revealed key candidate targets for deciphering the molecular basis of its unique flavor and for breeding high-pungency cultivars. Full article
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