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19 pages, 1906 KB  
Article
LADOS: Aerial Imagery Dataset for Oil Spill Detection, Classification, and Localization Using Semantic Segmentation
by Konstantinos Gkountakos, Maria Melitou, Konstantinos Ioannidis, Konstantinos Demestichas, Stefanos Vrochidis and Ioannis Kompatsiaris
Data 2025, 10(7), 117; https://doi.org/10.3390/data10070117 - 14 Jul 2025
Viewed by 1166
Abstract
Oil spills on the water surface pose a significant environmental hazard, underscoring the critical need for developing Artificial Intelligence (AI) detection methods. Utilizing Unmanned Aerial Vehicles (UAVs) can significantly improve the efficiency of oil spill detection at early stages, reducing environmental damage; however, [...] Read more.
Oil spills on the water surface pose a significant environmental hazard, underscoring the critical need for developing Artificial Intelligence (AI) detection methods. Utilizing Unmanned Aerial Vehicles (UAVs) can significantly improve the efficiency of oil spill detection at early stages, reducing environmental damage; however, there is a lack of training datasets in the domain. In this paper, LADOS is introduced, an aeriaL imAgery Dataset for Oil Spill detection, classification, and localization by incorporating both liquid and solid classes of low-altitude images. LADOS comprises 3388 images annotated at the pixel level across six distinct classes, including the background. In addition to including a general oil class describing various oil spill appearances, LADOS provides a detailed categorization by including emulsions and sheens. Detailed examination of both instance and semantic segmentation approaches is illustrated to validate the dataset’s performance and significance to the domain. The results on the test set demonstrate an overall performance exceeding 66% mean Intersection over Union (mIoU), with specific classes such as oil and emulsion to surpass 74% of IoU part of the experiments. Full article
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21 pages, 38876 KB  
Article
Computational Fluid Dynamics Analysis of Ballast Water Treatment System Design
by Andro Rak, Tomislav Mrakovčić, Goran Mauša and Lado Kranjčević
J. Mar. Sci. Eng. 2025, 13(4), 743; https://doi.org/10.3390/jmse13040743 - 8 Apr 2025
Viewed by 715
Abstract
The effective management of ships’ ballast water is critical for preventing the spread of invasive species. Despite advancements in UV-based ballast water treatment systems (BWTSs), achieving a uniform flow distribution within UV reactors (UVRs) remains challenging due to the spatial constraints of ships. [...] Read more.
The effective management of ships’ ballast water is critical for preventing the spread of invasive species. Despite advancements in UV-based ballast water treatment systems (BWTSs), achieving a uniform flow distribution within UV reactors (UVRs) remains challenging due to the spatial constraints of ships. This study employs computational fluid dynamics (CFD) to analyze turbulent seawater flow in a real-case BWTS installed on a self-discharging bulk carrier. The flow uniformity at UVR inlets and the volume flow rate (Q) distribution between parallel reactors are evaluated at nominal flow rates of 1000, 1900, and 2000 m3/h. The results indicate significant disparities at maximum capacity (2000 m3/h), with the starboard configuration exceeding the recommended Q per UVR by 4.95%, thus requiring operational adjustments. Six geometric modifications are assessed, revealing that optimized pipeline bends and T-junction designs (e.g., ST_3 and ST_4) improve velocity uniformity and maintain the relative Q distribution errors below 8.5%. This study identifies vortical structures generated by sharp geometrical transitions as primary contributors to flow instability. By bridging CFD insights with practical engineering constraints, this work provides feasible recommendations for retrofitting existing BWTSs and designing future systems, ultimately enhancing treatment efficacy, reducing UV lamp wear, and supporting compliance with International Maritime Organization (IMO) standards. Full article
(This article belongs to the Section Ocean Engineering)
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14 pages, 2047 KB  
Article
Molecular Dynamics Simulation of CNT Reinforced Cement: A Step Toward Sustainable Construction
by Rosario G. Merodio-Perea, María-José Terrón-López and Isabel Lado-Touriño
Sustainability 2025, 17(7), 3185; https://doi.org/10.3390/su17073185 - 3 Apr 2025
Cited by 1 | Viewed by 574
Abstract
This study explores the potential of carbon nanotubes (CNTs) to enhance cement mechanical properties, aiming to develop more sustainable materials and reduce the industry’s carbon footprint. Using molecular dynamics (MDs) simulations, the effect of pristine and carboxyl-functionalized single-walled CNT incorporation on the mechanical [...] Read more.
