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10 pages, 213 KB  
Article
Motivational Determinants of Recreational Padel Participation: A Comparative Analysis Across Age and Gender
by Zlatan Bilić, Petar Barbaros, Vedran Dukarić, Tea Lovreković and Lidija Petrinović
Sports 2025, 13(11), 377; https://doi.org/10.3390/sports13110377 - 3 Nov 2025
Viewed by 259
Abstract
The aim of this study is to examine the differences in motivation when playing recreational padel regarding gender and age. The sample included 201 respondents (M = 131, F = 70) with an average age of 31 ± 7.07 years. Data was collected [...] Read more.
The aim of this study is to examine the differences in motivation when playing recreational padel regarding gender and age. The sample included 201 respondents (M = 131, F = 70) with an average age of 31 ± 7.07 years. Data was collected online via the Sport Motivation Scale-II (SMS-II) questionnaire. Spearman correlation presented the highest correlation between intrinsic and introjected (r = 0.658) motivation forms. Furthermore, results were analyzed using the Mann–Whitney U test. The results showed a statistically significant difference in motivation between men and women exclusively in the dimension of introjected regulation (M = 107.54, F = 88.76; p = 0.03), while no difference was found in the other dimensions. Also, introjected regulation was found to have the highest impact on differences across age (18–30 yr = 94.39; 31–50 yr = 110.59; p = 0.05). The research shows that padel as a recreational activity has a similar motivational effect on different age and gender groups, apart from introjected regulation, where men show higher results. Overall, the motivation to engage in padel largely depends on personal interests, enjoyment and perceived benefits, while gender and age have a limited influence, which coincides with some of the previous literature. Since research on motivation in the context of padel is still rare, this research contributes to the understanding of the factors that influence recreational engagement in this sport. Full article
19 pages, 2098 KB  
Article
Soil Carbon Dynamics, Sequestration Potential, and Physical Characteristics Under Grazing Management in Regenerative Organic Agroecosystems
by Said A. Hamido, Arash Ghalehgolabbehbahani and Andrew Smith
Agronomy 2025, 15(10), 2426; https://doi.org/10.3390/agronomy15102426 - 20 Oct 2025
Viewed by 802
Abstract
Rotational grazing and cover crops are conservation practices known to improve soil health, particularly soil organic carbon (SOC) and aggregate stability. Combining both practices may enhance these benefits more than either alone. With grazing lands covering 41% of U.S. agricultural land, adopting such [...] Read more.
Rotational grazing and cover crops are conservation practices known to improve soil health, particularly soil organic carbon (SOC) and aggregate stability. Combining both practices may enhance these benefits more than either alone. With grazing lands covering 41% of U.S. agricultural land, adopting such methods could significantly impact the soil carbon cycle. A study near Koshkonong, Missouri, examined the effects of regenerative organic grazing with Bubalus bubalis (Linnaeus) on SOC, carbon sequestration, aggregate stability, and soil resistance. The 1620-hectare ranch tested four treatments: rotational grazing with cover crops (RGCC), grazing on native grasses (RGNCC), cover crops without grazing (NGCC), and orchards without cover crops or grazing (NGNCC). Cover crops were seeded twice yearly with diverse species. After three years, SOC increased most in NGNCC (28%), followed by RGCC (13%), NGCC (7%), and RGNCC (4%). Annual carbon gains in surface soils were highest in NGNCC (0.99 Mg ha−1 yr−1). Across all depths, NGCC led (4.88 Mg ha−1 yr−1). Aggregate stability was greatest in non-grazed systems, particularly in fine aggregates, and declined with soil disturbance. Overall, low-disturbance systems like orchards and no-grazing cover crop plots enhanced soil structure and carbon storage. Strategic management is key to improving soil function and ecosystem resilience. Full article
(This article belongs to the Special Issue Soil Health to Human Health)
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15 pages, 1275 KB  
Article
Sex Differences in the High Jump Kinematics of U18 Adolescent Athletes
by Vassilios Panoutsakopoulos
Appl. Sci. 2025, 15(17), 9382; https://doi.org/10.3390/app15179382 - 26 Aug 2025
Viewed by 1173
Abstract
Biomechanical analyses of technique are essential for performance improvement in athletic jumps, but scarce evidence exists for adolescent athletes in the literature. The purpose of this study was to examine the sex differences in the high jump biomechanics of U18 athletes. Twenty-one women [...] Read more.
