Search Results (38)

This study investigates the flexural performance of desert sand concrete-filled steel tube (DS-CFST) members through experimental validation and finite element modeling (FEM). An extensive database of square and circular CFST specimens subjected to pure bending was analyzed to validate an ABAQUS-based FEM. Parametric studies evaluated the influence of steel yield strength, steel ratio, stirrup confinement, and desert sand replacement ratio (r) on ultimate bending moment, stiffness, and failure modes. The results indicated that steel yield strength and section geometry significantly affected bending capacity, while desert sand substitution (r ≤ 1) had a negligible impact on capacity, reducing it by less than 3%. The FEM accurately predicted buckling patterns, moment-curvature relationships, and failure modes. New design formulas for predicting ultimate bending moment and flexural stiffness were proposed, demonstrating superior accuracy (mean error < 1%) compared to existing design codes (AIJ, AISC, GB). This study highlights that DS-CFST members, particularly circular sections, offer robust flexural performance, with enhanced ductility and uniform stress distribution. The findings underscore the potential of using desert sand as a sustainable material in concrete-filled steel tube structures without compromising structural integrity. Full article

The global growth of solar power has led to a significant increase in solar photovoltaics (PV) waste, which is expected to rise significantly in the coming years. The recommended end-of-life (EOL) management techniques for wasted PV panels include landfill disposal, recycling, or panel reuse. However, a comprehensive decision-making strategy is necessary to assess the appropriate EOL plans from various perspectives, including economic, environmental, sociological, technological, regulatory, and business. This study aims to establish a comprehensive approach for examining disposition alternatives and suggest guidelines for PV EOL management. The Analytic Hierarchy Process (AHP) is used to prioritize disposition alternatives for solar PV waste, considering five key criteria: environmental impact, economic viability, social implications, policy and legislative compliance, and technical feasibility. The AHP Aggregating Individual Priorities (AIP) aggregation approach is used to analyze data using a pairwise comparisons matrix. The research indicates that recycling is the most preferred option based on the primary criteria, achieving the highest overall score compared to other alternatives. However, discrepancies were observed in the decisions among individual stakeholder groups and subfactor evaluations. To address these variations, this study provides policy recommendations to guide the sector in adopting optimal decision-making strategies for PV EOL management. Full article

Background and Objectives: Pediatric traumatic brain injury (pTBI) remains a major pediatric public health problem, despite well-developed injury prevention programs. The purpose of this study is to analyze the emergency surgical outcomes of pTBI in a single institute ten-year retrospective study to offer a real-world clinical result. Materials and Methods: Our institute presented a clinical retrospective, single-institute research study of 150 pediatric TBI cases that were diagnosed and underwent emergency surgical treatment from 2010 to 2019. Results: The incidence of radiological findings is detailed as follows: brain edema (30%, 45/150), followed by acute subdural hematoma (27.3%, 41/150), epidural hematoma (21.3%, 32/150), chronic subdural hemorrhage (10%, 15/150), skull fracture (6.7%, 10/150), and traumatic subarachnoid hemorrhage (4.7%, 7/150). Surgical intervention data revealed that decompressive craniectomy was still the main effective surgical method. The results showed longer hospital stays and higher morbidity rates in the brain edema, acute subdural hematoma, and chronic subdural hemorrhage groups, which were viewed as poor surgical outcome groups. Epidural hematoma, skull fracture and traumatic subarachnoid hemorrhage were categorized into good surgical outcome groups. Notably, the data revealed gross improvement in Glasgow Coma Scale/Score (GCS) evolution after surgical interventions, and the time to cranioplasty was a significant factor in the development of post-traumatic hydrocephalus (PTH). Conclusions: Our study provided real-world data for the distribution of etiology in pTBI and also categorized it into six groups, indicating disease-orientated treatment. In addition, our data supported that decompressive craniectomy (DC) remains a mainstay surgical treatment in pTBI and early cranioplasty could decrease the incidence of PTH. Full article

