- Article
Tapirira obtusa Bark as a Natural Agent for Inflammation Reduction and Infection Control in Orotracheal Tubes
- Soraia Salman,
- Josy Goldoni Lazarini and
- Daniel Saraiva Lopes
- + 7 authors
Background: Tracheostomy procedures are associated with increased risk of nosocomial infections due to microbial colonization and biofilm formation on tube surfaces. These biofilms contribute to persistent infections and hinder clinical recovery. Plant-derived products have gained interest as alternative strategies for preventing device-associated infections. Methods: This study evaluated the phytochemical composition and the antimicrobial, anti-adherent, antibiofilm, anti-inflammatory, antioxidant, and toxicity properties of Tapirira obtusa bark extract (TOBE). Antimicrobial activity was determined by minimum inhibitory concentrations (MICs). Biofilm formation and microbial viability were assessed in mono- and mixed-species biofilms. Anti-inflammatory effects were evaluated by NF-κB inhibition and TNF-α quantification. Antioxidant activity was measured using the DPPH assay. Phytochemical analysis identified major bioactive groups, and toxicity was tested in the Galleria mellonella model. Results: TOBE exhibited notable antimicrobial activity, with MIC values between 3.9 and 31.25 µg/mL. At 78 µg/mL, the extract significantly reduced biofilm biomass and microbial viability (p < 0.05). TOBE also downregulated NF-κB activation and decreased TNF-α levels. Antioxidant assays confirmed radical-scavenging capacity. Phytochemical screening revealed phenolics, flavonoids, and tannins, and toxicity results indicated a safe profile. Conclusion: TOBE effectively inhibits microbial growth and biofilm development on orotracheal tube surfaces while exhibiting anti-inflammatory and antioxidant properties without detectable toxicity. These findings support its potential as a plant-based adjunct for preventing tracheostomy-related infections and improving patient outcomes.
5 January 2026


