A Differential Proteomic Approach to Characterize the Cell Wall Adaptive Response to CO2 Overpressure during Sparkling Wine-Making Process
Abstract
:1. Introduction
2. Materials and Methods
2.1. Yeast Strains and Conditions
2.2. Proteomic Analysis
2.3. Confidence Parameters and Statistics
3. Results and Discussion
3.1. Glucan Processing and Remodeling
3.2. Glucan and Chitin Assembly
3.3. Mannoproteins
3.4. Cell Separation
3.5. Proteins Related to Flocculation, Cell Adhesion, and Biofilm Formation
3.6. Other Cell Wall Proteins
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Yeast Strains | S. cerevisiae P29 | S. cerevisiae G1 | ||||||
---|---|---|---|---|---|---|---|---|
Conditions | T1 | Funcion | T2 | Function | T1 | Function | T2 | Function |
Protein | Ecm33p (2.1) | GPI-anchored protein | - | - | Cis3p (1.8) | Mannoprotein | Hsp150p (1.8) | O-mannosylated heat shock protein |
Gas1p (1.8) | β-1,3-glucanosyltransferase | - | - | Exg2p (2.4) | β-glucan assembly | Pst1p (1.8) | Cell wall protein with GPI (Glycosylphosphatidylinositol)-attachment site | |
Pst1p (2.3) | Cell wall protein with GPI-attachment site | - | - | Pst1p (1.9) | Cell wall protein with GPI-attachment site | Scw4p (3) | Cell wall protein with similarity to glucanases | |
Ssa1p (2) | Protein folding | - | - | Ssa1p (2.1) | Protein folding | - | - | |
Ssa2p (1.9) | Protein folding | - | - | Ssa2p (2.1) | Protein folding | - | - |
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Porras-Agüera, J.A.; Mauricio, J.C.; Moreno-García, J.; Moreno, J.; García-Martínez, T. A Differential Proteomic Approach to Characterize the Cell Wall Adaptive Response to CO2 Overpressure during Sparkling Wine-Making Process. Microorganisms 2020, 8, 1188. https://doi.org/10.3390/microorganisms8081188
Porras-Agüera JA, Mauricio JC, Moreno-García J, Moreno J, García-Martínez T. A Differential Proteomic Approach to Characterize the Cell Wall Adaptive Response to CO2 Overpressure during Sparkling Wine-Making Process. Microorganisms. 2020; 8(8):1188. https://doi.org/10.3390/microorganisms8081188
Chicago/Turabian StylePorras-Agüera, Juan Antonio, Juan Carlos Mauricio, Jaime Moreno-García, Juan Moreno, and Teresa García-Martínez. 2020. "A Differential Proteomic Approach to Characterize the Cell Wall Adaptive Response to CO2 Overpressure during Sparkling Wine-Making Process" Microorganisms 8, no. 8: 1188. https://doi.org/10.3390/microorganisms8081188