Glioblastoma multiforme (GBM) is a highly aggressive brain cancer with a poor prognosis despite current treatments. This is partially attributed to the immunosuppressive environment facilitated by tumor-associated macrophages, which predominantly underlie the tumor-promoting M2 phenotype. This study investigated the potential of hyperbaric oxygen (HBO) therapy, traditionally used to treat conditions such as decompression sickness, in modulating the macrophage phenotype toward the tumoricidal M1 state and disrupting the supportive tumor microenvironment. HBO has direct antiproliferative effects on tumor cells and reduces hypoxia, which may impair angiogenesis and tumor growth. This offers a novel approach to GBM treatment by targeting the role of the immune system within the tumor microenvironment. The effects of HBO on macrophage polarization and GBM cell viability and apoptosis were evaluated in this study. We detected that HBO promoted M1 macrophage cytokine expression while decreasing GBM cell viability and increasing apoptosis using GBM cell lines and THP-1-derived macrophage-conditioned media. These findings suggest that HBO therapy can shift macrophage polarization toward a tumoricidal M1 state. This can improve GBM cell survival and offers a potential therapeutic strategy. In conclusion, HBO can shift macrophages from a tumor-promoting M2 phenotype to a tumoricidal M1 phenotype in GBM. This can facilitate apoptosis and, in turn, improve treatment outcomes. Full article

Gliomas are the most common primary brain tumors in adults. Despite multidisciplinary treatment approaches, the survival rates for patients with malignant glioma have only improved marginally, and few prognostic biomarkers have been identified. Peroxisome proliferator-activated receptor γ (PPARγ) coactivator-1α (PGC-1α) is a crucial regulator of cancer metabolism, playing a vital role in cancer cell adaptation to fluctuating energy demands. In this study, the clinicopathological roles of PGC-1α in gliomas were evaluated. Employing immunohistochemistry, cell culture, siRNA transfection, cell viability assays, western blot analyses, and in vitro and in vivo invasion and migration assays, we explored the functions of PGC-1α in glioma progression. High PGC-1α expression was significantly associated with an advanced pathological stage in patients with glioma and with poorer overall survival. The downregulation of PGC-1α inhibited glioma cell proliferation, invasion, and migration and altered the expression of oncogenic markers. These results conclusively demonstrated that PGC-1α plays a critical role in maintaining the malignant phenotype of glioma cells and indicated that targeting PGC-1α could be an effective strategy to curb glioma progression and improve patient survival outcomes. Full article

The geometric shape of the roof and the opening position are important parameters influencing the internal cross-ventilation of buildings. Although there has been extensive research on natural ventilation, most of it has focused on flat or sloping roofs with the same opening positions. There is still limited research on the impact of different opening positions and sloping roofs on natural ventilation. In this study, computational fluid dynamics (CFD) was used to investigate the air exchange efficiency (AEE) in general isolated buildings. These buildings encompassed three distinct opening configurations (top–top, top–bottom, and bottom–top) and six varying slope angles for gable roofs (0°, 9°, 18°, 27°, 36°, and 45°). Computational simulations were carried out using the SST k-omega turbulence model, and validation was performed against experimental data supplied by the Japanese AIJ Wind Tunnel Laboratory. Grid independence validation was also conducted to ensure the reliability of the CFD simulation results. The study revealed that the highest AEE was 48.1%, achieved with the top–bottom opening configuration and a gable roof slope angle of 45°. Conversely, the lowest AEE was 31.4%, attained with the bottom–top opening configuration and a gable roof slope angle of 27°. Furthermore, it was observed that when the opening configuration was set to top–top and bottom–top, the slope angle of the gable roof had minimal influence on AEE, with an average AEE of only around 33%. When the opening configuration was top–bottom, it was found that there was a positive correlation between the gable roof slope angle and AEE. Full article