This study explores the potential of carbon nanotubes (CNTs) to enhance cement mechanical properties, aiming to develop more sustainable materials and reduce the industry’s carbon footprint. Using molecular dynamics (MDs) simulations, the effect of pristine and carboxyl-functionalized single-walled CNT incorporation on the mechanical properties of 11 Å tobermorite, a model for calcium–silicate–hydrate (CSH), was analyzed. The results demonstrated a significant increase in the elastic modulus (E) of the composite, with CNT content directly influencing this enhancement. Specifically, E increased from 77.05 GPa to 81.93 GPa upon the incorporation of pristine CNTs and further increased to 97.87 GPa with the introduction of carboxyl-functionalized CNTs. Composites containing functionalized CNTs exhibited a more pronounced increase in E, as the carboxyl groups formed hydrogen bonds with the tobermorite structure, thereby reinforcing interactions and improving mechanical properties. Thus, increasing functionalization allows for lower reinforcement content, reducing costs and CNT aggregation, as observed in experimental studies. These findings underscore the potential of functionalized CNTs to strengthen cementitious materials, offering an alternative to traditional additives. This approach could contribute to reducing the carbon emissions associated with cement production, thereby supporting the development of more sustainable and environmentally friendly cement alternatives. Full article
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16 pages, 2860 KB  
Article
Molecular Dynamics Simulations of Sustainable Green Binders for Metal Injection Molding
by Isabel Lado-Touriño and Rosario G. Merodio-Perea
Sustainability 2025, 17(5), 2263; https://doi.org/10.3390/su17052263 - 5 Mar 2025
Viewed by 947
Abstract
Polylactic acid (PLA) and poly(ethylene adipate) (PEA) are biodegradable, biobased polymers renowned for their versatility and environmental advantages. This study explores the potential of PLA-PEA blends as green binders in the metal injection molding (MIM) process, a crucial manufacturing technique for producing complex [...] Read more.
Polylactic acid (PLA) and poly(ethylene adipate) (PEA) are biodegradable, biobased polymers renowned for their versatility and environmental advantages. This study explores the potential of PLA-PEA blends as green binders in the metal injection molding (MIM) process, a crucial manufacturing technique for producing complex metal components. Substituting conventional, environmentally harmful binders with these blends offers a sustainable strategy to reduce the environmental footprint of MIM. Achieving compatibility between binder components is essential to ensure optimal molding performance in this application. To evaluate this compatibility, molecular dynamics (MD) simulations were employed to analyze the interaction and miscibility of both polymers. Simulations across various blend compositions and temperatures consistently yielded negative Flory–Huggins interaction parameters, demonstrating strong miscibility between PLA and PEA. Notably, blends with lower PEA content exhibited the most favorable compatibility. Radial distribution function analyses further confirmed these results, revealing enhanced miscibility with lower-molecular-weight PEA. This study underscores the potential of PLA-PEA blends as sustainable alternative binders in MIM, advancing the use of biobased materials in energy-efficient and eco-friendly industrial processes. By integrating PLA into MIM, this research contributes to the development of greener engineering practices and highlights the viability of sustainable material solutions for industrial applications. Full article
(This article belongs to the Special Issue Resource Sustainability: Sustainable Materials and Green Engineering)
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17 pages, 1429 KB  
Article
Sequential Fermentation with Non-Saccharomyces Yeasts Improves the Chemical and Sensory Characteristics of Albariño and Lado Wines
by Estefanía García-Luque, Rebeca González, Rafael Cao, Elvira Soto and Pilar Blanco
Fermentation 2025, 11(2), 73; https://doi.org/10.3390/fermentation11020073 - 3 Feb 2025
Cited by 1 | Viewed by 1465
Abstract
The application of non-Saccharomyces yeast in mixed fermentations with Saccharomyces cerevisiae is a useful tool to enhance wine quality. In this study, Metschnikowia fructicola Mf278 and Pichia kluyveri Pk1 were used in sequential fermentations with S. cerevisiae XG3 to ferment grape musts [...] Read more.
