Search Results (303)

Power batteries are one of the important components of electric vehicles, but the manufacturing process of vehicle power batteries is complex and diverse. Traditional scheduling methods face challenges such as low production efficiency and inadequate quality control in complex production environments. To address these issues, a multi-objective optimization model with makespan, total machine load, and processing quality as the established objectives, and a Multi-objective Particle Swarm Energy Valley Optimization (MPSEVO) is proposed to solve the problem. MPSEVO integrates the advantages of Multi-objective Particle Swarm Optimization (MOPSO) and Energy Valley Optimization (EVO). In this algorithm, the particle stability level is combined in MOPSO, and different update strategies are used for particles of different stability to enhance both the convergence and diversity of the solutions. Furthermore, a local search strategy is designed to further enhance the algorithm to avoid the local optimal solutions. The Hypervolume ($HV$) and Inverted Generational Distance ($IGD$) indicators are often used to evaluate the convergence and diversity of multi-objective algorithms. The experimental results show that MPSEVO’s $HV$ and $IGD$ indicators are better than other algorithms in 10 computational experiments, which verifies the effectiveness of the proposed strategy and algorithm. The proposed method is also applied to solve the actual battery workshop scheduling problem. Compared with MOPSO, MPSEVO reduces the total machine load by 7 units and the defect rate by 0.05%. In addition, the effectiveness of each part of the improved algorithm was analyzed by ablation experiments. This paper provides some ideas for improving the solution performance of MOPSO, and also provides a theoretical reference for enhancing the production efficiency of the vehicle power battery manufacturing workshop. Full article

The stamp technique with bulk-fill composites aims to enhance occlusal surface replication in Class I restorations. Limited research exists on its void formation and volumetric changes. This study measures internal and external voids as well as volumetric changes in occlusal surfaces for both the stamp and conventional bulk-fill techniques. Materials and methods: Twenty-four permanent molars were divided into two groups (n = 12 each): Group 1 (conventional bulk fill) and Group 2 (stamp technique with bulk-fill composite). Standardized Class I cavities were prepared and restored using Tetric EvoCeram^® Bulk Fill composite. Micro-CT scanning was performed before and after restoration to quantify internal and external void percentages and volumetric changes. An independent samples t-test (α = 0.05) was used to compare void percentages and volumetric changes between groups. Results: The mean internal void percentage was similar between groups (Group 1: 0.38 ± 0.22%; Group 2: 0.39 ± 0.30%; p = 0.914), indicating comparable internal adaptation. Group 2 showed a significantly higher external void percentage (17.59 ± 1.76%) compared to Group 1 (9.05 ± 1.98%; p < 0.001), attributed to the stamp technique’s precise replication of occlusal micromorphology, misinterpreted as porosity by analysis software. Fractal dimension analysis revealed that the stamp technique resulted in the formation of a more complex structure. Restoration volumes (Group 1: 34.10 ± 8.09 mm³; Group 2: 35.52 ± 4.80 mm³; p = 0.639) and volumetric changes (Group 1: 5.91 ± 2.72 mm³; Group 2: 4.64 ± 1.31 mm³; p = 0.199) showed no significant differences. in conclusion, the stamp technique produced internal void percentages comparable to the conventional bulk-fill method in Class I restorations. The significantly higher external void percentage in the stamp technique group was due to the accurate replication of occlusal micromorphology, which was detected as porosity by analysis software. No significant differences were observed in volumetric changes of the occlusal surface before and after restoration between the two techniques, supporting the clinical viability of the stamp technique for precise occlusal restorations. Full article

