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Horticulturae
Special Issues
In Vitro Propagation and Biotechnology of Horticultural Plants, 2nd Edition
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In Vitro Propagation and Biotechnology of Horticultural Plants, 2nd Edition

A special issue of Horticulturae (ISSN 2311-7524). This special issue belongs to the section "Propagation and Seeds".

Deadline for manuscript submissions: 30 December 2024 | Viewed by 1339

Special Issue Editors

Dr. Andrea Copetta

E-Mail Website
Guest Editor
CREA Research Centre for Vegetable and Ornamental Crops, Corso degli Inglesi 508, 18038 Sanremo, Imperia, Italy
Interests: in vitro propagation; doubled haploid; microspore embryogenesis; recovery
Special Issues, Collections and Topics in MDPI journals
Dr. Marco Savona

E-Mail Website
Guest Editor
CREA Research Centre for Vegetable and Ornamental Crops, Corso degli Inglesi 508, 18038 Sanremo, Imperia, Italy
Interests: morphogenesis; differentiation; in vitro culture; breeding
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

In vitro plant tissue cultures and the recent related biotechnologies are assuming increasing importance, especially in the recent severe climate change scenario, in the propagation of horticultural and ornamental species. Private agricultural companies are approaching the scientific world and research, recognising its importance, asking to solve specific problems. The production of plants can be supported by several in vitro approaches, such as the germination of recalcitrant seeds, embryo rescue, somatic embryogenesis and regeneration.

Furthermore, micropropagation can be successfully applied to maintain and multiply endemic or rare plants, or plants with unique and peculiar ornamental and/or horticultural characteristics. It is also possible to recover varieties that are disease- and pest-free. The identification of efficient regeneration protocols is required as the first fundamental step to be applied in genome editing approaches, with the scope, for example, to modulate stress resistance genes in vegetable, ornamental or aromatic crops. Histological investigations could also support the macroscopic responses and behaviour of the enhanced plants.

The aim of the Special Issue “In Vitro Propagation and Biotechnology of Horticultural Plants, 2nd Edition” is to report innovative research, tools, approaches and techniques in the applications of in vitro propagation techniques.

Dr. Andrea Copetta
Dr. Marco Savona
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Horticulturae is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2200 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • in vitro propagation
  • biotechnology
  • vegetables
  • aromatic and ornamental plants
  • growth regulator
  • tissue culture

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Published Papers (2 papers)

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Research

24 pages, 5850 KiB  
Article
Nutrient Deficiency-Induced Stress Improves Skincare Effects and Phytochemical Content of Green Extracts from Lamiaceae In Vitro Cultures
by Inês Mansinhos, Sandra Gonçalves, Raquel Rodríguez-Solana, Gema Pereira-Caro, José Manuel Moreno-Rojas and Anabela Romano
Horticulturae 2024, 10(9), 947; https://doi.org/10.3390/horticulturae10090947 - 5 Sep 2024
Abstract
The objective of this study was to investigate the impact of nutrient starvation on the growth, biochemical, metabolomic, and biological traits of Lavandula viridis L’Hér and Thymus lotocephalus G. López and R. Morales in vitro cultures. In both species, a reduction in shoot [...] Read more.
The objective of this study was to investigate the impact of nutrient starvation on the growth, biochemical, metabolomic, and biological traits of Lavandula viridis L’Hér and Thymus lotocephalus G. López and R. Morales in vitro cultures. In both species, a reduction in shoot growth and in the production of chlorophyll and carotenoids was observed in cultures grown under nutrient-deficient media (especially Fe and N) compared to those grown under control conditions. The highest levels of hydrogen peroxide and lipid peroxidation, two indicators of oxidative stress, were observed in L. viridis cultures grown under N deficiency and in T. lotocephalus under P and Fe limitation. The results demonstrated that nutrient deficiency led to a 72% and 62% increase in the quantified phenolic compounds in L. viridis and T. lotocephalus, respectively. The highest concentrations of the major compound in both species—rosmarinic acid—were observed in cultures grown under Mg-deficient (60.7 ± 1.0 mg/gDW) and Fe-deficient (50.0 ± 0.4 mg/gDW) conditions in L. viridis and T. lotocephalus, respectively. Furthermore, nutrient starvation enhanced the capacity of green extracts to inhibit three enzymes (tyrosinase, elastase, and hyaluronidase) associated with anti-aging and their antioxidant properties. Full article
(This article belongs to the Special Issue In Vitro Propagation and Biotechnology of Horticultural Plants, 2nd Edition)
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22 pages, 3688 KiB  
Article
In Vitro Germination, Micropropagation and Addressing the Hyperhydricity of the Balkan Native Dianthus cruentus, a Plant with High Ornamental and Xeriscaping Potential
by Apostolos-Emmanouil Bazanis and Maria Papafotiou
Horticulturae 2024, 10(8), 813; https://doi.org/10.3390/horticulturae10080813 - 31 Jul 2024
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Abstract
Dianthus cruentus Griseb. (Caryophyllaceae) is an herbaceous perennial native to Greece with a strong ornamental potential when used as a pollinator-friendly component of xeric gardens and green roofs, where it is valued for its tolerance of poor, dry soils, and its showy colorful [...] Read more.
Dianthus cruentus Griseb. (Caryophyllaceae) is an herbaceous perennial native to Greece with a strong ornamental potential when used as a pollinator-friendly component of xeric gardens and green roofs, where it is valued for its tolerance of poor, dry soils, and its showy colorful inflorescences. Aiming to develop an efficient mass propagation protocol appropriate for the introduction of the species as a novel floricultural crop, the in vitro seed and clonal propagation of a Greek native xeric ecotype were investigated in this paper for the first time. A total of 90–100% of the seeds, after being stored in the dark at room temperature for 12 months, germinated when incubated at 10 to 25 °C after their surface sterilization and transfer in vitro. Sixty-day-old seedlings grown in vitro were then used as a source of nodal explants for the initial establishment of micropropagation cultures, more efficiently on MS medium with 0.1 mg L−1 6-benzylaminopurine (BA). In the multiplication stage, either normal or hyperhydric micro-shoots were used as explant sources, assessing the possibility of incorporating usually discarded material in the propagation procedure. Different solid media were tested, with the highest multiplication indices (5.1) recorded in an MS medium containing 0.1 mg L−1 BA and 0.05 mg L−1 NAA, regardless of explants’ hyperhydricity, while an MS medium containing 0.1 mg L−1 BA and 12 g L−1 agar proved optimal for the effective reversal of hyperhydric explants (MI: 5.2). Despite higher hyperhydricity and reaction rates being observed when hyperhydric explants were used, modifications in the multiplication medium proved to be highly effective in controlling hyperhydricity, with the highest number of normal shoots (2.4–2.6) produced in BA-containing media. Micro-shoots rooted readily in ½ MS medium (60–100%), with rooting rates and quality positively affected by the presence of 0.5 mg L−1 IBA in the rooting medium and the absence of cytokinins in the multiplication one. Rooted micro-shoots were successfully acclimatized ex vitro at high rates (65–100%), their origin influencing their acclimatization and morphology. Thus, the concurrent use of normal and hyperhydric shoots in the proposed micropropagation protocol is proven to be both feasible and desirable, as it is able to significantly increase efficiency and facilitate the sustainable exploitation and dissemination of D. cruentus as a promising multivalent horticultural crop. Full article
(This article belongs to the Special Issue In Vitro Propagation and Biotechnology of Horticultural Plants, 2nd Edition)
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