Search Results (14,061)

Historically, the Mediterranean Sea has been an area of cultural exchange and maritime commerce. One out of many submerged archaeological sites is the Roman shipwreck that was discovered in 2006 off the coast of Santo Stefano al Mare, in the Ligurian Sea, Italy. The wreck was dated to the 1st century B.C. and consists of a well-preserved cargo ship of Roman amphorae that were likely used for transporting wine. In this study, we present the results of the first underwater survey of the wreck using an Autonomous Underwater Vehicle (AUV) industrialized by Graal Tech. The AUV was equipped with a NORBIT WBMS multibeam sonar, a 450 kHz side-scan sonar, and inertial navigation systems. The AUV conducted multiple high-resolution surveys on the wreck site and the collected data were processed using geospatial analysis methods to highlight local anomalies directly related to the presence of the Roman shipwreck. The main feature was an accumulation of amphorae, covering an area of approximately 10 × 7 m with a maximum height of 1 m above the seabed. The results of this interdisciplinary work demonstrated the effectiveness of integrating AUV technologies with spatial analysis techniques for underwater archaeological applications. Furthermore, the success of this mission highlighted the potential for broader applications of AUVs in the study of the seafloor, such as monitoring seabed movements related to offshore underground energy storage or the identification of objects lying on the seabed, such as cables or pipelines. Full article

Major depression (MD) is associated with chronic inflammation and impaired neuroplasticity; however, the cellular mechanisms underlying antidepressant action remain incompletely understood. We performed transcriptomic profiling and functional validation in human microglia treated with venlafaxine (VEN) and vortioxetine (VOR), or with stable ST3GAL5 overexpression (ST3GAL5OE). Differential expression analysis, enrichment studies, and functional assays using NF-κB-RE-NlucP and SIE-NlucP reporter lines were conducted to assess the impact on inflammatory signaling. Microarray analysis identified 41 genes consistently upregulated and 316 consistently downregulated across VEN, VOR, and ST3GAL5OE conditions. Upregulated genes were enriched for synaptic organization, whereas downregulated genes were associated with nitric oxide biosynthesis and pro-inflammatory pathways, including Rap1, MAPK, and PI3K-Akt signaling. Functional assays confirmed that VEN and VOR suppressed cytokine-induced NF-κB and STAT3 activation, effects that were recapitulated by exogenous GM3 treatment and ST3GAL5 overexpression. Chronic exposure to VEN or VOR produced more modest, pathway-specific suppression, supporting convergence on the ST3GAL5–GM3 axis. These findings extend the conventional monoaminergic model of antidepressant action by highlighting the ST3GAL5–GM3 lipid remodeling axis as a novel regulatory pathway that attenuates microglial inflammatory signaling. Although validation in primary microglia and in vivo models is required, our results suggest that this axis could serve as both a therapeutic target and a candidate biomarker for inflammation-associated MD. Full article

Here, 18S-rDNA sequences of Blastocystis sp., previously documented from symptomatic (cases) and asymptomatic (controls) carriers, were analyzed to determine their population structure, predict their secondary structure, and examine their interactions with ribosomal proteins (Bud23, RPS5, and RPS18). Phylogenetic and population differentiation analyses were performed using STRUCTURE software V2.3.4. Moreover, an analysis of the rRNA secondary structure and folding of each sequence was performed, and their probability of interaction with ribosomal proteins was determined. Phylogenetic and haplotype analyses sorted the sequences into genetic subtypes ST1, ST2, and ST3, while the population structure showed each cluster as a differentiated subpopulation, suggesting incipient speciation or cryptic species differentiation. Furthermore, the analysis of the secondary structure of rRNA exhibited specific arrangements for each subtype. In addition, the probability of interaction between 18S-rRNA sequences of Blastocystis from cases and controls with RPS5 and RPS18 was significant, matching the biological plausibility of the previously documented finding that control isolates had a lower generation time than isolates obtained from cases. These findings reinforce the hypothesis that ribosomal subtypes ST1–ST3 of Blastocystis represent evolutionarily distinct lineages with the potential to be recognized as future species. Furthermore, they underscore the functional relevance of 18S-rRNA sequences from clinical isolates of Blastocystis. Full article

