Search Results (628)

Cryopreservation of equine semen remains challenging due to pronounced individual variability in cryotolerance. Because freezing induces oxidative stress and spermatozoa are particularly susceptible to such damage, this study aimed to comparatively evaluate the effects of natural extracts from nutraceutical compounds with high antioxidant activity, specifically matcha, spirulina, and horseradish, as well as quercetin, a well-known antioxidant molecule. These compounds were added to the freezing extender, and semen from 12 Salernitano stallions (48 ejaculates in total) was analyzed. Several parameters were assessed, including sperm kinetics, bioenergetics, oxidative and nitrosative stress markers, and the sperm DNA fragmentation index, both before and after cryopreservation. Neither the natural extracts nor quercetin significantly improved sperm freezability, likely due to the high degree of inter-individual variability. Stallion age also had a significant effect on nearly all the parameters evaluated, although no significant interactions were observed between age and treatment for any of the sperm quality traits. In conclusion, supplementation of the freezing extender with matcha, spirulina, horseradish extracts, or quercetin did not significantly enhance stallion semen cryopreservation outcomes. Conversely, stallion age and individual variability had a marked effect on sperm cryotolerance, highlighting the need for customized and holistic strategies to optimize cryotolerance in individual stallions. Full article

Sustainability of animal production requires reducing reliance on soybean meal by identifying viable alternative protein sources. Within the framework of the Italian Agritech National Research Center, seven Italian research groups collaborated to evaluate unconventional feed ingredients and their effects on animal performance and product quality. Alternative legume seeds (peas, chickpeas, faba bean, and lupins) can partially or completely replace soybean meal without impairing productivity, while enhancing product health value and shelf-life through bioactive compounds. Microalgae (Chlorella, Spirulina) improved carotenoid content, antioxidant activity, fatty acid profile, and cholesterol levels in poultry products, with limited effects in pigs. Insects supported optimal growth in fish at 25–30% inclusion, whereas maximum recommended levels are 15% in broilers and 24% in laying hens to sustain growth, egg production, and quality. Camelina by-products are suitable for poultry diets at up to 5–10%, beyond which performance declines. Whole-plant soybean silage, tef (Eragrostis tef), and triticale–lupin intercropping represent promising protein-rich resources for ruminants, provided diets maintain balanced protein-to-energy ratios, adequate fibre characteristics, and appropriate harvest timing under drought-prone conditions. Collectively, these findings highlight the potential of diverse protein sources to improve the sustainability of livestock systems while preserving productivity and enhancing the nutritional quality of animal-derived foods. Full article

Spirulina extract holds significant promise for food applications, but its characteristic odor limits consumer acceptance. This study evaluated ozone (5, 10, and 25 ppm) and activated carbon (AC; 10, 30, and 50% w/v) treatments for their effects on selected volatile organic compounds (VOCs) in spirulina aqueous extracts, as well as on protein content, bioactive compounds, and antioxidant activities. Neither treatment adversely affected protein content. Ozone treatments significantly increased total phycobiliprotein content (172.5–181.1 mg/g; p < 0.05), whereas AC treatments significantly reduced it (138.5–159.0 mg/g; p < 0.05). Both treatments decreased chlorophyll (13.9–30.6%) and carotenoid (44.6–72.3%) levels, while DPPH and ABTS antioxidant activities varied according to treatment and concentration. AC treatments were more effective than ozone in reducing total VOCs (74.1–79.9% vs. 30.3–55.5% reduction), but 25 ppm ozone achieved the most favorable sensory profile as assessed by trained panelists. Treatments with 25 ppm ozone and 10% AC provided the best compromise between deodorization and retention of bioactive compounds. These findings indicate that both ozone and AC treatments can substantially reduce the undesirable odor of spirulina extracts, thereby improving their sensory quality and application potential in odor-sensitive food and functional products. Full article

The incorporation of Arthrospira platensis into dairy products offers health benefits but is limited by its undesirable aroma and flavor. This study evaluated three deodorization strategies—adsorption by activated carbon, extraction with ethanol, and fermentation with Saccharomyces cerevisiae—to improve the sensory profile of A. platensis and enhance its acceptability in yogurt. Deodorized powders were characterized for proximal and volatile composition and used to formulate yogurts at five concentrations (0.5–2.5% w/v). Texture, aroma volatile profile, and sensory attributes were assessed after yogurt production, while shelf-life quality attributes were monitored over 29 days of refrigerated storage. Yogurts containing fermented A. platensis showed higher sensory scores (>8.7/10), the greatest purchase intent (>71.4%), and improved texture, remaining acceptable at an addition level of 2.5%. In contrast, yogurts with untreated or carbon-activated treated A. platensis were only acceptable at low addition levels (≤0.5%) due to off-flavors and textural issues. Ethanol effectively reduced aldehydes and ketones (such as Safranal and β-Ionone), while fermentation eliminated pyrazines and generated desirable alcohols and acids (such as 1-Pentanol and Butanoic acid). These findings highlight fermentation as a promising strategy to deodorize A. platensis and improve its integration into dairy matrices, enabling the development of functional yogurts with enhanced sensory quality and nutritionally relevant microalgae content. Full article

