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Search Results (666)

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Keywords = Pond-in-Pond system

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17 pages, 7055 KB  
Article
Studies on the Migration of Metal Ions in the Aquifer and the Seepage Prevention of Intercepting Walls in Lead–Zinc Mining Areas
by Shuangcheng Tang, Xuehai Fu, Haiyue Lin, Zexuan Liao, Baolei Xie, Zhiwen Xue, Guanyu Zhao, Wei Qiao and Qiqing Wang
Water 2025, 17(19), 2828; https://doi.org/10.3390/w17192828 - 26 Sep 2025
Abstract
As metal resource extraction increases, heavy metal ion pollution in the saturated zone intensifies. Hence, research on the migration of heavy metal ions in aquifers and the efficacy of protective measures is essential to inform pollution prevention and control engineering. This study focuses [...] Read more.
As metal resource extraction increases, heavy metal ion pollution in the saturated zone intensifies. Hence, research on the migration of heavy metal ions in aquifers and the efficacy of protective measures is essential to inform pollution prevention and control engineering. This study focuses on the slag pond and its surrounding area of a smelting plant. Utilizing field hydrological surveys and experiments, and data from previous studies, we employed FEFLOW7.0 simulation software to model the groundwater system of the boulder aquifer in this region. The model divides the domain based on natural topography: the eastern river serves as a constant-head boundary, while other areas are set as specified-flux boundaries. The impermeable layer at the bottom is treated as a no-flow boundary, with a maximum simulation period of 2500 days. The simulation examines the natural movement of zinc ions and how the construction of the wall impacts their migration, as well as the wall’s effectiveness in preventing seepage. Findings indicate that the movement of zinc ions is significantly influenced by the reaction coefficient. When the reaction coefficient exceeds 10−8 s−1, zinc ions decrease rapidly in the area. After the construction of the cutoff wall, the maximum migration distance of zinc ions within 2500 days decreased from 220 m to 77 m, demonstrating its effectiveness in controlling zinc transport in groundwater. Full article
(This article belongs to the Section Hydrogeology)
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15 pages, 478 KB  
Article
Nitrogen Budget in Yellow-Tail Lambari Monoculture and Integrated Aquaculture
by Dalton Belmudes, Andre Z. Boaratti, Paulo V. L. Mantoan, Aline M. Marques, Julia R. C. Ferreira, Patricia Moraes-Valenti, Dallas L. Flickinger and Wagner C. Valenti
Fishes 2025, 10(10), 480; https://doi.org/10.3390/fishes10100480 - 26 Sep 2025
Abstract
This study quantified nitrogen (N) inputs by water, feed, animals, and atmospheric gas and outputs by water, harvested animals, sediments, and gas emissions in earthen ponds used for the monoculture and integrated cultures of yellow-tail lambari (Astyanax lacustris), Amazon River prawn [...] Read more.
This study quantified nitrogen (N) inputs by water, feed, animals, and atmospheric gas and outputs by water, harvested animals, sediments, and gas emissions in earthen ponds used for the monoculture and integrated cultures of yellow-tail lambari (Astyanax lacustris), Amazon River prawn (Macrobrachium amazonicum), and curimbata (Prochilodus lineatus), and evaluated whether epibenthic species improve N retention in harvested biomass. Three systems with four replicates were tested, lambari monoculture (L), lambari–prawn (LP), and lambari–prawn–curimbata (LPC), stocked at 50, 25, and 13 individuals m−2, respectively. Feed N was the major input (67–75%), followed by inlet water (19–30%). Harvested biomass represented 20–23% of total outputs, sediments 25–33%, and gaseous emissions 7–29%, while outlet water contributed <3%. N lost through seepage was highest in L (70.5 ± 22.9 kg N ha−1). N2 ebullition increased with benthic species, from 10.4 ± 10.6 kg N ha−1 (L) to 72.1 ± 32.4 kg N ha−1 (LPC). N recovered in lambari was 43.2 ± 7.4 kg N ha−1 in LPC, 36 ± 8.6 in L, and 33 ± 5.6 in LP. Considering all species, recovery of dietary N increased from 20.0 ± 4.3% (L) to 35.0 ± 5.9% (LPC), and recovery from all inputs rose from 13.0 ± 2.2% to 18.0 ± 3.4%. Integrated systems, particularly LPC, enhanced N retention in biomass and reduced environmental losses. Full article
(This article belongs to the Special Issue Integrated Multi-Trophic Aquaculture (IMTA))
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48 pages, 12849 KB  
Article
Analysis of the Functional Efficiency of a Prototype Filtration System Dedicated for Natural Swimming Ponds
by Wojciech Walczak, Artur Serafin, Tadeusz Siwiec, Jacek Mielniczuk and Agnieszka Szczurowska
Water 2025, 17(19), 2816; https://doi.org/10.3390/w17192816 - 25 Sep 2025
Abstract
Water treatment systems in swimming ponds support the natural self-cleaning capabilities of water based on the functions of repository macrophytes in their regeneration zone and the regulation of the internal metabolism of the reservoirs. As part of the project, a functional modular filtration [...] Read more.