Programmable Logic Controllers (PLCs), widely used in industrial automation, are often programmed in IEC 61131-3 Structured Text (ST), which is prone to subtle logic vulnerabilities. Traditional tools like static analysis and fuzzing struggle with the complexity and domain-specific semantics of ST. This work explores Large Language Models (LLMs) for PLC vulnerability detection, supported by both theoretical insights and empirical validation. Theoretically, we prove that control flow features carry the most vulnerability-relevant information, establish a feature informativeness hierarchy, and derive sample complexity bounds. We also propose an optimal synthetic data mixing strategy to improve learning with limited supervision. Empirically, we build a dataset combining real-world and synthetic ST code with five vulnerability types. We fine-tune open-source LLMs (CodeLlama, Qwen2.5-Coder, Starcoder2) using LoRA, demonstrating significant gains in binary and multi-class classification. The results confirm our theoretical predictions and highlight the promise of LLMs for PLC security. Our work provides a principled and practical foundation for LLM-based analysis of cyber-physical systems, emphasizing the role of domain knowledge, efficient adaptation, and formal guarantees. Full article

Long-term greenhouse operations face a critical challenge in the form of soil quality degradation, yet the key intervention periods and underlying mechanisms of this process remain unclear. This study aims to quantify the effects of greenhouse lifespan on the evolution of soil quality and to identify critical periods for intervention. We conducted a systematic survey of greenhouse operations in a representative area of Yunnan Province, Southwest China, and adopted a space-for-time substitution design. Using open-field cultivation (OF) as the control, we sampled and analyzed soils from vegetable greenhouses with greenhouse lifespans of 2 years (G2), 5 years (G5), and 10 years (G10). The results showed that early-stage greenhouse operation (G2) significantly increased soil temperature (ST) by 8.38–19.93% and soil porosity (SP) by 16.21–56.26%, promoted nutrient accumulation and enhanced aggregate stability compared to OF. However, as the greenhouse lifespan increased, the soil aggregates gradually disintegrated, particle-size distribution shifted toward finer clay fractions, and pH changed from neutral to slightly alkaline, exacerbating nutrient imbalances. Compared with G2, G10 exhibited reductions in mean weight diameter (MWD) and soil organic matter (SOM) of 2.41–5.93% and 24.78–30.93%, respectively. Among greenhouses with different lifespans, G2 had the highest soil quality index (SQI), which declined significantly with extended operation; at depths of 0–20 cm and 20–40 cm, the SQI of G10 was 32.59% and 38.97% lower than that of G2, respectively (p < 0.05). Structural equation modeling (SEM) and random forest analysis indicated that the improvement in SQI during the early stage of greenhouse use was primarily attributed to the optimization of soil hydrothermal characteristics and pore structure. Notably, the 2–5 years was the critical stage of rapid decline in SQI, during which intensive water and fertilizer inputs reduced the explanatory power of soil nutrients for SQI. Under long-term continuous cropping, the reduction in MWD and SOM was the main reason for the decline in SQI. This study contributes to targeted soil management during the critical period for sustainable production of protected vegetables in southern China. Full article

