Search Results (872)

Concrete infrastructure in coastal regions is prone to premature degradation due to crack formation under aggressive environmental exposure. Conventional repair methods remain costly and often ineffective. This study evaluates a biomineral self-healing system incorporating Paenibacillus polymyxa spores and calcium carbonate (CaCO₃) to improve the durability and mechanical performance of medium-strength concrete with a design compressive strength of 21 MPa, intended for vulnerable coastal housing. A full factorial experimental program was conducted using three bacterial concentrations (1.0%, 1.5%, 2.0% of mixing water volume) and three CaCO₃ dosages (3%, 5%, 7% as cement replacement). Specimens were pre-cracked under compressive loading, exposed to a simulated coastal environment, and monitored for 28 days. The optimal formulation (2% bacteria + 5% CaCO₃) yielded an 8.8% increase in compressive strength and a 24% increase in flexural strength compared with the control. Crack width reduction reached up to 0.23 mm (65.7%) under wet curing, with effective sealing observed for cracks ≤ 0.5 mm. Recovered compressive strength after healing reached 17.3 MPa, equivalent to 71% of the design strength. These findings demonstrate the potential of P. polymyxa as a viable non-ureolytic agent for self-healing concrete, offering a simple and scalable strategy to extend service life in resource-limited coastal regions while supporting Sustainable Development Goals 9 and 11. Full article

This study investigates the effects of the co-curing process and nanoparticle reinforcement on the mechanical performance of plain-woven glass fiber-reinforced plastic (GFRP) adhesive joints, aiming to address the limitations of traditional fastening methods and the inherent brittleness of epoxy adhesives. Specifically, spherical silica (SiO₂) and plate-like graphene nanoplatelets (GNPs) were incorporated into the epoxy matrix at varying concentrations (0.25 to 1.0 wt.%) to evaluate the influence of particle geometry on joint integrity. Experimental results demonstrated that the co-curing technique yields superior mechanical properties compared to secondary bonding, exhibiting improvements of 35% in shear strength (from 10.97 MPa to 14.83 MPa) and 12% in flexural strength (from 72.57 MPa to 81.28 MPa) due to enhanced chemical interlocking. Furthermore, the addition of nanoparticles significantly improved joint performance, with the optimal content identified at 0.75 wt.% for both particle types. Notably, GNPs outperformed SiO₂, enhancing shear and flexural strengths compared to the neat co-cured baseline. Ultimately, the 0.75 wt.% GNP-reinforced material exhibited a shear strength of 21.22 MPa and a flexural strength of 104.09 MPa. Morphological analysis revealed that while SiO₂ contributes to reinforcement primarily via crack deflection, the high-aspect-ratio GNPs provide superior energy dissipation through crack bridging and pull-out mechanisms. Consequently, this study suggests that the co-curing process combined with an optimal concentration of GNPs presents a highly effective strategy for maximizing the reliability and structural efficiency of composite joints in weight-critical applications. Full article

This study examines the carbonation behavior and CO₂ storage potential of a Ca-rich alkali-activated binder produced entirely from industrial residues-ladle furnace slag (LFS), coal ash (CA), and cement kiln dust (CKD). The system was designed as a one-part alkali-activated material (AAM), with CKD acting as an internal activator, and subjected to ambient curing, water curing, and accelerated CO₂ curing at ambient pressure. Phase evolution, microstructural development, and pore-structure characteristics were investigated using X-ray diffraction, FTIR spectroscopy, DSC–TG analysis, scanning electron microscopy, and X-ray micro-computed tomography, together with measurements of density, water absorption, and compressive strength. Loss-on-ignition measurements combined with chemical analysis were further used to quantify CO₂ uptake and evaluate the degree of carbonation of the binder system. CO₂ curing fundamentally altered the reaction pathway of the binder, shifting it from hydration-dominated to carbonation-controlled phase evolution, leading to the decomposition of calcium-bearing hydrates and complete carbonation of non-hydraulic γ-belite with the formation of vaterite, aragonite, and calcite. These transformations induced pronounced microstructural densification, reflected in a near-doubling of compressive strength (>48 MPa), increased apparent density, reduced water absorption, and simplified pore-network topology. A preliminary carbon footprint assessment indicates that the production of 1 m³ of the developed LFS–CA–CKD concrete generates about 14.36 kg CO₂-eq, while the carbonation process enables significant CO₂ sequestration, resulting in a net negative carbon balance. The results demonstrate that controlled carbonation is an effective post-treatment strategy for waste-derived alkali-activated binders, enabling simultaneous performance enhancement and permanent CO₂ sequestration. Full article