Water treatment systems in swimming ponds support the natural self-cleaning capabilities of water based on the functions of repository macrophytes in their regeneration zone and the regulation of the internal metabolism of the reservoirs. As part of the project, a functional modular filtration chamber with system multiplication capabilities was designed and created. This element is dedicated to water treatment systems in natural swimming ponds. The prototype system consisted of modular filtration chambers and pump sections, as well as equipment adapted to the conditions prevailing in the eco-pool. An innovative solution for selective shutdown of the filtration chamber without closing the circulation circuit was also used, which forms the basis of a patent application. A verified high-performance adsorbent, Rockfos® modified limestone, was used in the filtration chamber. In order to determine the effective filtration rate for three small test ponds with different flow rates (5 m/h, 10 m/h and 15 m/h), the selected physicochemical parameters of water (temperature, pH, electrolytical conductivity, oxygen saturation, total hardness, nitrites, nitrates, and total phosphorus, including adsorption efficiency and bed absorption capacity) were researched before and after filtration. Tests were also carried out on the composition of fecal bacteria and phyto- and zooplankton. Based on high effective phosphorus filtration efficiency of 32.65% during the operation of the bed, the following were determined: no exceedances of the standards for the tested parameters in relation to the German standards for eco-pools (FLL—Forschungsgesellschaft Landschaftsentwicklung Landschaftsbau e. V., 2011); lower number of fecal pathogens (on average 393—coliform bacteria; 74—Escherichia coli; 34—fecal enterococci, most probably number/100 mL); the lowest share of problematic cyanobacteria in phytoplankton (<250,000 individuals/dm3 in number and <0.05 µg/dm3—biomass); low chlorophyll a content (2.2 µg/dm3—oligotrophy) and the presence of more favorable smaller forms of zooplankton, an effective filtration speed of 5 m/h. This velocity was recommended in the FLL standards for swimming ponds, which were adopted in this study as a reference for rapid filters. In testing the functional efficiency of a dedicated filtration system for a Type II test pond (50 m2—area and 33 m3—capacity), at a filtration rate of 5 m/h, an average effective phosphorus adsorption efficiency of 18.28–53.98% was observed under the bed work-in-progress conditions. Analyses of other physicochemical water parameters, with appropriate calculations and statistical tests, indicated progressive functional efficiency of the system under bathing conditions. Full article
(This article belongs to the Section Water Quality and Contamination)
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16 pages, 4760 KB  
Article
The Stone Moroko Pseudorasbora parva Altered the Composition and Stability of Sediment Microbial Communities Within the Chinese Mitten Crab (Eriocheir sinensis) Polyculture Pond
by Yiran Hou, Yun Bao, Rui Jia, Linjun Zhou, Lili Song, Baojuan Yang, Bing Li and Jian Zhu
Biology 2025, 14(9), 1297; https://doi.org/10.3390/biology14091297 - 19 Sep 2025
Viewed by 297
Abstract
Integrated aquaculture, centered around polyculture involving multiple species, is a typical practice for the sustainable development of the aquaculture industry, capable of enhancing resource utilization efficiency, environmental stability, and overall productivity through establishing symbiotic interactions among species. This study employed multi-amplicon high-throughput sequencing [...] Read more.