Background/Objectives: Negative-Pressure Wound Therapy (NPWT) is increasingly used to promote wound healing in chronic and complicated wounds, but its use in surgical oncology is still debated due to theoretical concerns about promoting local tumor recurrence. The aim of this study is to review the available evidence on the oncologic safety of NPWT, specifically regarding the risk of local recurrence in patients undergoing surgery for cutaneous melanoma (CM) or soft tissue sarcoma (STS). Methods: A systematic review of the literature was conducted using the MEDLINE/PubMed, EMBASE, and Scopus databases through December 2024 (PROSPERO: CRD42024623405). Case series, retrospective cohort studies, and randomized clinical trials reporting survival data in melanoma and sarcoma patients treated with NPWT were eligible for inclusion. PRISMA guidelines were followed and quality assessment checked. Results: Seventeen studies were eligible for the analysis with a total of 285 patients, 197 affected by soft tissue sarcoma and 88 by cutaneous melanoma. The pooled proportion of local recurrence was 5% in patients treated with NPWT, regardless of the histology considered (STS and CM). When comparing NPWT to conventional wound therapy, both the pooled risk ratio (0.87; 95% CI: 0.24–3.11; Tau² = 0.14; I² = 8%) and odds ratio (0.83; 95% CI: 0.20–3.39; Tau² = 0.18) indicated no statistically significant difference in the recurrence rate. Conclusions: Current evidence does not suggest an increased risk of local recurrence associated with NPWT in melanoma or sarcoma patients, and mostly, NPWT may have important advantages over standard surgical dressings. More high-power randomized controlled trials with wider follow-up periods are needed to make it possible for practitioners to use this technique without being afraid of higher risk local recurrences. Full article

Hexaflumuron, a benzoylurea insecticide, exhibits high toxicity against various insect pests. Sublethal doses of hexaflumuron significantly impair nymphal development and subsequent fecundity in the resulting Apolygus lucorum (Meyer-Dür) adults. However, the effects on adults of A. lucorum that are exposed to sublethal concentrations of hexaflumuron are not known. In this study, we evaluated the toxicity of hexaflumuron to 1st- and 3rd-instar nymphs, as well as one-day-old adults, of A. lucorum using an artificial diet with hexaflumuron incorporated. Acute toxicity bioassays determined the median lethal concentration (LC₅₀) for 1st- and 3rd-instar nymphs of A. lucorum to be 0.311 and 0.337 mg/L. In contrast, the LC₅₀ level for one-day-old adults of A. lucorum was 13.193 mg/L. To evaluate the sublethal effects of hexaflumuron on 3rd-instar nymphs, we used LC₁₀, LC₂₀, and LC₅₀ concentrations (0.018, 0.127, and 0.337 mg/L), fed for 48 h. The exposure of 3rd-instar nymphs to the LC₅₀ concentration significantly reduced the fecundity of adults. We also exposed one-day-old adults to LC₁₀, LC₂₀, and LC₅₀ dietary concentrations (0.089, 4.587, and 13.193 mg/L) for 48 h. Exposure to the LC₁₀ and LC₂₀ concentrations caused significant reductions in longevity and fecundity in adult females. The LC₅₀ concentration also caused a significant reduction in the longevity of adult males and the egg hatching rate. An understanding of these sublethal impacts can facilitate the development of effective control strategies targeting A. lucorum. Full article

Background/Objectives: To compare the external load (EL) of elite youth soccer players during official international matches between age categories and playing positions. Methods: The sample consisted of 42 elite youth soccer players categorized by age categories, U-15, U-17 and U-20 and playing positions: central defender (CD); fullback (FB); midfielder (MF); wide attacker (WA) and striker (ST). The Vector X7 (Catapult Sports) device was used for collecting the following EL variables: total distance traveled (TD), player load (PL) and distance traveled per velocity band 0 to 7 km/h (D7); 7 to 13 km/h (D13); 13 to 19 km/h (D19); 19 to 23 km/h (D23) and >23 km/h (HSR). Linear mixed-effect models were applied to analyze the differences. Results: Large differences were found between positions (p < 0.01) in TD (η²p = 0.48), PL (η²p = 0.30), D19 (η²p = 0.44), D23 (η²p = 0.68) and HSR (η²p = 0.53). Large differences were found according to category between U-15 and U-17 in TD (p = 0.006 and η²p = 0.25) and D13 (p = 0.003 and η²p = 0.27). Large interaction effects were found in DT (p = 0.014 and η²p = 0.44) and D23 (p = 0.002 and η²p = 0.51). Conclusions: This study concludes that there are differences in EL in official matches in elite youth players between age categories and playing position. These differences can be applied in practice to design individualized training by playing position and to monitor EL during microcycles. Full article