The application of non-Saccharomyces yeast in mixed fermentations with Saccharomyces cerevisiae is a useful tool to enhance wine quality. In this study, Metschnikowia fructicola Mf278 and Pichia kluyveri Pk1 were used in sequential fermentations with S. cerevisiae XG3 to ferment grape musts from Albariño and Lado. The development of fermentations was monitored by daily measurements of density and temperature, and sampling at the beginning, tumultuous, and final stages for microbiological control. The basic chemical parameters of wine were determined using the OIV official methodology, whereas the fermentative aroma compounds were quantified by GC–MS. M. fructicola Mf278 and P. kluyveri Pk1 were the predominant yeasts at the initial stages of sequential fermentations but, after the addition of S. cerevisiae XG3, they rapidly declined. A codominance of different S. cerevisiae strains was observed at the middle and final stages of fermentation. At the chemical level, Mf278 lowered the volatile acidity and increased the glycerol content of wines. Moreover, M. Mf278 and Pk1 increased the content of fermentative esters and fatty acids of wines. These compounds contribute fruity and floral notes to the wines that stood out over wines made only with S. cerevisiae, and were better valued at the sensory level. Full article
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17 pages, 2205 KB  
Article
Exploring Metal Cluster Interactions with Functionalized Graphene via Molecular Dynamics Simulation
by Miriam Roldán-Matilla, Arisbel Cerpa-Naranjo and Isabel Lado-Touriño
C 2024, 10(4), 107; https://doi.org/10.3390/c10040107 - 18 Dec 2024
Viewed by 1577
Abstract
This study investigates the interaction between copper (Cu) and silver (Ag) clusters and graphene-based materials using molecular dynamics simulations. It focuses on how graphene oxidation and aminated polyethylene glycol (PEG-NH2) functionalization influence interaction strength and cluster dynamics. The analysis includes pristine [...] Read more.
This study investigates the interaction between copper (Cu) and silver (Ag) clusters and graphene-based materials using molecular dynamics simulations. It focuses on how graphene oxidation and aminated polyethylene glycol (PEG-NH2) functionalization influence interaction strength and cluster dynamics. The analysis includes pristine graphene (PG), low-oxidized graphene oxide (GOL), and PEGylated graphene oxide (GO-PEG-NH2). The results reveal that clusters on PG exhibit high mobility, while GO-PEG-NH2 significantly restricts mobility due to strong interactions, as evidenced by highly negative interaction energies. GO-PEG-NH2 systems also display pronounced subdiffusive behavior (α < 1), indicating strong binding and constrained motion. These findings underscore the critical role of PEG-NH2 functionalization in controlling cluster diffusion, paving the way for innovative designs in biomedical and catalytic nanocarrier applications. Full article
(This article belongs to the Special Issue Carbon Nanohybrids for Biomedical Applications (2nd Edition))
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11 pages, 953 KB  
Article
Control of Green Mold and Sour Rot in Mandarins by Postharvest Application of Natamycin and an Allium Extract
by Elena Pérez Faggiani, Gerónimo Fernandez, Mariángeles Cocco, Mauricio Sbres, Oribe Blanco and Joanna Lado
Plants 2024, 13(23), 3428; https://doi.org/10.3390/plants13233428 - 6 Dec 2024
Cited by 2 | Viewed by 1226
Abstract
The efficacy of natamycin (Fruitgard Nat 20) and Proallium (an extract of allium including propyl thiosulfinate oxide (PTSO)) against sour rot and green mold in mandarins was evaluated under controlled and commercial conditions. The study involved artificial inoculation of Nova, Tango, Orri, Afourer, [...] Read more.
