Search Results (1,044)

Population irruptions of crown-of-thorns starfish (CoTS; Acanthaster spp.) represent a perennial threat to Indo-Pacific coral reefs, often causing extensive coral loss and contributing to reef degradation. Therefore, extensive efforts are being made to contain population irruptions of CoTS either by culling or removing individual starfish across large reef areas. However, the efficacy and effectiveness of these management strategies are inherently constrained by limited detectability, even among adult starfish. This study adds to the limited quantitative data on the detectability of CoTS based on two independent intensive experimental studies conducted on Australia’s Great Barrier Reef. During depletive sampling (where all CoTS detected were removed prior to re-surveying the same area) over 3 days at Lizard Island, a total of 96 (out of 132) CoTS were recorded during initial surveys, and the average detectability across 34 transects where CoTS were recorded was estimated to be 78.4% (±13.4 SE). Estimated detectability declined to 64.4% (±11.22 SE) on day 2, suggesting that the remaining CoTS were more cryptic. During mark–recapture studies at Rib Reef, the total sample population was estimated to comprise 411 individuals, of which 266 (64.7%) were sighted during initial (day-time) surveys, while 322 (78.3%) were sighted at night. Average detectability across all surveys was estimated to be 75.9% (±0.05 SE). Our findings reaffirm that the detectability of adult CoTS is limited, which will inherently constrain the effectiveness of culling and removal. Full article

Background: A thorough post-myocardial infarction (MI) evaluation is essential for prognosis and rehabilitation. While cardiopulmonary exercise testing (CPET) is the standard for assessing functional capacity, combining it with dynamic stress echocardiography (DSE) may offer a more comprehensive assessment. Aim: This study examined the role of stress echocardiography (SE) in male post-MI patients by evaluating left ventricular function with conventional indices and the change in global longitudinal strain (ΔGLS) at rest and during maximal treadmill CPET. A secondary aim was to determine whether ΔGLS could provide additional value to traditional measures in post-MI care. Methods: Eighteen men with a recent MI [15 ST-elevation MI, three non-ST-elevation MI; mean age 53.2 ± 5.9 years, mean body mass index (BMI) 27.9 ± 2.2, 44.4% with a smoking history) and 18 age-matched male controls (mean age 50.1 ± 10.8 years, mean BMI 26.5 ± 2.4, 39.0% with smoking history) were enrolled. All MI patients were under optimal medical therapy, including β-blockers, which were withheld on the test day. Most underwent percutaneous coronary intervention (PCI), coronary artery bypass grafting (CABG) n = 2, or PCI for non-ST-elevation MI (NSTEMI) n = 3. Left ventricular ejection fraction (LVEF) and global longitudinal strain (GLS) were measured at rest and at peak effort and correlated with CPET parameters. Results: Post-MI patients had lower LVEF (50.6% vs. 60.7% at rest; 55.3% vs. 67.4% at peak, both p < 0.001), impaired GLS (–14.7% vs. –20.2% at rest, p = 0.003; –15.8% vs. –22.7% at peak, p = 0.001), and reduced VO₂peak (29.2 vs. 41.9 mL/kg/min, p < 0.001) compared with controls. In the MI group, ΔGLS correlated with VO₂peak (r = –0.645, p = 0.003) and VE/VCO₂ (r = 0.539, p = 0.020), indicating its potential as a marker of functional reserve. Conclusions: Combined CPET and SE offered comprehensive insights into functional and myocardial performance, identifying ΔGLS as a useful non-invasive index for risk stratification and rehabilitation after MI, with high feasibility and safety. Full article

Background and Objectives: To analyze changes in risk factors for refractive errors among Korean adolescents before and after the COVID-19 pandemic and examine the impact of lifestyle modifications on myopia development, Methods: this cross-sectional study utilized nationally representative data from the Korea National Health and Nutrition Examination Survey (KNHANES) VII (2016) and VIII (2021). We analyzed 691 adolescents aged 10–18 years from 2016 (pre-COVID-19) and 490 from 2021 (post-COVID-19). Refractive errors were categorized as hyperopia (≥+0.50 D), emmetropia (−0.50 to +0.50 D), myopia (−6.00 to −0.50 D), and high myopia (≤−6.00 D). Complex sample linear regression analyses identified factors associated with spherical equivalent (SE) refractive errors. Results: At the population level, overall myopia prevalence declined from 84.2% in 2016 to 77.4% in 2021, whereas the prevalence of high myopia increased from 10.0% to 11.5% (p = 0.047). This indicates that although the absolute proportion of adolescents with myopia decreased, the relative contribution of high myopia to the overall myopia burden within this population increased. Mean SE was −2.77 ± 0.11 D in (−10.63~+3.00 D/median: −2.00 D) 2016 and −2.63 ± 0.13 D (−14.00~+1.63/median: −1.75 D) in 2021 (p = 0.443). Age-related myopia progression accelerated post-pandemic (−0.193 D to −0.324 D per year in univariate regression and −0.185 D to −0.312 D, in multivariate regression analysis, p < 0.001). In both the 3 h and ≥4 h near work groups, statistically significant associations were observed at both time points, but a greater myopic shift was evident after COVID-19 (B = –0.853 and –0.757 in 2016; B = –1.311 and –1.167 in 2021, p < 0.05). Conclusions: The COVID-19 pandemic altered myopia risk factors among Korean adolescents. High myopia prevalence increased despite overall myopia with underweight status and environmental factors such as digital device time and urban living identified as important considerations for post-pandemic myopia prevention. Full article

