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Advanced Chromatographic Methodologies for the Analysis of Natural and Bioactive...
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Advanced Chromatographic Methodologies for the Analysis of Natural and Bioactive Compounds

A special issue of Molecules (ISSN 1420-3049). This special issue belongs to the section "Analytical Chemistry".

Deadline for manuscript submissions: closed (30 June 2024) | Viewed by 4805

Special Issue Editor

Dr. Zeineb Aturki

E-Mail Website
Guest Editor
Institute for Biological Systems (ISB), Consiglio Nazionale delle Ricerche, Area della Ricerca di Roma I, Via Salaria Km 29.300, 00015 Rome, Italy
Interests: miniaturized separation techniques; liquid chromatography; mass spectrometry; food and natural products extracts characterization; bioactive compounds; polyphenols; contaminants
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

The analysis of natural and bioactive compounds has witnessed a remarkable transformation in recent years, owing to significant advancements in chromatographic techniques. These compounds, found in medicinal plants, phytochemicals, functional foods, and supplements, have captured the attention of researchers across various scientific disciplines. Their applications in fields as diverse as pharmaceuticals, agriculture, environmental science, and food chemistry have spurred the development of novel analytical approaches.

Chromatography has long been a cornerstone in the field of analytical chemistry, enabling the separation, identification, and quantification of a wide range of compounds. In recent years, the development of advanced chromatographic methodologies has revolutionized the analysis of natural and bioactive compounds. These compounds, which include phytochemicals, natural products, and biologically active substances, play crucial roles in various scientific fields.

In this context, the Special Issue titled "Advanced Chromatographic Methodologies for the Analysis of Natural and Bioactive Compounds" seeks to explore and celebrate these groundbreaking developments. We welcome submissions that focus on the innovative application of advanced chromatographic techniques in the analysis of natural and bioactive compounds in real matrices. The primary objective of this Special Issue is to provide a platform for researchers to present their original works in developing novel analytical methodologies using state-of-the-art chromatographic instruments. This includes but is not limited to, techniques such as UPLC (ultra-performance liquid chromatography) with high-resolution detectors, nano-liquid chromatography, capillary chromatography, and gas chromatography.

Dr. Zeineb Aturki
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Molecules is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • chromatographic methodologies
  • bioactive compounds
  • UPLC
  • capillary chromatography
  • nano-liquid chromatography
  • gas chromatography

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Published Papers (3 papers)