Integrated aquaculture, centered around polyculture involving multiple species, is a typical practice for the sustainable development of the aquaculture industry, capable of enhancing resource utilization efficiency, environmental stability, and overall productivity through establishing symbiotic interactions among species. This study employed multi-amplicon high-throughput sequencing to assess the ecological impacts of two polyculture methods involving river crabs on sediment bacteria, fungi, and protists. One method involved polyculturing river crabs with mandarin fish, silver carp, and the stone moroko (SPC), and the other involved polyculturing river crabs with only mandarin fish and silver carp (SMC). The results showed that, compared to the SMC group, the SPC group remarkably increased the Chao1 index of bacterial communities in pond sediment and decreased the Pielou_J index of protists. The relative abundances of all fungal phyla and most dominant bacterial and protistan phyla (top 10 in relative abundance) in the SPC group were considerably different from those in the SMC group. In the co-occurrence networks of bacterial, fungal, and protistan communities, the numbers of edges and nodes were higher in the SPC group than in the SMC group, and the habitat niche breadth of bacterial community was also notably increased in the SPC group. The levels of total carbon (TC), total nitrogen (TN), and phosphates within pond sediment in the SPC group were obviously lower than those in the SMC group, and were significantly correlated with the microbial communities, with TC being identified as the primary contributor driving changes in the microbial communities. All the findings collectively demonstrate that the polyculture of river crabs with mandarin fish, silver carp, and the stone moroko enhances the stability of bacterial, fungal, and protistan communities in sediment and enhances resource utilization efficiency in aquaculture, thereby preventing the environmental risks associated with excessive nutrient accumulation in sediment. Polyculture systems integrating river crabs with mandarin fish, silver carp, and the stone moroko represent a sustainable aquaculture model with significant ecological benefits. Full article
(This article belongs to the Special Issue Biodiversity, Conservation, and Application of Crustaceans)
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19 pages, 4083 KB  
Article
Design and Analysis of a Dual-Screw Propelled Robot for Underwater and Muddy Substrate Operations in Agricultural Ponds
by Yan Xu, Pengchao Dai, Mingjin Xin, Liyan Wu and Yuqiu Song
Actuators 2025, 14(9), 450; https://doi.org/10.3390/act14090450 - 12 Sep 2025
Viewed by 408
Abstract
Conventional underwater vehicles, which are typically equipped with oscillating fins or standard propellers, are incapable of effective locomotion within the viscous, high-resistance environment of muddy substrates common in agricultural ponds. To address this operational limitation, this paper presents a compact dual-screw propelled robot [...] Read more.
Conventional underwater vehicles, which are typically equipped with oscillating fins or standard propellers, are incapable of effective locomotion within the viscous, high-resistance environment of muddy substrates common in agricultural ponds. To address this operational limitation, this paper presents a compact dual-screw propelled robot capable of traversing both the water column and soft substrate layers. The robot’s locomotion is driven by two optimized helical screw propellers, while depth control and roll stability are actively managed by a control fin. A dynamic model of the robot–fluid interaction was developed to optimize the screw configuration that achieves a maximum theoretical thrust of 40 N with a calculated 16% slippage rate in mud. Computational fluid dynamics simulations were employed to determine the optimal angle for the control fin, which was found to be 9°, maximizing the lift-to-drag ratio at 12.09 for efficient depth maneuvering. A cable-free remote control system with a response time of less than 0.5 s governs all operations. Experimental validation in a controlled tank environment confirmed the robot’s performance, demonstrating stable locomotion at 0.4 m/s in water and 0.3 m/s in a simulated mud substrate. This dual-screw propelled robot represents a promising technological solution for comprehensive monitoring and operational tasks in agricultural pond environments. Full article
(This article belongs to the Special Issue Design and Control of Agricultural Robotics)
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15 pages, 4334 KB  
Article
Transcriptome Analyses of Procambarus clarkii (Girard, 1852) Under Individual Exposures to CuSO4, Pendimethalin, and Glyphosate
by Yao Zheng, Jiajia Li, Zhuping Liu, Ning Wang and Gangchun Xu
Toxics 2025, 13(9), 765; https://doi.org/10.3390/toxics13090765 - 9 Sep 2025
Viewed by 527
Abstract
Pesticide usage in the integrated rice–crayfish system has aroused lots of attention all over the world. Especially in China, fish farmers often use copper sulfate and pendimethalin to remove moss from aquaculture water and glyphosate to remove weeds in and around crayfish–crab mixed [...] Read more.
