Mass Spectrometry-Based Metabolomic and Lipidomic Analysis of the Effect of High Fat/High Sugar Diet and GreenshellTM Mussel Feeding on Plasma of Ovariectomized Rats
Abstract
:1. Introduction
2. Results
Metabolomic Profile Change in Rats Fed with HFHS Diet and GSM
3. Discussion
4. Methods
4.1. Experimental Methods
4.2. Metabolomic Analysis
4.2.1. Chemicals and Reagents
4.2.2. Sample Preparation
4.2.3. Instruments and Conditions
4.2.4. Data Processing and Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Lipids | Average Rt (Min) | Adduct Type | Fold Change | ||
---|---|---|---|---|---|
HFHS/ Control | HFHS + GSM/HFHS | Control + GSM/ Control | |||
DG 38:5 | 8.227 | [M+Na]+ | 0.54 * | 0.76 | 0.49 * |
DG 38:6 | 7.444 | [M+Na]+ | 0.76 | 0.83 | 0.61 * |
DG 47:10 | 6.276 | [M+Na]+ | 0.74 * | 1.09 | 1.2 * |
DG 47:9 | 6.188 | [M+Na]+ | 0.56 * | 0.78 | 0.66 * |
DG 49:10 | 7.184 | [M+Na]+ | 0.89 | 1.12 | 1.34 * |
DG 49:12 | 6.177 | [M+Na]+ | 0.69 * | 0.88 | 0.76 * |
LPC 16:1 | 2.228 | [M+H]+ | 0.33 * | 0.96 | 1.19 * |
LPC 20:5 | 1.994 | [M+H]+ | 0.28 * | 2.6 * | 4.38 * |
PC 32:1 | 6.065 | [M+H]+ | 0.19 * | 0.79 | 0.87 |
PC 32:2 | 5.506 | [M+H]+ | 0.46 * | 1.00 | 1.14 |
PC 34:3 | 5.647 | [M+H]+ | 0.42 * | 1.01 | 1.34 * |
PC 34:4 | 5.442 | [M+H]+ | 0.37 * | 0.79 | 0.69 * |
PC 36:5 | 6.268 | [M+H]+ | 0.73 * | 1.1 | 1.19 |
PC 36:6 | 5.639 | [M+H]+ | 0.31 * | 1.24 | 1.32 * |
PC 37:4 | 6.613 | [M+H]+ | 0.65 | 0.74 | 0.6 * |
PC 38:5 | 6.264 | [M+H]+ | 0.41 * | 0.81 | 0.69 |
PC 38:7 | 6.181 | [M+H]+ | 0.7 * | 0.87 | 0.76 * |
PC 38:8 | 5.685 | [M+H]+ | 0.24 * | 1.29 | 2.26 * |
PC 40:4 | 7.786 | [M+H]+ | 0.92 | 0.54 * | 0.32 * |
PC O-36:4 | 6.702 | [M+H]+ | 0.66 * | 0.8 | 0.59 * |
PC O-36:5 | 6.567 | [M+H]+ | 0.69 | 0.83 | 0.59 * |
PC O-39:10 | 6.912 | [M+H]+ | 0.24 * | 0.76 | 0.87 |
PC O-39:4 | 7.076 | [M+H]+ | 0.97 | 0.81 | 0.62 * |
TG 48:1 | 10.944 | [M+NH4]+ | 0.2 * | 0.97 | 0.84 |
TG 50:3 | 10.844 | [M+NH4]+ | 0.25 * | 1.08 | 1.03 |
TG 54:7 | 10.732 | [M+NH4]+ | 0.62 * | 1.4 * | 1.88 * |
TG 54:8 | 10.638 | [M+NH4]+ | 0.66 * | 1.36 | 2.03 * |
TG 56:10 | 10.589 | [M+NH4]+ | 1.07 | 1.36 | 2.22 * |
TG 60:12 | 10.677 | [M+NH4]+ | 1.46 * | 1.16 | 1.94 * |
Fold Change | |||||
---|---|---|---|---|---|
Lipids | Average Rt (min) | Adduct Type | HFHS/ Control | HFHS + GSM/HFHS | Control + GSM/ Control |
Cer 41:1 | 10.315 | [M+H-H2O]+ | 0.54 * | 0.63 | 0.69 * |
DG 38:5 | 8.227 | [M+Na]+ | 0.62 * | 0.69 * | 0.54 * |
DG 38:6 | 7.444 | [M+Na]+ | 0.91 | 0.74 * | 0.61 * |
DG 47:9 | 6.188 | [M+Na]+ | 0.47 * | 0.77 | 0.71 * |
DG 49:10 | 7.184 | [M+Na]+ | 0.91 | 1.02 | 1.2 * |
DG 49:12 | 6.177 | [M+Na]+ | 0.65 * | 0.8 | 0.78 * |
LPC 20:5 | 1.994 | [M+H]+ | 0.41 * | 1.58 * | 3.14 * |
PC 32:1 | 6.065 | [M+H]+ | 0.21 * | 0.97 | 0.89 |
PC 32:2 | 5.506 | [M+H]+ | 0.4 * | 1.18 | 1.05 |
PC 33:2 | 5.87 | [M+H]+ | 0.47 * | 0.88 | 1.27 |
PC 34:3 | 5.647 | [M+H]+ | 0.44 * | 1.01 | 1.19 |
PC 34:4 | 5.442 | [M+H]+ | 0.27 * | 0.74 | 0.68 * |
