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Edible Mushrooms: Advances and Perspectives
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Edible Mushrooms: Advances and Perspectives

A special issue of Journal of Fungi (ISSN 2309-608X). This special issue belongs to the section "Fungal Evolution, Biodiversity and Systematics".

Deadline for manuscript submissions: 30 November 2026 | Viewed by 1031

Special Issue Editors

Prof. Dr. Aniello Crescenzi

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Guest Editor
Department of Agricultural, Forestry, Food and Environmental Sciences (DAFE), University of Basilicata, Viale dell’Ateneo Lucano 10, 85100 Potenza, Italy
Interests: edible mushrooms; fungal biology; sustainable production systems; disease management in cultivated mushrooms
Prof. Dr. Ippolito Camele

E-Mail Website
Guest Editor
Department of Agricultural, Forestry, Food and Environmental Sciences (DAFE), University of Basilicata, Viale dell’Ateneo Lucano 10, 85100 Potenza, Italy
Interests: plant disease; natural products; molecular diagnosis; bioactive substances; microbiology; biological control
Special Issues, Collections and Topics in MDPI journals
Dr. Hazem Salaheldin Elshafie

E-Mail Website
Guest Editor
Department of Agricultural, Forestry, Food and Environmental Sciences (DAFE), University of Basilicata, Viale dell’Ateneo Lucano 10, 85100 Potenza, Italy
Interests: natural products; microbiology; plant pathology; antimicrobial activities; bioactive secondary metabolites
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Edible mushrooms represent a biological group of increasing scientific, nutritional, and economic relevance due to their high taxonomic diversity, favorable nutritional profile, and substantial biotechnological potential. Long appreciated as food resources, they have recently gained renewed attention because of their richness in bioactive metabolites with potential health-promoting properties and their contribution to the development of sustainable, low-input food production systems.

A thorough understanding of the physiological, molecular, and ecological processes underlying the growth, development, and fructification of edible mushrooms, as well as their interactions with cultivation substrates and environmental factors, is essential to improve production efficiency and product quality. In this regard, the integration of omics-based approaches, advanced biotechnological tools, and circular economy strategies is fostering innovation and sustainability across mushroom production chains.

This Special Issue invites original research articles and comprehensive review papers addressing edible mushrooms, including, but not limited to, studies on biodiversity, genetics and genomics, cultivation technologies, nutritional quality, food safety, and the valorization of by-products. Contributions that adopt integrated, multidisciplinary, and sustainable approaches along the entire production chain, from cultivation to consumption, are particularly encouraged, with the aim of providing a timely overview of recent advances and future perspectives in this dynamic research field.

Prof. Dr. Aniello Crescenzi
Prof. Dr. Ippolito Camele
Dr. Hazem Salaheldin Elshafie
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 250 words) can be sent to the Editorial Office for assessment.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Journal of Fungi is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • edible mushrooms
  • bioactive compounds
  • omics approaches
  • cultivation technologies
  • biotechnological applications

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Published Papers (2 papers)

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Research

18 pages, 1915 KB  
Article
Comparative Evaluation of YOLOv8 and YOLOv11 for Digital Phenotyping of Edible Mushrooms Under Controlled Cultivation Conditions
by Doo-Ho Choi, Youn-Lee Oh, Minji Oh, Eun-Ji Lee, Sung-I Woo, Minseek Kim and Ji-Hoon Im
J. Fungi 2026, 12(4), 232; https://doi.org/10.3390/jof12040232 - 24 Mar 2026
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Abstract
Digital phenotyping is increasingly recognized as an essential tool for the quantitative analysis of fungal morphology, particularly in controlled indoor cultivation systems where large numbers of fruiting bodies must be assessed consistently and non-destructively. While YOLOv8-based deep learning approaches have previously been applied [...] Read more.
Digital phenotyping is increasingly recognized as an essential tool for the quantitative analysis of fungal morphology, particularly in controlled indoor cultivation systems where large numbers of fruiting bodies must be assessed consistently and non-destructively. While YOLOv8-based deep learning approaches have previously been applied in phenotypic analyses of edible mushrooms, the applicability of newer YOLO architectures to fungal phenotyping remains largely unexplored. In this study, we present a controlled-environment digital phenotyping framework for indoor mushroom cultivation and conduct a systematic benchmarking evaluation of YOLOv11 for phenotypic segmentation in comparison with YOLOv8. Using bottle-cultivated Pleurotus ostreatus and Flammulina velutipes as representative edible basidiomycetes, we performed a controlled comparison of YOLOv8-seg and YOLOv11-seg using identical datasets, preprocessing pipelines, and hyperparameter configurations. The results demonstrate that YOLOv11 achieves segmentation performance comparable to that of YOLOv8 across all evaluated metrics (ΔmAP50–95 < 0.01) while substantially reducing computational complexity, including fewer trainable parameters, lower FLOPs, and decreased gradient load. Validation against caliper-based physical measurements revealed moderate, trait-dependent agreement, whereas inter-model consistency between YOLOv8 and YOLOv11 remained consistently high across diverse morphological and segmentation scenarios. These findings suggest that recent developments in object detection architectures can improve computational efficiency without compromising phenotypic measurement fidelity. More broadly, this study highlights the importance of periodically evaluating emerging detection architectures within biological phenotyping pipelines to ensure scalable, sustainable, and high-throughput fungal phenotyping under controlled-environment cultivation systems. Full article
(This article belongs to the Special Issue Edible Mushrooms: Advances and Perspectives)
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23 pages, 1464 KB  
Article
Comparative Optimization of Microwave and Ultrasound-Assisted Extraction of Bioactive Compounds and Proteins from Craterellus cornucopioides
by Mojca Čakić Semenčić, Filip Šupljika, Anet Režek Jambrak, Monika Kovačević, Nina Čuljak, Ivana Repić, Ksenija Markov and Jadranka Frece
J. Fungi 2026, 12(3), 215; https://doi.org/10.3390/jof12030215 - 17 Mar 2026
Viewed by 506
Abstract
This study investigates the optimization of extraction processes for bioactive compounds and proteins from the mushroom Craterellus cornucopioides by comparing Microwave-Assisted Extraction and Ultrasound-Assisted Extraction. Using Response Surface Methodology, the effects of temperature or amplitude, time, and solvent type were evaluated on total [...] Read more.
This study investigates the optimization of extraction processes for bioactive compounds and proteins from the mushroom Craterellus cornucopioides by comparing Microwave-Assisted Extraction and Ultrasound-Assisted Extraction. Using Response Surface Methodology, the effects of temperature or amplitude, time, and solvent type were evaluated on total phenols, flavonoids, proteins, glutathione content, and antioxidant capacity measured by DPPH and FRAP assays. Additionally, the antimicrobial potential of the extracts was screened against various pathogens. Results demonstrated that water was the most effective solvent for nearly all parameters across both techniques, providing a unified optimum in the ultrasound system at six minutes and one hundred percent amplitude. However, a notable exception was observed for glutathione recovery in the microwave system, where ethanol proved superior to water. Ultrasound-assisted extraction consistently outperformed microwave extraction in protein yield and overall antioxidant potential, offering a more robust approach regarding process efficiency and bioactive yield. In conclusion, while both green techniques enhance recovery, ultrasound extraction with water establishes itself as the most consistent method for the simultaneous extraction of bioactives. Full article
(This article belongs to the Special Issue Edible Mushrooms: Advances and Perspectives)
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