Search Results (91)

Microplastics (MPs) have emerged as a growing environmental and food safety concern, with their presence widely reported in aquatic organisms and seafood. However, their occurrence in traditionally processed and fermented fish products remains unexplored. This study provides the first evidence of MP contamination in ethnic fermented fish products of Northeast India, namely Ngari, Hentak, and Shidal. MPs were analyzed for abundance, size distribution, morphology, color, and polymer composition using microscopic examination and Laser Raman Spectroscopy. The average MP abundance was 16.50 ± 5.18 MPs/g in Ngari, 15.73 ± 4.83 MPs/g in Shidal, and 20.50 ± 3.00 MPs/g in Hentak. Fibers and fragments were the dominant morphotypes across all products, with transparent and black particles occurring most frequently. Polymer characterization revealed polyethylene (PE) and polypropylene (PP) as the predominant polymers, followed by polyamide (PA), polyvinyl chloride (PVC), and polystyrene (PS). Size distribution analysis showed that MPs in the 101–300 µm range were most abundant in Ngari and Shidal, whereas smaller MPs (<50 µm) predominated in Hentak. The use of whole fish, including the gastrointestinal tract and gills, primary sites for MP accumulation, along with non-standardized fermentation practices and atmospheric deposition during retail, likely contributes to contamination. These findings highlight an overlooked route of human exposure to MPs through traditional fermented foods and underscore the need for improved processing practices and mitigation strategies to safeguard food safety and sustainability. Full article

Background/Objectives: Phthalates are encountered in everyday consumer and indoor environments, and their metabolites are commonly detected in urine. Although phthalate exposure has been linked to diabetes mellitus (DM), associations may vary by life stage. Therefore, we evaluated age-specific association between urinary phthalate metabolites and DM using nationally representative Korean data. Methods: We conducted a cross-sectional analysis of the Korean National Environmental Health Survey Cycle 4 (2018–2020). Adults aged ≥19 years with complete data were included. Eight urinary metabolites were evaluated. Metabolites were log-transformed, and those showing interaction were analyzed by tertiles. Complex survey-weighted logistic regression estimated odds ratios (95% confidence intervals) for DM, adjusting for demographic, socioeconomic, and health behavior factors. Analyses were stratified by age group. Results: Geometric mean (GM) concentrations among participants with DM varied significantly by age groups for several metabolites. Interaction analyses identified statistically significant effects for selected phthalate metabolites, including MnBP, MCPP, and MEP. In the age-stratified adjusted models, MnBP and MCPP were more strongly associated with DM in young adults, whereas the pattern for MEP appeared more evident in older adults, suggesting potential life-course differences in metabolic vulnerability. Conclusions: Associations between urinary phthalate metabolites and DM vary substantially by age, indicating life-course differences in exposure pathways and metabolic vulnerability. Age-specific prevention and surveillance strategies may improve environmental health interventions for DM. Full article

The growing demand for robust food authentication methods has driven the establishment of fast, sensitive, and field-based detection systems for identifying meat species. This study presents a colorimetric-based PSR approach for identifying yak (Bos grunniens) meat within fresh, thermally processed, and blended meat samples. Targeting the mitochondrial D-loop locus, the assay incorporates a simple alkaline lysis (AL) procedure for efficient DNA extraction, eliminating the requirement for specialized instrumentation. The PSR assay demonstrated high specificity, showing no evidence of cross-reactivity with closely associated food animals such as buffalo, cattle, goat, sheep, mithun, and pig. Sensitivity assessment revealed the assay’s capability to detect 1 pg of yak DNA, with reliable performance in samples exposed to thermal conditions up to 121 °C. Additionally, the technique detected yak meat down to a concentration of 0.1% in binary beef mixtures. This method provides a significant improvement in sensitivity over end-point PCR and is particularly well-suited for field applications due to its practical simplicity, affordability, as well as no reliance on sophisticated instrument. This is, to the best of our understanding, the first reported PSR-based approach developed for the identification of yak meat, offering a robust tool for food origin verification, regulatory enforcement, and product integrity monitoring. Full article

