Delineating Zinc Influx Mechanisms during Platelet Activation
Abstract
:1. Introduction
2. Results
2.1. TRP Channels and Reverse-Mode NCX Regulate Zn2+ Influx during Zn2+-Induced Platelet Activation
2.2. Zn2+-Induced Platelet Activation Is Associated with the Phosphorylation of Substrates of Ca2+-Dependent Enzymes
2.3. Zn2+ Influx Is Mediated by Ca2+ Store Depletion
2.4. [Zn2+]o Regulates Intracellular Signaling via the Regulation of Zn2+ Stores
3. Discussion
4. Materials and Methods
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Drug | Target | IC50 |
---|---|---|
2-APB | TRP channels/SOCE | 34.5 ± 3.0 µM |
FFA | TRP channels | 76.7 ± 9.8 µM |
KB-R | Reverse-mode NCX | 28.9 ± 1.3 µM |
SKF | TRP channels | 23.9 ± 2.5 µM |
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Kuravi, S.J.; Ahmed, N.S.; Taylor, K.A.; Capes, E.M.; Bye, A.; Unsworth, A.J.; Gibbins, J.M.; Pugh, N. Delineating Zinc Influx Mechanisms during Platelet Activation. Int. J. Mol. Sci. 2023, 24, 11689. https://doi.org/10.3390/ijms241411689
Kuravi SJ, Ahmed NS, Taylor KA, Capes EM, Bye A, Unsworth AJ, Gibbins JM, Pugh N. Delineating Zinc Influx Mechanisms during Platelet Activation. International Journal of Molecular Sciences. 2023; 24(14):11689. https://doi.org/10.3390/ijms241411689
Chicago/Turabian StyleKuravi, Sahithi J., Niaz S. Ahmed, Kirk A. Taylor, Emily M. Capes, Alex Bye, Amanda J. Unsworth, Jonathan M. Gibbins, and Nicholas Pugh. 2023. "Delineating Zinc Influx Mechanisms during Platelet Activation" International Journal of Molecular Sciences 24, no. 14: 11689. https://doi.org/10.3390/ijms241411689