Search Results (59)

Tyrosinase catalyzes the rate-limiting steps of melanin production, posing as an important target for treating skin pigmentation. This study investigates bioactive human tyrosinase inhibitors from Xanthium strumarium L. using a combined strategy of cell lysate, cell-based, and zebrafish assays. In this study, the methanol extract of Xanthium strumarium L. was identified as a potent inhibitor against tyrosinase in a cell lysate assay utilizing human MM418C1 melanoma cells. Subsequent phytochemical analysis resulted in the isolation of 11 natural products, including 4-hydroxybenzoic acid (4HB), three nucleotides, four caffeoylquinic acids and three alkaloids. Biological activity evaluation of isolated compounds suggested that 4HB was a potent inhibitor against tyrosinase with an IC₅₀ value of 59.5 μg/mL. Further evaluations revealed that 4HB significantly reduced the melanin content by 40% at the concentration of 500 mg/mL in human MM418C1 melanoma cells. 4HB activity was finally confirmed in vivo, by the demonstration of 40% reduction in melanin production in live zebrafish at the concentration of 15.63 μg/mL. Full article

The water-level fluctuation zone (WLFZ) of the Three Gorges Reservoir (TGR) experiences seasonal submergence and exposure, resulting in soil structure degradation and intensified erosion. This study investigated how flooding duration affects root development and the erosion resistance of root–soil complexes in the WLFZ of the TGR. Two representative herbaceous species were chosen for this study: Xanthium sibiricum, an annual with a taproot system, and Cynodon dactylon, a perennial with a fibrous root system. Root traits, soil erodibility K-value, shear strength, and soil texture were measured from plant and soil samples collected at different flooding durations (145–175 m elevations). Our results showed that prolonged flooding significantly suppressed root growth, particularly in the 145–155 m zone, where root length density and root tips were markedly reduced (p < 0.05). Soil erodibility increased with flooding duration, with erodibility K-values ranging from 0.050 ± 0.002 to 0.062 ± 0.001 t·hm²·h/(MJ·mm·hm²), while shear strength declined correspondingly. Textural shifts from silty loam to silt were observed at zones experiencing extended flooding, contributing to aggregate instability and decreased internal friction angles. Notably, Cynodon dactylon demonstrated superior soil reinforcement capacity compared to Xanthium sibiricum, with its root volume and surface area significantly correlated with reduced K-values (p < 0.01) and enhanced shear strength (p < 0.001), enabling it to better prevent bank erosion under flooding conditions. These findings underscore the importance of root morphological traits in maintaining soil stability under hydrological stress and highlight the potential of perennial fibrous-rooted species for vegetation-based erosion control in fine-textured riparian zones. This study provides a theoretical basis and practical reference for ecological restoration in the WLFZ of the TGR and similar environments. Full article

This study, which began in the 2013/2014 agricultural year, aimed to assess the suitability of two soil tillage systems for wheat cultivation: conventional soil tillage (CS), which involved moldboard plowing to a depth of 28 cm followed by a single pass with a rotary harrow to prepare the seedbed, and no-tillage (NT). It also sought to analyze the impacts of these systems on weed infestation levels and, consequently, on yield. A moderate level of fertilization was applied. The experimental field was established with a three-year crop rotation system: soybean–winter wheat–maize. The total number of weed species was 30 in CS, the representative species being Xanthium strumarium, and in NT there were 29 species, with Xanthium strumarium, Cirsium arvense, Bromus tectorum, and Agropyron repens predominating. There was an increase in the number of perennials (dicots and monocots). The total dry matter of weeds was 35.4 t ha⁻¹ in CS and 38.8 t ha⁻¹ in NT. After 11 agricultural years, it was found that there were no significant differences between the two soil tillage systems in terms of wheat yield (6.55 t ha⁻¹ in CS and 6.46 t ha⁻¹ in NT). The uneven rainfall negatively affected wheat growth and favored the spread of weeds, especially dicotyledonous ones. Full article

