Immunization with Genetically Modified Trypanosomes Provides Protection against Transmissible Spongiform Encephalopathies
Abstract
:1. Introduction
2. Results
2.1. Generation of Engineered T. brucei with Prion Peptide Expressing Surface VSG
2.2. Immunization with the Trypanosomes Leads to the Production of Anti-PrP Antibodies
2.3. Immunization Provides Partial Protection in a Murine Model of Prion Diseases
3. Discussion
4. Materials and Methods
4.1. Generation of Expression Plasmids
4.2. Trypanosoma Brucei Growth and Transfection
4.3. Flow Cytometry
4.4. Immunization and Bioassay
4.5. Western Blotting
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Triller, G.; Garyfallos, D.A.; Papavasiliou, F.N.; Sklaviadis, T.; Stavropoulos, P.; Xanthopoulos, K. Immunization with Genetically Modified Trypanosomes Provides Protection against Transmissible Spongiform Encephalopathies. Int. J. Mol. Sci. 2022, 23, 10629. https://doi.org/10.3390/ijms231810629
Triller G, Garyfallos DA, Papavasiliou FN, Sklaviadis T, Stavropoulos P, Xanthopoulos K. Immunization with Genetically Modified Trypanosomes Provides Protection against Transmissible Spongiform Encephalopathies. International Journal of Molecular Sciences. 2022; 23(18):10629. https://doi.org/10.3390/ijms231810629
Chicago/Turabian StyleTriller, Gianna, Dimitrios A. Garyfallos, F. Nina Papavasiliou, Theodoros Sklaviadis, Pete Stavropoulos, and Konstantinos Xanthopoulos. 2022. "Immunization with Genetically Modified Trypanosomes Provides Protection against Transmissible Spongiform Encephalopathies" International Journal of Molecular Sciences 23, no. 18: 10629. https://doi.org/10.3390/ijms231810629