Systemic Bile Acids Affect the Severity of Acute Pancreatitis in Mice Depending on Their Hydrophobicity and the Disease Pathogenesis
Abstract
:1. Introduction
2. Results
2.1. Impact of Hydrophobic and Hydrophilic BAs in Mouse Isolated Acini
2.2. BAs Were Elevated in the Serum and Reached the Pancreas after Intravenous or Intraperitoneal Administration in Mice
2.3. Hydrophobicity-Dependent Effects of BAs in CCK-Dependent Mouse AP Models
2.4. Aggravating Effects of Hydrophobic BAs in CCK-Independent AP Models in Mice
2.5. Interaction of Hydrophobic and Hydrophilic BAs with CCK1R on Mouse Isolated Acini
3. Discussion
4. Materials and Methods
4.1. Chemicals and Materials
4.2. Isolation and Stimulation of Mouse Pancreatic Acini
4.3. CCK1 Receptor-Binding Assay
4.4. Protease Activation Assays
4.5. Quantification of Intracellular Calcium Mobilization in Living Acinar Cells
4.6. Amylase and Lipase Measurement
4.7. Measurement of Propidium Iodide Exclusion and Release of LDH
4.8. Myeloperoxidase Measurement
4.9. Measurement of Total Bile Acid
4.10. Histopathological Examinations
4.11. Animal Models
4.12. Statistics
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Acknowledgments
Conflicts of Interest
References
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Tran, Q.T.; Sendler, M.; Wiese, M.L.; Doller, J.; Zierke, L.; Gischke, M.; Glaubitz, J.; Tran, V.H.; Lalk, M.; Bornscheuer, U.T.; et al. Systemic Bile Acids Affect the Severity of Acute Pancreatitis in Mice Depending on Their Hydrophobicity and the Disease Pathogenesis. Int. J. Mol. Sci. 2022, 23, 13592. https://doi.org/10.3390/ijms232113592
Tran QT, Sendler M, Wiese ML, Doller J, Zierke L, Gischke M, Glaubitz J, Tran VH, Lalk M, Bornscheuer UT, et al. Systemic Bile Acids Affect the Severity of Acute Pancreatitis in Mice Depending on Their Hydrophobicity and the Disease Pathogenesis. International Journal of Molecular Sciences. 2022; 23(21):13592. https://doi.org/10.3390/ijms232113592
Chicago/Turabian StyleTran, Quang Trung, Matthias Sendler, Mats L. Wiese, Julia Doller, Lukas Zierke, Marcel Gischke, Juliane Glaubitz, Van Huy Tran, Michael Lalk, Uwe T. Bornscheuer, and et al. 2022. "Systemic Bile Acids Affect the Severity of Acute Pancreatitis in Mice Depending on Their Hydrophobicity and the Disease Pathogenesis" International Journal of Molecular Sciences 23, no. 21: 13592. https://doi.org/10.3390/ijms232113592