This meta-analysis reports on the effect of professional development (PD) on K-12 in-service STEM teachers’ self-efficacy. There were 18 selected empirical studies in this study. Overall, PD had a modest positive effect on self-efficacy (Hedges’g = 0.551, 95% CI [0.285, 0.704], SE = 0.107) under the random-effects model. Furthermore, the findings show that (1) participant size of PD significantly contributed to the effect size of PD; (2) training hours of PD significantly contributed to the effect size of PD; (3) PD using the Teaching Efficacy Belief Instrument or other scales of self-efficacy showed larger significant effect sizes than PD using the Teachers’ Sense of Efficacy Scale. This study offers insights into the design of effective PD to improve STEM teachers’ self-efficacy. Full article

In recent years, the amount of electricity produced by photovoltaic systems in Poland has increased significantly. This paper presents an evaluation of commercial software (PVGIS 5.3, ENERAD, and PVGIS 24) used for simulating energy produced by four photovoltaic installations. The results of the simulation were compared with the real energy production. The installations differ in terms of panel orientation (S, SE, SE-NW), tilt angle (12°, 25°, 37°) and location (roof- or ground-mounted). The average annual electricity production per 1 kW of module power for each installation was as follows: PV1—1104 kWh·kW⁻¹, PV2—1169 kWh·kW⁻¹, PV3—927 kWh·kW⁻¹, and PV4—831 kWh·kW⁻¹. The highest values were recorded for ground-mounted installations facing south. Simulations carried out using computer programs show differences between simulated and real electricity production values of 35–41% for the ENERAD software, 3–13% for the PVGIS 5.3 software, and 3–32% for the PVGIS 24 software. The most accurate forecasts were obtained for the PV2 system in the PVGIS 24 software (MPE 3%, RMSE 12%), and the most unfavorable for the same installation in the ENERAD software (MPE 41%, RMSE 48%). Full article

Understanding the dose of ambient PM_2.5 inhaled by middle school students during active commuting between home and school is essential for optimizing their travel routes and reducing associated health risks. However, accurately modeling this remains challenging due to the difficulty of measuring ambient PM_2.5 concentrations along commuting routes at a population scale. In this study, we overcome this limitation by employing spatiotemporally dense observations of on-road ambient PM_2.5 concentrations collected through a massive mobile monitoring fleet consisting of around 200 continuously operating taxis installed with air quality monitoring instruments. Leveraging these rich on-road PM_2.5 observations combined with a GIS-terrain-based PM_2.5 dosage modeling approach, we (1) assess middle school students’ PM_2.5 dosages during morning (7:00 am–8:00 am) home–school walking commuting along the shortest-distance route; (2) examine the feasibility of identifying an alternative route for each student that minimizes PM_2.5 dosages during commuting; (3) investigate the trade-off between the relative reduction in PM_2.5 dosage and the relative increase in route length when opting for the alternative lowest-dosage route; and (4) examine whether exposure inequalities exist among students of different family socioeconomic statuses (SES) during their home–school commutes. The results show that (1) 18.8–57.6% of the students can reduce the dose of PM_2.5 by walking along an alternative lowest-dose route; (2) an alternative lowest-dose route could be found by walking along a parallel, less-polluted local road or walking on the less-trafficked side of the street; (3) seeking an alternative lowest-dose route offers a favorable trade-off between effectiveness and cost; and (4) exposure inequities do exist in a portion of students’ walking commutes and those students from higher-SES are more likely to experience higher exposure risks. The findings in our study could offer valuable insights into commuter exposure and inspire future research. Full article