The United States leads in corn production and consumption in the world with an estimated USD 50 billion per year. There is a pressing need for the development of novel and efficient techniques aimed at enhancing the identification and eradication of weeds in a manner that is both environmentally sustainable and economically advantageous. Weed classification for autonomous agricultural robots is a challenging task for a single-camera-based system due to noise, vibration, and occlusion. To address this issue, we present a multi-camera-based system with decision-level sensor fusion to improve the limitations of a single-camera-based system in this paper. This study involves the utilization of a convolutional neural network (CNN) that was pre-trained on the ImageNet dataset. The CNN subsequently underwent re-training using a limited weed dataset to facilitate the classification of three distinct weed species: Xanthium strumarium (Common Cocklebur), Amaranthus retroflexus (Redroot Pigweed), and Ambrosia trifida (Giant Ragweed). These weed species are frequently encountered within corn fields. The test results showed that the re-trained VGG16 with a transfer-learning-based classifier exhibited acceptable accuracy (99% training, 97% validation, 94% testing accuracy) and inference time for weed classification from the video feed was suitable for real-time implementation. But the accuracy of CNN-based classification from video feed from a single camera was found to deteriorate due to noise, vibration, and partial occlusion of weeds. Test results from a single-camera video feed show that weed classification accuracy is not always accurate for the spray system of an agricultural robot (AgBot). To improve the accuracy of the weed classification system and to overcome the shortcomings of single-sensor-based classification from CNN, an improved Dempster–Shafer (DS)-based decision-level multi-sensor fusion algorithm was developed and implemented. The proposed algorithm offers improvement on the CNN-based weed classification when the weed is partially occluded. This algorithm can also detect if a sensor is faulty within an array of sensors and improves the overall classification accuracy by penalizing the evidence from a faulty sensor. Overall, the proposed fusion algorithm showed robust results in challenging scenarios, overcoming the limitations of a single-sensor-based system. Full article

Urolithiasis (UL) is the presence of stones in the kidneys or urinary tract; its prevalence has increased worldwide. Thus, strategies have been sought to reduce it and one of them is the use of medicinal plants due to their accessibility, low cost, and cultural traditions. Studies on traditional medicinal plants in UL mainly documented results of litholytic and urinary parameters. Although, stone formation is related to oxidative stress and inflammation, and only a few studies are focused on these types of biomarkers. Thus, the aim of the present review was to summarize studies showing the antioxidant and anti-inflammatory effects of traditional medicinal plants used in UL management. We performed a scoping review; the database sources used were MEDLINE/PubMed, Google Scholar, SpringerLink, Scielo and Redalyc. From a total of 184 studies screened, six were included from China (2), India (3), and Corea (1). These studies have shown the antioxidant and anti-inflammatory effects of traditional medicinal plants, including Glechoma longituba (G. longituba), Bergenia ligulate (B. ligulate), Lygodium japonicum (L. japonicum), Citrus limon (C. limon), Xanthium strumarium (X. strumarium) and Tribulus terrestris (T. terrestris). They have also described their molecular mechanism of antioxidant and anti-inflammatory effects through the activation of antioxidant genes induced by Nrf2 or by suppressing the inflammatory gene expression by the inhibition of NFκ-B. These effects could be modulated by their bioactive compounds, such as polyphenols, flavonoids, tannins, saponins, and terpenes, present in these plants. This review summarizes the antioxidant and anti-inflammatory effects of traditional medicinal plants and highlights their molecular mechanisms of action and main bioactive compounds. This evidence may be used in biotechnology and synthetic biology areas for the development of new products from plant-derived compounds to reduce the high recurrence rates of UL. Full article