Background: Up to 40% of people with hypertension (HTN) develop mild cognitive impairment and Alzheimer’s disease during their lifetime. This study aimed to compare physical and cognitive performance in older adults, classified as non-HTN or with HTN, under single-task (ST) and dual-task (DT) conditions. Methods: In total, 46 individuals (71 ± 5.96 years), divided equally into non-HTN and HTN groups, participated. Normality of the data was tested using the Shapiro–Wilk test. In this cross-sectional study, groups were compared using the Mann–Whitney U test applied to non-parametric variables and the independent samples t-test applied to parametric ones. Physical and cognitive functions were evaluated using the Short Physical Performance Battery (SPPB), HandGrip Strength (HGS), Timed Up and Go (TUG), and the L-Test, both in ST and DT conditions (with arithmetic tasks). Results: Significant differences were observed between groups in MoCA and the physical performance of SPPB, TUG, and L-Test under ST. In the DT condition, differences were found in the physical performance of TUG, L-test, and SPPB total score as well as in different components such as the 3 m walk and the Sit to Stand (STS). Regarding physical–cognitive interference, there was a statistically significant difference in the SPPB dual task cost between the HTN and non-HTN groups. Conclusions: Individuals with HTN exhibit impairments compared to non-HTN individuals in physical performance under DT conditions as well as in physical–cognitive interference. Static balance and HGS appear unaffected; however, differences are evident in gait (TUG and L-Test) and lower-limb strength (STS). Full article

Background/Objectives: Carbapenem-resistant Klebsiella pneumoniae (CRKP) threatens Intensive Care Units (ICU), particularly in settings where serine (KPC) and metallo-β-lactamases (NDM) co-circulate. The aim of this study was to assess CRKP susceptibility especially to novel β-lactam/β-lactamase inhibitor combinations, characterize the genetic determinants of resistance, and contribute to the understanding of local epidemiology in the ICU of our hospital. Methods: We studied 32 non-duplicate CRKP isolates (30 ICU, 2 wards) collected at Hippokration General Hospital, Thessaloniki (May–Oct 2023). Bacterial identification and Antimicrobial susceptibility testing (AST) were performed by VITEK-2; Minimum inhibitory concentrations (MICs) for ceftazidime/avibactam (CAZ/AVI), meropenem/vaborbactam (MER/VAB), and imipenem/relebactam (IMI/REL) were determined by E-tests. Colistin MICs were performed by broth microdilution. Carbapenemases were screened phenotypically and by immunochromatography and confirmed by multiplex PCR. One bronchial isolate co-harboring bla_NDM and bla_KPC genes underwent WGS. Results: All isolates were carbapenem-resistant and showed extensive resistance to β-lactams and fluoroquinolones. By PCR, 8/32 (25%) carried bla_KPC alone, 8/32 (25.0%) bla_NDM alone, and 16/32 (50%) co-harbored bla_KPC and bla_NDM. KPC-only isolates were generally susceptible in vitro to CAZ/AVI, MER/VAB, and IMI/REL, whereas dual KPC-NDM producers were resistant to all three combinations. Tigecycline showed the highest retained activity; colistin remained active in a minority. WGS of one ST512 (CG258) isolate revealed co-harboring bla_NDM-1 and bla_KPC-3 with additional resistance determinants and plasmid replicons, consistent with high-risk spread. Conclusions: Half of CRKP isolates in this ICU-predominant series co-produced KPC and NDM, severely limiting β-lactam/β-lactamase inhibitor options. These data support routine screening for carbapenemases, strict infection prevention, antimicrobial stewardship, and access to agents active against MBLs. Full article