This study evaluated the influence of different surface treatments and composite materials on the microtensile bond strength (μTBS) of repaired aged bulk-fill resin composite restorations, aligning with the principles of minimal intervention dentistry. Seventy-two specimens of bulk-fill resin composite (Tetric EvoCeram) were prepared, sectioned into bars (1 × 1 × 5 mm), and subjected to thermocycling to simulate aging. Specimens were randomly allocated into six groups (n = 12) based on surface treatment and repair material: phosphoric acid etching followed by repair with either Tetric EvoCeram (Group 1) or Filtek Z350 XT (Group 2); diamond bur roughening followed by repair with Tetric EvoCeram (Group 3) or Filtek Z350 XT (Group 4); and air abrasion followed by repair with Tetric EvoCeram (Group 5) or Filtek Z350 XT (Group 6). μTBS testing was performed using a universal testing machine, and failure patterns were examined under a stereomicroscope at 40× magnification. The highest bond strength values were observed in Groups 5 and 6 (air abrasion), followed by Group 3 (diamond bur). Groups 1, 2, and 4 showed significantly lower bond strength values (p < 0.05). No significant differences in failure modes were reported across groups. These findings suggest that air abrasion is a superior surface treatment for repairing aged bulk-fill resin composites, as it significantly enhanced μTBS compared to phosphoric acid etching and diamond bur roughening. Full article

Background: Major geopolitical events and structural shocks are thought to play a significant role in shaping HIV epidemics by influencing individual behaviours, reshaping social networks, and impacting HIV prevention and treatment programs. Here, we describe individual-level measures of estimated time since HIV infection (ETI) from viral next-generation sequencing data among female sex workers and their clients in relation to significant geopolitical events in Ukraine. Methods: The Dynamics Study is a cross-sectional integrated biological and behavioural survey conducted among female sex workers and their clients in Dnipro, Ukraine (December 2017 to March 2018). We were able to successfully sequence a portion of the HIV pol gene on dried blood spot specimens among n = 5/9 clients and n = 5/16 female sex workers who tested positive for HIV (total n = 10/25) using an in-house drug resistance genotyping assay. The “HIV EVO” Intrapatient HIV Evolution web-based tool was used to infer ETI from viral diversity. Results: The median ETIs for female sex workers and their clients were 5.4 years (IQR = 2.9, 6.6) and 6.5 years (IQR = 5.4, 10.8), respectively. Nearly all HIV acquisition events (n = 7/10; 70%) were estimated to have occurred between the Great Recession (2008–2009) and the War in Donbas (May 2014–February 2022). In general, ETI suggests that HIV acquisition occurred earlier among clients (2012 [IQR = 2007, 2013]) compared to sex workers (2013 [IQR = 2012, 2016]). Conclusion: Our findings suggest that most HIV acquisition in this small subset of female sex workers and clients living with HIV occurred during periods of economic decline. Molecular studies on timing of HIV acquisition against timing of major geopolitical events offer a novel way to contextualize how such events may shape transmission patterns. Full article

Background: This study aimed to evaluate the cytotoxicity of bulk-fill composite materials compared to conventional compomers on stem cells from human exfoliated deciduous teeth. Methods: 90 standardized resin composite discs (4 mm thick, 4 mm diameter) were fabricated using a 3D-printed plate, comprising four bulk-fill composites (SDR, Tetric EvoCeram Bulk-Fill, VisCalor Bulk, Cention-N) and one compomer (Dyract XP). Samples were polymerized per the manufacturer’s instructions and sterilized. Stem cells were isolated from the pulp of exfoliated primary teeth. Cells were cultured and exposed to extracts of the composite materials soaked in culture medium for 24 h. Cytotoxicity was assessed using the MTT colorimetric assay, measuring cell viability via mitochondrial activity, and the Annexin V assay, quantifying apoptosis and necrosis via flow cytometry. Statistical analysis was performed using ANOVA and Tukey post hoc tests. Results: All materials significantly reduced cell viability compared to the control (p < 0.05), with optical density values indicating high cytotoxicity. Tetric EvoCeram exhibited the lowest necrosis and apoptosis levels, while Dyract XP showed the highest necrosis. Statistical analysis revealed no significant cytotoxicity differences among most bulk-fill composites (p < 0.05). Conclusion: Bulk-fill composites and conventional compomer tested exhibit comparable and significant cytotoxic effects on stem cells from human exfoliated primary teeth pulp. While these materials offer clinical advantages in pediatric dentistry due to ease and speed of application, their use underscores the dilemma of balancing operative efficiency with biological safety, and their cytotoxic profiles should be taken into consideration prior to application. Full article