Global changes, such as atmospheric nitrogen deposition, can facilitate alien plant invasions, which are often attributed to the increase in soil nitrogen availability. However, few studies have considered the effects of global change-driven alterations in soil nitrogen forms, especially under conditions with interspecific competition. In this study, we first determined the differences in growth, biomass allocation, and photosynthesis under different nitrogen forms and addition levels between three noxious invasive species (Xanthium strumarium, Ambrosia trifida, and Bidens frondosa) and their respective related natives grown with and without interspecific competition and then assessed the interspecific difference in nitrogen form preference using the ¹⁵N labeling technique. Interspecific competition significantly decreased the positive responses of growth to nitrogen addition for all three natives, while increasing the responses for all three invaders, particularly under nitrate addition. When grown in competition, all invaders showed significant growth advantages over their related natives in most cases, and responded more positively to the addition of nitrate relative to ammonium, while the natives responded more positively to ammonium addition. These findings indicate that the invaders prefer nitrate, while the natives prefer ammonium. Consistently, the growth advantages are more pronounced for the invaders under nitrate relative to ammonium addition, indicating that nitrate-rich habitats may be more vulnerable to the invaders. When grown in monoculture, however, the growth advantage of the invaders became smaller or even disappeared. Nitrogen form preference also disappeared in Siegesbeckia glabrescens (native) and Bidens frondosa (invasive). Interestingly, the native plant Xanthium sibiricum showed significantly higher total biomass than its invasive congener under ammonium addition in both mixed and monoculture conditions. Our ¹⁵N labeling experiment showed that all six species preferred nitrate over ammonium, although this was not significant for two natives (S. glabrescens and X. sibiricum), which is not completely consistent with the results from our nitrogen addition experiment. Our results indicate that global change-driven alterations in soil nitrogen forms, particularly the shift from ammonium to nitrate, may facilitate alien plant invasions. Planting patterns significantly affect the responses of invasive and native species to nitrogen forms and addition levels, with mixed-culture experiments providing better insights into the invasiveness of alien species. Full article

Heavy metal pollution poses a global environmental challenge, with lead (Pb) being particularly concerning due to its persistence and toxicity. This study investigated Xanthium strumarium L. from China’s Yellow River Sanmenxia section through hydroponic experiments (0–600 mg/L Pb²⁺, 1–11 d exposure) to elucidate its Pb²⁺ response mechanisms. Integrated analyses (EDX, FTIR, thermogravimetry, hyperspectral imaging) revealed a three-phase sequestration strategy: the roots immobilized 88.55% of Pb through pectin carboxyl de-esterification and lignin–Pb complexation, while the stems and leaves retained <11.14% and <0.31%, respectively. A critical threshold (300 mg/L) triggered nonlinear Pb accumulation escalation. Thermogravimetric analysis demonstrated enhanced cell wall stability under Pb stress (66.7% residual carbon increase at 600 mg/L). Hyperspectral features (1670 nm band intensity) effectively tracked physiological stress dynamics. The findings establish X. strumarium’s superior suitability for root-based immobilization rather than phytoextraction in Pb-contaminated sites, with its low translocation efficiency minimizing ecological risks. The identified concentration threshold and spectral biomarkers provide multi-scale insights for optimizing in situ phytostabilization strategies, advancing both theoretical understandings and practical applications in heavy metal remediation. Full article

The aim was to develop a series of handwashing gels containing a standardised extract of the aerial part of Gypsophila trichotoma as well as an essential oil from Xanthium strumarium fruits. The saponins present in the extract are a suitable alternative to synthetic sulphate surfactants and have better skin tolerance, and with the addition of essential oil, a higher antibacterial activity of the gels thus developed is achieved. The elaborated gels were characterised by good spreadability and a pH close to the physiological for healthy skin (pH 5.5), which is a prerequisite for improved skin tolerability. The dynamic rheological studies showed that the extract fraction increase was not associated with gel viscosity change, and it was only a function of the gelling agent concentration. The gels’ foam-forming ability increased with the increasing extract concentrations. The comparative evaluation of the antibacterial activity of elaborated gels vs. plain gel based on coco glucoside against a panel of selected pathogens demonstrated that the newly developed formulations have superior antibacterial effects. Based on the good technological characteristics and the results of antimicrobial testing of the washing gels thus developed, it can be concluded that they are promising candidates as personal hygiene products. Full article