Biomechanical analyses of technique are essential for performance improvement in athletic jumps, but scarce evidence exists for adolescent athletes in the literature. The purpose of this study was to examine the sex differences in the high jump biomechanics of U18 athletes. Twenty-one women (15.2 ± 1.0 yrs) and twenty-one men (15.2 ± 1.2 yrs) Greek U18 high jumpers were recorded in regional competitions using two cameras (sampling frequency: 60 fps). The kinematic parameters of the last step, the take-off, and the crossbar clearance were calculated using 3D-DLT analysis. The independent samples t-test was used to detect significant (p < 0.05) differences between groups. Results revealed that men had significantly (p < 0.05) better performance, with larger last step angle and length, body center of mass (BCM) height at the final touchdown and take-off, approach and vertical BCM take-off velocity, touchdown lateral inclination of the take-off leg, and swing leg knee angle at take-off. Women had significantly (p < 0.05) higher vertical BCM velocity at touchdown. No differences (p > 0.05) were observed for take-off angle, the support leg knee angle, the inclination of the torso at touchdown, or the vertical BCM displacement during the take-off phase. The anthropometric and physical conditioning differences between sexes contributed to the findings of the study. Coaches should consider the age and sex differences of adolescent athletes when designing training programs to optimize high jump performance. Full article
(This article belongs to the Special Issue Advances in Sports Science and Biomechanics)
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15 pages, 682 KB  
Article
Does Exercise Performance Differ Among Male Law Enforcement Officers Based on Their Body Mass Index Category?
by Drew E. Gonzalez, Don R. Melrose, Filip Kukić, Robert G. Lockie, Robin M. Orr and J. Jay Dawes
Healthcare 2025, 13(13), 1584; https://doi.org/10.3390/healthcare13131584 - 2 Jul 2025
Cited by 1 | Viewed by 763
Abstract
Objectives: To assess differences in exercise performance among law enforcement officers (LEOs) based on body mass index (BMI). Methods: Five hundred and thirty-two male LEOs (n = 532; age 38.9 ± 7.4 yrs; height: 180.1 ± 6.9 cm; body mass: 92.1 ± [...] Read more.
Objectives: To assess differences in exercise performance among law enforcement officers (LEOs) based on body mass index (BMI). Methods: Five hundred and thirty-two male LEOs (n = 532; age 38.9 ± 7.4 yrs; height: 180.1 ± 6.9 cm; body mass: 92.1 ± 15.1 kg) were analyzed. The LEOs were stratified into three BMI groups: “healthy” (18.5–24.9 kg/m2), “overweight” (25.0–29.9 kg/m2), and “obese” (≥30.0 kg/m2). Tests for push-ups, sit-ups, estimated VO2max, vertical jump (VJ), and peak anaerobic power output (PAPw) were conducted. Non-parametric Kruskal–Wallis H and Quade’s rank-based ANCOVA with age as a covariate test were used to evaluate differences in exercise performance between BMI groups. Mann–Whitney U tests with Bonferroni post hoc corrections were used for pairwise comparisons. Effect sizes were calculated as rank eta squared (ηH2) for the Kruskal–Wallis H test results. Results: Differences were noted across BMI groups for the push-ups (p < 0.001, ηH2 = 0.101), sit-ups (p < 0.001, ηH2 = 0.187), VO2max (p < 0.001, ηH2 = 0.145), VJ (p < 0.001, ηH2 = 0.137), and PAPw (p < 0.001, ηH2 = 0.504). The pairwise comparisons revealed differences between each group, with the obese and overweight groups exhibiting a lower VJ, VO2max, and performance in push-ups and sit-ups while having a higher PAPw than the healthy group, even after adjusting for age. Conclusions: These data demonstrate that a higher BMI is associated with poorer exercise performance, except for PAPw, and highlight the importance of maintaining a healthy BMI in LEOs. Full article
(This article belongs to the Special Issue Health and Readiness of Tactical Populations)
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12 pages, 2409 KB  
Review
Challenge at the Edge: Long-Term Sea Level Rise vs. Short-Term Extreme Events
by Gary B. Griggs
J. Mar. Sci. Eng. 2025, 13(6), 1123; https://doi.org/10.3390/jmse13061123 - 4 Jun 2025
Viewed by 1285
Abstract
California and most other coastlines around the nation and the world are being impacted by both long-term sea-level rise (SLR) and short-term extreme events. Global sea level over the last 10 years of satellite altimetry has averaged approximately 4.1 mm/yr. (~16 in./100 yrs.), [...] Read more.
