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Marine Antimicrobial Peptides and Antibiotic Resistance: From Oceanic Defense Systems to Translational Applications

A special issue of Marine Drugs (ISSN 1660-3397). This special issue belongs to the section "Marine-Derived Ingredients for Drugs, Cosmeceuticals and Nutraceuticals".

Deadline for manuscript submissions: 30 June 2026 | Viewed by 2299

Special Issue Editor


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Guest Editor
College of Marine Life Sciences, Ocean University of China, 5 Yushan Road, Qingdao, China
Interests: antibiotic resistance genes; antibiotic pollution control; antimicrobial peptide design; peptide functional characterization; genetic engineering

Special Issue Information

Dear Colleagues,

Marine antimicrobial peptides (AMPs) represent a crucial component of innate immunity across diverse marine taxa, ranging from micro- to multicellular organisms. These molecules exhibit potent activity against a wide array of pathogens and have garnered attention as promising alternatives to traditional antibiotics. This Special Issue aims to comprehensively capture the current progress and future directions in the discovery, characterization, and application of marine-derived AMPs and antimicrobial agents.

We welcome submissions covering the full spectrum of marine AMP research, including but not limited to novel AMP discovery and design; AMP databases and computational platforms; AMP–microbiome interactions in marine environments; advanced techniques for extraction, purification, and structural analysis; antibiotic resistance in marine bacteria; the interplay between AMPs and resistance genes; resistome dynamics; AMP-based alternatives to antibiotics; and strategies for antimicrobial resistance (AMR) control. Studies leveraging artificial intelligence (AI), synthetic biology, and systems biology to accelerate AMP development are particularly encouraged. Contributions addressing the implications for human health, aquaculture sustainability, and environmental microbiology are also highly welcome.

This Special Issue will serve as a broad and integrative platform for interdisciplinary research connecting marine biology, microbiology, drug discovery, and precision health, advancing both the fundamental understanding and translational applications of marine antimicrobial agents.

Dr. Pengfei Cui
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 250 words) can be sent to the Editorial Office for assessment.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Marine Drugs is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2900 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • antimicrobial peptides
  • marine peptides
  • bioactive compounds
  • antibiotic resistance
  • marine microbiology
  • resistome
  • peptidomics
  • host–pathogen interaction
  • drug discovery
  • AI-driven peptide discovery
  • synthetic biology
  • microbial ecology
  • peptide engineering
  • marine genomics
  • therapeutic peptides
  • natural product biosynthesis

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Published Papers (2 papers)

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Research

24 pages, 5640 KB  
Article
Recombinant Expression and Antimicrobial Mechanism of Cysteine-Rich Antimicrobial Peptides from Tigriopus japonicus Genome
by Dan Pu, Hongwei Tao, Jingwei Pang, Huishao Shi, Junjian Wang and Wei Zhang
Mar. Drugs 2026, 24(1), 45; https://doi.org/10.3390/md24010045 - 16 Jan 2026
Viewed by 1020
Abstract
The misuse of antibacterial agents has contributed to the growing prevalence of antibiotic resistance, highlighting an urgent need to explore alternative anti-infection therapeutic strategies. Antimicrobial peptides (AMPs) are naturally occurring molecules. They exhibit broad-spectrum antimicrobial activity and represent promising candidates for the development [...] Read more.
The misuse of antibacterial agents has contributed to the growing prevalence of antibiotic resistance, highlighting an urgent need to explore alternative anti-infection therapeutic strategies. Antimicrobial peptides (AMPs) are naturally occurring molecules. They exhibit broad-spectrum antimicrobial activity and represent promising candidates for the development of novel therapeutics. A cysteine-rich antimicrobial peptide was identified and characterized from the genome of Tigriopus japonicus and designated “TjRcys1”. The precursor form of TjRcys1 comprises 96 amino acids. Structural analyses of TjRcys1 revealed random coils, two α-helices, and two β-strands. Recombinant TjRcys1 had inhibitory effects upon Staphylococcus aureus and Bacillus sp. T2, with a minimum inhibitory concentration of 64 μM for both. TjRcys1 did not show complete inhibition against Vibrio alginolyticus, Klebsiella pneumoniae, or Aeromonas hydrophila at 64 μM, but it did slow their growth rate. TjRcys1 could disrupt the permeability of the cell membrane of S. aureus. Transcriptomic analyses indicated that TjRcys1 could interfere with the ribosome biosynthesis and nucleotide metabolism of K. pneumoniae. Our results provide a valuable reference for the development of new AMPs and optimization of their design. Full article
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16 pages, 1035 KB  
Article
Proteomic and Functional Characterization of Antimicrobial Peptides Derived from Fisheries Bycatch via Enzymatic Hydrolysis
by Vicky Balesteros S. Blumen Galendi, Guilherme Rabelo Coelho, Letícia Murback, Wagner C. Valenti, Tavani Rocha Camargo, Marcia Regina Franzolin, Daniel Carvalho Pimenta and Rui Seabra Ferreira, Jr.
Mar. Drugs 2026, 24(1), 36; https://doi.org/10.3390/md24010036 - 10 Jan 2026
Viewed by 881
Abstract
Fisheries bycatch, while representing a major ecological concern due to the incidental capture of non-target species, also constitutes an underexplored source of marine biomass with biotechnological potential. This study aimed to generate and characterize bioactive peptides from the muscle tissue of three common [...] Read more.
Fisheries bycatch, while representing a major ecological concern due to the incidental capture of non-target species, also constitutes an underexplored source of marine biomass with biotechnological potential. This study aimed to generate and characterize bioactive peptides from the muscle tissue of three common bycatch species from the Brazilian coast: Paralonchurus brasiliensis, Micropogonias furnieri, and Hepatus pudibundus. Muscle homogenates were hydrolyzed using either Alcalase or Protamex to produce peptide-rich hydrolysates, which were analyzed through SDS-PAGE, HPLC-UV, MALDI-TOF, and LC-MS/MS. De novo sequencing and bioinformatic analyses predicted bioactivities that were subsequently validated by in vitro assays. The results demonstrated that enzyme selection strongly influenced both peptide profiles and bioactivity. The Protamex hydrolysate of P. brasiliensis (PBP) exhibited potent antifungal activity, inhibiting Candida albicans growth by 81%, whereas the Alcalase hydrolysate (PBA) showed moderate inhibition of Staphylococcus aureus (29%). No significant effect was observed against Escherichia coli. Overall, this study highlights a sustainable strategy for the valorization of fisheries bycatch through the production of bioactive marine peptides and identifies P. brasiliensis hydrolyzed with Protamex as a promising source of anti-Candida peptides for pharmaceutical and nutraceutical applications. Full article
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