The efficacy of natamycin (Fruitgard Nat 20) and Proallium (an extract of allium including propyl thiosulfinate oxide (PTSO)) against sour rot and green mold in mandarins was evaluated under controlled and commercial conditions. The study involved artificial inoculation of Nova, Tango, Orri, Afourer, Murcott, and Nules Clementine mandarins with isolates of Penicillium digitatum resistant to imazalil and pyrimethanil and an isolate of Geotrichum citri-aurantii susceptible to propiconazole fungicides. Under laboratory conditions, natamycin applied at 1500 µg mL−1 significantly reduced green mold by 61.2% in Orri and sour rot by 62.8% in Nova and 80% in Tango. Increasing the concentration to 2000 µg mL−1 further improved control of sour rot in Nova to 92.8%. In commercial trials, natamycin at 1500 µg mL−1 was ineffective on Afourer; however, 2000 µg mL−1 reduced sour rot by 39% on Nules Clementine. Proallium (12–16 µg mL−1 PTSO) applied under controlled conditions effectively reduced green mold by 33% in Nova and 31% in Nules Clementine, and sour rot by 19%, 41%, and 36% in Nules Clementine, Nova, and Afourer, respectively. Under commercial conditions, using the same dose of Proallium, there was a 51.5% reduction in the incidence of imazalil and pyrimethanil-resistant P. digitatum strains and a 36.5% reduction in sour rot. Both natamycin and PTSO showed promising results for managing green mold caused by fungicide-resistant strains, but further research is needed to optimize control of sour rot in mandarins. Full article
(This article belongs to the Special Issue Postharvest Quality and Physiology of Vegetables and Fruits)
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14 pages, 1485 KB  
Article
Exploration of Strawberry Fruit Quality During Harvest Season Under a Semi-Forcing Culture with Plants Nursed Without Chilling
by Paula Pedrozo, Bettina Lado, Ana Inés Moltini, Esteban Vicente and Joanna Lado
Plants 2024, 13(21), 3052; https://doi.org/10.3390/plants13213052 - 31 Oct 2024
Cited by 1 | Viewed by 1420
Abstract
Strawberry, a profitable crop, adapts well to diverse environments. This study evaluated the stability of fruit quality in different strawberry varieties produced as green plants without chilling during the harvest season in northern Uruguay. The focus was on understanding the impact of harvest [...] Read more.
Strawberry, a profitable crop, adapts well to diverse environments. This study evaluated the stability of fruit quality in different strawberry varieties produced as green plants without chilling during the harvest season in northern Uruguay. The focus was on understanding the impact of harvest date versus agricultural practices (different orchards and growers) on key fruit quality variables such as color, firmness, sugars, and acidity. Results indicated that the INIA Yrupé variety showed greater stability in external coloration and sugar content across harvest dates, suggesting that standardizing fruit coloration is viable under challenging conditions. INIA Guapa consistently met minimum requirements for soluble solids and ratio, with stable acidity and red coloration. Firmness was positively correlated with ratio (r = 0.81) and negatively impacted by rainfall 2–3 days before harvest. The findings suggest that harvest date has a more significant impact on INIA Yrupé fruit quality than growing conditions or practices, with variability observed in firmness and ratio but minimal variation in fruit coloration. These insights highlight the importance of harvest timing for maintaining stable fruit quality traits, which is crucial for breeding programs and ensuring consistent sensory quality and crop profitability. Full article
(This article belongs to the Special Issue Postharvest Quality and Physiology of Vegetables and Fruits)
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14 pages, 1236 KB  
Article
Diversity of Saccharomyces cerevisiae Yeast Strains in Granxa D’Outeiro Winery (DOP Ribeiro, NW Spain): Oenological Potential
by Pilar Blanco, Estefanía García-Luque, Rebeca González, Elvira Soto, José Manuel M. Juste and Rafael Cao
Fermentation 2024, 10(9), 475; https://doi.org/10.3390/fermentation10090475 - 13 Sep 2024
Cited by 1 | Viewed by 1342
Abstract
Yeasts play an essential role in the aroma and sensory profiles of wines. Spontaneous fermentations were carried out at the newly built winery of Granxa D’Outeiro. Yeasts were isolated from must at different stages of fermentation. Colonies belonging to Saccharomyces cerevisiae were characterised [...] Read more.