The feature selection (FS) procedure is a critical preprocessing step in data mining and machine learning, aiming to enhance model performance by eliminating redundant features and reducing dimensionality. The Energy Valley Optimizer (EVO), inspired by particle physics concepts of stability and decay, offers a novel metaheuristic approach. This study introduces an enhanced binary version of EVO, termed Improved Binarization in the Energy Valley Optimizer with Fractional Chebyshev Transformation (IBEVO-FC), and specifically designed for feature selection challenges. IBEVO-FC incorporates several key advancements over the original EVO. Firstly, it employs a novel fractional Chebyshev transformation function to effectively map the continuous search space of EVO to the binary domain required for feature selection, leveraging the unique properties of fractional orthogonal polynomials for improved binarization. Secondly, the Laplace crossover method is integrated into the initialization phase to improve population diversity and local search capabilities. Thirdly, a random replacement strategy is applied to enhance exploitation and mitigate premature convergence. The efficacy of IBEVO-FC is rigorously evaluated on 26 benchmark datasets from the UCI Repository and compared against 7 contemporary wrapper-based feature selection algorithms. Statistical analysis confirms the competitive performance of the proposed IBEVO-FC method in terms of classification accuracy and feature subset size. Full article

This study focused on developing emulsion-coated active papers with antifungal properties to extend the shelf life of tomatoes during home storage, thereby reducing food waste in households. First, a mixture of essential oils (EO_mix)—composed of 33.3% oregano and 66.7% cinnamon leaf oils—was optimised through a mixture design and emulsified with cationic starches. Based on their stability and efficacy, two different emulsions containing 10% Tween 80, 6–8% EO_mix, and HI-CAT or EVO cationic starches (82 or 84%, respectively) were selected and applied on paper. Then, the antifungal performance of the coated papers was tested in culture media against Botrytis cinerea, demonstrating strong antifungal activity in the vapour phase, effective for up to 31 days at 4 °C. In tests conducted with fresh tomatoes, the active papers improved fruit appearance and significantly reduced mould growth while maintaining overall sensory quality, indicating that these materials could extend tomato shelf life and thus offer a promising, low-cost, and biodegradable solution to reduce fruit waste at the consumer level, combining effective antifungal protection with good sensory performance in real-use conditions. Full article

The aim of this study was to evaluate (a) the degree of conversion (DC%), (b) film thickness, and (c) the effect of film thickness on DC% in modern light-cured resin composite restoratives [Filtek Universal (F), Clearfil Majesty ES 2 Universal (M), Tetric EvoCeram (T) and Viscalor (V)] used for luting composite onlays before/after preheating. For (a), the luting composites placed at 150 μm film thickness under the onlays (4 mm thickness, 2.9% transmittance) were light-cured for 120 s (3 × 40 s top, buccal, lingual sites) before and after preheating (54 °C/5 min-F,M,T and 65 °C/30 s-V). The DC% was measured at central, middle and side locations along the median in-length axis by ATR-FTIR spectroscopy. Specimens polymerized without onlays (40 s, top) served as controls. For (b), film thickness was measured employing a modified ISO 4049 standard (37 °C plate temperature, 5 N load) before and after preheating, using a dual-cured resin luting agent as control. For (c), onlays were luted with preheated T at 150 and 350 μm film thickness and light-cured for 2 × (3 × 40) s and 3 × (3 × 40) s, employing directly irradiated specimens (60 s, 120 s) as controls. For (a), significant differences were found in F and T before and after preheating. Before preheating, significant differences were registered between F–T, F–M, F–V and V–T, whereas after they were registered between F–M, F–T and F–V. All these values were significantly lower than the controls. For (b), significantly lower film thickness was recorded after preheating (−16.1–−33.3%, highest in V), with a ranking of F, M > V > T (before) and F, M > T, V (after). All values were significantly higher than the control. For (c), increased exposure improved DC% in the greater spacer group, with the controls providing superior values. It can be concluded that the use of modern highly filled composites as luting agents for low translucency onlays may result in suboptimal polymerization and film thickness, warranting caution. Full article