California and most other coastlines around the nation and the world are being impacted by both long-term sea-level rise (SLR) and short-term extreme events. Global sea level over the last 10 years of satellite altimetry has averaged approximately 4.1 mm/yr. (~16 in./100 yrs.), although this rate is accelerating at about 1.2 mm/yr. per decade. Projections of future sea levels have now been developed by many different agencies, organizations, and committees, and cluster around 12 inches by 2050. Over the near term, however, until mid-century, and likely beyond, it will be the short-term extreme events such as hurricanes along the U.S. Atlantic and Gulf coasts, and the coincidence of very large waves and high astronomic tides along the U.S. Pacific coasts that will pose the major threat to both public infrastructure and private development. Full article
(This article belongs to the Section Coastal Engineering)
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9 pages, 461 KB  
Article
Assessment of Youth Water Polo Players’ Swimming Sprint Potential: A New Approach to Building an International Model
by Andrea Perazzetti, Antonio Tessitore, Mehmet Zeki Özkol, Nebojša Novoselac and Milivoj Dopsaj
J. Funct. Morphol. Kinesiol. 2025, 10(2), 200; https://doi.org/10.3390/jfmk10020200 - 31 May 2025
Cited by 2 | Viewed by 943
Abstract
Background: To cope with their horizontal swimming phases, water polo players use different swimming techniques, such as specific variants of the crawl swimming style. Thus, this study aimed to investigate the swimming skills of young water polo players. Methods: An all-out [...] Read more.
Background: To cope with their horizontal swimming phases, water polo players use different swimming techniques, such as specific variants of the crawl swimming style. Thus, this study aimed to investigate the swimming skills of young water polo players. Methods: An all-out 25-m sprint swimming test in crawl style was completed by 273 international youth water polo players (age = 14.0 ± 0.8 yrs) in two modalities: basic crawl with the head in the water (25CHeadIN), and a crawl performed while dribbling the ball (25CBall). Results: We registered an average time of 14.79 and 15.64 s for 25CHeadIN and 25CBall, respectively, in which the ball dribbling increased to 5% of the swimming time. A swimming skill index (25CSIC) was calculated to account for differences in ball dribbling speeds, which, considering our international sample and in the absence of previous data, we could speculate as the first international standard value for 14-year-old male water polo players competing at international level. The averaged values for 25CSI and 25CSIC were 0.94 ± 0.04 (a.u.) and 1.52 ± 0.15 (a.u.), respectively. Factor analysis indicated that swimming with and without the ball are structurally distinct technical skills, highlighting the specificity of these water polo players’ abilities. Moreover, the study shows significant differences (p < 0.05) between players from different countries and despite some limitations, its results provide valuable insights for the assessment and development of sprint swimming skills in youth water polo players. Conclusions: In summary, the findings of this research provide practical implications for training, player selection, player development and the optimization of youth water polo player performance. Full article
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19 pages, 27219 KB  
Article
A Method for Assessing the Potential of Multifunctional Retrofitting of Rural Roofs Based on GF-2 Remote Sensing Imagery
by Junqi Wang, Linlin Cheng, Yang Zheng, Huizhen Cui and Mengyao Zhu
Sensors 2025, 25(3), 770; https://doi.org/10.3390/s25030770 - 27 Jan 2025
Viewed by 1278
Abstract
Green roofs and photovoltaic (PV) roofs are important forms of roof retrofitting, and unused rural roofs provide favorable conditions for the development of green roofs and PV roofs. Here, this study proposes a new method for assessing the potential of multifunctional retrofitting of [...] Read more.