This systematic review included 31 clinical trial articles examining the effects of natural compounds on Alzheimer’s disease (AD) and mild cognitive impairment (MCI), involving 3582 participants aged 50–90. Treatment durations ranged from 8 weeks to 2 years, with an average of 12.5 months. Notably, 11 studies focused on herbal extracts highlighting their prominence in current research. These extracts showed potential cognitive and neuroprotective benefits, although results varied across compounds and study designs. Other natural compounds—including flavonoids, polyphenols, omega-3 fatty acids, Aloe vera, Spirulina, and citrus phytochemicals—may provide cognitive and neuroprotective benefits, with ginseng and Ginkgo biloba combinations also showing promise. Curcumin and Melissa officinalis had limited effects, resveratrol showed mixed outcomes with some side effects, and matcha green tea may improve cognition and sleep quality. Despite generally favorable results, the studies varied considerably in design and quality; nonetheless, herbal extracts represent a prominent category of natural interventions in AD and MCI, underscoring the need for further large-scale, high-quality clinical trials to confirm their therapeutic potential. Full article

Spirulina and Chlorella are nutrient-rich microalgae widely consumed as dietary supplements; however, their high biosorption capacity raises concerns regarding the accumulation of environmental contaminants. This study analyzed 52 commercially available Spirulina and Chlorella products (29 conventional, 23 organic) to assess the co-occurrence of heavy metals and pharmaceutical residues, as these two classes of contaminants represent distinct yet complementary indicators of environmental pollution—heavy metals reflect long-term inputs from natural and industrial sources, while pharmaceuticals signal more recent contamination linked to human activity and wastewater discharge. To the best of our knowledge, this is the first study to investigate the presence of pharmaceutical residues—including cardiovascular drugs, antidepressants, antibiotics, and sulfonamides—in both conventional and organic formulations of microalgae-based dietary supplements. The analyses were performed using Inductively Coupled Plasma Mass Spectrometry and liquid chromatography coupled to tandem mass spectrometry. Aluminum, manganese, strontium, and zinc were the dominant trace elements. All samples complied with EU regulatory limits for toxic metals. More importantly, a wide range of pharmaceutical residues was detected in the supplements. Caffeine was the most frequently found compound, followed by metronidazole, carbamazepine, benzocaine, and tramadol. Particular concern is raised by the calculated TWI (% of tolerable weekly intake) for aluminum. Principal Component Analysis revealed significant compositional differences between Spirulina and Chlorella products, with vanadium notably elevated in conventionally cultivated Spirulina. Surprisingly, no significant differences were observed between organic and conventional products within each algal type. Our findings provide a novel contribution to the field by highlighting the presence of pharmaceutical residues in microalgae-based supplements and addressing a critical knowledge gap concerning potential chronic exposure to these contaminants through dietary intake. Full article

Optimizing nutrient formulations is essential to improving the biomass yield and C-phycocyanin (C-PC) productivity of Spirulina sp., a cyanobacterium with wide-ranging applications in food, pharmaceutical, and biotechnological industries. This study evaluated the effects of macronutrient modifications on growth and pigment biosynthesis using a two-level full factorial design across eight Zarrouk-based formulations compared to the standard medium. Cultivation experiments were conducted in triplicate, and growth was evaluated using linear growth rate, maximum optical density (OD₆₈₀), and dry biomass, while C-PC was quantified in crude extracts (PCL), dried biomass (PCD), and the purity index (PI). Among the tested formulations, F2 (16 g/L NaHCO₃, 5 g/L NaNO₃, 0.25 g/L K₂HPO₄) achieved the highest biomass productivity, yielding a 37.6% increase in dry weight and a 38.1% improvement in daily productivity compared to the control. In contrast, F3 (16 g/L NaHCO₃, 5 g/L NaNO₃, 1 g/L K₂HPO₄) yielded the highest C-PC content, nearly doubling both PCL and PCD values and enhancing pigment purity by 40.2%. ANOVA and interaction analyses confirmed that carbon and nitrogen synergistically promoted biomass formation, while phosphorus had a strong effect on pigment biosynthesis through C:N:P interactions. These findings demonstrate that Spirulina sp. requires distinct nutrient balances for optimal growth and pigment formation. Formulation F2 is ideal for maximizing biomass productivity, whereas F3 is optimal for high-value C-PC production. The results provide a rational framework for designing nutrient-efficient cultivation systems to advance sustainable Spirulina-based biomanufacturing. Full article

