An Insight into Functional Metagenomics: A High-Throughput Approach to Decipher Food–Microbiota–Host Interactions in the Human Gut
Abstract
:1. Metagenomics of the Human Microbiota
2. Functional Metagenomics of Food–Microbiota–Host Interactions
2.1. Functional Metagenomic—The Pipeline Explained
2.2. Functional Metagenomics of Food–Microbiota Interactions
2.3. Functional Metagenomics of Microbiota–Host Interactions
2.4. Hot-Spots in Functional Metagenomics
Reporter Cell Lines | Target | References |
---|---|---|
Caco-2-AhR-Luc | AhR | [55] |
Colo-205-IL-10-Luc | IL-10 | [59] |
HCT116-HSF1-GFP | Heat shock factor 1 | [60] |
HEK293:NF-κB:GFP | NF-kB | [47,48] |
Hela-HSF1-GFP | Heat shock factor 1 | [60] |
Hela-LC3-GFP | Autophagosome marker LC3 | [61] |
HT-29- TGFβ1-Luc | TGFβ1 | [56] |
HT-29-AhR-Luc | AhR | [55] |
HT-29-AP1-Luc | AP1 | [62] |
HT-29-CCND1-Luc | Cyclin D1 | [63] |
HT-29-HSF1-GFP | Heat shock factor 1 | [60] |
HT-29-IDO-1-Luc | IDO-1 | [57] |
HT-29-Muc2-LUC | Muc2 | [64] |
HT-29-NF-kB-SEAP | NF-kB | [46] |
HT-29-PPARγ-Luc | PPARγ | [42] |
HT-29-TSLP-Luc | TSLP | [65] |
SW1116-ANGPTL4-luc | AngPTL4 | [58] |
3. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Mathieu, E.; Léjard, V.; Ezzine, C.; Govindin, P.; Morat, A.; Giat, M.; Lapaque, N.; Doré, J.; Blottière, H.M. An Insight into Functional Metagenomics: A High-Throughput Approach to Decipher Food–Microbiota–Host Interactions in the Human Gut. Int. J. Mol. Sci. 2023, 24, 17630. https://doi.org/10.3390/ijms242417630
Mathieu E, Léjard V, Ezzine C, Govindin P, Morat A, Giat M, Lapaque N, Doré J, Blottière HM. An Insight into Functional Metagenomics: A High-Throughput Approach to Decipher Food–Microbiota–Host Interactions in the Human Gut. International Journal of Molecular Sciences. 2023; 24(24):17630. https://doi.org/10.3390/ijms242417630
Chicago/Turabian StyleMathieu, Elliot, Véronique Léjard, Chaima Ezzine, Pauline Govindin, Aurélien Morat, Margot Giat, Nicolas Lapaque, Joël Doré, and Hervé M. Blottière. 2023. "An Insight into Functional Metagenomics: A High-Throughput Approach to Decipher Food–Microbiota–Host Interactions in the Human Gut" International Journal of Molecular Sciences 24, no. 24: 17630. https://doi.org/10.3390/ijms242417630