Green roofs and photovoltaic (PV) roofs are important forms of roof retrofitting, and unused rural roofs provide favorable conditions for the development of green roofs and PV roofs. Here, this study proposes a new method for assessing the potential of multifunctional retrofitting of rural roofs. Firstly, rural roof types were classified into three categories based on GF-2 imagery: flat roofs, east–west pitched roofs, and north–south pitched roofs. The roof types were extracted based on the revised U-Net model, which aims to enhance the extracted features of the buildings and improve the perception of the buildings. Secondly, three types of retrofits—PV roofs, green roofs, and PV-green roofs—were designed taking into account the type, orientation, and area of the roofs. Finally, the potential electricity and carbon benefits of the different retrofit types of roofs were calculated separately, with the aim of realizing an assessment of the potential for roof retrofitting in the rural areas of Mentougou, Beijing. The results of the study showed that 35,407 (281.97 ha) roofs could be used for multifunctional retrofitting. If rural roofs are retrofitted with multifunctionality according to the methodology of this paper, they can absorb an additional 4.66 × 104 kg/yr of CO2 and increase biomass production by 0.99 × 104 kg/yr compared to retrofitting only PV roofs, and they can generate an additional 34.1 GWh/yr of electricity and reduce CO2 emissions by an additional 3.3 × 107 kg/yr compared to retrofitting to both PV roofs and green roofs. The assessment methodology of this study provides decision makers with data references on the multifunctional potential of rural rooftops for retrofitting, which can optimize the use of rural rooftops, and at the same time is important for promoting the energy transition in rural areas. Full article
(This article belongs to the Special Issue Recent Advances in Synthetic Aperture Radar (SAR) Remote Sensing)
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22 pages, 1660 KB  
Article
Evaluating the Environmental Impact of Heat Pump Systems: An Integrated Approach to Sustainable Building Operations
by Mahdiyeh Zafaranchi, William T. Riddell, Nicholas B. Chan, Elizabeth Saliba and Luke Leung
Energies 2025, 18(2), 388; https://doi.org/10.3390/en18020388 - 17 Jan 2025
Cited by 2 | Viewed by 3154
Abstract
This study evaluates the energy consumption and embodied carbon emissions of various heat pump systems for an office building in Chicago, IL, U.S., over a 50-year lifespan, including the operation, manufacturing, and construction phases. The analyzed systems include air source heat pumps (ASHP) [...] Read more.
This study evaluates the energy consumption and embodied carbon emissions of various heat pump systems for an office building in Chicago, IL, U.S., over a 50-year lifespan, including the operation, manufacturing, and construction phases. The analyzed systems include air source heat pumps (ASHP) in Air to Air and Air to Water configurations, and ground source heat pumps (GSHP) in Soil to Air and Soil to Water configurations. A traditional HVAC system serves as the baseline for comparison. Advanced simulation tools, including Rhino, Grasshopper, TRACE 700, and One Click LCA, were used to identify the optimal HVAC system for sustainable building operations. Unlike prior studies focusing on GSHP versus traditional HVAC systems, this research directly compares GSHP and ASHP configurations, addressing a significant gap in the sustainable HVAC system design literature. The GSHP (Soil to Water) system demonstrated the lowest energy intensity at 100.8 kWh/m2·yr, a 41.8% improvement over the baseline, and the lowest total embodied carbon emissions at 3,882,164 kg CO2e. In contrast, the ASHP (Air to Air) system, while reducing energy consumption relative to the baseline, exhibited the highest embodied carbon emissions among the heat pump configurations due to its higher operational energy demands. The study highlights the significance of the operating phase in embodied carbon contributions. These findings emphasize the importance of a holistic design approach that considers both operational and embodied impacts to achieve sustainable building designs. Full article
(This article belongs to the Special Issue Sustainable Energy, Environment and Low-Carbon Development)
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17 pages, 4041 KB  
Article
Sources and Trends of CO, O3, and Aerosols at the Mount Bachelor Observatory (2004–2022)
by Noah Bernays, Jakob Johnson and Daniel Jaffe
Atmosphere 2025, 16(1), 85; https://doi.org/10.3390/atmos16010085 - 15 Jan 2025
Viewed by 1021
Abstract
Understanding baseline O3 is important as it defines the fraction of O3 coming from global sources and not subject to local control. We report the occurrence and sources of high baseline ozone days, defined as a day where the daily maximum [...] Read more.