This study examined muscle coordination during cutting movements in athletes with a history of groin pain. A total of 15 athletes who had experienced groin pain in the past two years (GP) and 14 healthy controls (CON) participated. Electromyography (EMG) and ground reaction force (GRF) data were collected, and EMG was analyzed using non-negative matrix factorization to extract muscle synergies. Three synergies were identified in both groups: Synergy 1 (landing), Synergy 2 (deceleration), and Synergy 3 (acceleration). No group differences were observed in GRF. However, compared with the CON, the GP demonstrated a 58.1% greater contribution of the latissimus dorsi and a 31.5% greater contribution of the erector spinae (SES) in Synergy 1, suggesting excessive trunk involvement during landing. In Synergy 2, SES contribution was 97.0% lower in the GP. In Synergy 3, the external oblique contribution decreased by 118.4%, while rectus abdominis contribution increased by 54.3%. These muscles are critical for pelvic stability, and their altered contributions indicate disrupted neuromuscular coordination in athletes with GP. Full article

This study proposes a novel framework to enhance inner race fault features in servo motor bearings by acquiring rotary encoder-derived instantaneous angular speed (IAS) signals, which are obtained from a servo motor encoder without requiring additional external sensors. However, such signals are often obscured by strong periodic interferences from motor pole-pair and shaft rotation order components. To address this issue, three key improvements are introduced within the cyclic blind deconvolution (CYCBD) framework: (1) a comb-notch filtering strategy based on rotation domain synchronous averaging (RDA) to suppress dominant periodic interferences; (2) an adaptive fault order estimation method using the autocorrelation of the squared envelope spectrum (SES) for robust localization of the true fault modulation order; and (3) an improved envelope harmonic product (IEHP), based on the geometric mean of harmonics, which optimizes the deconvolution filter length. These combined enhancements enable the proposed improved CYCBD (ICYCBD) method to accurately extract weak fault-induced cyclic impulses under complex interference conditions. Experimental validation on a test rig demonstrates the effectiveness of the approach in enhancing and extracting the fault-related features associated with the inner race defect. Full article

Background and Objectives: Children with autism spectrum disorder (ASD) often experience visual problems, yet their ophthalmic health remains underexplored due to testability challenges and limited-service access. This study evaluated ophthalmic screening outcomes in children with ASD and examined whether autism severity influenced ocular findings or cooperation during examinations. Materials and Methods: This cross-sectional study included 210 children with ASD (mean age 8.18 ± 4.99 years; 83.3% male). Examinations were conducted in an autism education center using non-contact methods: stereopsis (LANG I stereotest; LANG-STEREOTEST AG, Küsnacht, Switzerland), cover–uncover, and Hirschberg tests for strabismus, Spot Vision Screener (Welch Allyn Inc., Skaneateles Falls, NY, USA) for refractive errors, and Brückner test for red reflex. Autism severity was assessed with the Turkish version of the Adapted Autism Behavior Checklist (AABC). Results: Refractive errors were identified in 22.3% of participants: astigmatism in 15.2%, myopia in 5.2% (including 3 high myopia), and hyperopia in 1.9%. Strabismus was present in 11.9%, most commonly intermittent exotropia. Nearly half (49.5%) could not complete stereopsis testing, and a weak positive correlation was observed between AABC scores and the higher absolute spherical equivalent (SE) value between the two eyes (r = 0.173, p = 0.044). Children unable to complete stereopsis testing had significantly higher AABC scores (22.66 ± 9.69 vs. 13.39 ± 9.41, p < 0.001). Notably, 50 children (23.8%) had never undergone an eye examination prior to this study. Conclusions: Ophthalmic findings, particularly astigmatism and strabismus, are common in children with ASD. Greater autism severity was associated with reduced testability and modestly worse refractive error status. These findings suggest that tailored, accessible eye-care approaches and systematic vision screening may help to reduce overlooked visual problems and support more equitable care for children with ASD. Full article