Pesticide usage in the integrated rice–crayfish system has aroused lots of attention all over the world. Especially in China, fish farmers often use copper sulfate and pendimethalin to remove moss from aquaculture water and glyphosate to remove weeds in and around crayfish–crab mixed culture ponds. To explore the stress response mechanism of CuSO4, pendimethalin, and glyphosate to the hepatopancreas of Procambarus clarkii (Girard, 1852), seven treatment groups including control, CuSO4 (1 and 2 mg·L−1), pendimethalin (PND, 5 and 10 μg·L−1), and glyphosate (5 and 10 μg·L−1) experimental groups were set up; the transcriptome responses were detected at 4, 8, and 12 days, respectively. The irregular structure and vacuoles were shown in the hepatopancreas for 2 mg·L−1 CuSO4 and 10 μg·L−1 glyphosate exposures at 12 d, while narrowed hepatic sinusoids were revealed after 10 μg·L−1 pendimethalin exposure. The pathways of ribosome, lysosome, and peroxisome were significantly enriched for differential expression genes (DEGs); in addition, tyrosine metabolism, starch, and sucrose metabolism were enriched under the stress of the three inputs. Genes in related pathways such as glycerophospholipid metabolism, oxidative phosphorylation, and glycerolipid metabolism also changed, and the expression of genes associated with oxidative phosphorylation changed significantly under the stress of the three inputs. Oxidative stress, neurotoxicity, metabolism, and energy supply have been significantly affected by the above herbicide exposure. High concentrations and/or long-term duration exposure may result in metabolic disorders rather than eliminate toxicity through adaptability responses. Full article
(This article belongs to the Section Ecotoxicology)
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13 pages, 2040 KB  
Article
Microstructures as Models for Origin of Life in Hot Water: Hydrogen-Assisted Self-Assembly of Glycine and Alanine Zwitterions
by Ignat Ignatov
Hydrogen 2025, 6(3), 67; https://doi.org/10.3390/hydrogen6030067 - 9 Sep 2025
Viewed by 404
Abstract
Building on the early investigation by Sidney W. Fox that dry-heated amino acids can spontaneously form microspheres, this research studies the self-organization of glycine and alanine with hydrogen in a liquid system. This study aimed to investigate the spontaneous formation of membraneless, microscale [...] Read more.
Building on the early investigation by Sidney W. Fox that dry-heated amino acids can spontaneously form microspheres, this research studies the self-organization of glycine and alanine with hydrogen in a liquid system. This study aimed to investigate the spontaneous formation of membraneless, microscale amino acid assemblies under simulated prebiotic hydrothermal conditions, such as hot mineral sources and ponds. Aqueous solutions of glycine and alanine were prepared in a hydrogen-rich mineral buffer and thermally incubated at 75 °C. Phase-contrast microscopy, transmission electron microscopy (TEM), and molecular modeling were employed to analyze the morphology and internal organization of the resulting structures. Microscopy revealed that zwitterionic glycine and alanine spontaneously self-organize into spherical microspheres (~12 µm), in which the charged –NH3+ and –COO groups orient outward, while the hydrophobic methyl groups of alanine point inward, forming a stabilized internal core. The primary studies were performed with hot mineral water from Rupite, Bulgaria, at 73.4 °C. The resulting osmotic pressure difference Δπ ≈ 2490 Pa, derived from the van’t Hoff equalization. This suggests a chemically asymmetric system capable of sustaining directional water flux and passive molecular enrichment. The zwitterionic nature of glycine and alanine, which possesses both –NH3+ and –COO groups, supports the formation of microspheres in our experiments. Under conditions with hot mineral water and hydrogen acting as a reducing agent in the primordial atmosphere, these amino acids self-organized into dense interfacial microspheres. These findings support the idea that thermally driven, zwitterion-mediated aggregation of simple amino acids, such as glycine and alanine, with added hydrogen, could generate membraneless, selectively organized microenvironments on the early Earth. Such microspheres may represent a plausible intermediate between dispersed organisms and microspheres. Full article
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20 pages, 618 KB  
Review
Analysis of Edaphic Factors on the Role of Probiotics in the Development of Sustainable and Productive Aquaculture
by Dmitry Rudoy, Besarion Meskhi, Anastasiya Olshevskaya, Denis Kozyrev, Victoria Shevchenko, Mary Odabashyan, Svetlana Teplyakova and Alexander Rybak
Fishes 2025, 10(9), 457; https://doi.org/10.3390/fishes10090457 - 8 Sep 2025
Viewed by 651
Abstract
The use of antibiotics in aquaculture is associated with significant environmental risks, including ecosystem disruption and the accumulation of antibiotics in reservoirs and soil cover, as well as the spread of antibiotic-resistant strains, which encourages the search for sustainable alternatives, such as probiotics. [...] Read more.