Staurosporine (STS) was discovered in 1977 by Omura and colleagues during a chemical screening for microbial alkaloids. It was the first indolocarbazole compound isolated from a soil-dwelling bacterium, Streptomyces staurosporeus. STS was also found to have antifungal activity, but its potent protein kinase (PK) inhibitory properties, perhaps the most extensively characterized biochemical feature of STS, were only revealed nearly a decade after its discovery. Thereafter, STS has been studied mainly for its anticancer potential with foreseen applications ranging from biomedical (e.g., antiparasitic) to agricultural (e.g., insecticidal). Interestingly, the recent discovery that STS induces apoptosis in the filamentous fungus Neurospora crassa renewed interest in this molecule as a scaffold for antifungal drug development. Studies in fungi and mammalian cell lines suggest that, in addition to PK inhibition, other modes of action are possible for STS. These may involve the targeting of membrane lipid domains and/or alterations of membrane biophysical properties. Here, the studies on the action of STS and its natural and synthetic derivatives against diverse fungal species, since its discovery to the present day, are critically reviewed and discussed with the aim of highlighting their advantages, limitations to be overcome, conceivable mechanisms of action, and potential as antifungal chemotherapeutic agents. Full article

The growing need for safe, cheap and sustainable earthquake-resistant buildings means that efficient methods for optimal seismic design must be found. The complexity and nonlinearity of the problem can be addressed using advanced automated techniques. This paper presents an intelligent three-step procedure for optimally designing earthquake-resistant buildings based on the training (1st step) and successive inversion (2nd step) of Multi-Layer Perceptron Neural Networks. This involves solving the inverse problem of determining the optimal design parameters that meet pre-assigned, code-based performance targets, by means of a gradient-based optimization algorithm (3rd step). The effectiveness of the procedure was tested using an archetypal multistory, moment-resisting, concentrically braced steel frame with active tension diagonal bracing. The input dataset was obtained by varying four design parameters. The output dataset resulted from performance variables obtained through non-linear dynamic analyses carried out under three earthquakes consistent with the Chilean code spectrum, for all cases considered. Three spectrum-consistent records are sufficient for code-based seismic design, while each seismic excitation provides a wealth of information about the behavior of the structure, highlighting potential issues. For optimization purposes, only information relevant to critical sections was used as a performance indicator. Thus, the dataset for training consisted of pairs of design parameter sets and their corresponding performance indicator sets. A dedicated MLP was trained for each of the outputs over the entire dataset, which greatly reduced the total complexity of the problem without compromising the effectiveness of the solution. Due to the comparatively low number of cases considered, the leave-one-out method was adopted, which made the validation process more rigorous than usual since each case acted once as a validation set. The trained network was then inverted to find the input design search domain, where a cost-effective gradient-based algorithm determined the optimal design parameters. The feasibility of the solution was tested through numerical analyses, which proved the effectiveness of the proposed artificial intelligence-aided optimal seismic design procedure. Although the proposed methodology was tested on an archetypal building, the significance of the results highlights the effectiveness of the three-step procedure in solving complex optimization problems. This paves the way for its use in the design optimization of different kinds of earthquake-resistant buildings. Full article

