Polymer Applications in Cell Cultures, Cell Behavior, and Tissue Engineering

A special issue of Polymers (ISSN 2073-4360). This special issue belongs to the section "Polymer Applications".

Deadline for manuscript submissions: closed (31 July 2023) | Viewed by 3340

Special Issue Editor


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Guest Editor
Faculty of Biology, M.V. Lomonosov Moscow State University, Leninskie Gory 1-12, 119234 Moscow, Russia
Interests: alginates; polyhydroxyalkanoates; biodegradation; biosynthesis; poly(3-hydroxybutyrate); bioengineering; biotechnology; biochemical; biocompatibility; stem cells; spheroids; proliferation; scaffolds; medical; modeling; morphology; microbiota

Special Issue Information

Dear Colleagues,

Polymer surface properties, which affect the adhesion, proliferation, migration, differentiation, and functional activity of mammalian cells (mature specialized cells, embryonic and mesenchymal stem cells, cancer cells) and bacteria (probiotic and pathogenic), have promising applications in cell biology and bioengineering. The nano- and microstructure of natural substances (first of all, biopolymers) that form various surfaces of mammalian organs, in combination with their physicochemical properties, play a significant role in the regulation of cell behavior. In biomedical applications, the polymer surface is more than a “passive” substrate for cell attachment and proliferation. Various nano- and microtopographical features (pores, pits, pillars, grooves, ridges, wrinkles etc.) of films, membranes, scaffolds, implants, and devices, as well as the presence of functional groups, energy, charges, hydrophobicity/hydrophilicity, surface rigidity, etc., can impact cell functionality through, for example, altering the cell proliferation rate or stimulating their differentiation. This Special Issue aims to highlight the relationship between surface properties and cell behavior and its potential for biomedical applications.

Dr. Vera Voinova
Guest Editor

Manuscript Submission Information

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Keywords

  • polymer
  • surface
  • topography
  • nanotopography
  • microtopography
  • microstructure
  • nanostructure
  • physicochemical property
  • stem cells
  • cancer cells
  • bacteria
  • adhesion
  • proliferation
  • migration
  • differentiation
  • pores
  • pits
  • pillars
  • grooves
  • roughness

Published Papers (2 papers)

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Research

19 pages, 3694 KiB  
Article
Biocompatibility of 3D-Printed PLA, PEEK and PETG: Adhesion of Bone Marrow and Peritoneal Lavage Cells
by Stanislav Y. Shilov, Yulia A. Rozhkova, Lubov N. Markova, Mikhail A. Tashkinov, Ilya V. Vindokurov and Vadim V. Silberschmidt
Polymers 2022, 14(19), 3958; https://doi.org/10.3390/polym14193958 - 22 Sep 2022
Cited by 13 | Viewed by 2837
Abstract
Samples in the form of cylindrical plates, additively manufactured using the fused deposition modelling (or filament freeform fabrication, FDM/FFF) technology from polylactide (PLA), polyethylene terephthalate glycol (PETG) and polyetheretherketone (PEEK), were studied in series of in-vitro experiments on the adhesion of rat bone-marrow [...] Read more.
Samples in the form of cylindrical plates, additively manufactured using the fused deposition modelling (or filament freeform fabrication, FDM/FFF) technology from polylactide (PLA), polyethylene terephthalate glycol (PETG) and polyetheretherketone (PEEK), were studied in series of in-vitro experiments on the adhesion of rat bone-marrow cells and rat peritoneal cells. Methods of estimation of the absolute number of cells and polymer samples’ mass change were used for the evaluation of cells adhesion, followed by the evaluation of cell-culture supernatants. The results of experiments for both types of cells demonstrated a statistically significant change in the absolute number of cells (variation from 44 to 119%) and the weight of the polymer samples (variation from 0.61 to 2.18%), depending on roughness of sample surface, controlled by a nozzle diameter of a 3D printer as well as printing layer height. It was found that more cells adhere to PLA samples with a larger nozzle diameter and layer height. For PETG samples, the results did not show a clear relationship between cell adhesion and printing parameters. For PEEK samples, on the contrary, adhesion to samples printed with a lower nozzle diameter (higher resolution) is better than to samples printed with a larger nozzle diameter (lower resolution). The difference in results for various polymers can be explained by their chemical structure. Full article
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23 pages, 13314 KiB  
Article
Honeycomb-Structured Porous Films from Poly(3-hydroxybutyrate) and Poly(3-hydroxybutyrate-co-3-hydroxyvalerate): Physicochemical Characterization and Mesenchymal Stem Cells Behavior
by Viktoryia I. Kulikouskaya, Viktoryia V. Nikalaichuk, Anton P. Bonartsev, Elizaveta A. Akoulina, Nikita V. Belishev, Irina V. Demianova, Dariana V. Chesnokova, Tatiana K. Makhina, Garina A. Bonartseva, Konstantin V. Shaitan, Kseniya S. Hileuskaya and Vera V. Voinova
Polymers 2022, 14(13), 2671; https://doi.org/10.3390/polym14132671 - 30 Jun 2022
Cited by 3 | Viewed by 1795
Abstract
Surface morphology affects cell attachment and proliferation. In this research, different films made of biodegradable polymers, poly(3-hydroxybutyrate) (PHB) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHB-co-HV), containing different molecular weights, with microstructured surfaces were investigated. Two methods were used to obtain patterned films—water-assisted self-assembly [...] Read more.
Surface morphology affects cell attachment and proliferation. In this research, different films made of biodegradable polymers, poly(3-hydroxybutyrate) (PHB) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHB-co-HV), containing different molecular weights, with microstructured surfaces were investigated. Two methods were used to obtain patterned films—water-assisted self-assembly (“breath figure”) and spin-coating techniques. The water-assisted technique made it possible to obtain porous films with a self-assembled pore structure, which is dependent on the monomer composition of a polymer along with its molecular weight and the technique parameters (distance from the nozzle, volume, and polymer concentration in working solution). Their pore morphologies were evaluated and their hydrophobicity was examined. Mesenchymal stem cells (MSCs) isolated from bone marrow were cultivated on a porous film surface. MSCs’ attachment differed markedly depending on surface morphology. On strip-formed stamp films, MSCs elongated along the structure, however, they interacted with a larger area of film surface. The honeycomb films and column type films did not set the direction of extrusion, but cell flattening depended on structure topography. Thus, stem cells can “feel” the various surface morphologies of self-assembled honeycomb films and change their behavior depending on it. Full article
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