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Horticulturae
Special Issues
Advanced Strategies for Arthropod Pest Control in Horticultural Systems
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Advanced Strategies for Arthropod Pest Control in Horticultural Systems

A special issue of Horticulturae (ISSN 2311-7524). This special issue belongs to the section "Insect Pest Management".

Deadline for manuscript submissions: 20 September 2024 | Viewed by 2668

Special Issue Editors

Prof. Dr. Dejane Santos Alves

E-Mail Website
Guest Editor
Campus Santa Helena (UTFPR-SH), Universidade Tecnológica Federal do Paraná, Santa Helena 85892-000, PR, Brazil
Interests: botanical insecticides; selectivity and compatibility of botanical insecticides for non-target organisms; formulation of botanical insecticides
Prof. Dr. Geraldo Andrade Carvalho

E-Mail
Guest Editor
Department of Entomology, Universidade Federal de Lavras-UFLA, P.O. Box 3037, Lavras 37200-900, MG, Brazil
Interests: botanical insecticides; selectivity and compatibility of botanical insecticides and chemical pesticides for non-target organisms
Special Issues, Collections and Topics in MDPI journals
Dr. Elen de Lima Aguiar Menezes

E-Mail Website
Guest Editor
Laboratório de Controle Biológico de Insetos Fitófagos (LACOBIF), Departamento de Entomologia e Fitopatologia, Centro Integrado de Manejo de Pragas (CIMP), Área da Fitopatologia-UFRRJ, Rodovia BR 465, km 7, Seropédica, Rio de Janeiro 23897-000, Brazil
Interests: agricultural entomology; biological control; selectivity for non-target organisms; agroecology; organic agriculture

Special Issue Information

Dear Colleagues,

Climate change has substantially impacted horticulture, causing a reduction in production and sometimes making harvests unviable. Furthermore, arthropod pests cause damage to crops and increase production costs. The abusive and indiscriminate use of synthetic chemical insecticides leads to environmental contamination, selection of resistant arthropod populations, and toxic effects on non-target organisms. The damage caused by arthropods in the post-harvest period must also be considered. Given the above, defining advanced strategies for controlling arthropod pests is necessary.

The purpose of this Special Issue, entitled “Advanced Strategies for Arthropod Pest Control in Horticultural Systems”, is to contribute to disseminating new knowledge and results developed by researchers worldwide. Further contributions are expected regarding good agricultural practices (GAPs); use of bioinputs; semiochemicals; biological control; selectivity and/or compatibility of pesticides for non-target organisms; botanical insecticides; studies involving structure/activity and biochemical mode of action of pesticides; mode of action of new compounds; integrated pest management (IPM); and resistance of pests and other essential themes for advancing the state of the art. Research papers on products of uncertain chemical composition, for example, crude extracts and essential oils with incomplete chemical descriptions, are not accepted for review.

Prof. Dr. Dejane Santos Alves
Prof. Dr. Geraldo Andrade Carvalho
Dr. Elen de Lima Aguiar Menezes
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Horticulturae is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2200 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • integrated pest management
  • organic agriculture
  • good agricultural practices
  • arthropod pest control
  • biological control

Published Papers (1 paper)

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Research

13 pages, 3541 KiB  
Article
Identification of Two Diamondback Moth Parasitoids, Diadegma fenestrale and Diadegma semiclausum, Using LAMP for Application in Biological Control
by Hwayeun Nam, Min Kwon, Srinivasan Ramasamy and Juil Kim
Horticulturae 2022, 8(5), 366; https://doi.org/10.3390/horticulturae8050366 - 22 Apr 2022
Cited by 4 | Viewed by 2482
Abstract
The diamondback moth, Plutella xylostella L., is a lepidopteran pest that damages various vegetable plants belonging to the genus Brassica worldwide. Various biological controls, such as parasitoid wasps, have been used to control this pest. Among these, Diadegma semiclausum and Diadegmafenestrale are [...] Read more.
The diamondback moth, Plutella xylostella L., is a lepidopteran pest that damages various vegetable plants belonging to the genus Brassica worldwide. Various biological controls, such as parasitoid wasps, have been used to control this pest. Among these, Diadegma semiclausum and Diadegmafenestrale are widely used globally. In field-based biological control research, the investigation of the population dynamics of parasitoids and the rate of parasitism within the pest population is very important. However, achieving profundity in research is difficult when morphologically similar species coexist in the field. The morphological characteristics of D. semiclausum and D. fenestrale are very similar, and they both parasitize P. xylostella larvae. Therefore, to accurately identify these species, in this study, we developed a molecular diagnostic method by using loop-mediated isothermal amplification (LAMP). The mitochondrial genome of D. fenestrale and partial nucleotide sequences, including the ITS region of D. semiclausum, were analyzed for use as species diagnosis markers. The results showed that the homology of D. fenestrale to D. semiclausum was 94%, due to the excessively low homology of the D loop, but the actual homology was higher than 94%, particularly in the coding region. D. fenestrale species-specific primers for LAMP were designed based on the region encoding COX3, and the optimal diagnostic reaction condition for the four primers (F3, B3, FIP, and BIP) was 63 °C for 35 min. A species-specific primer capable of classifying D. semiclausum was developed based on the ITS2 region, and the optimal reaction condition for diagnosis was 63 °C for 40 min. Under optimal conditions for both species, upon addition of the loop primer LB, the reaction efficiency increased, and the reaction time was shortened by more than 5 min. The diagnostic limit concentration was up to 10 pg under both optimal conditions; therefore, it was possible to detect even very low concentrations. For both species, diagnosis was possible by using LAMP assay with a DNA-releasing technique, without a DNA extraction process, and by incubating a tissue sample or the homogenized whole body at 95 °C for 5 min. In the case of D. fenestrale, it was possible to diagnose the parasitoid in P. xylostella larvae. Therefore, the developed LAMP diagnostic method can be used in a variety of ways to determine whether P. xylostella has been parasitized in the process of field research and mass breeding, and to accurately distinguish the species that are parasitic to P. xylostella larvae. This LAMP-based diagnostic method can be applied to identify various parasitoids that are used for the biological control of P. xylostella. Full article
(This article belongs to the Special Issue Advanced Strategies for Arthropod Pest Control in Horticultural Systems)
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