Yeasts play an essential role in the aroma and sensory profiles of wines. Spontaneous fermentations were carried out at the newly built winery of Granxa D’Outeiro. Yeasts were isolated from must at different stages of fermentation. Colonies belonging to Saccharomyces cerevisiae were characterised at the strain level by mtDNA-RFLPs. General chemical parameters and aroma profiles of the wines were determined using official OIV methodology and GC-MS analysis, respectively. The diversity of S. cerevisiae per fermentation ranged from 5 to 13 different strains depending on the grapevine variety. Out of 24 strains, strain B was the dominant yeast in most fermentations at different proportions, but strains D, E, and H also reached up to 25% of the total population in some fermentations. The yeast diversity was higher in the Lado fermentation than in those containing Treixadura. The chemical compositions of the wines revealed differences among them, with Loureira and Albariño wines showing the highest content of volatile compounds. The evaluation of their technological properties revealed the oenological potential of some strains of S. cerevisiae. The strains showing the best scores were selected to be used in future vintages to enhance the typicality of wines in the Granxa D’Outeiro winery. Full article
(This article belongs to the Special Issue Saccharomyces cerevisiae Strains and Fermentation: 2nd Edition)
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12 pages, 546 KB  
Article
Japanese Encephalitis Virus Surveillance in U.S. Army Installations in the Republic of Korea from 2021 to 2023
by Paula Lado, Gary P. Crispell, Sung Tae Chong, Myong Sun Kim, Ashley N. Esparza, Eric Zielinski, Akira Iwami, Kelly P. Williams, John J. Eads, Kei Jimbo, Dana N. Mitzel, Lee W. Cohnstaedt, Joshua B. Richardson, Jeffrey R. Kugelman and Craig A. Stoops
Pathogens 2024, 13(8), 705; https://doi.org/10.3390/pathogens13080705 - 20 Aug 2024
Cited by 3 | Viewed by 1555
Abstract
Japanese encephalitis is a disease caused by the Japanese encephalitis virus (JEV) and is a concern for U.S. military personnel stationed in the Republic of Korea (ROK). The recent literature reports a potential shift from GI to GV as the dominant genotype circulating [...] Read more.
Japanese encephalitis is a disease caused by the Japanese encephalitis virus (JEV) and is a concern for U.S. military personnel stationed in the Republic of Korea (ROK). The recent literature reports a potential shift from GI to GV as the dominant genotype circulating in east Asia. In the ROK, GV has been reported in a few Culex spp., but not in the main JEV vector, Cx. tritaeniorhynchus. The goal of this surveillance was to shed light on the current knowledge of the epidemiology of JEV in the ROK by analyzing mosquito collection data from three consecutive years, 2021–2023, and molecularly detecting and genotyping JEV in all Culex spp. collected in several military locations across the ROK. In this study, we detected only JEV GI in Cx. tritaeniorhynchus in 2021 samples. In contrast, all 2022 and 2023 positive samples were GV and detected in Cx. bitaeniorhynchus, Cx. orientalis, and Cx. pipiens. Results support a shift in JEV genotype in the ROK and suggest that for GV, Culex spp. other than Cx. tritaeniorhynchus may be playing an important role. Full article
(This article belongs to the Special Issue The Future of Vector-Borne Diseases in a Changing World)
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24 pages, 2521 KB  
Article
Moving Towards a Holistic Approach to Circular Cities: Obstacles and Perspectives for Implementation of Nature-Based Solutions in Europe
by Jelena Ristić Trajković, Verica Krstić, Aleksandra Milovanović, Cristina Sousa Coutinho Calheiros, Mirjana Ćujić, Milica Karanac, Jan K. Kazak, Sara Di Lonardo, Rocío Pineda-Martos, Mari Carmen Garcia Mateo, Dragan Milošević, Maria Milousi, Mihai Răzvan Niță, Stefania Anna Palermo, Patrizia Piro, Behrouz Pirouz, Zorina Siscan, Michele Turco, Mentore Vaccari, Nataša Atanasova, Guenter Langergraber, Rita Lado Ribeiro and Maja Đolićadd Show full author list remove Hide full author list
Sustainability 2024, 16(16), 7085; https://doi.org/10.3390/su16167085 - 18 Aug 2024
Cited by 4 | Viewed by 3126
Abstract
Nature-based solutions (NBS) are frequently implemented without taking the system’s perspective into account and with the main focus on technical and economic issues of implementation. This study was conducted to test the hypothesis on the potential synergistic effects between circularity and NBS to [...] Read more.