PC 35:2 | 6.695 | [M+H]+ | 0.68 * | 1.03 | 1.34 * |
PC 36:4 | 6.186 | [M+H]+ | 0.57 * | 0.79 | 0.78 * |
PC 36:6 | 5.639 | [M+H]+ | 0.28 * | 1.06 | 1.08 |
PC 37:4 | 6.613 | [M+H]+ | 0.49 * | 0.83 | 0.8 |
PC 38:5 | 6.264 | [M+H]+ | 0.38 * | 0.86 | 0.77 |
PC 38:7 | 6.181 | [M+H]+ | 0.65 * | 0.84 | 0.78 * |
PC 38:8 | 5.685 | [M+H]+ | 0.26 * | 1.43 | 2.16 * |
PC 40:4 | 7.786 | [M+H]+ | 0.71 | 0.63 * | 0.32 * |
PC O-36:4 | 6.702 | [M+H]+ | 0.5 * | 0.82 | 0.62 * |
PC O-36:5 | 6.567 | [M+H]+ | 0.49 * | 0.84 | 0.63 * |
PC O-39:4 | 7.076 | [M+H]+ | 0.75 * | 0.82 | 0.7 * |
PC O-40:8 | 6.79 | [M+H]+ | 0.71 * | 0.77 * | 0.63 * |
TG 49:2 | 10.894 | [M+NH4]+ | 0.52 * | 1.4 | 1.35 * |
TG 51:3 | 10.905 | [M+NH4]+ | 0.96 | 1.05 | 1.47 * |
TG 54:7 | 10.732 | [M+NH4]+ | 1.1 | 1.11 | 2 * |
TG 54:8 | 10.638 | [M+NH4]+ | 1.12 | 1.04 | 1.8 * |
TG 56:10 | 10.589 | [M+NH4]+ | 1.09 | 1.3 | 1.87 * |
Metabolites | Average Rt(Min) | Adduct Type | Fold Change | ||
---|---|---|---|---|---|
HFHS/ Control | HFHS + GSM/ HFHS | Control + GSM/ Control | |||
Cholic acid | 2.572 | [M+H]+ | 1.42 * | 1.02 | 0.59 |
Serine | 11.141 | [M+H]+ | 1.28 | 1.06 | 0.65 * |
Threonine | 10.263 | [M+H]+ | 0.94 | 1.21 | 0.58 * |
Lysine | 9.533 | [M+H]+ | 1.37 * | 0.95 | 0.90 * |
Valine | 6.481 | [M+H]+ | 1.19 | 0.82 * | 0.71 * |
Pipecolic acid | 9.534 | [M+H]+ | 1.69 * | 0.91 | 0.33 * |
Histidine | 8.167 | [M-H]− | 0.97 | 0.87 * | 0.93 |
3-Methylcytidine | 6.516 | [M+H]+ | 0.83 | 0.99 | 1.45 * |
Metabolites | Average Rt (Min) | Adduct Type | Fold Change | ||
---|---|---|---|---|---|
HFHS/ Control | HFHS + GSM/ HFHS | Control + GSM/ Control | |||
3- hydroxybutyric acid | 6.863 | [M-H]− | 0.55 * | 0.98 | 1.16 |
Valine | 6.481 | [M+H]+ | 1.03 | 0.80 | 0.63 * |
Cholic acid | 2.572 | [M+H]+ | 1.38 * | 0.42 | 1.23 |
Tryptophan | 7.968 | [M+H]+ | 0.80 * | 1.06 | 0.91 |
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Abshirini, M.; Cabrera, D.; Fraser, K.; Siriarchavatana, P.; Wolber, F.M.; Miller, M.R.; Tian, H.S.; Kruger, M.C. Mass Spectrometry-Based Metabolomic and Lipidomic Analysis of the Effect of High Fat/High Sugar Diet and GreenshellTM Mussel Feeding on Plasma of Ovariectomized Rats. Metabolites 2021, 11, 754. https://doi.org/10.3390/metabo11110754
Abshirini M, Cabrera D, Fraser K, Siriarchavatana P, Wolber FM, Miller MR, Tian HS, Kruger MC. Mass Spectrometry-Based Metabolomic and Lipidomic Analysis of the Effect of High Fat/High Sugar Diet and GreenshellTM Mussel Feeding on Plasma of Ovariectomized Rats. Metabolites. 2021; 11(11):754. https://doi.org/10.3390/metabo11110754
Chicago/Turabian StyleAbshirini, Maryam, Diana Cabrera, Karl Fraser, Parkpoom Siriarchavatana, Frances M. Wolber, Matthew R. Miller, Hong Sabrina Tian, and Marlena C. Kruger. 2021. "Mass Spectrometry-Based Metabolomic and Lipidomic Analysis of the Effect of High Fat/High Sugar Diet and GreenshellTM Mussel Feeding on Plasma of Ovariectomized Rats" Metabolites 11, no. 11: 754. https://doi.org/10.3390/metabo11110754