Mesenchymal Stem Cell-Conditioned Medium Induces Neutrophil Apoptosis Associated with Inhibition of the NF-κB Pathway in Endotoxin-Induced Acute Lung Injury
Abstract
:1. Introduction
2. Results
2.1. Effects of MSC-CM on the Histopathology of Endotoxin-Induced ALI
2.2. Effects of MSC-CM on Proinflammatory Cytokines in Lung Tissues
2.3. Effects of MSC-CM on Neutrophil Accumulation in Lung Tissues
2.4. Effects of MSC-CM on Neutrophil Apoptosis in ALI
2.5. Effects of MSC-CM on the Expression of NF-kB and MMP-9 in Mouse and Human Neutrophils
3. Discussion
4. Materials and Methods
4.1. Mice
4.2. Experimental Design
4.3. Generation of Murine and Human MSC-CM
4.4. Isolation of Neutrophils from BALF
4.5. Histology and IHC of Lung Tissue Sections
4.6. Lung Injury Scoring of Lung Tissue Sections
4.7. Enzyme-Linked Immunosorbent Assay (ELISA) Assay
4.8. Confocal Microscopy
4.9. Myeloperoxidase (MPO) Activity Assay
4.10. Flow Cytometry
4.11. Patients with ARDS and Isolation of Human Neutrophils
4.12. Electrophoretic Mobility Shift Assay (EMSA)
4.13. Apoptosis Assay
4.14. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Availability of Data and Materials
Ethics Approval and Consent to Participate
Conflicts of Interest
References
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Su, V.Y.-F.; Lin, C.-S.; Hung, S.-C.; Yang, K.-Y. Mesenchymal Stem Cell-Conditioned Medium Induces Neutrophil Apoptosis Associated with Inhibition of the NF-κB Pathway in Endotoxin-Induced Acute Lung Injury. Int. J. Mol. Sci. 2019, 20, 2208. https://doi.org/10.3390/ijms20092208
Su VY-F, Lin C-S, Hung S-C, Yang K-Y. Mesenchymal Stem Cell-Conditioned Medium Induces Neutrophil Apoptosis Associated with Inhibition of the NF-κB Pathway in Endotoxin-Induced Acute Lung Injury. International Journal of Molecular Sciences. 2019; 20(9):2208. https://doi.org/10.3390/ijms20092208
Chicago/Turabian StyleSu, Vincent Yi-Fong, Chi-Shiuan Lin, Shih-Chieh Hung, and Kuang-Yao Yang. 2019. "Mesenchymal Stem Cell-Conditioned Medium Induces Neutrophil Apoptosis Associated with Inhibition of the NF-κB Pathway in Endotoxin-Induced Acute Lung Injury" International Journal of Molecular Sciences 20, no. 9: 2208. https://doi.org/10.3390/ijms20092208