Exploration of the Effects of Different Blue LED Light Intensities on Flavonoid and Lipid Metabolism in Tea Plants via Transcriptomics and Metabolomics

Wang, Pengjie; Chen, Sirong; Gu, Mengya; Chen, Xiaomin; Chen, Xuejin; Yang, Jiangfan; Zhao, Feng; Ye, Naixing

doi:10.3390/ijms21134606

Open AccessArticle

Exploration of the Effects of Different Blue LED Light Intensities on Flavonoid and Lipid Metabolism in Tea Plants via Transcriptomics and Metabolomics

by

Pengjie Wang

¹,

Sirong Chen

¹,

Mengya Gu

¹,

Xiaomin Chen

¹,

Xuejin Chen

¹,

Jiangfan Yang

¹,

Feng Zhao

^2,*

and

Naixing Ye

^1,*

¹

College of Horticulture, Fujian Agriculture and Forestry University/Key Laboratory of Tea Science in Universities of Fujian Province, Fuzhou 350002, China

²

College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350002, China

^*

Authors to whom correspondence should be addressed.

Int. J. Mol. Sci. 2020, 21(13), 4606; https://doi.org/10.3390/ijms21134606

Submission received: 10 June 2020 / Revised: 23 June 2020 / Accepted: 25 June 2020 / Published: 29 June 2020

(This article belongs to the Section Molecular Genetics and Genomics)

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Abstract

Blue light extensively regulates multiple physiological processes and secondary metabolism of plants. Although blue light quantity (fluence rate) is important for plant life, few studies have focused on the effects of different blue light intensity on plant secondary metabolism regulation, including tea plants. Here, we performed transcriptomic and metabolomic analyses of young tea shoots (one bud and two leaves) under three levels of supplemental blue light, including low-intensity blue light (LBL, 50 μmol m^–2 s^–1), medium-intensity blue light (MBL, 100 μmol m^–2 s^–1), and high-intensity blue light (HBL, 200 μmol m^–2 s^–1). The total number of differentially expressed genes (DEGs) in LBL, MBL and HBL was 1, 7 and 1097, respectively, indicating that high-intensity blue light comprehensively affects the transcription of tea plants. These DEGs were primarily annotated to the pathways of photosynthesis, lipid metabolism and flavonoid synthesis. In addition, the most abundant transcription factor (TF) families in DEGs were bHLH and MYB, which have been shown to be widely involved in the regulation of plant flavonoids. The significantly changed metabolites that we detected contained 15 lipids and 6 flavonoid components. Further weighted gene co-expression network analysis (WGCNA) indicated that CsMYB (TEA001045) may be a hub gene for the regulation of lipid and flavonoid metabolism by blue light. Our results may help to establish a foundation for future research investigating the regulation of woody plants by blue light.

Keywords: Camellia sinensis; blue light; transcriptomics; metabolomics; WGCNA

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MDPI and ACS Style

Wang, P.; Chen, S.; Gu, M.; Chen, X.; Chen, X.; Yang, J.; Zhao, F.; Ye, N. Exploration of the Effects of Different Blue LED Light Intensities on Flavonoid and Lipid Metabolism in Tea Plants via Transcriptomics and Metabolomics. Int. J. Mol. Sci. 2020, 21, 4606. https://doi.org/10.3390/ijms21134606

AMA Style

Wang P, Chen S, Gu M, Chen X, Chen X, Yang J, Zhao F, Ye N. Exploration of the Effects of Different Blue LED Light Intensities on Flavonoid and Lipid Metabolism in Tea Plants via Transcriptomics and Metabolomics. International Journal of Molecular Sciences. 2020; 21(13):4606. https://doi.org/10.3390/ijms21134606

Chicago/Turabian Style

Wang, Pengjie, Sirong Chen, Mengya Gu, Xiaomin Chen, Xuejin Chen, Jiangfan Yang, Feng Zhao, and Naixing Ye. 2020. "Exploration of the Effects of Different Blue LED Light Intensities on Flavonoid and Lipid Metabolism in Tea Plants via Transcriptomics and Metabolomics" International Journal of Molecular Sciences 21, no. 13: 4606. https://doi.org/10.3390/ijms21134606

APA Style

Wang, P., Chen, S., Gu, M., Chen, X., Chen, X., Yang, J., Zhao, F., & Ye, N. (2020). Exploration of the Effects of Different Blue LED Light Intensities on Flavonoid and Lipid Metabolism in Tea Plants via Transcriptomics and Metabolomics. International Journal of Molecular Sciences, 21(13), 4606. https://doi.org/10.3390/ijms21134606

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Exploration of the Effects of Different Blue LED Light Intensities on Flavonoid and Lipid Metabolism in Tea Plants via Transcriptomics and Metabolomics

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