Understanding baseline O3 is important as it defines the fraction of O3 coming from global sources and not subject to local control. We report the occurrence and sources of high baseline ozone days, defined as a day where the daily maximum 8 h average (MDA8) exceeds 70 ppb, as observed at the Mount Bachelor Observatory (MBO, 2.8 km asl) in Central Oregon from 2004 to 2022. We used various indicators and enhancement ratios to categorize each high-O3 day: carbon monoxide (CO), aerosol scattering, the water vapor mixing ratio (WV), the aerosol scattering-to-CO ratio, backward trajectories, and the NOAA Hazard Mapping System Fire and Smoke maps. Using these, we identified four causes of high-O3 days at the MBO: Upper Troposphere/Lower Stratosphere intrusions (UTLS), Asian long-range transport (ALRT), a mixed UTLS/ALRT category, and events enhanced by wildfire emissions. Wildfire sources were further divided into two categories: smoke transported in the boundary layer to the MBO and smoke transported in the free troposphere from more distant fires. Over the 19-year period, 167 high-ozone days were identified, with an increasing fraction due to contributions from wildfire emissions and a decreasing fraction of ALRT events. We further evaluated trends in the O3 and CO data distributions by season. For O3, we found an overall increase in the mean and median values of 2.2 and 1.5 ppb, respectively, from the earliest part of the record (2004–2013) compared to the later part (2014–2022), but no significant linear trends in any season. For CO, we found a significant positive trend in the summer 95th percentiles, associated with increasing fires in the Western U.S., and a strong negative trend in the springtime values at all percentiles (1.6% yr−1 for 50th percentile). This decline was likely associated with decreasing emissions from East Asia. Overall, our findings are consistent with the positive trend in wildfires in the Western United States and the efforts in Asia to decrease emissions. This work demonstrates the changing influence of these two source categories on global background O3 and CO. Full article
(This article belongs to the Special Issue Measurement and Variability of Atmospheric Ozone)
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14 pages, 1478 KB  
Article
Stress-Related Hormonal and Psychological Changes to Simulated and Official Judo Black Belt Examination in Older Tori and Adult Uke: An Exploratory Observational Study
by Simone Ciaccioni, Francesca Martusciello, Andrea Di Credico, Flavia Guidotti, Daniele Conte, Federico Palumbo, Laura Capranica and Angela Di Baldassarre
Sports 2024, 12(11), 310; https://doi.org/10.3390/sports12110310 - 14 Nov 2024
Cited by 4 | Viewed by 1846
Abstract
This study investigated the psycho-physiological impact of a black belt examination. Older brown-belt judoka (Tori, F = 2, M = 4; age = 75.6 ± 4.5 yrs) and their 2nd–5th Dan black-belt coaches (Uke; M = 6; age = 36.5 ± 10.8 yr) [...] Read more.
This study investigated the psycho-physiological impact of a black belt examination. Older brown-belt judoka (Tori, F = 2, M = 4; age = 75.6 ± 4.5 yrs) and their 2nd–5th Dan black-belt coaches (Uke; M = 6; age = 36.5 ± 10.8 yr) were evaluated during a simulated and official examination and a resting day. Participants’ trait anxiety (STAI-Y2) was recorded prior to the study. State anxiety (STAI-Y1), ratings of perceived exertion (RPE), enjoyment (ENJ), and fear of falling (FoF) were collected 15 min before and after the experimental conditions. Saliva samplings at awakening (T0), PRE (T1), and POST (T2) exercise and during the recovery (15 min-T3, 30 min-T4, 60 min-T5) were collected for cortisol (sC), testosterone (sT), and alpha-amylase (sAA). Participants showed normal age-reference population trait anxiety. A difference (p ≤ 0.05) for role emerged for ENJ and sT only. For STAI-Y1, higher PRE values with respect to POST ones emerged (p = 0.005), and the highest values (p = 0.007) for PRE of the examination were with respect to the simulation. For sAA, differences for sampling were found in the examination conditions only, with peak values at T2 (370.3 ± 78.6 U/mL, p = 0.001). For sC, a significant peak value (0.51 ± 0.09 μg/dL, p = 0.012) emerged at T2 in the examination condition. With respect to Tori, Uke showed higher mean sT values in all conditions (p ≤ 0.05) and the highest T2 during examination (712.5 ± 57.2 pg/mL). Findings suggest the relevance of monitoring psycho-physiological stress-related responses in judo for optimizing both coaching effectiveness and sport performance, especially in older judo practitioners. Full article
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28 pages, 32933 KB  
Article
The Change Detection of Mangrove Forests Using Deep Learning with Medium-Resolution Satellite Imagery: A Case Study of Wunbaik Mangrove Forest in Myanmar
by Kyaw Soe Win and Jun Sasaki
Remote Sens. 2024, 16(21), 4077; https://doi.org/10.3390/rs16214077 - 31 Oct 2024
Cited by 4 | Viewed by 4630
Abstract
This paper presents the development of a U-Net model using four basic optical bands and SRTM data to analyze changes in mangrove forests from 1990 to 2024, with an emphasis on the impact of restoration programs. The model, which employed supervised learning for [...] Read more.