This study investigates the utilization of Abies alba exudate resin for the development of hybrid resins intended as matrices for composite materials. The novelty of this work lies in demonstrating that physically hybridized, bio-derived resin systems based on Abies alba exudate can exhibit distinct mechanical and dynamic behaviors solely by adjusting the solvent-assisted formulation route, without intentional chemical modification and without spectroscopic evidence of co-network formation within the limits of ATR-FTIR analysis, although limited interfacial interactions cannot be excluded. Two formulation routes were explored: (i) dilution of Abies alba exudate in turpentine derived from pine buds, (ii) dilution in ethanol (96%). The diluted resins were subsequently blended with a commercial epoxy system, which was cured with its amine hardener to form solid matrices in which the Abies alba component was physically incorporated. The resulting hybrid resins were characterized by multiple testing methods and further applied in the fabrication of cotton fiber-reinforced composites. The turpentine-based hybrid resin (HR1) showed a rigid mechanical response, with tensile strengths of approximately 13.2–13.5 MPa, compressive strengths of about 30 MPa, Shore D hardness values of 56–58.5, and a low damping ratio (≈0.026). In contrast, the ethanol-based hybrid resin (HR2) exhibited a highly deformable mechanical response, characterized by low tensile strength (≈0.5 MPa), very high elastic recovery, low hardness (<10 Shore D), and a significantly higher damping ratio (≈0.139). To demonstrate their applicability in composite manufacturing, the HR1 matrix was reinforced with cotton fabric, leading to a substantial improvement in tensile strength (25–26 MPa) and flexural strength (35–36 MPa), together with an increased natural frequency. Water absorption tests revealed limited moisture uptake for the neat hybrid resins (≤0.04 g), while the cotton-reinforced composite exhibited higher but largely reversible water absorption (≈21.5%), associated with the hydrophilic nature of the reinforcement. Full article

Background/Objectives: Eliciting robust immune responses against the hepatitis B virus (HBV) through therapeutic vaccination holds promise for curing chronic hepatitis B. We previously developed the heterologous protein prime/viral vector boost clinical vaccine candidate, TherVacB. Here, we evaluated a replication-competent chimeric vesicular stomatitis virus vector (VSV-GP) as an alternative viral vector boost vaccine. Methods: A recombinant VSV-GP vector co-expressing HBV surface and core antigens (VSV-GP-HBs/c) was generated and characterized for antigen expression. Its immunogenicity, antiviral efficacy, and durability were assessed in HBV-naïve and HBV-carrier mice, using protein primed, viral vector-primed, and multi-viral vector boost regimens. Results: VSV-GP-HBs/c efficiently expressed both HBV antigens in vitro. A single immunization with VSV-GP-HBs/c induced only weak HBV-specific immune responses in vivo. Replacing protein priming with VSV-GP-HBs/c resulted in modest immune activation and limited antiviral effects in HBV-carrier mice. In contrast, substituting the modified vaccinia virus Ankara (MVA)-HBs/c boost in the TherVacB regimen with VSV-GP-HBs/c elicited robust HBV-specific antibody responses and strong CD4 and CD8 T-cell immunity, assessed by intracellular IFN-γ staining after peptide stimulation. This regimen achieved a substantial reduction in serum HBsAg levels, numbers of HBV-positive hepatocytes, and intrahepatic HBV-DNA, with antiviral efficacy comparable to that of the classical TherVacB regimen. Notably, a second viral vector boost did not enhance HBV-specific immunity or antiviral efficacy; instead, it promoted dominant vector-specific CD8 T-cell responses. Long-term analyses performed 10 weeks after the last vaccination further demonstrated that a single protein-prime/VSV-GP-HBs/c boost was sufficient to achieve sustained antiviral control. Conclusions: These findings identify VSV-GP-HBs/c as an effective boost vector for therapeutic hepatitis B vaccination and establish protein priming followed by a single viral vector boost as an optimal strategy for sustained antiviral immunity. Full article