Background. Physicomechanical properties and clinical service of bulk-fill composites depend on their adequate polymerization and depth of cure. Some manufacturers claim that these composites can be adequately cured when used in bulks exceeding 4 mm. Objective. The aim of this study was to compare Vickers microhardness (VMH) and depth of cure (DOC) of six contemporary bulk-fill resin composites at depths of 4 mm and 6 mm. Material and methods. Six bulk-fill composites were evaluated in this study: 1. Tetric EvoCeram Bulk (Ivoclar Vivadent, Schaan, Liechtenstein), (TEC); 2. Filtek Bulk Fill Posterior (3M ESPE Dental Products Division, St. Paul, MN, USA), (FBF); 3. Filtek One Bulk Fill (3M ESPE Dental Products Division, St. Paul, MN, USA, (FOB); 4. SonicFill 2 (Kerr, Orange, CA, USA), (SF2); 5. Admira Fusion X-tra (Voco, GmbH, Cuxhaven, Germany), (AFX); 6. GrandioSO X-tra (Voco, GmbH, Cuxhaven, Germany), (GSX). The 18 specimens (3 of each composite) were prepared in split Teflon moulds of 4 mm diameter and 6 mm thickness. All composites were cured in standard mode for 20 s using LED LCU (D-Light Duo, RF-Pharmaceuticals Sarl, Geneva, Switzerland; 1200–1300 mW/cm). The VMH was measured using a digital Micro Hardness Tester Shimadzu (HMV-2T E, Shimadzu Corporation, Kyoto, Japan). A 50 g (0.5 N) load force was applied for 30 s. Each specimen was measured at five places selected by chance at each level (N = 15). The hardness ratio or DOC was calculated for all samples as the ratio of bottom and surface microhardness at levels of 4 and 6 mm. Data were analysed using one-way ANOVA and Tukey’s post hoc test. Results. Significant reduction in VMH was observed for all tested materials when comparing top surface and bottom (p < 0.01). The highest VMH was obtained for GSX and AFX, and the lowest for TEC. The results show that the degree of polymerization was adequate for all tested materials at a depth of 6 mm, since the hardness ratio exceeded 0.80 in all cases. The hardness ratio at 4 mm was high for all tested composites ranging from 0.91 for TEC to 0.98 for GSX. All composites showed adequate DOC at the bottom of the 6 mm bulk samples. However, the hardness ratio was the highest for Admira Fusion X-tra (0.96) and GrandioSO X-tra (0.97). Conclusions. All tested materials showed a significant decrease in microhardness from the top surface to the bottom. The DOC was adequate for all bulk-fill composites at a depth of 6 mm cured under standard mode for 20 s. All bulk-fill resin composites evaluated in this study can be used in bulk, up to 6 mm. Full article

