Electrospun Water-Borne Polyurethane Nanofibrous Membrane as a Barrier for Preventing Postoperative Peritendinous Adhesion
Abstract
:1. Introduction
2. Results
2.1. Preparation and Characterization of the Electrospun NFM
2.2. Mechanical Properties
2.3. In Vitro Degradation
2.4. Cytotoxicity and Cell Attachment Test
2.5. Animal Study
2.5.1. Gross Evaluation
2.5.2. Histology
2.5.3. Range of Motion
2.5.4. Pullout and Breaking Force
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Synthesis and Physicochemical Analyses of WPU
4.3. Preparation of Electrospun NFMs
4.4. Removal of PEO
4.5. Characterization of Electrospun NFMs
4.6. Confirmation of WPU NFM
4.7. Degradation Rate of WPU NFM
4.8. Cytotoxicity and Cell Attachment Test and In Vitro Cell Culture
4.9. Animal Study
4.10. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Groups | Fiber Diameter (nm) | Fiber Diameter after Immersed in PBS 24 h (nm) | Pore Size (µm) |
---|---|---|---|
W1P2 | 362.3 ± 81.4 | 339.3 ± 83.4 | 0.78 ± 0.31 * |
W2P3 | 349.5 ± 63.3 | 324.5 ± 78.1 | 0.84 + 0.31 * |
W1P1 | 369.3 ± 70.2 | 368.7 ± 56.3 | 0.95 ± 0.36 |
W3P2 | 352.4 ± 131.5 | 372.1 ± 159.5 | 1.05 ± 0.44 |
Membrane | Ultimate Tensile Strength (MPa) | Elongation at Break (%) | Young’s Modulus (MPa) |
---|---|---|---|
W3P2BW | 1.00 ± 0.14 | 84.8 ± 22.7 | 0.14 ± 0.03 |
W1P1BW | 0.82 ± 0.09 | 63.6 ± 16.2 | 0.13 ± 0.01 |
W2P3BW | 0.82 ± 0.02 | 41.4 ± 14.8 | 0.11 ± 0.03 |
W1P2BW | 0.79 ± 0.19 | 89.8 ± 25.2 | 0.13 ± 0.04 |
W3P2 | 8.00 ± 1.25 *,#,‡ | 411.69 ± 85.0 *,# | 0.68 ± 0.17 * |
W1P1 | 7.25 ± 0.91 *,§ | 432.4 ± 66.6 *,# | 0.64 ± 0.13 * |
W2P3 | 8.58 ± 0.79 *#,‡ | 445.0 ± 68.8 *,# | 0.85 ± 0.30 *,‡ |
W1P2 | 4.80 ± 1.58 *,§ | 236.5 ± 109.1 * | 0.92 ± 0.37 *,‡ |
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Chen, S.-H.; Chou, P.-Y.; Chen, Z.-Y.; Lin, F.-H. Electrospun Water-Borne Polyurethane Nanofibrous Membrane as a Barrier for Preventing Postoperative Peritendinous Adhesion. Int. J. Mol. Sci. 2019, 20, 1625. https://doi.org/10.3390/ijms20071625
Chen S-H, Chou P-Y, Chen Z-Y, Lin F-H. Electrospun Water-Borne Polyurethane Nanofibrous Membrane as a Barrier for Preventing Postoperative Peritendinous Adhesion. International Journal of Molecular Sciences. 2019; 20(7):1625. https://doi.org/10.3390/ijms20071625
Chicago/Turabian StyleChen, Shih-Heng, Pang-Yun Chou, Zhi-Yu Chen, and Feng-Huei Lin. 2019. "Electrospun Water-Borne Polyurethane Nanofibrous Membrane as a Barrier for Preventing Postoperative Peritendinous Adhesion" International Journal of Molecular Sciences 20, no. 7: 1625. https://doi.org/10.3390/ijms20071625