Glioblastoma multiforme (GBM) is the most common and deadliest primary brain tumor in adults. Despite the advances in GBM treatment, outcomes remain poor, with a 2-year survival rate of less than 5%. Hyperbaric oxygen (HBO) therapy is an intermittent, high-concentration, short-term oxygen therapy used to increase cellular oxygen content. In this study, we evaluated the effects of HBO therapy, alone or combined with other treatment modalities, on GBM in vitro and in vivo. In the in vitro analysis, we used a 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay to assess the effects of HBO therapy alone, a colony formation assay to analyze the effects of HBO therapy combined with radiotherapy and with temozolomide (TMZ), and a neurosphere assay to assess GBM stemness. In the in vivo analysis, we used immunohistochemical staining and in vivo bioluminescence imaging to assess GBM stemness and the therapeutic effect of HBO therapy alone or combined with TMZ or radiotherapy, respectively. HBO therapy did not affect GBM cell viability, but it did reduce the analyzed tumors’ ability to form cancer stem cells. In addition, HBO therapy increased GBM sensitivity to TMZ and radiotherapy both in vitro and in vivo. HBO therapy did not enhance tumor growth and exhibited adjuvant effects to chemotherapy and radiotherapy through inhibiting GBM stemness. In conclusion, HBO therapy shows promise as an adjuvant treatment for GBM by reducing cancer stem cell formation and enhancing sensitivity to chemotherapy and radiotherapy. Full article

Glioblastoma (GBM) stands as the most prevalent primary malignant brain tumor, typically resulting in a median survival period of approximately thirteen to fifteen months after undergoing surgery, chemotherapy, and radiotherapy. Nucleobindin-2 (NUCB2) is a protein involved in appetite regulation and energy homeostasis. In this study, we assessed the impact of NUCB2 expression on tumor progression and prognosis of GBM. We further evaluated the relationship between NUCB2 expression and the sensitivity to chemotherapy and radiotherapy in GBM cells. Additionally, we compared the survival of mice intracranially implanted with GBM cells. High NUCB2 expression was associated with poor prognosis in patients with GBM. Knockdown of NUCB2 reduced cell viability, migration ability, and invasion ability of GBM cells. Overexpression of NUCB2 resulted in reduced apoptosis following temozolomide treatment and increased levels of DNA damage repair proteins after radiotherapy. Furthermore, mice intracranially implanted with NUCB2 knockdown GBM cells exhibited longer survival compared to the control group. NUCB2 may serve as a prognostic biomarker for poor outcomes in patients with GBM. Additionally, NUCB2 not only contributes to tumor progression but also influences the sensitivity of GBM cells to chemotherapy and radiotherapy. Therefore, targeting NUCB2 protein expression may represent a novel therapeutic approach for the treatment of GBM. Full article

Glioblastoma (GBM) is the most common primary brain malignancy in adults. Despite multimodal treatment that involves maximal safe resection, concurrent chemoradiotherapy, and tumour treatment for supratentorial lesions, the prognosis remains poor. The current median overall survival is only <2 years, and the 5-year survival is only 7.2%. Thioredoxin domain-containing protein 11 (TXNDC11), also known as EF-hand binding protein 1, was reported as an endoplasmic reticulum stress-induced protein. The present study aimed to elucidate the prognostic role of TXNDC11 in GBM. We evaluated the clinical parameters and TXNDC11 scores in gliomas from hospitals. Additionally, proliferation, invasion, migration assays, apoptosis, and temozolomide (TMZ)-sensitivity assays of GBM cells were conducted to evaluate the effects of short interfering RNA (siRNA) on these processes. In addition, these cells were subjected to Western blotting to detect the expression levels of N-cadherin, E-cadherin, and Cyclin D1. High levels of TXNDC11 protein expression were significantly associated with World Health Organization (WHO) high-grade tumour classification and poor prognosis. Multivariate analysis revealed that in addition to the WHO grade, TXNDC11 protein expression was also an independent prognostic factor of glioma. In addition, TXNDC11 silencing inhibited proliferation, migration, and invasion and led to apoptosis of GBM cells. However, over-expression of TXNDC11 enhanced proliferation, migration, and invasion. Further, TXNDC11 knockdown downregulated N-cadherin and cyclin D1 expression and upregulated E-cadherin expression in GBM cells. Knock-in TXNDC11 return these. Finally, in vivo, orthotopic xenotransplantation of TXNDC11-silenced GBM cells into nude rats promoted slower tumour growth and prolonged survival time. TXNDC11 is a potential oncogene in GBMs and may be an emerging therapeutic target. Full article