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Research

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20 pages, 2434 KiB  
Article
Comparative Study of Gas and Liquid Chromatography Methods for the Determination of Underivatised Neutral and Acidic Cannabinoids and Cholesterol
by Marian Czauderna, Tomáš Taubner and Wiktoria Wojtak
Molecules 2024, 29(10), 2165; https://doi.org/10.3390/molecules29102165 - 7 May 2024
Viewed by 1010
Abstract
The aim of our study was to develop a gas chromatographic method coupled with mass spectrometry (GC-MS) for the determination of underivatised neutral (CBDs-N) and acidic (CBDs-A) cannabinoids (CBDs) and cholesterol (Chol). Emphasis was also placed on comparing our original GC-MS method with [...] Read more.
The aim of our study was to develop a gas chromatographic method coupled with mass spectrometry (GC-MS) for the determination of underivatised neutral (CBDs-N) and acidic (CBDs-A) cannabinoids (CBDs) and cholesterol (Chol). Emphasis was also placed on comparing our original GC-MS method with the currently developed C18-high-performance liquid chromatography with photodiode detection (C18-HPLC-DAD). A combination of a long GC column, shallow temperature column programme, and mass-spectrometry was employed to avoid issues arising from the overlap between CBDs and Chol and background fluctuations. The pre-column procedure for CBDs and Chol in egg yolks consisted of hexane extractions, whereas the pre-column procedure for CBDs in non-animal samples involved methanol and hexane extractions. CBDs-A underwent decarboxylation to CBDs during GC-MS analyses, and pre-column extraction of the processed sample with NaOH solution allowed for CBD-A removal. No losses of CBDs-N were observed in the samples extracted with NaOH solution. GC-MS analyses of the samples before and after extraction with NaOH solution enabled the quantification of CBDs-A and CBDs-N. CBDs-A did not undergo decarboxylation to CBDs-N during C18-HPLC-DAD runs. The use of the C18-HPLC-DAD method allowed simultaneous determination of CBDs-N and CBDs-A. In comparison to the C18-HPLC-DAD method, our GC-MS technique offered improved sensitivity, precision, specificity, and satisfactory separation of underivatised CBDs and Chol from biological materials of endogenous species, especially in hemp and hen egg yolk. The scientific novelty of the present study is the application of the GC-MS method for quantifying underivatised CBDs-A, CBDs-N, and Chol in the samples of interest. Full article
(This article belongs to the Special Issue Advanced Chromatographic Methodologies for the Analysis of Natural and Bioactive Compounds)
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13 pages, 3127 KiB  
Article
Quantitative Detection of Thitsiol and Urushiol as Markers from the Gluta usitata Lacquer Tree Using HPLC
by Youngseo Lee, Jihye Lee, Kang-Bong Lee, Won-Yong Lee and Yeonhee Lee
Molecules 2024, 29(1), 149; https://doi.org/10.3390/molecules29010149 - 26 Dec 2023
Viewed by 1308
Abstract
Lacquer sap has been traditionally used in coatings and artwork. Suitable types of lacquer are required to preserve and restore artifacts. Recently, unsuitable cashew nut shell liquid (CNSL) has often been mixed with lacquer sap, so it is necessary to identify the characteristics [...] Read more.
Lacquer sap has been traditionally used in coatings and artwork. Suitable types of lacquer are required to preserve and restore artifacts. Recently, unsuitable cashew nut shell liquid (CNSL) has often been mixed with lacquer sap, so it is necessary to identify the characteristics of lacquer sap by the production area. However, research is still focused on urushiol and laccol. In this study, Myanmarese lacquer sap collected from Gluta usitata, which contains thitsiol as the main component, was analyzed by HPLC to quantify thitsiol using the standards 3-(10-phenyldecyl) benzene-1,2-diol (thitsiol 16) and 3-(8Z,11Z-pentadecadienyl)-benzenediol (urushiol 15:2) as markers, and calibration curves were plotted. The coefficients of determination (R2) for thitsiol 16 and urushiol 15:2 were 0.9985 and 0.9983, respectively. In addition, a blind test was conducted to confirm that accurate quantitative analysis was possible even when Myanmarese lacquer was mixed with lacquer from another production area, which contained urushiol as the main component, and CNSL, which contained cardol, a completely different catechol. Quantitative analysis of thitsiol 16 and urushiol 15:2 in Myanmarese lacquer using HPLC can be used to evaluate the quality of lacquer sap and for more sophisticated activities such as restoration by classifying differences in lacquer sap by the production area. Full article
(This article belongs to the Special Issue Advanced Chromatographic Methodologies for the Analysis of Natural and Bioactive Compounds)
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Review

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34 pages, 1642 KiB  
Review
What Are We Eating? Surveying the Presence of Toxic Molecules in the Food Supply Chain Using Chromatographic Approaches
by Natalia Casado, Cristina V. Berenguer, José S. Câmara and Jorge A. M. Pereira
Molecules 2024, 29(3), 579; https://doi.org/10.3390/molecules29030579 - 24 Jan 2024
Cited by 2 | Viewed by 1964
Abstract
Consumers in developed and Western European countries are becoming more aware of the impact of food on their health, and they demand clear, transparent, and reliable information from the food industry about the products they consume. They recognise that food safety risks are [...] Read more.
Consumers in developed and Western European countries are becoming more aware of the impact of food on their health, and they demand clear, transparent, and reliable information from the food industry about the products they consume. They recognise that food safety risks are often due to the unexpected presence of contaminants throughout the food supply chain. Among these, mycotoxins produced by food-infecting fungi, endogenous toxins from certain plants and organisms, pesticides, and other drugs used excessively during farming and food production, which lead to their contamination and accumulation in foodstuffs, are the main causes of concern. In this context, the goals of this review are to provide a comprehensive overview of the presence of toxic molecules reported in foodstuffs since 2020 through the Rapid Alert System for Food and Feed (RASFF) portal and use chromatography to address this challenge. Overall, natural toxins, environmental pollutants, and food-processing contaminants are the most frequently reported toxic molecules, and liquid chromatography and gas chromatography are the most reliable approaches for their control. However, faster, simpler, and more powerful analytical procedures are necessary to cope with the growing pressures on the food chain supply. Full article
(This article belongs to the Special Issue Advanced Chromatographic Methodologies for the Analysis of Natural and Bioactive Compounds)
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