Xanthium spinosum (X. spinosum) is a highly invasive weed native to South America and distributed in 17 provinces (municipalities) of China. It has severely negative influences on ecosystems, agriculture, and husbandry. However, few studies have reported on the impact of human activity and climate change on the future distribution and centroid shift of X. spinosum. This study aimed to investigate the potential geological distribution of X. spinosum in China, as well as the distribution pattern, centroid shift, and key environmental factors influencing its distribution, under four future climate scenarios (SSP1-26, SSP2-45, SSP3-70, and SSP5-85) based on the biomod2-integrated model. The results indicated that the suitable habitats for X. spinosum would expand in the future, mainly in Inner Mongolia, Northeast China, and the plateau regions (e.g., Xinjiang and Xizang). Under future climate scenarios, the centroid would shift toward the northwest or northeast part of China, with the SSP2-45-2050s scenario showing the maximum shift distance (161.990 km). Additionally, the key environmental variables influencing the distribution of X. spinosum, including human impact index, bio5, bio7, and bio12, were determined, revealing that most of them were related to human activities, temperature, and precipitation. This study enhances the understanding of the influence of human activity and climate change on the geographic range of X. spinosum. It provides references for early warning and management in the control of X. spinosum. Full article

This study examined a newly developed environmentally friendly plant-based corrosion inhibitor (Xanthium sibiricum). The natural potential method, linear polarization method, steel weight loss method, and corrosion area method were employed to verify the inhibitor’s effectiveness in chloride-containing concrete. The results indicated that Xanthium sibiricum elevated the natural potential of reinforcing steel in concrete, increased its self-corrosion potential, and reduced the self-corrosion current. After three months of curing, the corrosion rate of steel without an inhibitor was approximately 47.5% faster than the experimental group, with the steel loss rate about 40% more severe. The effectiveness of the inhibitor was influenced by increased chloride content in concrete. A two-dimensional multiphase ion transport model of reinforced concrete with realistic aggregate distribution was established using the finite element method (FEM). This model simulated chloride ion transport under typical civil engineering service environments—the coupled effects of a stray current and chloride environment. A predictive formula for the residual compressive strength of reinforced concrete was derived after corrosion under various voltages and chloride ion concentrations for a specific duration. In conjunction with a pump station project operating in a similar environment, the optimal dosage of the Xanthium sibiricum inhibitor for practical engineering was determined to be 2 g/L. At this dosage, the strength of reinforced concrete specimens increased by approximately 31.1%. Finally, a predictive formula for the residual compressive strength of reinforced concrete with an added inhibitor was obtained after corrosion under various voltages and chloride ion concentrations for a specific duration. The conclusions can enhance the durability and safety of concrete structures in similar projects, showing promising application prospects. Full article