In this work, a bio-based thermal insulation composite is developed and processed with epoxidized cottonseed oil (ECSO) as a renewable binder; performance is then assessed at material and building levels by using a natural fiber. Composite insulators were synthesized by mixing clay, fly ash, perlite, and eggshell powder with ECSO at different concentrations (45–55 wt%) and curing temperatures (165–205 °C). The density, thermal conductivity, compressive and tensile strengths, wear resistance, and water absorption capacity of the obtained composites were investigated in detail in extensive experimental work. The density and thermal conductivity were much dependent on the ECSO content and the curing temperature, unbeknownst to us; they significantly decreased with the increasing ECSO content and curing temperature, due to better binder–filler interaction and increased porosity. Among all the tested samples, the lowest thermal conductivity and density were observed for ECSO36, which suggested the best insulation performance. To validate its real-world usability, the best composite (ECSO36) was also tested by an IES-VE building energy simulation under the climate of Ankara in terms of annual energy consumption and CO₂ emission. The results signify that ECSO36 achieves a similar energy consumption and CO₂ emission performance to traditional insulation materials. In summary, the results of this work illustrate that ECSO-based composites have excellent potential to be a green and low-carbon alternative for sustainable building insulation applications. Full article

Soil stabilization is widely applied in transportation engineering to enhance the mechanical performance and serviceability of road subgrades, particularly in fine-grained soils susceptible to moisture-induced deterioration. Although Portland cement provides rapid strength development and high load-bearing capacity, its high energy consumption and associated CO₂ emissions have encouraged the exploration of lower-impact stabilization alternatives. This study presents a performance-based comparative evaluation of fine-grained soils stabilized with Portland cement and kaolin at dosages of 3%, 5%, and 7% by dry soil mass. The experimental program included soil characterization, Standard Proctor compaction testing, and unconfined compressive strength (UCS) testing conducted at curing ages of 0, 7, 14, 28, 90, and 180 days. Cement-treated soils exhibited faster early-age strength development and higher long-term UCS values, supporting applications requiring early load-bearing capacity. In contrast, kaolin-treated soils showed gradual and stable strength gains primarily associated with densification and particle rearrangement mechanisms. Overall, the results demonstrate that kaolin can serve as a viable low-impact stabilizer for low-volume and secondary road infrastructure. The findings support performance-based and sustainability-oriented material selection strategies for context-sensitive road subgrade design. Full article

Alzheimer’s disease (AD) is a progressive neurodegenerative disorder and the leading cause of dementia, for which there is currently no cure. The causes of AD are still not well understood, although 5% of cases are known to have a genetic origin, associated with pathogenic genetic variants of the APP and PSEN1/2 genes. There is growing evidence that both APP and PSEN1/2 are also essential for proper human brain development and neural/neuronal function. This implies that abnormalities in early brain development could increase neuronal vulnerability to AD later in life. Human cerebral organoids (hCOs), generated from induced pluripotent stem cells (iPSCs) from AD patients, provide an exceptional model for better understanding the cellular and molecular mechanisms involved in human brain development, as well as early neurological alterations in the evolution of AD. This review compiles the main studies in which hCOs are used as a model for studying AD and for the discovery of new biomarkers. We also discuss the advantages and applications of these hCOs for studying the early stages of AD from a neurodevelopmental perspective. Finally, we mention the main current challenges in the use of hCOs for future research into AD. Full article

This study investigates the activation potential of various activators for ferronickel slag (FNS) and the associated phase evolution. First, the existing forms of MgO in FNS were identified by analyzing its phase composition across different particle sizes. Subsequently, FNS was activated using six types of activators—Ca(OH)₂, CaO, NaOH, KOH, Na₂CO₃, and a Ca(OH)₂–gypsum composite—under steam curing at 80 °C for 7 days. The setting time, fluidity, hydration products, and mechanical properties of the activated systems were systematically examined. The results show that finer water-cooled FNS particles contain abundant amorphous phases, including amorphous MgO, which can react with Ca-based activators to form hydrotalcite—a reaction not observed with Na- or K-based activators. Compared with Na- or K-based activators, Ca-containing activators, particularly the Ca(OH)₂–gypsum combination, exhibited superior activation performance. In addition, distinct microstructures were observed: NaOH activation promoted the formation of a yarn ball-like N–A–S–H gel, while KOH activation led to a knotted-fiber-bundle-like K–A–S–H phase, the latter showing potential for enhancing the crack resistance of cement-based materials. These findings provide new insights into the activator-dependent hydration mechanisms of FNS and support its value-added utilization in sustainable construction materials. Full article