Nature-based solutions (NBS) are frequently implemented without taking the system’s perspective into account and with the main focus on technical and economic issues of implementation. This study was conducted to test the hypothesis on the potential synergistic effects between circularity and NBS to holistically tackle urban challenges. The main objective is to establish preliminary insights on the obstacles and perspectives of NBS integration and implementation, through a questionnaire set up by the network of experts gathered within the COST Action CA17133 Circular City. The following research questions arise: (i) what differences exist in the level of NBS application according to the variance of engaged countries; and (ii) what are the main obstacles and perspectives for the NBS implementation in order to holistically tackle urban challenges, enhancing the sustainable connection among urban environment, nature, and human well-being. To go beyond the current state-of-the-art and reflect on the research conducted within the Circular City Action, this study aims to open a multi-geographical academic dialogue across Europe and beyond and to move towards a holistic approach to circular cities. Accordingly, this study is: (1) multi-geographical and context-based, providing input for thirty-three EU countries and four non-EU countries to give an overview of the main obstacles and perspectives of NBS implementation, and (2) approach-directed, aiming to formulate a holistic approach to deal with societal challenges. This document intends to provide qualitative and quantitative insight into the potentials and obstacles of NBS implementation in Europe, as well as to motivate further discussion and research to achieve holistic and sustainable cities. Full article
(This article belongs to the Special Issue Sustainable Urban Planning: Biodiversity, Greening, and Forestry)
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23 pages, 2144 KB  
Article
Characterization of Biochar from Beach-Cast Seaweed and Its Use for Amelioration of Acid Soils
by Eliana Cárdenas-Aguiar, Gabriel Gascó, Marcos Lado, Ana Méndez, Jorge Paz-Ferreiro and Antonio Paz-González
Land 2024, 13(6), 881; https://doi.org/10.3390/land13060881 - 18 Jun 2024
Cited by 4 | Viewed by 2462
Abstract
Limited knowledge is available regarding biochar (BC) obtained from beach-cast seaweed, which needs to be characterized to better prospect its potential applications. Beach-cast seaweed BCs were produced at two temperatures (300 and 600 °C) and two residence times (1 and 3 h). First, [...] Read more.
Limited knowledge is available regarding biochar (BC) obtained from beach-cast seaweed, which needs to be characterized to better prospect its potential applications. Beach-cast seaweed BCs were produced at two temperatures (300 and 600 °C) and two residence times (1 and 3 h). First, an extensive characterization of feedstock and their derived BCs was performed. Then, an incubation experiment was conducted to investigate the potential of these BCs as ameliorants in two acidic soils, classified as Umbrisols. Proximate and elemental analyses suggested the presence of more stable C structures in BCs at 600 °C with a high C, fixed carbon (FC) and low H, O and volatile matter (VM); additionally, thermogravimetric graphics showed less weight loss. Some features from the raw material were present in biochars obtained at 300 °C but absent at 600 °C. Dissolved organic carbon was much lower in BCs pyrolyzed at 600 °C than at 300 °C, suggesting the higher aromaticity of the former, allowing more resistance to water extraction. The breakdown of proteinaceous soluble materials after pyrolysis was revealed by fluorescence. Residence time caused minor effects in biochar properties compared to temperature. The soil pH and macronutrient availability of the two studied soils increased after beach-cast seaweed biochar addition. Full article
(This article belongs to the Section Land, Soil and Water)
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18 pages, 821 KB  
Article
The Effects of Feedstock, Pyrolysis Temperature, and Residence Time on the Properties and Uses of Biochar from Broom and Gorse Wastes
by Eliana Cárdenas-Aguiar, Ana Méndez, Gabriel Gascó, Marcos Lado and Antonio Paz-González
Appl. Sci. 2024, 14(10), 4283; https://doi.org/10.3390/app14104283 - 18 May 2024
Cited by 8 | Viewed by 3946
Abstract
Biochar (BC), which can be produced from several feedstocks, has been widely studied. However, the BC derived from highly pyrolytic shrubs, such as broom and gorse, has been less frequently used and only partially characterized. These wastes, when used for the preparation of [...] Read more.