Background and Objectives: Posterior chamber phakic implantable contact lenses (Phakic-ICL) are widely used for refractive correction due to their efficacy and safety, including minimal corneal endothelial cell loss. The Collamer-based EVO+ Visian implantable contact lens (ICL), manufactured from Collamer, which is a blend of collagen and hydroxyethyl methacrylate (HEMA), has demonstrated excellent long-term biocompatibility and optical clarity. Recently, hydrophilic acrylic Phakic-ICLs, such as the Implantable Phakic Contact Lens (IPCL), have been introduced. This study investigated the material differences among Phakic-ICLs and their interaction with fibronectin (FN), which has been reported to adhere to intraocular lens (IOL) surfaces following implantation. The aim was to compare Collamer, IPCL, and LENTIS lenses (used as control) in terms of FN distribution and cell adhesion using a small number of explanted Phakic-ICLs. Materials and Methods: Three lens types were analyzed: a Collamer Phakic-ICL (EVO+ Visian ICL), a hydrophilic acrylic IPCL, and a hydrophilic acrylic phakic-IOL (LENTIS). FN distribution and cell adhesion were evaluated across different regions of each lens. An in vitro FN-coating experiment was conducted to assess its effect on cell adhesion. Results: All lenses demonstrated minimal FN deposition and cellular adhesion in the central optical zone. A thin FN film was observed on the haptics of Collamer lenses, while FN adhesion was weaker or absent on IPCL and LENTIS surfaces. Following FN coating, Collamer lenses supported more uniform FN film formation; however, this did not significantly enhance cell adhesion. Conclusions: Collamer, which contains collagen, promotes FN film formation. Although FN film formation was enhanced, the low cell-adhesive properties of HEMA resulted in minimal cell adhesion even with FN presence. This characteristic may contribute to the long-term transparency and biocompatibility observed clinically. In contrast, hydrophilic acrylic materials used in IPCL and LENTIS demonstrated limited FN interaction. These material differences may influence extracellular matrix protein deposition and biocompatibility in clinical settings, warranting further investigation. Full article

This research proposes an integrated lighting and solar shading strategy to improve energy efficiency and user comfort in a retrofit project in a temperate-humid climate. The study examines a future library addition to an existing faculty building in Bursa, featuring highly glazed façades (77% southwest, 81% northeast window-to-wall ratio), an open-plan layout, and situated within an unobstructed low-rise campus environment. Trade-offs between daylight availability, heating, cooling, lighting energy use, and visual and thermal comfort are evaluated through integrated lighting (DIALux Evo), climate-based daylight (CBDM), and energy simulations (DesignBuilder, EnergyPlus, Radiance). Fifteen solar shading configurations—including brise soleil, overhangs, side fins, egg crates, and louvres—are evaluated alongside a daylight-responsive LED lighting system that meets BS EN 12464-1:2021. Compared to the reference case’s unshaded glazing, optimal design significantly improves building performance: a brise soleil with 0.4 m slats at 30° reduces annual primary energy use by 28.3% and operational carbon emissions by 29.1% and maintains thermal comfort per ASHRAE 55:2023 Category II (±0.7 PMV; PPD < 15%). Daylight performance achieves 91.5% UDI and 2.1% aSE, with integrated photovoltaics offsetting 129.7 kWh/m² of grid energy. This integrated strategy elevates the building’s energy class under national benchmarks while addressing glare and overheating in the original design. Full article

This study evaluated the efficacy of various cleaning protocols on two nickel–titanium (NiTi) file systems—RaCe EVO(RE) and EdgeFile X7(EE)—using scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDX). Eighty-four NiTi files (42RE, 42EE) were divided into seven groups (n = 12), including a group with unused, sterilized files and a group of used files without cleaning. The remaining files were subjected to simulated clinical use, followed by different cleaning methods, such as soaking in sodium hypochlorite (NaOCl), ethanol wiping (with or without magnification), enzymatic spray, and enzymatic solution. SEM images were imported into ImageJ to quantify surface changes, while EDX assessed elemental composition. The p-value was set to ≤0.05 for significance. Apart from the unused files, calcium and phosphorus—indicators of dentin debris—were present in all groups, especially those cleaned with enzymatic spray (p ≤ 0.0001). Their percentage in RE files soaked in NaOCl or wiped with ethanol was statistically lower than the positive control (p ≤ 0.0001). Post-use, all files showed significantly higher surface asymmetry in Groups 2 and 6 (p = 0.001). Cleaning efficacy depends on the type of NiTi file. RE files responded well to both wiping and soaking, while EE required soaking for effective debris removal. Enzymatic spray was ineffective. Full article