Reducing the carbon footprint of cement based materials requires approaches beyond replacing cement alone. Mineral carbonation of aggregates offers a simple route to store carbon dioxide permanently while improving material performance. In this study, four steel slag aggregates were evaluated as sand replacements in mortar after pre carbonation, including basic oxygen furnace slag, blast furnace slag, skim slag, and Rockport slag. The aggregates were treated using moisture assisted carbonation with carbon dioxide and then used in mortar made under the same mix design and curing conditions. Bulk chemistry was determined by X-ray fluorescence, carbon uptake was quantified by thermogravimetric analysis, and performance was evaluated using compressive strength, ultrasonic pulse velocity, chemical soundness, freeze thaw resistance, and scanning electron microscopy. Pre-carbonation stored approximately 14–19 wt% CO₂ relative to the dry mass of the slag aggregates, depending on slag type. Mortars with carbonated basic oxygen furnace slag and carbonated blast furnace slag showed clear strength gains at 28 days, along with higher ultrasonic pulse velocity and improved chemical durability. Rockport slag showed modest improvement, while skim slag showed a reduction in strength after carbonation. Microstructural observations indicate that carbonate precipitation filled pores and densified the aggregate paste interface, which explains the strength and durability improvements in the more responsive slags. These laboratory-scale results show that, under the specific moisture-assisted pre-carbonation conditions investigated, pre-carbonation of slag aggregates can combine permanent CO₂ storage with improved mortar performance. However, the magnitude of these benefits depends strongly on slag chemistry and particle structure, highlighting the need for slag-specific carbonation design and further validation under practical conditions. Full article

The cement industry is a major contributor to global CO₂ emissions, creating a need for monitoring techniques that support carbon capture strategies while assessing material performance. This study investigates the accelerated carbonation curing of cement mortar using linear and nonlinear ultrasonic sensing methods, alongside mechanical and gravimetric measurements. Mortar specimens were carbonated for 1–28 days and evaluated using ultrasonic pulse velocity (UPV), the Sideband Peak Count Index (SPC-I) for nonlinear ultrasonic response, compressive strength testing, and mass-based CO₂ uptake analysis. UPV showed sensitivity primarily to bulk material changes, with comparatively less distinction among the observed responses during carbonation curing. In contrast, the SPC-I captured distinct nonlinear responses associated with matrix evolution. Early-age carbonation (<7 days) produced increased nonlinearity, attributed to shrinkage-induced microcracking, whereas extended curing led to reduced SPC-I values, consistent with carbonation curing age. These trends exhibited an inverse correlation with compressive strength, which increased by up to 38.9% on the 28th day compared to the control specimens. Gravimetric analysis confirmed effective CO₂ sequestration, with average specimen mass gains reaching 2.62%. The findings demonstrate that nonlinear ultrasonic sensing provides a sensitive, nondestructive approach for monitoring carbonation curing and linking acoustic signatures to mechanical performance and carbon uptake in cement-based materials. Full article

The incorporation of slag and calcium carbonate (CaCO₃) as clinker-reducing constituents offers significant potential for lowering CO₂ emissions in cement production; however, their combined influence on age-dependent compressive strength remains complex and highly coupled. In this study, a structured literature-based dataset ($N=75$ mix conditions) was compiled from two independent experimental sources to investigate compressive strength development in slag–CaCO₃ blended cementitious systems. Compressive strength at 3 and 28 days was formulated as a multi-output regression problem to explicitly capture the correlated nature of strength evolution between early-age and later-age curing stages. Dataset-level analysis revealed that CaCO₃ replacement exerts a stronger influence on early-age strength (reductions of approximately 15–25%) than on later-age strength (typically within 5–15%), indicating a transition from clinker-dominated hydration to slag-controlled later-age strength development. Compared with independent single-output models, the proposed multi-output framework improved prediction performance by increasing $R^2$ values by approximately 4–6% and reducing RMSE by up to 15–18%. Feature importance analysis identified slag replacement ratio and CaCO₃ dosage as the dominant predictors, while chemical composition descriptors modulated age-dependent sensitivity. The results demonstrate that compressive strength at different curing ages is governed by coupled yet temporally evolving physicochemical mechanisms. From an engineering perspective, CaCO₃ replacement should be evaluated within an integrated compositional design framework that considers curing-age requirements and slag reactivity. Overall, this study provides a transparent and statistically robust approach for analyzing strength evolution in blended cement systems and highlights the value of multi-output learning for age-dependent performance prediction in sustainable cementitious materials. Full article

