Application of Separations Technologies in Biomedical Analysis

A special issue of Separations (ISSN 2297-8739). This special issue belongs to the section "Bioanalysis/Clinical Analysis".

Deadline for manuscript submissions: closed (31 July 2022) | Viewed by 3966

Special Issue Editor


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Guest Editor
The Royal Institute of Technology, Stockholm, Sweden
Interests: proteomics; mass spectrometry; protein profiling; longitudinal sampling; internal standards; assay development; microsampling; metabolomics; integrative omics; precision medicine

Special Issue Information

Dear Colleagues,

This Special Issue invites contributions to the current advances in the development and application of analytical methods for biomedical analysis aimed toward biological fluids or bodily fluids. This liquid class of specimens includes any liquids that can be found within the human body, such as urine, blood, or saliva. Bodily fluids are often extraordinarily complex in nature and mainly consist of salts, proteins, metabolites, and lipids, but they also contain other biomolecules carrying important information about the patient. Separation of these biomolecules prior to any analysis remains one of the most crucial components in today’s biomedical analysis and affects the downstream assay performance used to assess at what levels these molecules are present. Separation of these molecules can be performed at different levels to achieve a reduced complexity, ranging from upfront enrichment using nanoparticles or antibodies to alternative ion-sampling strategies when analyzing the sample using methods such as LC-MS/MS.

The target of this Special Issue is to present state-of-the-art separation methods applied to biological fluids, which enable sensitive detection or robust quantification suitable for clinical applications.

With your rich expertise in the field of biochemical separation, we believe that your contribution to this Special Issue will be of great importance to the scientific community.

Dr. Fredrik Edfors

Manuscript Submission Information

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Keywords

  • Blood plasma/serum
  • urine
  • saliva
  • protein profile
  • proteomics
  • metabolite profile
  • metabolomics
  • mass-spectrometry

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Published Papers (1 paper)

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Research

8 pages, 616 KiB  
Article
HILIC-MS/MS Analysis of Adenosine in Patient Blood
by Christina Virgiliou, Nikolaos Fragakis, Melani Sotiriadou, Vassilios Vassilikos, Spiros Gerou, Georgios Theodoridis and Helen Gika
Separations 2021, 8(11), 222; https://doi.org/10.3390/separations8110222 - 17 Nov 2021
Cited by 5 | Viewed by 3485
Abstract
Adenosine is a purine ribonucleoside with important roles in various physiological processes. A number of studies have indicated the importance of adenosine in cardiovascular diseases including syncope; however, the accurate determination of adenosine in human blood is challenging due to the molecule’s instability. [...] Read more.
Adenosine is a purine ribonucleoside with important roles in various physiological processes. A number of studies have indicated the importance of adenosine in cardiovascular diseases including syncope; however, the accurate determination of adenosine in human blood is challenging due to the molecule’s instability. In the present study, we report a simple method for the pre-treatment of blood samples and the development of a fast and efficient hydrophilic interaction chromatographic tandem mass spectrometry method for the analysis of adenosine in patient blood. During collection, samples were mixed directly with a solvent mixture containing 95% acetonitrile and 10 mM ammonium formate in a Vacutainer tube, resulting in successful prevention of adenosine metabolic processes and direct blood sample deproteinization. The method was validated according to bioanalytical industry guidelines and found to be accurate, repeatable, specific and sensitive with LLOQ 0.005 μg/mL, thus allowing its application in the analysis of real clinical samples. Full article
(This article belongs to the Special Issue Application of Separations Technologies in Biomedical Analysis)
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