Effect of Clinically Used Microtubule Targeting Drugs on Viral Infection and Transport Function
Abstract
:1. Introduction
2. Results
2.1. Effect of MTAs in Viral Infection
2.2. Effect of MTAs in the Movement of Cellular Motors
2.3. Biochemical and Structural Characterization of Benzimidazoles Complexes with Mammalian Tubulin
3. Discussion
3.1. MTAs Affect Viral Infections and the Effect Varies According to the Virus Dependency on the Microtubular Network
3.2. MDAs Are More Effective than MSAs in Disrupting Cytosolic Trafficking and Viral Infection
4. Materials and Methods
4.1. Proteins and Chemicals
4.2. Peptide Synthesis and Labeling
4.3. Cell Lines and Cultured Conditions
4.4. Viruses
4.5. In Cell Motility Assays
4.6. Drug Treatment and Cell Viability Determination
4.7. Infection Assays
4.8. Crystallization and Crystal Structure Determination
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Huh-7 Lunet C3 | Vero E6 | Vero | A549-ACE2 | |
---|---|---|---|---|
paclitaxel (PTX) | 5 | 25 | 25 | 0.025 |
docetaxel (DTX) | 0.03 | 10 | 10 | 0.0125 |
cabazitaxel (CTX) | 0.01 | 25 | 10 | 0.5 |
ixabepilone (EPO) | 0.05 | 25 | 25 | 0.0125 |
colchicine (COL) | 0.2 | 25 | 75 | 0.1 |
noscapine (NOS) | 100 | 100 | 100 | 50 |
phodophyllotoxin (PPT) | 0.2 | 50 | 50 | 0.1 |
mebendazole (MBZ) | 5 | 10 | 10 | 2 |
albendazole (ABZ) | 1 | 25 | 25 | 2 |
febendazole (FBZ) | 1 | 50 | 75 | 2 |
plinabulin (PLIN) | 0.5 | 25 | 25 | 0.002 |
vinblastine (VBL) | 0.05 | 5 | 25 | 100 |
vincristine (VCR) | 5 | 1 | 1 | 0.4 |
vindesine (VDS) | 0.02 | 100 | 100 | 0.006 |
eribulin (ERIB) | 0.01 | 10 | 100 | 0.0025 |
maytansine (MAYT) | 0.1 | 25 | 25 | 0.02 |
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Oliva, M.Á.; Tosat-Bitrián, C.; Barrado-Gil, L.; Bonato, F.; Galindo, I.; Garaigorta, U.; Álvarez-Bernad, B.; París-Ogáyar, R.; Lucena-Agell, D.; Giménez-Abián, J.F.; et al. Effect of Clinically Used Microtubule Targeting Drugs on Viral Infection and Transport Function. Int. J. Mol. Sci. 2022, 23, 3448. https://doi.org/10.3390/ijms23073448
Oliva MÁ, Tosat-Bitrián C, Barrado-Gil L, Bonato F, Galindo I, Garaigorta U, Álvarez-Bernad B, París-Ogáyar R, Lucena-Agell D, Giménez-Abián JF, et al. Effect of Clinically Used Microtubule Targeting Drugs on Viral Infection and Transport Function. International Journal of Molecular Sciences. 2022; 23(7):3448. https://doi.org/10.3390/ijms23073448
Chicago/Turabian StyleOliva, María Ángela, Carlota Tosat-Bitrián, Lucía Barrado-Gil, Francesca Bonato, Inmaculada Galindo, Urtzi Garaigorta, Beatriz Álvarez-Bernad, Rebeca París-Ogáyar, Daniel Lucena-Agell, Juan Francisco Giménez-Abián, and et al. 2022. "Effect of Clinically Used Microtubule Targeting Drugs on Viral Infection and Transport Function" International Journal of Molecular Sciences 23, no. 7: 3448. https://doi.org/10.3390/ijms23073448