Background/Objectives: Alcohol and smoking during pregnancy may be associated with several complications, but the underlying mechanism is still unclear. The aim of this study was to evaluate the role of oxidative stress induced by smoking and alcohol during pregnancy and their effects on fetal and neonatal outcomes. Material and methods: We considered pregnant women at term. Validated questionnaires were used to investigate smoking and alcohol habits. Ultrasound was performed to evaluate fetal weight, amniotic fluid index, and maternal-fetal Doppler velocimetry. At the time of delivery, we collected a tuft of maternal hair, maternal venous blood, and cord blood. In these samplings we determined in phase I nicotine, cotinine, and ethyl glucuronide on the maternal keratin matrix with the gas chromatography-mass spectrometry technique. In phase II, the Free Oxygen Radicals Test (FORT) and Free Oxygen Radical Defense (FORD) test were used to assess circulating reactive oxygen species (ROS). Results: 119 pregnant patients were enrolled (n = 62 for smoking and n = 57 for alcohol). Twenty-six patients (42%) out of 62 were active smokers. Three patients (5%) out of 57 were alcoholic consumers. Mean neonatal weight and mean placental weight were significantly lower for active smokers (p = 0.0001). The neonatal weight was in the 1st–2nd percentile for all alcohol abusers. Considering two subgroups (n = 10 non-smokers and n = 10 smokers) for ROS determination, a statistically significant higher oxidative stress in the blood of smoking patients was evidenced (p < 0.0001). In cord blood the differences were not statistically significant (p = 0.2216). Conclusions: Fetal growth restriction was present in the group of active smokers and in patients with alcohol abuse. Oxidative stress was higher in smoking patients than in non-smokers. However, in cord blood, FORT was negative in all cases, suggesting a protective mechanism in utero. Given the limited sample size, the results obtained are preliminary and require future studies. Full article

The result of accelerated development and technological progress is manifested through numerous changes in the labor market, primarily concerning the competencies of future employees. Many of those competencies have symmetrical character. The determinants that may influence the development of specific competencies are variable and dynamic, yet they share the characteristic of transcending temporal and spatial boundaries. In this paper we propose the use of a combination of Principal Component Analysis (PCA) and Fuzzy Analytic Hierarchy Process (FAHP) to rank 21st-century competencies that are developed independently of the formal educational process. Ability to organize and plan, appreciation of diversity and multiculturalism, and ability to solve problems appeared to be the highest-ranked competencies. The development of key competencies is symmetrical to the skills for the labor market. Also, the development of key competencies is symmetrical to the right selection of the quality of media content. The paper proves that the development of key competencies is symmetrical to the level of education of both parents. One of the key findings is that participants with higher levels of media literacy express more readiness for the contemporary labor market. Moreover, the family, particularly parents, exerts a highly significant positive influence on the development of 21st-century competencies. Parents with higher levels of education, in particular, provide a stimulating environment for learning, foster critical thinking, and encourage the exploration of diverse domains of knowledge. Full article

This study distinguished male and female individuals by wing morphology (males with long wings, females with short wings) and investigated sexual dimorphism in the chemosensory system of Blaptica dubia through integrated ultrastructural and transcriptomic analyses. Scanning electron microscopy (SEM) was used to characterize the type, number, and distribution of antennal sensilla, while Illumina HiSeq sequencing, Gene Ontology/Kyoto Encyclopedia of Genes and Genomes (GO/KEGG) annotation, and Quantitative Real-time Reverse Transcription Polymerase Chain Reaction (qRT-PCR) validation were employed to analyze sex-specific gene expression profiles. Both sexes exhibited Böhm’s bristles, chaetic, trichoid, and basiconic sensilla. Males showed significantly more chaetic sensilla on the pedicel and longer type I/II chaetic sensilla on the flagellum, whereas females had longer ST2 sensilla. Basiconic sensilla were predominantly flagellar-distributed and more abundant/longer in males. No sexual differences were observed in Böhm’s bristles. Transcriptomics revealed 5664 differentially expressed genes (DEGs) (2541 upregulated; 3123 downregulated), enriched in oxidation-reduction, extracellular space, lysosome, and glutathione metabolism. KEGG analysis identified five key pathways: lysosome, glutathione metabolism, cytochrome P450-mediated xenobiotic/drug metabolism, and ascorbate/aldarate metabolism. Among 11 chemosensory-related DEGs, chemosensory proteins (CSPs) and odorant binding proteins (OBPs) were downregulated in males, while gustatory receptors (GRs), olfactory receptors (Ors), and ionotropic receptors (IRs) were upregulated. These results demonstrate profound sexual dimorphism in both antennal sensilla morphology and chemosensory gene expression, suggesting divergent sex-specific chemical communication strategies in Blaptica dubia, with implications for understanding adaptive evolution in Blattodea. Full article