Cement is one of the primary construction materials in ground improvement applications that employ the binder stabilization method. Due to the high carbon dioxide emissions in its production, evaluating environmentally friendly alternative binder materials is a popular research topic. Industrial by-products such as fly ash (FA) and ground granulated blast-furnace slag (GGBS) are alternatives to traditional cement, especially in deep soil mixing (DSM) applications, and can enhance sustainability in construction projects. Since these materials are not active when used alone, alkali activation is proposed to modify them as binding agents in ground improvement projects. This study presents the outcomes of a primary laboratory test phase for on-site applications. FA and GGBS precursors supplied by local plants, mixed with soil and activator solutions in applicable ratios, and samples were prepared for laboratory tests. Unconfined compression tests were applied with strain measurements after several curing durations, between 1 and 54 weeks. Average compression strength and modulus of elasticity values were recorded at approximately 12.3 MPa and 11.7 GPa, respectively, in samples with an average dosage. An empirical correlation between the strength and stiffness modulus was found. Strength and stiffness values were comparable to traditional materials, indicating the potential of these industrial by-products when activated under alkali conditions. The carbon footprints of cement and alkali-activated by-products were compared based on calculated CO₂-eq emissions. Full article

Carbon fiber-reinforced resin matrix composites (CFRC) are extensively used in aerospace, automotive manufacturing, and sports equipment. However, the brittle nature of the resin matrix causes CFRC to exhibit severe vibrations and noise under dry friction conditions. Enhancing the intrinsic damping properties of the resin matrix serves as a fundamental and effective strategy to mitigate vibration and noise radiation in composite components. This study systematically investigates high-temperature co-curing damping composites using co-curing technology, aiming to improve the mechanical performance and damping characteristics of traditional fiber-reinforced epoxy resin composites. A novel carbon fiber-reinforced terminal carboxyl nitrile epoxy pre-polymer composite material demonstrates both stable chemical properties and excellent high-temperature resistance. Through formulation adjustments, the curing temperature and time of epoxy resin are matched with those of the terminal carboxyl nitrile epoxy pre-polymer. The performance of epoxy carbon fiber composites was evaluated through tensile tests, flexural tests, impact tests, infrared spectroscopy, thermogravimetric analysis, dynamic mechanical analysis, scanning electron microscopy, and X-ray diffraction. Results show that blending epoxy resin with terminal carboxyl nitrile liquid rubber enhances energy dissipation by increasing intermolecular friction and hydrogen bonding interactions. The damping ratio of epoxy resin-based carbon fiber composites reaches as high as 1.67%. Tensile strength, flexural strength, and impact strength reach 1968 MPa, 1343 MPa, and 127 kJ/m², respectively. The addition of terminal carboxylated nitrile liquid rubber facilitates the formation of continuous friction membranes, enhancing friction stability. Tensile tests demonstrate that carbon fiber composites containing 25% terminal carboxylated nitrile liquid rubber outperforms other formulations. As evidenced by impact tests, the performance of the prepared composites is superior to that of other configurations. Dynamic mechanical analysis indicates that the 25% rubber-containing composites exhibit enhanced damping characteristics and higher loss modulus. Experimental results confirm that this study advances the development of functional composites for vibration reduction and noise control applications. Full article

This study combined experimental analysis with deep learning to investigate the effects of curing age, steel slag content, and gradation composition on the mechanical properties of cement-stabilized steel slag (CSSS). The strength evolution patterns and underlying microscopic mechanisms were systematically elucidated. Experimental results showed that CSSS strength grows nonlinearly with curing age, with optimal mechanical performance achieved at a 60% steel slag content. The microstructural evolution characterized by SEM-EDS and XRD revealed that steel slag incorporation promotes the formation of AFt and densifies the gel network. In later curing stages, natural carbonation of Ca(OH)₂ and secondary hydration of reactive steel slag components produce CaCO₃ and additional C-S-H gel, which fill pores and significantly enhance long-term strength. A CNN-GRU-Attention model was developed to predict the unconfined compressive strength (UCS) and splitting tensile strength (STS) of CSSS. In a single data split, the model achieved R² values of 0.9875 for UCS and 0.9911 for STS, with RMSEs of 0.2577 MPa and 0.0234 MPa, and MAEs of 0.2059 MPa and 0.0184 MPa, outperforming all benchmark models. Under rigorous 5 × 5 repeated cross-validation, it maintained the highest average R² (UCS: 0.9417, STS: 0.9329) and the lowest error metrics, confirming its robustness and generalization capability. SHAP and Pearson correlation analyses identified cement content as the primary strength determinant, while steel slag content exhibited a threshold effect, highlighting the importance of prudent gradation control in practical engineering. This study provides both a theoretical foundation and a methodological framework for analyzing variable interactions and predicting the strength development of CSSS. Full article