The Northeastern Hunan Province (NEH), situated within the Central Jiangnan Orogen, hosts abundant Au-polymetallic deposits. However, the coupling mechanism between the deep tectono-magmatism and Au-polymetallic mineralization remains poorly understood. In this study, a three-dimensional (3D) resistivity model derived from 59 magnetotelluric (MT) stations is presented to investigate the lithospheric architecture and its relationship to Au-polymetallic mineralization. The model reveals three prominent mid-to-lower crustal conductors (3–30 Ω·m) at 15–35 km depth beneath Au-polymetallic deposits along NE faults. These anomalies are interpreted as source zones and pathways for magmatic-hydrothermal fluids during the Late Mesozoic tectono-magmatism, likely formed by the enrichment of graphite films and sulfides along faults, which thus account for the observed conductive features. Moreover, the model reveals a thinning electrical lithosphere–asthenosphere boundary (eLAB) at ~80 km depth beneath the Southeastern NEH, attributed to lithospheric delamination triggered by the rollback of the Paleo-Pacific plate. This delamination facilitated the upwelling and lateral migration of asthenospheric materials, which promoted intense extension and crust–mantle interaction. Consequently, metallic elements were extensively extracted from the crust and concentrated into large-scale Au-polymetallic deposits in the NEH. Integrating with previous geochemical study, a deep-seated magmatic underplating and MASH model is proposed as key drivers of Au-polymetallic enrichment in the NEH, effectively linking deep tectono-magmatism with shallow mineralization. From a rheological perspective, three low-viscosity zones within the mid-to-lower crust likely acted as both vertical conduits and deep sources for metallogenic fluids, providing favorable pathways for their migration and accumulation. Full article

Background/Objectives: This study evaluated associations between urinary 3-phenoxybenzoic acid (3-PBA), a metabolite of pyrethroids, and cardiometabolic indicators in a nationally representative sample of Korean adults using data from the 2nd Korean National Environmental Health Survey (KoNEHS 2012–2014). Methods: Urinary 3-PBA concentrations were creatinine-adjusted; participants with urinary creatinine < 0.3 or > 3.0 g/L were excluded. Associations with triglycerides, BMI, HDL cholesterol, TSH, and T4 were analyzed using non-parametric tests and multiple regression, with additional verification through log-transformed variables and multiple-comparison control. Results: Urinary 3-PBA levels were higher in females, increased with age, and were elevated among rural residents and frequent pesticide users. Triglycerides and TSH showed positive associations with 3-PBA, whereas T4 showed a negative association. BMI displayed a weak negative correlation without consistent significance, and HDL cholesterol was not statistically significant. In multiple regression models, triglycerides, TSH, and T4 remained significantly associated with urinary 3-PBA. Conclusions: Statistically significant associations were observed between urinary 3-PBA concentrations and several cardiometabolic indicators, including triglycerides, TSH, and T4, in Korean adults. These findings suggest that even low-level environmental exposure to pyrethroids may influence lipid metabolism and thyroid function. Given the cross-sectional design and the short biological half-life of 3-PBA, the results should be interpreted as associations rather than causation, highlighting the need for longitudinal studies and continued biomonitoring. Full article

Although several articles and books on the prophecy of women and LGBTIQ* persons in the Ancient Near East and the Old Testament have been published in recent decades, their outcomes have scarcely been received in traditional research on prophecy. This article will deal with female prophecy, traces of which we find in all parts of the canon: the Torah (Miriam), the Nebiim Rishonim (Deborah and Huldah, the first and last in the succession of Moses), in the prophetic books (the nameless prophetess to whom Isaiah goes in Isa 8, as well as the prophetic daughters in Joel 3), and also in the Ketubim (Noadiah in Neh 6 and the versions of the Chronicler). However, there are also false female prophets like the Women of En Dor (1 Sam 28) and the prophesying daughters in Ezek 13. Prophetic women such as Noadiah, and those in Ex 38:8 and 1 Sam 2:22, and, in the same tradition, Hannah in the New Testament, are also present in cultic places. Additionally, women are also found among the group gathered around a central prophetic figure, such as the women of Shunem. This article, on the one hand, reveals the gender bias in traditional semantic and grammatical analysis, and on the other hand, will show the importance of the stylistic features of the few texts under discussion (e.g., inclusions, exposed positions in compositions), which may provide illuminating conclusions for the whole phenomenon of prophecy. Full article