Objectives: The impact of combining animal and plant protein sources on digestibility is unclear, despite their increasing clinical use. Using a non-invasive dual stable isotope tracer approach, we assessed the digestibility, bioavailability and utilization of distinct protein blends in older adults, and associated plasma amino acid profiles and muscle protein synthesis (MPS) rates. Methods: Thirty-two older men (69 ± 3 y) consumed one of four protein blends (A (51:49, casein/soy); B and C (35:25:20:20, whey/casein/soy/pea); D (80:20, casein/whey)) alongside primed constant infusions of [1,2-¹³C₂] leucine for 8 h. Arterialized blood and vastus lateralis muscle biopsies were collected during a trickle feed protocol with all blends providing 20 g total protein, universally labeled ¹³C-spirulina, and ²H-cell free amino acid mix to determine digestibility. This trial was registered at Clinicaltrials.gov (ID-NCT07038655). Results: No differences (¹³C:²H ratios) were found in digestibility between the protein blends (p > 0.05). Mean (±SEM) fed state MPS at 2.5 h was 0.078 ± 0.009%/h, 0.075 ± 0.012%/h, 0.085 ± 0.007%/h and 0.065 ± 0.011%/h for drinks A, B, C and D, respectively, with a main time effect observed (p < 0.01), but no significant differences between drinks. Plasma essential amino acids (EAAs) increased significantly from baseline for all blends by 40 min (p < 0.05), with no differences between blends at any time point. Conclusions: These findings suggest that protein quantity (and/or leucine content), rather than composition, appears to be the most important factor driving MPS. Future work should focus on clinical populations where protein requirements and digestibility characteristics may differ. Full article

The search for sustainable development has led several production processes to adopt biorefineries. We evaluated the cultivation of Spirulina platensis and Scenedesmus obliquus in consortium (50/50%), with the addition of effluent of the anaerobic digestion (AD) of cattle waste, in fed-batch mode, to obtain biomass in 10 L raceways. Subsequently, cultivation was carried out at pilot scale in a 100 L raceway. Zarrouk medium (20%) was used, with the addition of 10% (v/v) of effluent in the fed-batch process. The biomasses were characterized to evaluate their application. In 10 L raceways, higher biomass concentrations were obtained in the cultivation of Spirulina with the addition of effluent, or with the microalgae consortia without the addition of effluent (around 1 g/L). The addition of the effluent reduced the carbohydrate content and increased the protein content during the cultivation. Scale-up (100 L raceways) with Spirulina showed similar results to those obtained in the 10 L raceways, with removals of 48%, 88% and 11% for COD, nitrogen and total phosphorus, respectively. The cultivation of microalgae in consortium and Spirulina can be used in the post-treatment of effluent of AD, allowing the production of biomass for different applications. Full article

Spirulina (Arthrospira platensis) is a protein- and antioxidant-rich microalga, but its use in beverages is limited by sensory acceptance. Four juices (apple, sour cherry, tomato, and celery) were initially tested with added blue and green spirulina (0.8% and 1.6% w/w). Based on preliminary acceptability scores, only sour cherry and tomato juices were selected for further analyses. Blue spirulina enrichment increased protein (from 0.80 to 1.36 g/100 g in sour cherry; 0.89 to 1.52 g/100 g in tomato), fat (0.05 to 0.21 g/100 g; 0.09 to 0.25 g/100 g), and energy (259 to 279 kJ/100 g; 140 to 170 kJ/100 g). Antioxidant activity improved significantly, with DPPH IC₅₀ reduced from 260 to 135 mg/mL (sour cherry) and from 268 to 171 mg/mL (tomato). Colour analysis confirmed a shift from red to blue hues (a* from 15.2 to 3.7 in sour cherry). Data were statistically processed using ANOVA followed by Tukey’s HSD test, while sensory data were additionally evaluated by PCA and GPA to identify product-specific differences. These results confirm that spirulina-enriched sour cherry and tomato juices are nutritionally enhanced functional beverages, though sensory off-notes remain a challenge for consumer acceptance. Full article