This paper presents the development of a U-Net model using four basic optical bands and SRTM data to analyze changes in mangrove forests from 1990 to 2024, with an emphasis on the impact of restoration programs. The model, which employed supervised learning for binary classification by fusing multi-temporal Landsat 8 and Sentinel-2 imagery, achieved a superior accuracy of 99.73% for the 2020 image classification. It was applied to predict the long-term mangrove maps in Wunbaik Mangrove Forest (WMF) and to detect the changes at five-year intervals. The change detection results revealed significant changes in the mangrove forests, with 29.3% deforestation, 5.75% reforestation, and −224.52 ha/yr of annual rate of changes over 34 years. The large areas of mangrove forests have increased since 2010, primarily due to naturally recovered and artificially planted mangroves. Approximately 30% of the increased mangroves from 2015 to 2024 were attributed to mangrove plantations implemented by the government. This study contributes to developing a deep learning model with multi-temporal and multi-source imagery for long-term mangrove monitoring by providing accurate performance and valuable information for effective conservation strategies and restoration programs. Full article
(This article belongs to the Special Issue Remote Sensing in Mangroves III)
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17 pages, 5955 KB  
Article
Effects of Wildfire Smoke on Volatile Organic Compound (VOC) and PM2.5 Composition in a United States Intermountain Western Valley and Estimation of Human Health Risk
by Damien T. Ketcherside, Dylan D. Miller, Dalynn R. Kenerson, Phillip S. Scott, John P. Andrew, Melanie A. Y. Bakker, Brandi A. Bundy, Brian K. Grimm, Jiahong Li, Laurel A. Nuñez, Dorian L. Pittman, Reece P. Uhlorn and Nancy A. C. Johnston
Atmosphere 2024, 15(10), 1172; https://doi.org/10.3390/atmos15101172 - 30 Sep 2024
Cited by 3 | Viewed by 4235
Abstract
With a warmer and drier climate, there has been an increase in wildfire events in the Northwest U.S., posing a potential health risk to downwind communities. The Lewis–Clark Valley (LCV), a small metropolitan area on the Washington/Idaho border in the United States Intermountain [...] Read more.