The use of antibiotics in aquaculture is associated with significant environmental risks, including ecosystem disruption and the accumulation of antibiotics in reservoirs and soil cover, as well as the spread of antibiotic-resistant strains, which encourages the search for sustainable alternatives, such as probiotics. This review summarizes the research results on the use of probiotics in aquaculture systems. Special attention is paid to the action mechanisms and diverse effects on the health of aquatic animals, water quality and, most importantly, on the properties of soil in ponds. The research results show that certain strains of probiotics, in particular Bacillus spp., effectively decompose organic substances in sediments, reduce toxic metabolites’ concentration (ammonia, nitrites, hydrogen sulfide), stabilize soil structure, improve aeration and regulate sediments’ pH level and microbial diversity. However, the efficacy in field conditions can vary. Probiotics represent a science-based strategy to reduce dependence on antibiotics, increase system resilience by improving soil and water conditions, and increase productivity. In order to achieve maximum results, it is necessary to optimize the application methods, whilst taking into account local environmental factors. Full article
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26 pages, 6536 KB  
Article
Biogenic CO2, CH4, and N2O Emissions from Abalone Culture in Tidal Ponds
by Yi-Jung Chen, Wen-Chen Chou, Hsiao-Chun Tseng, Ruei-Feng Shiu, Meng-Chou Lee, Fan-Hua Nan and Han-Yang Yeh
Environments 2025, 12(9), 313; https://doi.org/10.3390/environments12090313 - 4 Sep 2025
Cited by 1 | Viewed by 756
Abstract
Abalone is among the most highly prized seafoods, valued for its delicate flavor and texture. As abalone aquaculture continues to expand, addressing its environmental impacts has become increasingly important. Although aquaculture is recognized as a contributor to greenhouse gas (GHG) emissions, the specific [...] Read more.
Abalone is among the most highly prized seafoods, valued for its delicate flavor and texture. As abalone aquaculture continues to expand, addressing its environmental impacts has become increasingly important. Although aquaculture is recognized as a contributor to greenhouse gas (GHG) emissions, the specific mechanisms and pathways of GHG emissions—particularly in abalone farming—remain poorly understood. To clarify the patterns and drivers of GHG emissions in abalone (Haliotis discus) culture systems, this study was conducted in three aquaculture ponds located in Gongliao District, New Taipei City, Taiwan. We measured CO2, CH4, and N2O fluxes along with key environmental parameters to assess variation across sampling locations, times, and seasons. The results showed that sampling time had no significant effect on GHG flux variations, whereas seasonal changes influenced all three gases, and sampling location significantly affected N2O flux only. During the culture period, average fluxes were 2.19 ± 10.83 mmol m−2 day−1 for CO2, 2.11 ± 2.81 µmol m−2 day−1 for CH4, and 1.65 ± 2.73 µmol m−2 day−1 for N2O, indicating that the abalone ponds served as net sources of these GHGs. When converted to CO2-equivalents (CO2-eq), the total average CO2-eq flux from the ponds was 0.02 ± 0.09 mg CO2-eq m−2 day−1, calculated using global warming potential (GWP20 and GWP100) metrics. This study provides the first comprehensive assessment of GHG emissions in abalone pond systems and offers valuable insights into their emission dynamics. The findings contribute to the scientific basis needed to improve aquaculture GHG inventories. Full article
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29 pages, 5574 KB  
Article
Comprehensive Fish Feeding Management in Pond Aquaculture Based on Fish Feeding Behavior Analysis Using a Vision Language Model
by Divas Karimanzira
Aquac. J. 2025, 5(3), 15; https://doi.org/10.3390/aquacj5030015 - 3 Sep 2025
Viewed by 548
Abstract
For aquaculture systems, maximizing feed efficiency is a major challenge since it directly affects growth rates and economic sustainability. Feed is one of the largest costs in aquaculture, and feed waste is a significant environmental issue that requires effective management strategies. This paper [...] Read more.
