VDUP1 Deficiency Promotes the Severity of DSS-Induced Colitis in Mice by Inducing Macrophage Infiltration
Abstract
:1. Introduction
2. Results
2.1. The Expression of VDUP1 Was Reduced in Experimental Colitis
2.2. VDUP1 Deficiency Exacerbated the Severity of DSS-Induced Colitis
2.3. VDUP1 Deficiency Accelerated Colonic Tissue Damage in Experimental Colitis
2.4. VDUP1 Deficiency Induced Inflammatory Cytokine Expression in Experimental Colitis
2.5. VDUP1 Deficiency Activated NF-κB p65 in Experimental Colitis
2.6. VDUP1 Deficiency Promoted Macrophage Chemotaxis to the Site of Inflammation
3. Discussion
4. Materials and Methods
4.1. Reagents and Animals
4.2. DSS-Induced Colitis
4.3. RNA Isolation and Quantification of mRNA Expression
4.4. Colon Histology, Immunohistochemistry, Immunofluorescence Analysis, and TUNEL Assay
4.5. AB-PAS Staining
4.6. Primary Cell Culture and Chemotaxis Assay
4.7. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Park, K.H.; Lee, H.; Kim, H.-C.; Choi, I.; Han, S.-B.; Kang, J.S. VDUP1 Deficiency Promotes the Severity of DSS-Induced Colitis in Mice by Inducing Macrophage Infiltration. Int. J. Mol. Sci. 2023, 24, 13584. https://doi.org/10.3390/ijms241713584
Park KH, Lee H, Kim H-C, Choi I, Han S-B, Kang JS. VDUP1 Deficiency Promotes the Severity of DSS-Induced Colitis in Mice by Inducing Macrophage Infiltration. International Journal of Molecular Sciences. 2023; 24(17):13584. https://doi.org/10.3390/ijms241713584
Chicago/Turabian StylePark, Ki Hwan, Hyunju Lee, Hyoung-Chin Kim, Inpyo Choi, Sang-Bae Han, and Jong Soon Kang. 2023. "VDUP1 Deficiency Promotes the Severity of DSS-Induced Colitis in Mice by Inducing Macrophage Infiltration" International Journal of Molecular Sciences 24, no. 17: 13584. https://doi.org/10.3390/ijms241713584