Phycocyanin (PC) is a type of alga-derived protein which exerts the role of light harvesting in Spirulina and Cyanophyta cells. Studies have widely proved that phycocyanin exhibits antineoplastic functions, while investigations on its bioactive peptides remain poorly documented. In previous work, three phycocyanin-derived peptides (PCPs: PCP1-3), which exerted anticancer effects in non-small cell lung cancer (NSCLC) cells, were successfully identified. In consideration of the in vitro instability of bioactive peptides, this study firstly investigated the stabilization and function of phycocyanin-derived peptides loaded by nanoparticles (NPs). Herein, Lipid-core NPs (PCPs@LEC–CS–PEG, diameter less than 100 nm) were prepared by interfacial deposition of a polymer using lecithin (LEC, liposome core shell), chitosan (CS, coating material) and polyethylene glycol (PEG, stabilizer). The results indicate that the embedding of LEC liposomes could significantly increase the stability of PCPs through promoting their resistance to high temperature (68.256 ± 3.26%), pH (60.17 ± 3.67%) and protease. Moreover, the modification of NPs by PEG and CS could enhance the protective effects on PCPs. Furthermore, in vitro phenotypic experiments confirmed that the inclusion of PCPs@PEG-CS–LEC NPs also significantly increased the inhibitory activities of PCPs against multiple NSCLC cells including A549, H1299 and LTEP-a2 cells, compared with non-embedded PCPs. The results of this work could lay a theoretical foundation for the further development and utilization of peptides derived from phycocyanin, and also for the investigation of the antineoplastic effects of bioactive peptides. Full article

Aging, one of the main factors associated with cardiovascular diseases, induces vascular modifications through nitric oxide (NO) release and oxidative stress. Based on the antioxidant properties of the non-enzymatic spirulina extract (non-Enz-Spir-E) and that degrading enzymes enhances the extract bioactivity, the aim of this study was to analyze the in vitro effect of an Alcalase-assisted Enz-Spir-E on the vasodilator function of conduit and resistance arteries (which differently contribute to blood pressure regulation) in aging. Therefore, thoracic aorta (TA) and mesenteric arteries (MA) from male Sprague–Dawley rats (20–22 months-old) were divided into two groups: non-incubated vessels and vessels exposed to Enz-Spir-E (0.1% w/v) for 3 h. The vasodilation to acetylcholine (ACh), sodium nitroprusside (SNP, a NO donor), carbon-monoxide-releasing molecule (CORM), and cromakalim (a potassium channel opener), as well as NO and superoxide anion production, were studied. Enz-Spir-E increased the ACh-, SNP-, and CORM-induced responses in both types of arteries, while the cromalakim-induced relaxation was increased only in MA. Enz-Spir-E increased NO release (TA: 5.69-fold; MA: 1.79-fold), while it reduced superoxide anion formation (TA: 0.52-fold; MA: 0.66-fold). These results indicate that Enz-Spir-E improves aging-associated vasodilation through increasing NO release/bioavailability in both types of arteries and hyperpolarizing mechanisms only in MA. Full article

Background: Chronic relapsing colitis involves immune dysregulation and oxidative stress, making monotherapies often insufficient. This study investigates a therapeutic strategy combining doxycycline (Dox), an immunomodulatory antibiotic, with Arthrospira platensis extracts to enhance anti-inflammatory and antioxidant effects, improving remission and controlling relapse. Methods: Ethanolic (ES) and aqueous (AS) extracts of A. platensis were chemically characterized by GC-MS after derivatization. Colitis was induced in mice using two intrarectal DNBS administrations spaced 7 days apart, with oral treatments (Dox, ES, AS, or combinations) given daily between doses. Disease progression was evaluated through clinical monitoring, histological scoring, and biochemical analysis, including MPO and CAT activities, as well as NO, MDA, and GSH levels. Results: GC-MS identified 16 bioactive compounds in each extract. ES contained mainly fatty acids and amino acids, whereas AS was rich in polysaccharides and phytol. Combined doxycycline and A. platensis extracts significantly enhanced recovery in reactivated DNBS colitis compared to monotherapies. Each treatment alone reduced disease severity, but their combination showed synergistic effects, significantly reducing disease activity index (p < 0.001), restoring mucosal integrity, and modulating inflammatory and oxidative markers (p < 0.001). Conclusion: Doxycycline potentiates the anti-colitic effects of A. platensis extracts via complementary mechanisms, offering a promising combination for managing relapsing colitis. Full article

This study optimizes the ultrasound-assisted extraction (UAE) of bioactive compounds from Spirulina platensis and develops a rapid, non-destructive analytical method. A Box–Behnken design and desirability function were used to find the optimal extraction conditions to simultaneously maximize total polyphenols, proteins, C-phycocyanin, and antioxidant activity. The optimal conditions were a solid-to-liquid ratio of 35 g/L, a time of 20 min, a pH of 10, and a temperature of 45 °C. Independent validation confirmed the model’s reliability, with experimental results closely matching predicted values. Furthermore, Near-Infrared (NIR) spectroscopy, combined with Artificial Neural Networks (ANNs), was explored as a predictive tool. The models, particularly those based on a semi-process NIR spectrometer, showed excellent predictive capabilities for key physicochemical properties, with an RPD of 3.9064 for L* and 2.8351 for TDS. This research establishes a highly reproducible and scalable extraction protocol, complemented by a fast and accurate analytical method, providing a significant advancement for the industrial application and quality control of Spirulina platensis extracts. Full article