For aquaculture systems, maximizing feed efficiency is a major challenge since it directly affects growth rates and economic sustainability. Feed is one of the largest costs in aquaculture, and feed waste is a significant environmental issue that requires effective management strategies. This paper suggests a novel approach for optimal fish feeding in pond aquaculture systems that integrates vision language models (VLMs), optical flow, and advanced image processing techniques to enhance feed management strategies. The system allows for the precise assessment of fish needs in connection to their feeding habits by integrating real-time data on biomass estimates and water quality conditions. By combining these data sources, the system makes informed decisions about when to activate automated feeders, optimizing feed distribution and cutting waste. A case study was conducted at a profit-driven tilapia farm where the system had been operational for over half a year. The results indicate significant improvements in feed conversion ratios (FCR) and a 28% reduction in feed waste. Our study found that, under controlled conditions, an average of 135 kg of feed was saved daily, resulting in a cost savings of approximately $1800 over the course of the study. The VLM-based fish feeding behavior recognition system proved effective in recognizing a range of feeding behaviors within a complex dataset in a series of tests conducted in a controlled pond aquaculture setting, with an F1-score of 0.95, accuracy of 92%, precision of 0.90, and recall of 0.85. Because it offers a scalable framework for enhancing aquaculture resource use and promoting sustainable practices, this study has significant implications. Our study demonstrates how combining language models and image processing could transform feeding practices, ultimately improving aquaculture’s environmental stewardship and profitability. Full article
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18 pages, 1976 KB  
Article
Diversity Analysis of Microbial Communities in Shrimp Polyculture Ponds in Coastal Saline–Alkali Regions of Hebei, China
by Enhui Suo, Zhao Chen, Huan Gao, Shijia Yuan and Zhiqiang Chang
Fishes 2025, 10(9), 433; https://doi.org/10.3390/fishes10090433 - 2 Sep 2025
Viewed by 314
Abstract
To investigate the structure and successional dynamics of microbial communities in shrimp culture ponds in coastal saline–alkali regions of Hebei, China, we compared the water microbiota of Litopenaeus vannamei monoculture ponds and L. vannameiMacrobrachium rosenbergii polyculture ponds in the early, mid, [...] Read more.
To investigate the structure and successional dynamics of microbial communities in shrimp culture ponds in coastal saline–alkali regions of Hebei, China, we compared the water microbiota of Litopenaeus vannamei monoculture ponds and L. vannameiMacrobrachium rosenbergii polyculture ponds in the early, mid, and late culture stages. The results revealed clear temporal succession patterns in both the diversity and composition of microeukaryotic and bacterial communities. Distinct differences were also observed between the two culture models. Compared with monoculture, polyculture ponds showed 2.23–34.76% lower abundances of parasitic microeukaryotes, such as Rozellomycota and Perkinsida. In contrast, the abundances of carbon- and nitrogen-cycling bacterial groups (e.g., LD29, CL500-29_marine_group) and Chlorophyta were 0.24–50.94% higher in the polyculture system. Co-occurrence network analysis showed that polyculture enhanced competitive interactions and increased the network structural complexity within bacterial and cross-domain microbial networks. These findings help elucidate the mechanisms underlying efficient shrimp production in saline–alkali ponds and support the optimization of aquaculture models. Full article
(This article belongs to the Section Sustainable Aquaculture)
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21 pages, 12309 KB  
Article
Analysis of Surface Runoff and Ponding Infiltration Patterns Induced by Underground Block Caving Mining—A Case Study
by Shihui Jiao, Yong Zhao, Tianhong Yang, Xin Wen, Qingshan Ma, Qianbai Zhao and Honglei Liu
Appl. Sci. 2025, 15(17), 9516; https://doi.org/10.3390/app15179516 - 29 Aug 2025
Viewed by 358
Abstract
Surface subsidence induced by underground mining in mining areas significantly alters surface topography and hydrogeological conditions, forming depressions and fissures, thereby affecting regional runoff-ponding processes and groundwater infiltration patterns. Accurate assessment of infiltration volumes in subsidence zones under heavy rainfall is crucial for [...] Read more.