Background/Objectives: In this study, we conducted molecular identification of R.microplus and explored the genetic diversity of R. microplus for the first time in Mizoram, a Northeastern Hill (NEH) state of India bordering Myanmar. Methods: To assess genetic variation and evolutionary relationships, we employed phylogenetic analyses, genetic divergence metrics, and haplotype network construction based on mitochondrial (COX1 and 16S rDNA) and nuclear (ITS-2 and 18S rDNA) markers. Additionally, multivariate Principal Coordinate Analysis (PCoA) was used to visualize genetic differentiation among R. microplus populations. Results: Our analyses indicated that populations of R. microplus sensu lato from India, Bangladesh, and Pakistan form a closely related matrilineal lineage distinct from R. microplus sensu stricto, clustering within clade C of the COX1-based phylogeny. Globally, 24 COX1 haplotypes were recovered, with 1 haplotype identified in India. The Mizoram population exhibited a single 16S rDNA haplotype; however, intraspecific divergence was evident across India, with seven matrilineal haplotypes detected and nineteen globally. Further, five haplotypes were identified within R. microplus using the ITS-2 marker, while five haplotypes were observed within the Rhipicephalus genus using the 18S rDNA marker. Moreover, this study revealed the presence of Coxiella-like endosymbionts in 95% of the tick specimens analyzed. Conclusions: This study fills a critical knowledge gap by providing the first molecular documentation of tick diversity in Mizoram, a strategic region along the Indo–Myanmar border, and offers novel insights into the phylogeography and symbiotic associations of R. microplus and related tick taxa. Full article

The Distributed No-Wait Flow Shop Scheduling Problem (DNWFSP) arises in various manufacturing contexts, such as chemical production and electronic assembly, where strict no-wait constraints and multi-factory coordination are required. Solving the DNWFSP involves determining the allocation of jobs to factories and the no-wait processing sequences within each factory, making it a highly complex combinatorial problem. To address the limitations of existing methods—including poor initial solution quality, limited neighborhood exploration, and a tendency to converge prematurely—this paper proposes a Multi-Neighborhood Adaptive Tabu Search Algorithm (MNATS). The MNATS integrates a balance–lookahead NEH initializer (BL-NEH), an adaptive neighborhood local search (ANLS) strategy, and an Adaptive Tabu-Guided Perturbation (ATP) strategy. Experimental results on multiple benchmark instances demonstrate that MNATS algorithm significantly outperforms several state-of-the-art algorithms in terms of solution quality and robustness. Full article

Efficient production scheduling is a key challenge in industrial operations and continues to attract significant interest within the field of operations research. This paper investigates a range of methodological approaches designed to solve the permutation flow shop scheduling problem (PFSP) with sequence-dependent setup times (SDST). The main objective is to minimize the total weighted flow time (TWFT) while ensuring a no-wait production environment. The proposed solution strategy is based on using algorithms with a mixed integer linear programming (MILP) formulation, heuristics, and their combination. The heuristics utilized in this paper include an advanced greedy randomized adaptive search procedure (GRASP) based on a priority rule and Hybrid-GRASP-NEH (HGRASP), where Nawaz-Enscore-Ham (NEH) takes place to initiate solutions, based on iterative global and local search methods to refine exploration capabilities and improve solution quality. These approaches were validated using a comprehensive set of experiments across diverse instance sizes that proved the efficiency of HGRASP, with the results showing a high-performance level that closely matched that of the exact MILP approach. Statistical analysis via the Friedman test (χ² = 46.75, p = 7.04 × 10⁻¹¹) confirmed significant performance differences among MILP, GRASP, and HGRASP. While MILP guarantees theoretical optimality, its practical effectiveness was limited by imposed computational time constraints, and HGRASP consistently achieved near-optimal solutions with superior computational efficiency, as demonstrated across diverse instance sizes. Full article

