Immunoreactivity of Polish Lyme Disease Patient Sera to Specific Borrelia Antigens—Part 1
Abstract
:1. Introduction
2. Materials and Methods
2.1. The Study Group
2.2. ELISA Assay
2.3. Immunoblot Assay
2.4. Statistical Analysis
3. Results
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Consent Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Serological Test | Result | Experimental Group n = 80 | Control Group n = 22 | ||||
---|---|---|---|---|---|---|---|
IgM n (%) | IgG n (%) | IgM and IgG n (%) | IgM n (%) | IgG n (%) | IgM and IgG n (%) | ||
ELISA | Positive/borderline | 26 (32.5) | 11 (13.7) | 34 (42.5) | 2 (9.1) | 2 (9.1) | 0 (0.0) |
IB | Positive/borderline | 12 (15.0) | 26 (32.5) | 27 (33.7) | 1 (4.5) | 1 (4.5) | 0 (0.0) |
Class of Antibody/No. of Patients | Patient No. | ELISA | Immunoblot |
---|---|---|---|
IgM n = 5 | No. 24 | (-) | p41; OspC-adv B.a; OspC-adv B.b |
No. 40 | (-) | p41; OspC-adv B.a | |
No. 44 | (-) | p39 B.a; OspC-adv B.a; OspC-adv B.b | |
No. 51 | (-) | p41; OspC-adv B.a | |
No. 68 | (-) | p41; OspC-adv B.a | |
IgG n = 15 | No. 9 | (-) | VlsE B.g; p41; p25 (OspC); p21 |
No. 10 | (-) | p41; p25 (OspC) | |
No.25 | (-) | p83 B.a; p41; p18 | |
No. 30 | (-) | VlsE B.a; p41; p58; p21; p18 | |
No. 36 | (-) | p83; p41 | |
No. 37 | (-) | p41; p39; p25 (OspC) | |
No. 43 | (-) | p41; p39; p25 (OspC); p19 | |
No. 51 | (-) | p41; p39; p25 (OspC) | |
No. 53 | (-) | p41; p39; p25 (OspC) | |
No. 56 | (-) | VlsE B.b; p41 | |
No. 60 | (-) | VlsE B.b; p41 | |
No. 62 | (-) | p21 | |
No. 65 | (-) | VlsE B.g; p83; p39 | |
No. 75 | (-) | p83; p41; p39; p25 (OspC) | |
No. 79 | (-) | Vls E B.g; VlsE B.a; p41; p39; p58 |
Number of Genospecies | Genospecies | Experimental Group | Control Group | ||
---|---|---|---|---|---|
IgM n (%) | IgG n (%) | IgM n (%) | IgG n (%) | ||
1 | B.burgdorferi | 0 (0.0) | 6 (11.3) | 0 (0.0) | 1 (50.0) |
B. garinii | 3 (7.6) | 7 (13.2) | 0 (0.0) | 0 (0.0) | |
B. afzelii | 4 (10.3) | 1 (1.9) | 0 (0.0) | 0 (0.0) | |
2 | B.burgdorferi B. garinii | 0 (0.0) | 7 (13.2) | 0 (0.0) | 0 (0.0) |
B.burgdorferi B. afzelii | 4 (10.3) | 1 (1.9) | 1 (100) | 0 (0.0) | |
B. garinii B. afzelii | 4 (10.3) | 3 (5.7) | 0 (0.0) | 0 (0.0) | |
3 | B. burgdorferi B. garinii B. afzelii | 24 (61.5) | 15 (28.3) | 0 (0.0) | 0 (0.0) |
Antigens common to all genospecies | 0 (0.0) | 13 (24.5) | 0 (0.0) | 1 (50.0) | |
Total | 39 (100) | 53 (100) | 1 (100) | 2 (100) |
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Wojciechowska-Koszko, I.; Mnichowska-Polanowska, M.; Kwiatkowski, P.; Roszkowska, P.; Sienkiewicz, M.; Dołęgowska, B. Immunoreactivity of Polish Lyme Disease Patient Sera to Specific Borrelia Antigens—Part 1. Diagnostics 2021, 11, 2157. https://doi.org/10.3390/diagnostics11112157
Wojciechowska-Koszko I, Mnichowska-Polanowska M, Kwiatkowski P, Roszkowska P, Sienkiewicz M, Dołęgowska B. Immunoreactivity of Polish Lyme Disease Patient Sera to Specific Borrelia Antigens—Part 1. Diagnostics. 2021; 11(11):2157. https://doi.org/10.3390/diagnostics11112157
Chicago/Turabian StyleWojciechowska-Koszko, Iwona, Magdalena Mnichowska-Polanowska, Paweł Kwiatkowski, Paulina Roszkowska, Monika Sienkiewicz, and Barbara Dołęgowska. 2021. "Immunoreactivity of Polish Lyme Disease Patient Sera to Specific Borrelia Antigens—Part 1" Diagnostics 11, no. 11: 2157. https://doi.org/10.3390/diagnostics11112157