Surface subsidence induced by underground mining in mining areas significantly alters surface topography and hydrogeological conditions, forming depressions and fissures, thereby affecting regional runoff-ponding processes and groundwater infiltration patterns. Accurate assessment of infiltration volumes in subsidence zones under heavy rainfall is crucial for designing underground drainage systems and evaluating water-inrush risks in open-pit to underground transition mines. Taking the surface subsidence area of the Dahongshan Iron Mine as a case study, this paper proposes a rainfall infiltration calculation method based on the precise delineation of surface ponding-infiltration zones. By numerically simulating the subsidence range, the study divides the area into two distinct infiltration characteristic zones under different mining states: the caved zone and the water-conducting fracture zone. The rainfall infiltration volume under storm conditions was calculated separately for each zone. The results indicate that high-intensity mining-induced subsidence leads to a nonlinear surge in stormwater infiltration, primarily due to the significant expansion of the highly permeable caved zone. The core mechanism lies in the area expansion of the caved zone as a rapid infiltration channel, which dominates the overall infiltration capacity multiplication. These findings provide a scientific basis for the design of mine drainage systems and the prevention of water-inrush disasters. Full article
(This article belongs to the Special Issue Rock Mechanics and Mining Engineering)
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19 pages, 935 KB  
Article
Effect of Nutrient Supplementation on the Biochemical Composition and Microbial Safety of Open-Pond Spirulina Cultivated in Cameroon
by Tchouli Noufeu, Tinghong Ming, Xiaoqun Zeng, Jiajie Xu, Mbezele Junior Yannick Ngaba, François Tchoumbougnang, Achille Njomoue Pandong, Salle Mpondo, Tchoulabi Keyeya and Moussa Gouife
Foods 2025, 14(17), 3009; https://doi.org/10.3390/foods14173009 - 28 Aug 2025
Viewed by 719
Abstract
Spirulina (Arthrospira platensis) is globally recognized for its high nutritional value and potential as a sustainable food source. However, the influence of targeted nutrient supplementation on its biochemical composition and microbial safety under tropical open-pond conditions remains underexplored, particularly in sub-Saharan [...] Read more.
Spirulina (Arthrospira platensis) is globally recognized for its high nutritional value and potential as a sustainable food source. However, the influence of targeted nutrient supplementation on its biochemical composition and microbial safety under tropical open-pond conditions remains underexplored, particularly in sub-Saharan Africa. This study evaluated the effects of three nutrient supplementation regimes (compositions A, B, and C) and a control on Spirulina cultivated over 30 days in raceway ponds at the Nomayos Spirulina Production Farm in Cameroon. All treatments maintained physicochemical parameters within ranges favorable for Spirulina growth. Composition A significantly enhanced protein content (60.38 ± 0.68%), while composition C promoted carbohydrate accumulation (28.02 ± 0.41%). Microbial assessments revealed variable contamination levels, with composition B exhibiting the highest Escherichia coli (1.05 ± 0.075 × 105 CFU/g) and Salmonella/Shigella (4.09 ± 1.81 × 105 CFU/g) counts, potentially due to nutrient-induced changes or post-harvest handling factors. Correlation analyses revealed a moderate positive relationship between nitrogen input and protein synthesis (r = 0.309), which was not statistically significant (p = 0.329). Additionally, higher pH was significantly correlated with total mesophilic counts (r = 0.661, p = 0.019) and E. coli (r = 0.655, p = 0.020). These findings highlight the importance of nutrient formulation and environmental management in improving nutritional quality while minimizing microbial risks during Spirulina cultivation in tropical, low-tech settings. Full article
(This article belongs to the Section Food Nutrition)
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20 pages, 3721 KB  
Article
Removal of Co-Occurring Microplastics and Metals in an Aqueous System by Pristine and Magnetised Larch Biochar
by Stuart Cairns, Peter J. Holliman, Iain Robertson and Benjamin Harrison
Microplastics 2025, 4(3), 54; https://doi.org/10.3390/microplastics4030054 - 26 Aug 2025
Viewed by 683
Abstract
Microplastics and metals are increasingly recognised as major water contaminants with profound environmental and health consequences. The environmental co-occurrence of microplastics and metals are well documented in waterways, including urban runoff, highway balancing ponds, industrial wastewater, and mine-impacted waters, posing a multifaceted environmental [...] Read more.
