Vaccine for Parasitic Diseases

A special issue of Vaccines (ISSN 2076-393X).

Deadline for manuscript submissions: closed (21 July 2023) | Viewed by 1791

Special Issue Editors


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Guest Editor
College of Veterinary Medicine, Iowa State University, Ames, IA, USA
Interests: susceptibility and resistance in helminths of veterinary; anti-parasitic drugs; drug mechanisms of action and resistance; molecular and immunological diagnostic techniques; vaccine development

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Guest Editor
College of Veterinary Medicine, Iowa State University, Ames, IA, USA
Interests: veterinary parasitology

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Guest Editor
Fundacao Oswaldo Cruz, Rio de Janeiro, Brazil
Interests: schistosomiasis; vaccine; Sm14; FABP

Special Issue Information

Dear Colleagues, 

Significant efforts and progress have been made over the last few years in the development of vaccines against parasitic diseases. However, an effective vaccine is not yet available. The difficulties in developing a vaccine against parasitic diseases are complicated not only by the necessity to identify (and produce) appropriate, protective antigens but also a lack of complete understanding of the types of immune responses needed for protection. Despite these hurdles, most scholars are trying to design and develop more advanced vaccines for parasitic diseases. 

We are pleased to invite you to submit your new research on vaccines for parasitic diseases to this Special Issue. This Special Issue focuses on novel and repurposed antiparasitic drugs, drug mechanisms of action and resistance, molecular and immunological diagnostic techniques, vaccine development and parasite–host interactions. 

We look forward to receiving your contributions.

Dr. Jeba Jesudoss Chelladurai
Dr. Matthew Thomas Brewer
Dr. Miriam Tendler
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Vaccines is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Published Papers (1 paper)

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Research

17 pages, 4439 KiB  
Article
Inhibition of Trichinella spiralis Membrane-Associated Progesterone Receptor (MAPR) Results in a Reduction in Worm Burden
by Muhammad Tahir Aleem, Zhaohai Wen, Zhengqing Yu, Cheng Chen, Mingmin Lu, Lixin Xu, Xiaokai Song, Xiangrui Li and Ruofeng Yan
Vaccines 2023, 11(9), 1437; https://doi.org/10.3390/vaccines11091437 - 31 Aug 2023
Cited by 1 | Viewed by 1495
Abstract
Trichinella spiralis (T. spiralis), a nematode parasite, is the major cause of Trichinellosis, a zoonotic disease. A key role of MAPR in the reproductive system is to maintain pregnancy. Previous studies found that antihormone drug design and vaccine therapy of recombinant [...] Read more.
Trichinella spiralis (T. spiralis), a nematode parasite, is the major cause of Trichinellosis, a zoonotic disease. A key role of MAPR in the reproductive system is to maintain pregnancy. Previous studies found that antihormone drug design and vaccine therapy of recombinant protein (rTs-MAPRC2) control T. spiralis infection. The current study investigates the inhibitory effects of different ratios of antibodies against Ts-MAPRC2 on the development of muscle larvae (ML) and newborn larvae (NBL). First, we performed indirect immunofluorescence assays and examined the effects of rTs-MAPRC2-Ab on ML and NBL in vitro as well as in vivo. Afterward, siRNA-Ts-MAPRC2 was transfected into T. spiralis muscle larvae. Following that, Ts-MAPRC2 protein was detected by Western Blotting, and mRNA levels were determined by qPCR. We also assessed whether siRNA-treated NBLs were infective by analyzing muscle larvae burden (MLs). Our results showed that rTs-MAPRC2-Ab greatly inhibited the activity of the Ts-MAPRC2 in ML and NBL of T. spiralis and rTs-MAPRC2-Ab reduced larval infectivity and survival in the host in a dose-dependent manner (1:50, 1:200, 1:800 dilutions). Furthermore, siRNA-Ts-MAPRC2 effectively silenced the Ts-MAPRC2 gene in muscle larvae (ML) in vitro, as well as in newborn larvae (NBL) of T. spiralis in vivo. In addition, siRNA-Ts-MAPRC2 (siRNA180, siRNA419, siRNA559) reduced host larval survival and infectivity significantly. This study, therefore, suggests that Ts-MAPRC2 might be a novel molecular target useful in the development of vaccines against T. spiralis infection. Full article
(This article belongs to the Special Issue Vaccine for Parasitic Diseases)
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