Automated generative design is increasingly used across engineering disciplines to accelerate innovation and reduce costs. Generative design offers the prospect of simplifying manual design tasks by exploring the efficacy of solutions automatically. However, existing generative design frameworks rely heavily on expensive optimisation procedures and often produce customised solutions, lacking reusable generative rules that transfer across different problems. This work presents a bioinspired generative design algorithm utilising the concept of evolutionary development (EvoDevo). This evolves a set of developmental rules that can be applied to different engineering problems to rapidly develop designs without the need to run full optimisation procedures. In this approach, an initial design is decomposed into simple entities called cells, which independently control their local growth over a development cycle. In biology, the growth of cells is governed by a gene regulatory network (GRN), but there is no single widely accepted model for this in artificial systems. The GRN responds to the state of the cell induced by external stimuli in its environment, which, in this application, is the loading regime on a bridge truss structure (but can be generalised to any engineering structure). Two GRN models are investigated: graph neural network (GNN) and graph-based Cartesian genetic programming (CGP) models. Both GRN models are evolved using a novel genetic search algorithm for parameter search, which can be re-used for other design problems. It is revealed that the CGP-based method produces results similar to those obtained using the GNN-based methods while offering more interpretability. In this work, it is shown that this EvoDevo approach is able to produce near-optimal truss structures via growth mechanisms such as moving vertices or changing edge features. The technique can be set up to provide design automation for a range of engineering design tasks. Full article

Reparative tertiary dentinogenesis requires the recruitment and odontogenic differentiation of dental pulp stem cells (DPSCs). Extracellular vesicles (EVs) as bioactive molecules have gained attention in regenerative medicine for their ability to mediate tissue repair through intercellular communication, influencing cell recruitment, proliferation, and differentiation. This study aimed to evaluate the effects of EVs on DPSC homing and odontogenic differentiation for dentin regeneration. DPSC-derived EVs were cultured in either growth (EV-G) or odontogenic differentiation (EV-O) conditions and isolated using a modified precipitation method. EVs were characterized by nanoparticle tracking analysis, scanning electron microscopy, antibody array, and cellular uptake assay. Treatment with 5 × 10⁸ EVs/mL significantly enhanced DPSC chemotaxis and proliferation compared with a no-treatment control and a lower dosage of EV (5 × 10⁷ EVs/mL). Gene expression and biochemical analyses revealed that EV-O up-regulated odontogenic markers including collagen type 1A1 (COL1A1), runt-related transcription factor 2 (RUNX2), and alkaline phosphatase (ALP). EV-O enhanced dentin regeneration by approximately 55% over vehicle controls in a rabbit partial dentinotomy/pulpotomy model. We identified key microRNAs (miR-21-5p, miR-221-3p, and miR-708-3p) in EV-O involved in cell homing and odontogenesis. In conclusion, our EV-based cell homing and odontogenic differentiation strategy has significant therapeutic potential for dentin regeneration. Full article

Oral squamous cell carcinoma (OSCC), an aggressive and poorly prognosed subtype of head and neck squamous cell carcinoma (HNSCC), has prompted urgent calls for innovative therapeutic approaches. Evodiamine (EVO), a natural alkaloid extracted from the Chinese herb Evodia rutaecarpa, has demonstrated significant potential in curbing tumor cell proliferation and slowing tumor expansion. However, its specific effects on cell senescence within the context of OSCC have remained shrouded in uncertainty. This study delves into the mechanisms of EVO’s impact on OSCC by harnessing databases such as the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP), The Cancer Genome Atlas (TCGA), the Gene Expression Omnibus (GEO), and CellAge to pinpoint potential targets and carry out in-depth bioinformatics analysis. The findings reveal that EVO can markedly enhance the expression of the androgen receptor (AR) in OSCC cells, inducing cellular senescence and thereby inhibiting tumor progression. Furthermore, the research indicates that AR expression is considerably lower in OSCC tissues than in normal tissues. This low expression of AR in tumor tissues is closely associated with advanced clinical stages and unfavorable prognoses in HNSCC patients. These discoveries open up new avenues for therapeutic strategies, and suggest that AR holds promise as a potential therapeutic target for OSCC, and EVO may amplify its antitumor effects by enhancing AR-mediated cellular senescence in the treatment of OSCC. Full article