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Viruses
Special Issues
B Cell-Mediated Immunity to Viruses
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B Cell-Mediated Immunity to Viruses

A special issue of Viruses (ISSN 1999-4915). This special issue belongs to the section "Viral Immunology, Vaccines, and Antivirals".

Deadline for manuscript submissions: 19 February 2025 | Viewed by 433

Special Issue Editor

Prof. Dr. Haiyan Zhao

E-Mail Website
Guest Editor
State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, China
Interests: B cell; immune response; emerging viruses

Special Issue Information

Dear Colleagues,

B cell-mediated humoral immune response, also known as antibody-mediated response, plays a critical role in host defense against invading pathogens and is a key determinant for the development of most effective vaccines. Studying broadly neutralizing antibodies and understanding the rules for eliciting broadly neutralizing B cell responses will help antibody-based therapies and vaccine development. In addition, antibody functions are varied, and not all pathogen-specific antibodies benefit the host. Hence, the scope of this topic is related to B cell development, function, epitopes and application in the face of infectious pathogens.

Prof. Dr. Haiyan Zhao
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Viruses is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • broadly neutralizing antibodies
  • protective antibodies
  • non-protective antibodies
  • epitopes
  • epitope-based vaccine design
  • B cell development
  • B cell repertoire

Published Papers (1 paper)

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9 pages, 2647 KiB  
Brief Report
The Generation and Characterization of Monoclonal Antibodies against the MPXV A29L Protein
by Wenlong Zhu, Mengjia Zhang, Mengdi Zhang, Ran Jing, Jiaru Zhou, Hua Cao, Changcheng Liu, Hongmei Zhu, Ahmed H. Ghonaim, Sherin R. Rouby and Wentao Li
Viruses 2024, 16(8), 1184; https://doi.org/10.3390/v16081184 - 24 Jul 2024
Viewed by 132
Abstract
Mpox (formerly known as monkeypox) is a zoonotic disease caused by monkeypox virus (MPXV), a DNA virus belonging to the Orthopoxvirus genus, in the Poxviridae family. The disease constitutes a moderate risk to public health at the global level. The MPXV A29L protein [...] Read more.
Mpox (formerly known as monkeypox) is a zoonotic disease caused by monkeypox virus (MPXV), a DNA virus belonging to the Orthopoxvirus genus, in the Poxviridae family. The disease constitutes a moderate risk to public health at the global level. The MPXV A29L protein plays a crucial role in coordinating virion assembly and facilitating important virus-host interactions. This study focused on the expression, purification, and recombinant protein synthesis of the A29L protein of MPXV using prokaryotic systems. Using hybridoma technology, we successfully generated the monoclonal antibodies (mAbs) 1E12 and 4B2, which specifically recognize the A29L protein. These mAbs were found to be suitable for use in indirect immunofluorescence assays (IFA), Western blotting, and immunoprecipitation (IP). Our investigation also revealed that mAbs 1E12 and 4B2 could detect the A27L protein, a homologous protein found in the vaccinia virus Western Reserve (VACV WR) strain, using IFA, Western blotting, and immunoprecipitation (IP). Using mAbs 1E12 and 4B2 as primary immunological probes, A27L protein expression was detected as early as 6 h postinfection with VACV WR, with increasing protein levels being observed throughout the infection. This study enhances our understanding of the protein structure and function of MPXV and contributes to the development of specific MPXV detection methods. Full article
(This article belongs to the Special Issue B Cell-Mediated Immunity to Viruses)
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