Dried orange pulp (Citrus sinensis) is known for its antioxidant properties. This study aimed to examine the effects of adding dried orange pulp (OP) to the layers’ diets on the concentration of selected elements in the egg. The present work was part of a bigger project aiming to investigate the effect of orange pulp in layers’ diets on the performance of birds and egg quality. There were three dietary treatments and 63 layers per treatment, with 189 layers in total. Cages were the experimental units, and seven cages were allocated per treatment (n = 7). The dietary treatments were (1) a control treatment (C) that involved a basal diet without orange pulp addition, (2) an OP treatment with the addition of 9% dried orange pulp, and (3) a hesperidin–naringin (EN) treatment with 0.767 g hesperidin and 0.002 g naringin added per kg of diet; these levels of hesperidin and naringin represent those present in dried orange pulp for the OP treatment. Birds were fed the diets for 30 days. The diets had similar energy and protein levels and contained the same vitamin and mineral premixes. The analyzed egg (yolk, albumen, shell) elemental profile consisted of As, Ca, Cd, Co, Cr, Cu, Fe, Mg, Mn, Ni, Sb, Se, Sr, V, and Zn and was determined via Inductively Coupled Plasma Mass Spectrometry (ICP-MS). Dried orange pulp supplementation significantly altered the elemental profile. OP largely altered the element concentrations in albumen and egg yolk. Most notably, it decreased the concentrations of Co (p < 0.001), Fe (p < 0.001), Mn (p < 0.001), Ni (p = 0.046), and Se (p = 0.035) in egg yolk and those of Co (p = 0.011), Fe (p = 0.025), Cr (p = 0.049), Cu (p = 0.001), and Se (p = 0.014) in albumen. In addition, it decreased the concentrations of As (p = 0.025) and Ca (p = 0.025) in the eggshell. Principal component analysis was applied to the concentrations of the examined elements in all egg parts to explore the relationships between the elements and detect those capable of distinguishing samples, resulting in the apparent separation of yolk, albumen, and eggshell samples. Further analysis revealed that all samples were clustered into the three dietary treatments, resulting in 100% correct classification. The chelating and antioxidant capacities of flavonoids are intricate and rely on a variety of factors. OP supplementation modulated the deposition of specific elements in egg parts in comparison to those from layers fed a typical diet. Thus, this study indicated that eggs with specialized elemental profiles could be created. Full article

To express and purify staphylococcal enterotoxin M (SEM) using immobilized metal affinity chromatography (IMAC), a signal peptide-truncated (ΔNsp) wild-type SEM (SEM_WT) was N-terminally fused in pET-28a(+) to a polyhistidine tag (His_6×-) and thrombin cleavage site (TCS; LVPR↓GS), generating His_6×-TCS-ΔNspSEM_WT. Unexpectedly, 4 °C desalting reduced the fusion protein’s molecular weight by ~2.0 kDa on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). N-terminal sequencing and mass spectrometry identified cleavage specifically at the arginine (R) and glycine (G) peptide bond (R–G bond) within the TCS motif. AlphaFold 3 revealed an exposed serine protease catalytic triad: histidine 172, serine 178, and aspartic acid 212 (H₁₇₂/S₁₇₈/D₂₁₂) in the β-grasp domain, suggesting intrinsic thrombin-like activity (TLA). Sequential IMAC and size-exclusion high-performance liquid chromatography (SE-HPLC) purification eliminated contaminant concerns, while chromogenic substrate S-2238 (S-2238) assays demonstrated increasing specific activity and purification fold, supporting intrinsic TLA. Critically, the mutation of serine at position 178 to alanine (His_6×-TCS-ΔNspSEM_S178A) abolished TLA but preserved the secondary/tertiary structure, confirming the activity’s origin within the wild-type construct. Molecular dynamics (MD) simulations probed the atomistic mechanism for specific R–G bond cleavage. This work establishes a foundation for understanding ΔNspSEM_WT’s TLA. Full article

The formation and growth processes of octaneselenolate (C8Se) self-assembled monolayers (SAMs) on Au(111) from dioctyl diselenides were investigated by scanning tunneling microscopy (STM) as a function of vapor deposition time at 363 K. STM observations revealed unique surface features of the C8Se SAMs on Au(111) prepared at 363 K for 1 h, consisting of various types of narrow, bright molecular rows and broad, bright molecular rows with three-directional orientations. After increasing the deposition time from 1 and 6 h, interestingly, the structural quality of C8Se SAMs is greatly enhanced, showing the formation of tri-directional highly ordered domains with distinct domain boundaries on an entire Au(111) surface. The observed ordered phase can be described by a (2 × 2√7)rect packing structure with an arial density of 28.9 Ų/molecule. C8Se SAMs at 363 K for 24 h consisted of long-range, highly ordered domains and large, disordered domains. The ordered phase can be described by a (√3 × √23)rect packing structure with an arial density of 23.3 Ų/molecule, which is denser than that of C8Se SAMs formed by vapor deposition at 363 K and for 6 h. This study clearly demonstrates that vapor deposition is a highly effective method for preparing highly ordered alkyl selenolate SAMs on Au(111). Furthermore, molecular-scale STM results provide new insights into the formation and growth processes of alkyl selenolate SAMs on Au(111) formed by vapor deposition of dialkyl diselenides at high temperatures. Full article