With a warmer and drier climate, there has been an increase in wildfire events in the Northwest U.S., posing a potential health risk to downwind communities. The Lewis–Clark Valley (LCV), a small metropolitan area on the Washington/Idaho border in the United States Intermountain West region, was studied over the time period of 2017–2018. The main objective was to determine the community’s exposure to particulate matter (PM2.5) and volatile organic compounds (VOCs) during wildfire smoke events and to estimate the associated health risk. VOCs were analyzed previously in the LCV using sorbent tube sampling and thermal-desorption gas-chromatography mass-spectrometry (TD-GC-MS) during several local smoke events in the 2017–2018 fire seasons. PM2.5 measurements were obtained from nearby agency monitors. PM2.5 reached up to 200 µg/m3 in 2017 and over 100 µg/m3 in 2018 in the LCV, and has been observed to be increasing at a rate of 0.10 µg m−3/yr over the past two decades. Benzene, a carcinogen and air toxic, was measured with concentrations up to 11 µg/m3, over ten times the normal level in some instances, in the LCV. The health risk in the LCV from benzene was calculated at seven extra cancers per million for lifetime exposure and thirteen extra cancers per million considering all air toxics measured. The other cities monitored showed similar lifetime cancer risk, due to benzene of about 6–7 extra cancers per million. This work is important, as it measures ground-level exposures of VOCs and demonstrates decreases in PM2.5 air quality over time in the region. Full article
(This article belongs to the Special Issue Outdoor Air Pollution and Human Health (3rd Edition))
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14 pages, 408 KB  
Article
Energy Deficit and Factors Associated with Energy Balance during a Combat Deployment in U.S. Army Special Operation Forces Soldiers
by Evan G. Tryon, Nicholas D. Barringer, Harris R. Lieberman and William R. Conkright
Nutrients 2024, 16(18), 3072; https://doi.org/10.3390/nu16183072 - 12 Sep 2024
Cited by 1 | Viewed by 2468
Abstract
The purpose of this study was to determine the difference between estimated energy expenditure (EE) and self-reported dietary intake (EI), and factors associated with energy balance in deployed U.S. Army Special Operations Forces (SOF) Soldiers. Methods: Forty-six SOF Soldiers (age: 30.1 ± 3.5 [...] Read more.
The purpose of this study was to determine the difference between estimated energy expenditure (EE) and self-reported dietary intake (EI), and factors associated with energy balance in deployed U.S. Army Special Operations Forces (SOF) Soldiers. Methods: Forty-six SOF Soldiers (age: 30.1 ± 3.5 yrs, body mass index: 27.7 ± 4.1 kg/m2) completed surveys on demographic data, mission activity characteristics, gastrointestinal issues, ration consumption, resilience, mood state, and dietary intake using a 127-question food frequency questionnaire at the end of a six-month deployment. EE was estimated using a SOF-specific prediction equation with a physical activity factor of 2.1. A paired t-test compared reported energy intake (EI) with estimated energy expenditure (EE). Pearson correlations identified significant variables associated with energy balance, which were then incorporated into a multiple linear regression model. The regression analysis included Profile of Mood States (POMS) anger and POMS depression as predictor variables to determine their influence on energy balance. Results: Reported mean EI was 2512 ± 1059 kcal·d−1, while estimated mean EE was 5272 ± 525 kcal·d−1. The mean energy imbalance was −2854 kcal/d (95% CI: −2655 to −3055, p < 0.001), with all participants in negative energy balance (range: −492 to −3813 kcal/d). POMS depression (r = 0.517, p < 0.01) and POMS anger (r = 0.363, p = 0.020) were associated with energy balance. The regression model was significant (R2 = 0.23, F (2, 38) = 7.02, p < 0.01), with POMS depression significantly predicting energy balance (β = 50.76, p = 0.011). Conclusions: Deployed SOF Soldiers reported high EE and limited EI, which may negatively impact performance. Higher POMS depression scores were associated with lower energy deficits. Future studies should investigate the relationship between mood and energy balance, using direct measures of EI and EE. Full article
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12 pages, 6890 KB  
Article
Engineering of Substrate-Binding Domain to Improve Catalytic Activity of Chondroitin B Lyase with Semi-Rational Design
by Miao Tian, Yuan-Yuan Xu, Yang-Nan Li, Shen Yu, Yi-Lin Wang, Xiao-Lai Ma and Ye-Wang Zhang
Curr. Issues Mol. Biol. 2024, 46(9), 9916-9927; https://doi.org/10.3390/cimb46090591 - 6 Sep 2024
Cited by 4 | Viewed by 1371
Abstract
Dermatan sulfate and chondroitin sulfate are dietary supplements that can be utilized as prophylactics against thrombus formation. Low-molecular-weight dermatan sulfate (LMWDS) is particularly advantageous due to its high absorbability. The enzymatic synthesis of low-molecular-weight dermatan sulfates (LMWDSs) using chondroitin B lyase is a [...] Read more.