Microplastics and metals are increasingly recognised as major water contaminants with profound environmental and health consequences. The environmental co-occurrence of microplastics and metals are well documented in waterways, including urban runoff, highway balancing ponds, industrial wastewater, and mine-impacted waters, posing a multifaceted environmental threat. Urgent remedial action is required to remove co-occurring microplastics and metals from water, giving consideration to how their co-occurrence can affect remediative efforts. However, information on the sorption of microplastics and Pb and Zn simultaneously by biochar is lacking. In this current study, changes in the quantity of metal adsorbed by pristine larch biochar and magnetised larch biochar due to the presence of microplastics was assessed using spectroscopic techniques. This study demonstrated that magnetised larch biochar and pristine larch biochar both remove co-occurring microplastics, Pb, and Zn from solution. Neither magnetised larch biochar nor pristine larch biochar show any statistical difference in the sorption of Pb with the inclusion of microplastics into the aqueous matrix. However, the inclusion of microplastics result in the reduced sorption of Zn by 43% for magnetised larch biochar (p < 0.01) and 69% for pristine larch biochar (p < 0.01). Magnetised larch biochar also demonstrated greater sorption than pristine larch biochar for microplastics (p < 0.05), Zn co-occurring with microplastics (p < 0.05), and Zn with no microplastics present (p < 0.01). Despite the effects of competitive sorption between Zn and microplastics, the removal of Pb, Zn, and microplastic from a multi-contaminant system indicate that magnetic larch biochar is a viable option to remove multiple contaminants from aqueous environs where metals and microplastics are seen to co-occur. Full article
(This article belongs to the Collection Feature Papers in Microplastics)
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34 pages, 1661 KB  
Review
Algae to Biofuels: Catalytic Strategies and Sustainable Technologies for Green Energy Conversion
by Shushil Kumar Rai, Gyungmin Kim and Hua Song
Catalysts 2025, 15(9), 806; https://doi.org/10.3390/catal15090806 - 25 Aug 2025
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Abstract
The global population surge and continuously rising energy demand have led to the rapid depletion of fossil fuel reserves. Over-exploitation of non-renewable fuels is responsible for the emission of greenhouse gases, air pollution, and global warming, which causes serious health issues and ecological [...] Read more.
The global population surge and continuously rising energy demand have led to the rapid depletion of fossil fuel reserves. Over-exploitation of non-renewable fuels is responsible for the emission of greenhouse gases, air pollution, and global warming, which causes serious health issues and ecological imbalance. The present study focuses on the potential of algae-based biofuel as an alternative energy source for fossil fuels. Algal biofuels are more environmentally friendly and economically reasonable to produce on a pilot scale compared to lignocellulosic-derived biofuels. Algae can be cultivated in closed, open, and hybrid photobioreactors. Notably, high-rate raceway ponds with the ability to recycle nutrients can reduce freshwater consumption by 60% compared to closed systems. The algal strain along with various factors such as light, temperature, nutrients, carbon dioxide, and pH is responsible for the growth of biomass and biofuel production. Algal biomass conversion through hydrothermal liquefaction (HTL) can achieve higher energy return on investments (EROI) than conventional techniques, making it a promising Technology Readiness Level (TRL) 5–6 pathway toward circular biorefineries. Therefore, algal-based biofuel production offers numerous benefits in terms of socio-economic growth. This review highlights the basic cultivation, dewatering, and processing of algae to produce biofuels using various methods. A simplified multicriteria evaluation strategy was used to compare various catalytic processes based on multiple performance indicators. We also conferred various advantages of an integrated biorefinery system and current technological advancements for algal biofuel production. In addition to this, policies and market regulations are discussed briefly. At the end, critical challenges and future perspectives of algal biorefineries are reviewed. Algal biofuels are environmentally friendly as well as economically sustainable and usually offer more benefits compared to fossil fuels. Full article
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