Distributed permutation flowshop scheduling is an NP-hard problem that has become a hot research topic in the fields of optimization and manufacturing in recent years. Multimodal optimization finds multiple global and local optimal solutions of a function. This study proposes a harmony search algorithm with iterative optimization operators to solve the NP-hard problem for multimodal optimization with the objective of makespan minimization. First, the initial solution set is constructed by using a distributed NEH operator. Second, after generating new candidate solutions, efficient iterative optimization operations are applied to optimize these solutions, and the worst solutions in the harmony memory (HM) are replaced. Finally, the solutions that satisfy multimodal optimization of the harmony memory are obtained when the stopping condition of the algorithm is met. The constructed algorithm is compared with three meta-heuristics: the iterative greedy meta-heuristic algorithm with a bounded search strategy, the improved Jaya algorithm, and the novel evolutionary algorithm, on 600 newly generated datasets. The results show that the proposed method outperforms the three compared algorithms and is applicable to solving distributed permutation flowshop scheduling problems in practice. Full article

This study investigated the effects of dietary Gonadotropin-releasing hormone (GnRH) and domperidone on the reproductive performance of Devario devario during a 40-day trial. Five treatment groups received varying doses of GnRH (100, 50, 25, 12.5 µg/kg body weight) in combination with domperidone (50, 25, 12.5, 6.25 mg/kg body weight), embossed in a gel-based diet alongside a control group without the exogenous hormones. Reproductive performance was examined by measuring the gonadosomatic index, fecundity, reproductive hormone levels, and histological features of the gonads, blood parameters, and antioxidant enzyme activity. The T1 group (100 µg GnRH + 50 mg domperidone) exhibited the highest GSI in both sexes. The histological analysis of testes from T1, T2 (50 µg GnRH + 25 mg domperidone), and T3 (25 µg GnRH + 12.5 mg domperidone) groups revealed an increased presence of late-stage spermatids and spermatozoa. In females, the T2 group produced the highest proportion of advanced-stage oocytes and demonstrated the greatest absolute fecundity (1300 ± 23 eggs). However, the control group showed the highest fertilization and hatching rates. Testosterone levels were significantly elevated in the T3 group, while vitellogenin levels increased in the T1 and T2 groups. Antioxidant enzyme activity varied, with the T1 group displaying higher superoxide dismutase activity in gills and liver, and the T2 group showing increased SOD activity in muscle and brain. Improvements in haematological parameters were observed across all treatments. These results suggest that an optimal dose of 50 µg GnRH + 25 mg domperidone can enhance reproductive performance in D. devario. Full article

Oxidative stress poses a challenge to in vitro embryo culture. As a flavonoid, galangin (GAL) has been shown to have antioxidant effects, but the effect and antioxidant capacity of GAL in the in vitro development of porcine parthenogenetic embryos are still unknown. In this study, we demonstrated that 1 µM GAL significantly increased the blastocyst rate, decreased the accumulation of intracellular reactive oxygen species (ROS), increased the glutathione (GSH) level, and enhanced mitochondrial function in early porcine embryos. Nuclear factor erythroid-2-related factor 2 (Nrf2) was identified as the target gene of GAL via network pharmacology, and the transcript levels of related antioxidant enzymes (HO-1, NQO1, SOD2, and CAT) were found to be increased. Since Nrf2 has seven domains, we constructed Nrf2 mutants lacking different domains in vitro. We found that GAL specifically binds to the Neh1 domain of Nrf2. Subsequent embryonic experiments demonstrated that the antioxidant effect of GAL was abolished after Nrf2 deletion. These results suggest that GAL can directly bind to Nrf2 to regulate the level of oxidative stress and improve mitochondrial function in embryos. Full article