Dermatan sulfate and chondroitin sulfate are dietary supplements that can be utilized as prophylactics against thrombus formation. Low-molecular-weight dermatan sulfate (LMWDS) is particularly advantageous due to its high absorbability. The enzymatic synthesis of low-molecular-weight dermatan sulfates (LMWDSs) using chondroitin B lyase is a sustainable and environmentally friendly approach to manufacturing. However, the industrial application of chondroitin B lyases is severely hampered by their low catalytic activity. To improve the activity, a semi-rational design strategy of engineering the substrate-binding domain of chondroitin B lyase was performed based on the structure. The binding domain was subjected to screening of critical residues for modification using multiple sequence alignments and molecular docking. A total of thirteen single-point mutants were constructed and analyzed to assess their catalytic characteristics. Out of these, S90T, N103C, H134Y, and R159K exhibited noteworthy enhancements in activity. This study also examined combinatorial mutagenesis and found that the mutant H134Y/R159K exhibited a substantially enhanced catalytic activity of 1266.74 U/mg, which was 3.21-fold that of the wild-type one. Molecular docking revealed that the enhanced activity of the mutant could be attributed to the formation of new hydrogen bonds and hydrophobic interactions with the substrate as well as neighbor residues. The highly active mutant would benefit the utilization of chondroitin B lyase in pharmaceuticals and functional foods. Full article
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13 pages, 1882 KB  
Article
Association of Circulating Markers of Microbial Translocation and Hepatic Inflammation with Liver Injury in Patients with Type 2 Diabetes
by Leila Gobejishvili, Vatsalya Vatsalya, Diana V. Avila, Yana B. Feygin, Craig J. McClain, Sriprakash Mokshagundam and Shirish Barve
Biomedicines 2024, 12(6), 1227; https://doi.org/10.3390/biomedicines12061227 - 31 May 2024
Cited by 2 | Viewed by 1812
Abstract
Background: Virtually the entire spectrum of liver disease is observed in association with type 2 diabetes mellitus (T2DM); indeed, T2DM is now the most common cause of liver disease in the U.S. We conducted a pilot study to investigate the relevance of increased [...] Read more.
Background: Virtually the entire spectrum of liver disease is observed in association with type 2 diabetes mellitus (T2DM); indeed, T2DM is now the most common cause of liver disease in the U.S. We conducted a pilot study to investigate the relevance of increased microbial translocation and systemic inflammation in the development of liver injury in patients with T2DM. Methods: Patients with T2DM (n = 17) and non-diabetic controls (NDC; n = 11) aged 25–80 yrs. participated in this study. Serum levels of endotoxin, calprotectin, soluble CD14 and CD163, and several inflammatory cytokines were measured. In addition to standard liver injury markers, ALT and AST, novel serum markers of liver injury, keratin 18 (K-18) M30 (apoptosis-associated caspase-cleaved keratin 18), and M65 (soluble keratin 18) were evaluated. Statistical analyses were performed using the Mann–Whitney test to assess differences between study groups. Pearson’s correlation analysis was performed to determine the strength of association between two variables using GraphPad Prism 9.5.0 software. Results: Patients with T2DM had significantly higher levels of sCD14 in comparison to NDC, suggesting an increase in gut permeability, microbial translocation, and monocyte/macrophage activation. Importantly, relevant to the ensuing inflammatory responses, the increase in sCD14 in patients with T2DM was accompanied by a significant increase in sCD163, a marker of hepatic Kupffer cell activation and inflammation. Further, a positive correlation was observed between sCD163 and endotoxin and sCD14 in T2DM patients but not in NDC. In association with these changes, keratin 18 (K-18)-based serum markers (M65 and M30) that reflect hepatocyte death were significantly higher in the T2DM group indicating ongoing liver injury. Notably, both M65 and M30 levels correlated with sCD14 and sCD163, suggesting that immune cell activation and hepatic inflammation may be linked to the development of liver injury in T2DM. Conclusions: These findings suggest that the pathogenic changes in the gut–liver axis, marked by increased microbial translocation, may be a major component in the etiology of hepatocyte inflammation and injury in patients with T2DM. However, larger longitudinal studies, including histological evidence, are needed to confirm these observations. Full article
(This article belongs to the Special Issue Advanced Research in Metabolic Syndrome)
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