Numerous studies have considered galectin-3 or Glycogen synthase kinase 3 beta (GSK3B) as a potential prognosis marker for various cancers. However, the correlation between the protein expression of galectin-3/GSK3B and the clinical parameters of astrocytoma has not been reported. This study aims to validate the correlation between the clinical outcomes and protein expression of galectin-3/GSK3B in astrocytoma. Immunohistochemistry staining was performed to detect galectin-3/GSK3B protein expression in patients with astrocytoma. The Chi-square test, Kaplan−Meier evaluation, and Cox regression analysis were used to determine the correlation between clinical parameters and galectin-3/GSK3B expression. Cell proliferation, invasion, and migration were compared between a non-siRNA group and a galectin-3/GSK3B siRNA group. Protein expression in galectin-3 or GSK3B siRNA-treated cells was evaluated using western blotting. Galectin-3 and GSK3B protein expression were significantly positively correlated with the World Health Organization (WHO) astrocytoma grade and overall survival time. Multivariate analysis revealed that WHO grade, galectin-3 expression, and GSK3B expression were independent prognostic factors for astrocytoma. Galectin-3 or GSK3B downregulation induced apoptosis and decreased cell numbers, migration, and invasion. siRNA-mediated gene silencing of galectin-3 resulted in the downregulation of Ki-67, cyclin D1, VEGF, GSK3B, p-GSK3B Ser9 (p-GSK3B S9), and β-catenin. In contrast, GSK3B knockdown only decreased Ki-67, VEGF, p-GSK3B S9, and β-catenin protein expression but did not affect cyclin D1 and galectin-3 protein expression. The siRNA results indicated that GSK3B is downstream of the galectin-3 gene. These data support that galectin-3 mediated tumor progression by upregulating GSK3B and β-catenin protein expression in glioblastoma. Therefore, galectin-3 and GSK3B are potential prognostic markers, and their genes may be considered to be anticancer targets for astrocytoma therapy. Full article

PBL stiffeners, made of thin-walled steel plates with circular openings and welded to the steel tube of a square concrete-filled steel tubular (CFST) column, can improve the combined effect effectively by co-carrying axial compressive forces and confining the concrete core. A numerical simulation study based on the previous test was conducted to study the ultimate strength of the CFST stub column with PBL stiffeners. Finite element models of CFST with different stiffeners were made and verified by the test results of typical failure modes and load–strain curves of specimens. The parameter study was conducted, including PBL stiffener detailing (i.e., material strength, stiffener thickness, opening diameter, and opening spacing). Finally, based on the study and analysis results, an ultimate bearing capacity prediction formula was proposed, which can reasonably predict the bearing capacity of a square CFST column with longitudinal or diagonal stiffeners, while the methods in ACI, BS5400, EC4, AIJ, and DBJ were more conservative. Full article

The thin-wall high-strength (HS) steel tube can provide strong confinement ability for core concrete due to its high yield strength, so it can achieve the purpose of confining concrete lateral deformation. In order to make full use of the mechanical properties of HS steel tube and concrete, it is necessary to study the axial compressive properties of the concrete-filled HS steel tube (CFHST) with a large diameter-to-thickness ratio (D/t). In this study, the axial compressive capacity of 15 CFHST columns with a large diameter-to-thickness ratio was tested. Then, a series of finite element (FE) models were developed to study the interaction mechanism between steel tube and concrete, the load–strain curves of typical specimens, and the effect of the key parameters. Finally, a new design proposal for CFHST compressive capacity is proposed and compared with EC4, GB50936-2014, and AIJ-97 prediction results. The results showed that the CFHST has good compressive capacity and can effectively confine the lateral deformation of core concrete; the contact pressure appears a stable stage because with a steel tube is difficult to effectively restrain the lateral deformation after steel tube yielding and partial failure of concrete; the existing design code is conservative in predicting the axial compression capacity of the CFHST with large D/t ratio. Full article

