Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
6.1
3.3
Journals
Membranes
Special Issues
Design and Characterization of Membranes for Biomedical Applications
share announcement

Design and Characterization of Membranes for Biomedical Applications

A special issue of Membranes (ISSN 2077-0375). This special issue belongs to the section "Membrane Applications for Other Areas".

Deadline for manuscript submissions: 31 August 2024 | Viewed by 1051

Special Issue Editor

Dr. Metin Uz

E-Mail Website
Guest Editor
Chemical and Biomedical Engineering, Cleveland State University, Cleveland, OH 44115-2214, USA
Interests: polymeric membranes; flexible electronics; biomaterials; 3D printed scaffolds/hydrogels; neural tissue engineering; drug/gene delivery for cancer treatment
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

The development of membranes for biomedical applications has been receiving growing interest. The membranes obtained from different materials (i.e., polymeric, inorganic, composite, etc.) using different fabrication/modification methods (i.e., phase separation, layer-by layer modification, etc.) have the potential to enable enhanced performance in controlling the flux, separation, permselectivity, antifouling, or controlled release properties. Therefore, membranes formulated using different materials and fabrication/modification methods could be used in different biomedical applications to address various challenges, such as hemodialysis, wound healing, bioseparations, and so on. However, there is still a need to develop novel membrane technologies and gain mechanistic and structural understanding to further improve this technology and its potential use in various biomedical applications.

This Special Issue specifically focuses on the “Design and Characterization of Membranes for Biomedical Applications”, including, but not limited to, hemodialysis, protein and ion separation, therapeutic/active molecule isolation and purification, wound healing, tissue regeneration, and controlled drug release. This Special Issue expects to receive contributions in the form of original research papers sharing the latest results and review articles demonstrating the state-of-the-art technology and future directions on membranes for biomedical applications. Topics may include, but are not limited to, novel material-based membrane development and characterization, composite membranes, surface-modified membranes, novel manufacturing/modification techniques, electrically conductive membranes, and the economic feasibility of membrane technologies addressing these biomedical applications.

Dr. Metin Uz
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Membranes is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • polymeric membranes
  • composite membranes
  • controlled release
  • bioseparation
  • biomedical applications

Published Papers (1 paper)

Order results
Result details
Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:

Research

15 pages, 2161 KiB  
Article
Efficient Isolation of Outer Membrane Vesicles (OMVs) Secreted by Gram-Negative Bacteria via a Novel Gradient Filtration Method
by Ning Li, Minghui Wu, Lu Wang, Mengyu Tang, Hongbo Xin and Keyu Deng
Membranes 2024, 14(6), 135; https://doi.org/10.3390/membranes14060135 - 6 Jun 2024
Viewed by 831
Abstract
Bacterial extracellular vesicles (bEVs) secreted by Gram-negative bacteria are referred to as outer membrane vesicles (OMVs) because they originate in the outer membrane. OMVs are membrane-coated vesicles 20–250 nm in size. They contain lipopolysaccharide (LPS), peptidoglycan, proteins, lipids, nucleic acids, and other substances [...] Read more.
Bacterial extracellular vesicles (bEVs) secreted by Gram-negative bacteria are referred to as outer membrane vesicles (OMVs) because they originate in the outer membrane. OMVs are membrane-coated vesicles 20–250 nm in size. They contain lipopolysaccharide (LPS), peptidoglycan, proteins, lipids, nucleic acids, and other substances derived from their parent bacteria and participate in the transmission of information to host cells. OMVs have broad prospects in terms of potential application in the fields of adjuvants, vaccines, and drug delivery vehicles. Currently, there remains a lack of efficient and convenient methods to isolate OMVs, which greatly limits OMV-related research. In this study, we developed a fast, convenient, and low-cost gradient filtration method to separate OMVs that can achieve industrial-scale production while maintaining the biological activity of the isolated OMVs. We compared the gradient filtration method with traditional ultracentrifugation to isolate OMVs from probiotic Escherichia coli Nissle 1917 (EcN) bacteria. Then, we used RAW264.7 macrophages as an in vitro model to study the influence on the immune function of EcN-derived OMVs obtained through the gradient filtration method. Our results indicated that EcN-derived OMVs were efficiently isolated using our gradient filtration method. The level of OMV enrichment obtained via our gradient filtration method was about twice as efficient as that achieved through traditional ultracentrifugation. The EcN-derived OMVs enriched through the gradient filtration method were successfully taken up by RAW264.7 macrophages and induced them to secrete pro-inflammatory cytokines such as tumor necrosis factor α (TNF-α) and interleukins (ILs) 6 and 1β, as well as anti-inflammatory cytokine IL-10. Furthermore, EcN-derived OMVs induced more anti-inflammatory response (i.e., IL-10) than pro-inflammatory response (i.e., TNF-α, IL-6, and IL-1β). These results were consistent with those reported in the literature. The related literature reported that EcN-derived OMVs obtained through ultracentrifugation could induce stronger anti-inflammatory responses than pro-inflammatory responses in RAW264.7 macrophages. Our simple and novel separation method may therefore have promising prospects in terms of applications involving the study of OMVs. Full article
(This article belongs to the Special Issue Design and Characterization of Membranes for Biomedical Applications)
►▼ Show Figures

Figure 1

Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:
 
Displaying articles 1-1
Back to TopTop