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Separations
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HPLC or Other Chromatographic-Based Methods for Analysis of Bioactive Compounds...
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HPLC or Other Chromatographic-Based Methods for Analysis of Bioactive Compounds in Plant, Food, and Pharmaceutical Products

A special issue of Separations (ISSN 2297-8739). This special issue belongs to the section "Analysis of Natural Products and Pharmaceuticals".

Deadline for manuscript submissions: closed (10 May 2024) | Viewed by 3669

Special Issue Editor

Prof. Dr. Yang-Bong Lee

E-Mail Website
Guest Editor
Department of Food Science and Technology, Pukyong National University, Busan 48513, Republic of Korea
Interests: isolation; separation; purification of bioactive compounds

Special Issue Information

Dear Colleagues,

Bioactive compounds are substances that enhance or inhibit the functions of living organisms, playing a role in correcting abnormal conditions due to deficiencies in or the excessive secretion of substances regulating functions in the living body. Therefore, the creation of physiologically active substances is very important for helping modern people to lead a better and healthier life, and extensive research has been conducted on this subject recently. New biologically active substances can be obtained from natural products such as animals and plants, extracted and purified from metabolites of microorganisms and animal and plant cell lines, or obtained through chemical synthesis. There are various pretreatment methods available to separate and purify these bioactive substances, and HPLC is a representative analytical method.

In this Issue, we would like to introduce various pretreatment methods for extraction and purification of bioactive substances from plant, food or pharmaceuticals and analysis methods using HPLC and other chromatography.

Prof. Dr. Yang-Bong Lee
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Separations is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • bioactive compounds
  • physiologically active substances
  • medicinal substances
  • natural products
  • metabolites
  • pretreatment methods
  • extraction, isolation, separation and purification
  • plant, food and pharmaceutical compounds
  • analytical methods
  • HPLC
  • other chromatographic technologies

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Published Papers (2 papers)

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Research

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14 pages, 2306 KiB  
Article
HPLC/DAD Analysis and Antioxidant Activity of Adlay Sprouts and Seeds
by Gia-Han Tran, Neil-Patrick Uy, Shi-Heon Kang, Wonseok Heo, Eun-Song Lee, Seong-Soo Roh and Sanghyun Lee
Separations 2024, 11(1), 32; https://doi.org/10.3390/separations11010032 - 13 Jan 2024
Cited by 3 | Viewed by 1938
Abstract
Adlay is an annual plant known for its abundant bioactive compounds and diverse pharmacological activities. Coixol, a key component found in various parts of adlay, significantly contributes to its biological activity. This study was conducted to extract adlay sprouts and seeds using different [...] Read more.
Adlay is an annual plant known for its abundant bioactive compounds and diverse pharmacological activities. Coixol, a key component found in various parts of adlay, significantly contributes to its biological activity. This study was conducted to extract adlay sprouts and seeds using different solvents (methanol and ethanol) and extraction methods (reflux and ultrasonic extraction). The extracts were then evaluated for their total polyphenol and flavonoid contents, as well as antioxidant ability (DPPH and ABTS+). Additionally, the coixol content of these extracts was analyzed using HPLC/DAD analysis. The results showed that the extraction methods and solvents used impacted the bioactive compounds and their activities in the samples. Adlay sprouts exhibited a higher compound content and stronger antioxidant capacity than adlay seeds. Moreover, a substantial amount of coixol was found in the sprouts, while it was not detected in the seeds. This study emphasizes the importance of selecting appropriate extraction methods to optimize the biological activities of adlay sprouts and seeds. Adlay sprouts, with their enriched phytochemical compounds and enhanced antioxidant ability, could serve as a valuable material for health product applications. Full article
(This article belongs to the Special Issue HPLC or Other Chromatographic-Based Methods for Analysis of Bioactive Compounds in Plant, Food, and Pharmaceutical Products)
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Review

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20 pages, 739 KiB  
Review
Research Progress in the Separation of Chemical Components from Essential Oils by High-Speed Countercurrent Chromatography
by Linhong He, Zihao Zhong, Lijuan Zhang and Xi Bai
Separations 2024, 11(5), 152; https://doi.org/10.3390/separations11050152 - 15 May 2024
Viewed by 1374
Abstract
Essential oils (EOs) are vital secondary metabolites in plants. They have garnered substantial attention owing to their distinct flavors and desirable attributes, including potent antioxidant, antibacterial, and antitumor properties. Nevertheless, the active constituents of EOs exhibit intricate chemical structures, and conventional separation techniques [...] Read more.
Essential oils (EOs) are vital secondary metabolites in plants. They have garnered substantial attention owing to their distinct flavors and desirable attributes, including potent antioxidant, antibacterial, and antitumor properties. Nevertheless, the active constituents of EOs exhibit intricate chemical structures, and conventional separation techniques are inadequate for purifying the individual chemical components from EOs. High-speed countercurrent chromatography, based on the principles of a hydrodynamic equilibrium system, has emerged as a liquid–liquid chromatographic separation method renowned for its ability to handle substantial single injection volumes and the absence of irreversible adsorption. Consequently, in recent years, this technique has been widely employed in the isolation and refinement of natural products. In this review, a comprehensive analysis is conducted, contrasting the merits and demerits of high-speed countercurrent chromatography with conventional separation methods. The solvent systems, elution modes, commonly employed detectors, and practical applications are reviewed in the context of high-speed countercurrent chromatography for essential oil separation and purification. Furthermore, this review offers a glimpse into the potential prospects of applying this technique, with the intention of serving as a valuable reference for the use of high-speed countercurrent chromatography in the purification of EOs. Full article
(This article belongs to the Special Issue HPLC or Other Chromatographic-Based Methods for Analysis of Bioactive Compounds in Plant, Food, and Pharmaceutical Products)
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