Background: Environmental tobacco smoke (ETS) exposes adolescents to various environmental toxins, potentially affecting their developmental health. However, limited research exists on the associations between ETS exposure and the bodily burdens of environmental chemicals on adolescents. This study aimed to investigate the relationship between ETS exposure and the concentration of various environmental chemicals in adolescents, utilizing urinary cotinine as an objective biomarker. Methods: Data from 828 adolescents aged 12–17 years participating in the Korean National Environmental Health Survey (KoNEHS) were analyzed. ETS exposure was assessed via self-reported questionnaires and confirmed by urinary cotinine measurements. Levels of 33 environmental chemicals, including heavy metals, polycyclic aromatic hydrocarbons (PAHs), phthalates, phenols, volatile organic compounds (VOCs), and per- and polyfluoroalkyl substances (PFASs), were measured. Statistical analyses were conducted after adjusting for covariates. Results: Adolescents exposed to ETS showed significantly higher urinary cotinine and mono-(2-ethyl-5-carboxypentyl) phthalate (MECPP) concentrations than non-exposed adolescents. Additionally, significant positive correlations were observed between urinary cotinine levels and metabolites of PAHs (NAP, OHFlu), phenols (BPA, BPS), phthalates (MMP), and VOCs (t,t-MA) after adjustments. However, ETS exposure was not significantly associated with heavy metal concentrations. Conclusions: This study described the association between ETS exposure and environmental chemicals. A trend has been identified between ETS exposure in adolescents and increased bodily concentrations of various environmental chemicals, including PAHs, phenols, phthalates, and VOCs. As adolescence is a critical developmental period of vulnerability to environmental toxins, reducing ETS exposure to protect adolescents’ health and prevent potential lifelong health effects should be emphasized. This study was based on a cross-sectional design, and some confounding factors and measurement limitations may exist. Therefore, caution is needed in interpreting causality, and further research is recommended to determine more precise causality and long-term health effects. Full article

In order to address the Distributed Displacement Flow Shop Scheduling Problem (DPFSP) with uncertain processing times in real production environments, Plant Simulation is employed to construct a simulation model for the MSRDPFSP. The model conducts quantitative analyses of workshop layout, assembly line design, worker status, operating status of robotic arms and AGV vehicles, and production system failure rates. A hybrid NEH-DDQN algorithm is integrated into the simulation model via a COM interface and DLL, where the NEH algorithm ensures the model maintains optimal performance during the early training phase. Four scheduling strategies are designed for workpiece allocation across different workshops. A deep neural network replaces the traditional Q-table for greedy selection among these four scheduling strategies, using each workshop’s completion time as a simplified state variable. This approach reduces algorithm training complexity by abstracting away intricate workpiece allocation details. Experimental comparisons show that for the data of 500 workpieces, the NEH algorithm in 3 s demonstrates equivalent quality to that produced by the GA algorithm in 300 s. After 2000 iterations, the DDQN algorithm achieves a 15% reduction in makespan with only a 2.5% increase in computational time compared to random search, this joint simulation system offers an efficient and stable solution for the modeling and optimization of the MSRDPFSP issue. Full article

Passion fruit is an underutilised fruit in Northeastern India, known for its unique flavour and health benefits. This study analysed 15 genotypes (P1 to P15) to explore their morphological and biochemical traits related to fruit quality and yield. P. quadrangularis L. (P15) exhibited maximum flower length, fruit size, weight, juice content, shelf-life, and yield. P. edulis f. flavicarpa (P3, P5, and P2) had the highest seed count per fruit and antioxidant activity, along with greater chlorophyll and anthocyanin levels. Passiflora edulis Sims (P8 and P11) showed superior total soluble solids, carotenoids, and vitamin C. The study found that fruit shelf life positively correlated with seed weight, while the number of fruits per vine negatively correlated with seed traits and peel weight. Additionally, certain traits, such as total carotenoids, had strong positive correlations with reducing sugar and flavonoids. Principal component analysis revealed distinct trait relationships, particularly for genotypes P7 and P10. SDS-PAGE protein profiling indicated a significant distance between P3 and P14, emphasising genetic diversity. In conclusion, this research highlights the diverse morphological and biochemical characteristics of passion fruit genotypes, paving the way for the region’s improved fruit quality, yield, and breeding strategies. Full article