When developing methods for diagnosing pathologies and diseases in humans and animals using electronic noses, one of the important trends is the miniaturization of devices, while maintaining significant information for diagnostic purposes. A combination of several sorbents that have unique sorption features of volatile organic compounds (VOCs) on one transducer is a possible option for the miniaturization of sensors for gas analysis. This paper considers the principles of creating polycomposite coatings on the electrodes of piezoelectric quartz resonators, including the choice of sorbents for the formation of sensitive layers, determining the mass and geometry of the formation of sensitive layers in a polycomposite coating, as well as an algorithm for processing the output data of sensors to obtain maximum information about the qualitative and quantitative composition of the gas phase. A comparative analysis of the efficiency and kinetics of VOC vapor sorption by sensors with polycomposite coatings and a set of sensors with relevant single coatings has been carried out. Regression equations have been obtained to predict the molar-specific sensitivity of the microbalance of VOC vapors by a sensor with a polycomposite coating of three sorbents with an error of 5–15% based on the results of the microbalance of VOC vapors on single coatings. A method for creating “visual prints” of sensor signals with polycomposite coatings is shown, with results comparable to those from an array of sensors. The parameters A_ij∑ are proposed for obtaining information on the qualitative composition of the gas phase when processing the output data of sensors with polycomposite coatings. A biochemical study of exhaled breath condensate (EBC) samples, a microbiological investigation of calf tracheal washes, and a clinical examination were conducted to assess the presence of bovine respiratory disease (BRD). An analysis of the gas phase over EBC samples with an array of sensors with polycomposite coatings was also carried out. The “visual prints” of the responses of sensors with polycomposite coatings and the results of the identification of VOCs in the gas phase over EBC samples were compared to the results of bacteriological studies of tracheal washes of the studied calves. A connection was found between the parameters A_ij∑ of a group of sensors with polycomposite coatings and the biochemical parameters of biosamples. The adequacy of replacing an array of piezoelectric sensors with single coatings by the sensors with polycomposite coatings is shown. Full article

Concrete-filled steel tube (CFST) columns are used in tall buildings and bridges, and they provide more rigidity and higher bearing capacity, but buckling affects their behavior. There is an exceptional need to study the behavior of these columns under various conditions. The numerical method is beneficial in supplementing the experimental works and is used to explore the effects of various parameters because of the limitations in cost, apparatus, and time of the experimental program. The various parameters, such as the different slenderness ratios, i.e., column-height-to-cross-section-dimension (H/D), different steel-tube-thickness-to-column-dimension (D/t), and different compressive strength of concrete to yield strength of steel tube ratio (fc/fy) under concentric axial loading are considered in this current study. Firstly, a finite element model used the “ANSYS” software program and was constructed to validate the results of the experimental works. The extensive numerical models were carried out to extensively widen the study in this field. The numerical work was conducted on sixty-four specimens. Moreover, the analytical calculations from the different international codes/standards were compared with the numerical results to test their reliability in predicting the ultimate carrying loads. The study provided results that show the improvement effect of CFST columns with the high compressive strength of infilled concrete, while no remarkable enhancement effect with the high yield strength of steel tube was observed. Increasing the columns’ diameter is more effective in enhancing the load capacity (about three times more) than increasing the tube thickness (about 1.3 times). Ring stiffeners for long CFST columns (H/D > 12) do not lead to any enhancement of the column behavior due to yielding occurring firstly at the location of the rings. ECP205-2007 is the most conservative design code in predicting the load capacity of CFST columns, while the AIJ design code is good at predicting the ultimate load failure compared to the other codes/standards. Eurocode 4 provides underestimation values of the load-carrying capacity of CFST columns. Full article

Although the expression of p53 and epidermal growth factor receptor (EGFR) is associated with therapeutic resistance and patient outcomes in many malignancies, the relationship in astrocytomas is unclear. This study aims to correlate p53 and EGFR expression in brain astrocytomas with overall patient survival. Eighty-two patients with astrocytomas were enrolled in the study. Semi-quantitative p53 and EGFR immunohistochemical staining was measured in tumor specimens. The mean follow-up after astrocytoma surgery was 18.46 months. The overall survival rate was 83%. Survival was reduced in EGFR-positive patients compared with survival in EGFR-negative patients (p < 0.05). However, no significant differences in survival were detected between patients with high and low p53 expression. In patients with low p53 expression, positive EGFR staining was associated with significantly worse survival compared with patients with negative EGFR staining (log-rank test: p < 0.001). Survival rates in positive and negative EGFR groups with high p53 protein expression were similar (log-rank test: p = 0.919). The IC50 of an EGFR inhibitor was higher in GBM cells with high p53 protein expression compared with the IC50 in cells with low p53 expression. Combined EGFR and p53 expression may have prognostic significance in astrocytomas. Full article