Quercus suber L. (Q. suber) is an ecologically and industrially valuable species, yet faces challenges in propagation in China. This study optimized somatic embryogenesis (SE) protocols using two-year-old Q. suber leaves, focusing on petioles and leaf veins as the most responsive explants, with May as the optimal sampling time. The MSSH medium (a combination of Murashige and Skoog Medium (MS) major elements and Schenk and Hildebrandt Medium (SH) minor elements and vitamins) under darkness maximized transdifferentiation. Additionally, the highest callus induction was achieved with 0.50 mg/L 6-benzyladenine (6-BA) and 1.00 mg/L 1-Naphthaleneacetic acid (NAA). Liquid culture with 1.00 g inoculum and 0.50 mg/L 6-BA + 0.20 mg/L NAA achieved the best proliferation. Redifferentiation peaked at 0.15 mg/L NAA + 0.20 mg/L 6-BA. Transcriptome profiling identified 4534 differentially expressed genes (DEGs) between embryogenic callus (E1) and global embryos (E2), with key pathways linked to cell wall remodeling, stress responses, and photosynthesis. Key regulators identified during the early stage of callus redifferentiation include cytokinin oxidase 3 (CKX3), gibberellin-responsive protein (GH3.6), and pectin lyase 5 (PL5), among others. This study provides insights into efficient SE of Q. suber and the genes underlying early callus redifferentiation, laying the groundwork for future research. Full article

To evaluate the effects of dietary threonine levels on rice field eels (Monopterus albus), six experimental diets were formulated with graded threonine supplementation: 0 g/kg (T1), 3 g/kg (T2), 6 g/kg (T3), 9 g/kg (T4), 12 g/kg (T5), and 15 g/kg (T6). Rice field eels, with an initial body weight of 18.47 ± 0.11 g, were fed for 60 days. The weight gain ratio (WGR), specific growth rate (SGR), and protein efficiency ratio (PER) significantly increased in the T4 group, while feed conversion ratio (FCR) significantly decreased. Broken-line regression analysis determined the optimal dietary threonine requirement to be 7.5–9.0 g/kg. Concomitantly, whole-body crude protein content increased, while lipid content decreased; serum GOT and GPT activities declined, indicating reduced hepatic stress, whereas hepatic CAT and SOD activities markedly increased, enhancing antioxidant capacity. Digestive enzyme capacity was enhanced, as evidenced by elevated lipase and trypsin activities. Muscle texture properties, including cohesiveness, springiness, and chewiness, were significantly improved in the T4 and T5 groups. At the molecular level, MyoG, MyoD1, and MYF5 expression peaked in T4, while MRF4 expression increased progressively. LEfSe analysis of the microbiome, coupled with KEGG functional prediction, revealed that Corynebacterium and Methylocella were positively correlated with growth metrics; these genera likely promote muscle deposition through three enriched metabolic pathways that enhance energy and amino acid availability. Collectively, dietary threonine supplementation at 7.5–9.0 g/kg promotes growth, enhances digestive function, and improves muscle quality in rice field eels. Full article

Background: Cadmium (Cd) exposure is linked to cognitive decline in middle-aged and older adults, but the modifying role of essential minerals is unclear. This study aimed to identify key protective minerals and quantify their joint antagonistic effect against Cd neurotoxicity. Methods: Baseline serum minerals and urinary Cd were measured in 6795 adults (≥40 years) from the 2015 China Health and Nutrition Survey. Cognitive function (MMSE) was assessed after 3 years. Associations were analyzed using multiple linear regression and Quantile g-computation (QGC) for joint effects. Combined exposure groups and interaction terms were assessed. Restricted cubic spline (RCS) models explored potential nonlinear dose–response relationships. Results: Participants in the highest urinary Cd quartile had significantly lower MMSE score (β = −0.09, 95% CI: −0.15, −0.02) than the lowest quartile. Serum calcium (Ca), ferrum (Fe), magnesium (Mg), selenium (Se), and phosphorus (P) were positively associated with MMSE. QGC revealed that the joint effect of Cd and the 5-mineral mixture (β = 0.10, 95% CI: 0.05, 0.14) was weaker than the protective effect of the 5-mineral mixture. Any high-mineral group had significantly higher MMSE score compared to the high-Cd/low-mineral group. Conclusions: Essential minerals Ca, Fe, Mg, Se, and P effectively antagonize Cd-associated cognitive decline. Their combined exposure demonstrates significant protective effects, providing key evidence for precision nutrition and environmental health risk management in Cd-exposed populations. Full article