Canary grass (Phalaris canariensis L.) is a versatile crop with global significance; it is primarily cultivated for its small elliptical seeds, which are used as bird feed and for human consumption. This crop is adapted to various climates and soils, so it can be grown successfully in Hungary. However, challenges such as weed control, climate change impacts, and soil factors require strategic management for sustained success in canary grass cultivation. Our study investigated the impact of management and environmental (as seasonal and soil) factors on pre-harvest weed vegetation in canary grass fields in Southeast Hungary between 2017 and 2020. In addition to showing the weed vegetation of the canary grass, the aim of our work was to promote more effective weed management of canary grass by revealing correlations between soil, seasonality, and management variables, influencing weed diversity and coverage. Using the analysis of covariance (ANCOVA) and correlation tests, we tested significant variables, providing insights into the complex interactions affecting weed composition. A redundancy analysis (RDA) further unveiled the relationships between explanatory variables and weed species’ composition. The findings offer valuable information for effective weed management strategies in canary grass cultivation. Our comprehensive study on canary grass fields in Southeast Hungary sheds light on significant factors influencing weed composition and abundance. The average weed coverage was 10.8%, with summer annuals and creeping perennials being the most prevalent life forms. Echinochloa crus-galli, Cirsium arvense, Xanthium italicum, and Setaria viridis were among the dominant species. ANCOVAs revealed the impact of soil, management, and seasonal factors on weed cover, species richness, diversity, and yield levels. Soil properties like texture, pH, and nitrogen content showed varying effects on weed parameters. The vintage effect, tillage systems, and farming practices also played crucial roles. The redundancy analysis highlighted the influence of the year, soil sulfur content, and winter preceding crops on weed composition. In conclusion, the herbaceous vegetation in the studied area is dominated by summer germinating and creeping perennial species. Despite slight differences in average coverage and occurrence, a well-defined set of significant species is evident. Multicollinearity among variables suggests limitations to further increase the number of variables that can be included in the analysis. The ANCOVAs showed that the soil, seasonal, and farming variables significantly influence overall weed vegetation and crop yield, with a lesser impact on species richness and diversity. The reduced RDA model highlights the strong influence of the year on species’ composition, emphasizing the inherent factors during canary grass cultivation that are challenging to modify through farming practices. Full article

The investigation into trade-offs among plant functional traits sheds light on how plants strategically balance growth and survival when facing environmental stress. This study sought to evaluate whether trade-offs observed at both community and individual species levels could indicate adaptive fitness across an intensity of flooding intensity. The study was conducted at 25 sampling sites spanning approximately 600 km along the riparian zone in the Three Gorges Reservoir area, China. The findings revealed that, along the flooding gradient, the overall riparian community did not exhibit significant trade-offs between leaf and root traits. Examining three broadly distributed dominant species (Cynodon dactylon, Xanthium strumarium, and Abutilon theophrasti), perennial plants showed pronounced trade-offs under low flooding intensity, while annuals exhibited trade-offs under moderate and low flooding intensity. The trade-offs were evident in traits related to nitrogen-carbon resources, such as specific leaf area, root tissue density, and photosynthetic rate. However, under strong flooding intensity, the relationship between leaf and root traits of the species studied was decoupled. Furthermore, the study identified a significant correlation between soil nitrogen and the trade-off traits under moderate and low flooding intensity. Integrating results from the CSR (Competitors, Stress-tolerators, Ruderals) strategy model, species niche breath analysis, and nitrogen-regulated trade-off, the study revealed that, in the face of high flooding intensity, perennial species (C. dactylon) adopts an S-strategy, demonstrating tolerance through a conservative resource allocation that decouples leaf-root coordination. Annual species (X. strumarium and A. theophrasti), on the other hand, exhibit niche specialization along the flooding gradient, employing distinct strategies (R- and C-strategy). As flooding stress diminishes and soil nitrogen level decreases, plant strategies tend to shift towards an R-strategy with a competition for reduced N resources. In conclusion, the study highlighted the pivotal roles of soil nitrogen and flooding intensity acting as the dual determinants of species growth and tolerance. These dynamics of growth-tolerance balance were evident in the diverse trade-offs between leaf and root traits of individual plant species with different life histories, underscoring the array of adaptive strategies employed by riparian plants across the flooding intensity gradient. Full article