Biochar (BC), which can be produced from several feedstocks, has been widely studied. However, the BC derived from highly pyrolytic shrubs, such as broom and gorse, has been less frequently used and only partially characterized. These wastes, when used for the preparation of biochar, can fix carbon and contribute to environmental conservation, helping to achieve sustainable development objectives. Eight biochars from broom and gorse were produced and fully analyzed, providing a more complete and novel description, with new insights for assessing their utilization. The aims of this study were to elucidate the effects of feedstock, pyrolysis temperature, and residence time on biochar properties and to assess the adequacy of these biochars as fuel. Elemental and proximate analyses and estimations of the lower and higher heating values were performed, and physical and chemical properties, as well as several other related energy indices, were determined. The experimental results showed that the temperature was a key factor in the properties of the biochars, while residence time was less important. The BCs obtained from the two feedstocks did not show important effects on the properties, which is consistent with the fact that they are woody legumes. These biochars had a high carbon content and were thermally stable. The BCs also had a high calorific value and suitable energetic properties. Additionally, their PAH contents were low, indicating that the use of these biochars would be safe. In conclusion, broom- and gorse-derived biochars can be considered as renewable fuels for green energy production. Full article
(This article belongs to the Section Energy Science and Technology)
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17 pages, 1087 KB  
Article
Unveiling Inter- and Intra-Patient Sequence Variability with a Multi-Sample Coronavirus Target Enrichment Approach
by Sara Lado, Jakob Thannesberger, Kathrin Spettel, Jurica Arapović, Bibiana I. Ferreira, Marialuisa Lavitrano and Christoph Steininger
Viruses 2024, 16(5), 786; https://doi.org/10.3390/v16050786 - 15 May 2024
Viewed by 1784
Abstract
Amid the global challenges posed by the COVID-19 pandemic, unraveling the genomic intricacies of SARS-CoV-2 became crucial. This study explores viral evolution using an innovative high-throughput next-generation sequencing (NGS) approach. By taking advantage of nasal swab and mouthwash samples from patients who tested [...] Read more.
Amid the global challenges posed by the COVID-19 pandemic, unraveling the genomic intricacies of SARS-CoV-2 became crucial. This study explores viral evolution using an innovative high-throughput next-generation sequencing (NGS) approach. By taking advantage of nasal swab and mouthwash samples from patients who tested positive for COVID-19 across different geographical regions during sequential infection waves, our study applied a targeted enrichment protocol and pooling strategy to increase detection sensitivity. The approach was extremely efficient, yielding a large number of reads and mutations distributed across 10 distinct viral gene regions. Notably, the genes Envelope, Nucleocapsid, and Open Reading Frame 8 had the highest number of unique mutations per 1000 nucleotides, with both spike and Nucleocapsid genes showing evidence for positive selection. Focusing on the spike protein gene, crucial in virus replication and immunogenicity, our findings show a dynamic SARS-CoV-2 evolution, emphasizing the virus–host interplay. Moreover, the pooling strategy facilitated subtle sequence variability detection. Our findings painted a dynamic portrait of SARS-CoV-2 evolution, emphasizing the intricate interplay between the virus and its host populations and accentuating the importance of continuous genomic surveillance to understand viral dynamics. As SARS-CoV-2 continues to evolve, this approach proves to be a powerful, versatile, fast, and cost-efficient screening tool for unraveling emerging variants, fostering understanding of the virus’s genetic landscape. Full article
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15 pages, 808 KB  
Review
Sex Differences in Cognitive Reflection: A Meta-Analysis
by Inmaculada Otero, Alexandra Martínez, Dámaris Cuadrado, Mario Lado, Silvia Moscoso and Jesús F. Salgado
J. Intell. 2024, 12(4), 39; https://doi.org/10.3390/jintelligence12040039 - 29 Mar 2024
Cited by 4 | Viewed by 4565
Abstract
The current study presents a meta-analytic review of the differences between men and women in cognitive reflection (CR). The study also explores whether the type of CR test (i.e., numerical tests and verbal tests) moderates the relationship between CR and sex. The results [...] Read more.
The current study presents a meta-analytic review of the differences between men and women in cognitive reflection (CR). The study also explores whether the type of CR test (i.e., numerical tests and verbal tests) moderates the relationship between CR and sex. The results showed that men score higher than women on CR, although the magnitude of these differences was small. We also found out that the type of CR test moderates the sex differences in CR, especially in the numerical tests. In addition, the results showed that the length of numerical tests (i.e., number of items) does not affect the differences between men and women in CR. Finally, the implications of these results are discussed, and future research is suggested. Full article
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