Transcriptomics and Phenotypic Analysis of gpr56 Knockout in Zebrafish
Abstract
:1. Introduction
2. Results
2.1. Construction of gpr56-Knockout Zebrafish Lines
2.2. Analysis of Transcriptome Sequencing Data
2.3. Deletion of gpr56 Leads to an Imbalance of Innate Immunity-Related Gene Expression in Zebrafish Juveniles
2.4. The Absence of gpr56 Causes Multiple Damages to Pancreatic Exocrine Secretion
2.5. Deletion of gpr56 Makes 5 dpf Zebrafish More Active
3. Discussion
4. Materials and Methods
4.1. Experimental Zebrafish and Gene Knockout
4.2. RNA-seq Transcriptome Analysis
4.3. qPCR Detection
4.4. Synthesis of RNA Probes and Whole In Situ Hybridization (WISH)
4.5. Imaging and Image Analysis
4.6. Motor Ability Test
4.7. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Sun, L.; Yang, B.; Peng, Z.; Yang, T.; Qin, B.; Ao, J.; Yang, Y.; Wang, J.; Zheng, L.; Xie, H. Transcriptomics and Phenotypic Analysis of gpr56 Knockout in Zebrafish. Int. J. Mol. Sci. 2023, 24, 7740. https://doi.org/10.3390/ijms24097740
Sun L, Yang B, Peng Z, Yang T, Qin B, Ao J, Yang Y, Wang J, Zheng L, Xie H. Transcriptomics and Phenotypic Analysis of gpr56 Knockout in Zebrafish. International Journal of Molecular Sciences. 2023; 24(9):7740. https://doi.org/10.3390/ijms24097740
Chicago/Turabian StyleSun, Luning, Boyu Yang, Zheng Peng, Tianle Yang, Bin Qin, Jieyu Ao, Yanqun Yang, Jingling Wang, Lan Zheng, and Huaping Xie. 2023. "Transcriptomics and Phenotypic Analysis of gpr56 Knockout in Zebrafish" International Journal of Molecular Sciences 24, no. 9: 7740. https://doi.org/10.3390/ijms24097740