The competitive ability of invasive plants is a key factor in their successful invasion, and research on this ability of invasive plants can provide a theoretical basis for the prevention and control of invasive plants. This study used Cuscuta australis, Xanthium spinosum, and Xanthium italicum as research materials and conducted outdoor controlled pot experiments to compare and study the changes in the biomass, competitiveness, and growth cycle of X. spinosum and X. italicum parasitized by C. australis at different growth stages. The results showed that (1) parasitism by C. australis increased the biomass of X. spinosum and decreased that of X. italicum, but under parasitism, the root cap ratio of X. spinosum and X. italicum increased, and the fruit biomass ratio decreased, indicating that X. spinosum and X. italicum reduced the energy input for reproduction and increased the energy input for nutrient growth to resist the impact of C. australis parasitism; (2) the relative competitive intensity calculated based on the total biomass of a single plant showed a negative value for X. spinosum during parasitism at the flowering and fruit stages, indicating an increase in competitive ability, and X. italicum showed a positive value during parasitism at the seedling and flowering stages, indicating a decrease in competitive ability; and (3) the parasitism of C. australis significantly shortened the fruit stage of X. spinosum and X. italicum, leading to a significant advance in their flowering, fruiting, and fruit ripening times. Simultaneously, it significantly reduced the morphological indicators of biomass, plant height, and crown width. Thus, C. australis parasitism has a certain inhibitory effect on the competitive ability of some invasive plants and can shorten their growth cycle, the latter of which has an important impact on their reproduction and diffusion. Full article

Studying the effects of different degrees of exotic plant invasion on native plants’ community structure and plant diversity is essential for evaluating the harm caused to ecosystems by plant invasion. In this study, we investigated the effects of Xanthium spinosum, a widespread invasive species, on plant community species diversity and community stability in the Ili River Valley area of Xinjiang, China, under three invasion levels (no invasion and low, moderate, and heavy invasion), and the competitive advantage index, invasion intensity, and contribution of plant community species diversity to community stability and invasibility were determined for the prickly fungus under different degrees of invasion. The results show that there were significant differences (p < 0.05) in the species diversity and community stability of plant communities caused by different degrees of invasion of X. spinosum. The species diversity and stability of plant communities were negatively correlated with the community invasibility, competitive advantage, and invasion intensity of X. spinosum (p < 0.05); therefore, the competitive advantage and invasion intensity of X. spinosum increase with the increase of its invasion degree. On the contrary, community species diversity and stability decreased with the increase of its invasion degree, ultimately leading to differences in community invasibility under different invasion degrees. The Shannon–Wiener and Simpson’s indices were the greatest contributors to community stability and invasibility, respectively. Moderate and heavy levels of invasion by X. spinosum reduced the diversity and stability of local plant communities, increased the invasibility of communities, and substantially affected the structures of plant communities. Therefore, the continued invasion by X. spinosum will have an immeasurable impact on the fragile ecosystems and diversity of indigenous species in Xinjiang. We recommend that this invasive species be controlled and eradicated at the early stages of invasion to prevent further harm. Full article

Seventy percent of the territory of Uzbekistan consists of arid regions. This situation is considered very favorable for plants adapted to a desert climate. Medicinal plants distributed in the arid regions of Uzbekistan have not been studied much. Medicinal plants are considered inexpensive, yet are vital for the lives of local residents. They play a very important role in the traditional healing of ailments. To determine the current state of medicinal plants and enhance their subsequent protection and sustainable use, it is necessary to obtain annual information on the state of their distribution, their population size, and the impact of negative factors on their populations. Based on our field studies, which were conducted during the period from 2012 to 2022 in the arid regions of Uzbekistan, we updated the checklists of the main medicinal plants used in these regions. A total of 529 medicinal species belonging to 70 families and 269 genera were identified in the study region. Several species, including Peganum harmala L., Capparis spinosa L., Ferula foetida (Bunge) Regel, Glycyrrhiza glabra L., Alhagi pseudalhagi (M. Bieb.) Desv. ex Wangerin, Lagochilus inebrians Bunge, Xanthium strumarium L., Silybum marianum (L.) Gaertn., Onopordum acanthium L., Ziziphora tenuior L., and Cichorium intybus L., are spread over large areas and have been used regularly by the locals since ancient times. These species are common in saline and degraded soils in arid regions of Uzbekistan. Semi-structured interviews were conducted with tabibs (traditional doctors), elders, herders, and residents with experience in traditional healing using medicinal plants. The medicinal value of most plants was based on the interviews with representatives of the local population, which were useful for understanding traditional healing skills and customer service skills. Full article