Twist1 Influences the Expression of Leading Members of the IL-17 Signaling Pathway in HER2-Positive Breast Cancer Cells
Abstract
:1. Introduction
2. Results
2.1. TWIST1 Is Overexpressed in the HER2+ BC Group
2.2. TWIST1 Knockdown Altered the Gene Expression Profile of Signaling Pathways Relevant to BC
2.3. Twist1 Influences Leading Members of the IL-17 Signaling Pathway
3. Discussion
4. Materials and Methods
4.1. Patient Samples
4.2. Cell Culture and Lentiviral Transduction
4.3. Expression Chip Array Data Analysis
4.4. Real-Time Reverse Transcription Polymerase Chain Reaction (RT-qPCR)
4.5. Immunoblotting
4.6. Flow Cytometry
4.7. Light Phase Contrast and Fluorescence Microscopy
4.8. Enzyme-Linked Immunosorbent Assay (ELISA)
4.9. TCGA Analysis
4.10. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Functional Enrichment Analysis * | Gene # | |
---|---|---|
Upregulated | Downregulated | |
EMT/Cell adhesion | ZO3, CLDN8, KTR4 | TUBA3C, FN1, SLUG, HMGA2 |
ECM remodeling/Blood coagulation | IP3R1, PLAT, SERPINA1, PKCA, ITGB3, GNAI2 | HBEGF, PAI1, PAI2 |
Immune response/Th17-derived cytokines | IL23A, IL6, IL8, CCL20, CSF2 |
Clinical Characteristic | n = 46 | % = 100 |
---|---|---|
Age, yr | ||
Early onset (20–50) Late onset (51–80) | 17 29 | 37.0 63.0 |
Female, n | 46 | 100 |
IHC-based molecular classification * | ||
Luminal A Luminal B HER2-enriched Triple Negative | 16 11 8 11 | 34.8 23.9 17.4 23.9 |
Tumor histological type | ||
Invasive ductal carcinoma | 46 | 100 |
Histological grade | ||
Grade 1 Grade 2 Grade 3 | 3 8 35 | 6.5 17.4 76.1 |
TNM staging | ||
I/II III/IV | 31 15 | 67.4 32.6 |
Tumor largest diameter, cm | ||
<2 2–5 >5 | 9 35 2 | 19.6 76.1 4.3 |
Lymph node status, n | ||
Stage 1 (negative) Stage 2 (1–3 LN) Stage 3 (>3 LN) | 17 20 9 | 37.0 43.5 19.6 |
Distant metastasis, n | ||
Presence Absence | 10 36 | 21.7 78.3 |
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Pires, B.R.B.; Binato, R.; Ferreira, G.M.; Corrêa, S.; Du Rocher, B.; Bulzico, D.; Crocamo, S.; dos Santos, E.C.; Lima, L.G.; Abdelhay, E. Twist1 Influences the Expression of Leading Members of the IL-17 Signaling Pathway in HER2-Positive Breast Cancer Cells. Int. J. Mol. Sci. 2021, 22, 12144. https://doi.org/10.3390/ijms222212144
Pires BRB, Binato R, Ferreira GM, Corrêa S, Du Rocher B, Bulzico D, Crocamo S, dos Santos EC, Lima LG, Abdelhay E. Twist1 Influences the Expression of Leading Members of the IL-17 Signaling Pathway in HER2-Positive Breast Cancer Cells. International Journal of Molecular Sciences. 2021; 22(22):12144. https://doi.org/10.3390/ijms222212144
Chicago/Turabian StylePires, Bruno R. B., Renata Binato, Gerson M. Ferreira, Stephany Corrêa, Bárbara Du Rocher, Daniel Bulzico, Susanne Crocamo, Everton Cruz dos Santos, Luize G. Lima, and Eliana Abdelhay. 2021. "Twist1 Influences the Expression of Leading Members of the IL-17 Signaling Pathway in HER2-Positive Breast Cancer Cells" International Journal of Molecular Sciences 22, no. 22: 12144. https://doi.org/10.3390/ijms222212144