Search Results (766)

Delayed or non-healing of bone defects in an aging, multi-morbid population is still a medical challenge. Current replacement materials, like autografts, are limited. Thus, artificial substitutes from biodegradable polymers and bioactive glasses (BGs) are promising alternatives. Here, novel cerium-doped mesoporous BG microparticles (Ce-MBGs) with different cerium content were included in photocrosslinkable, methacrylated gelatin (GelMA) for promoting cellular functions of human mesenchymal stem cells (hBMSCs). The composites were studied for intrinsic morphology and Ce-MBGs distribution by scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDX). They were gravimetrically analyzed for swelling and stability, compressive modulus via Microsquisher^® and bioactivity by Fluitest^® calcium assay and inductively coupled plasma-optical emission spectrometry (ICP-OES), also determining silicon and cerium ion release. Finally, seeding, proliferation, and differentiation of hBMSCs was investigated. Ce-MBGs were evenly distributed within composites. The latter displayed a concentration-dependent but cerium-independent decrease in swelling, while mechanical properties were comparable. A MBG type-dependent bioactivity was shown, while an enhanced osteogenic differentiation of hBMSCs was achieved for Ce-MBG-composites and related to different ion release profiles. These findings show their strong potential in promoting bone regeneration. Still, future work is required, e.g., analyzing the expression of osteogenic genes, providing further evidence for the composites’ osteogenic effect. Full article

As the global elderly population is rising, concerns about cognitive decline and memory loss are becoming urgent. This study evaluated the potential of sea cucumber hydrolysates (SCH) from Stichopus japonicus in alleviating cognitive deficits using a D-galactose-induced murine aging model. The effects of SCH on behavior, hippocampal morphology, gut microbiota, hippocampal cholinergic system, brain-derived neurotrophic factor (BDNF) signaling, and neuroinflammatory pathways were investigated. Results showed that SCH ameliorated learning and memory deficits and reduced neuronal damage in aging mice. SCH also modulated gut microbiota, along with increased fecal short-chain fatty acids levels. Functional prediction revealed that alterations in gut microbiota were related to signal transduction. Further, SCH enhanced hippocampal cholinergic function through elevating acetylcholine (ACh) levels and inhibiting acetylcholinesterase (AChE) activity and activated BDNF signaling, consistent with predictions of gut microbiota function. Restoration of cholinergic homeostasis and transmission of the BDNF pathway might contribute to the inhibition of hippocampal neuroinflammation via suppressing microglial activation and the nuclear factor kappa-B (NF-κB) pathway. In summary, SCH attenuated cognitive deficits through suppressing neuroinflammation, which might be correlated with the signal transduction caused by regulating gut microbiota. Further validation will be conducted through microbiota depletion and fecal microbiota transplantation. These findings suggest that SCH is a promising functional component for counteracting aging-related cognitive deficits. Full article

Purpose: The purpose of this scoping review was to systematically identify and summarize the existing literature on non-spinal clinical applications of EOS imaging and identify related evidence gaps. Method: The study followed the PRISMA-ScR guidelines. A systematic literature search was conducted in Embase, MEDLINE, CINAHL, Scopus, Cochrane, Academic Search Premier, and OpenGrey databases in November 2022 and updated in December 2023. Original research from 2003 to 2023 was eligible if in English, Danish, French, German, Norwegian, or Swedish. Two authors screened articles by title and abstract, while data extraction from full texts was performed by seven authors using a structured template. Results: A total of 8176 articles were identified, with 1350 selected for full-text review and 268 included in data extraction. Among adults, 187 articles were included, with 88 focused on surgical applications like hip arthroplasty or osteotomy. In pediatrics, 68 general and 13 surgery-related articles were included. Lower extremity analysis was the most frequent topic, with other uses identified, such as rib cage geometry, patellar dislocation, and X-linked hypophosphatemia. Conclusions: Key clinical applications of EOS imaging include lower extremity analysis, e.g., leg length assessment and knee/hip arthroplasty planning), pelvic and spinal alignment studies, and emerging uses in rib cage geometry. Evidence gaps include limited research on the diagnostic accuracy of EOS for cerebral shunt placement, reliability in bone age estimation, and an unclear role in foot and ankle morphology. Full article

This study aimed to investigate the effects of the net energy (NE) and lysine ratio in low-protein diets on growth performance, intestinal development, and cecal microbiota of male and female broilers. A 4 × 2 factorial design was used with lysine levels at 1% and 1.5%, and net energy levels at 8.93 MJ/kg and 9.76 MJ/kg were used to form four diets with net energy/lysine ratios: Group I (8.93), II (5.95), III (9.76), and IV (6.50), respectively. A total of 960 AA broilers at age of 1 d were selected; then, 480 male and 480 female broilers were randomly divided into four groups, with eight replicates per group and 15 birds per replicate. The trial lasted for 17 days, with slaughter tests conducted separately at d 7 and 17 to measure growth performance and slaughter performance. The results are as follows: (1) At d 17, broilers in high NE/lysine groups had significantly higher final weights and average daily gain compared to other groups (p < 0.01), with males weighing more than females. (2) High NE/lysine ratios (8.93 and 9.76) significantly increased the relative lengths of the jejunum and ileum from d 1 to 17 (p < 0.05). At d 7, female broilers had greater relative lengths of the duodenum, jejunum, and ileum compared to males (p < 0.05, p < 0.05, and p < 0.01), while at d 17, male broilers had greater relative lengths of the duodenum and ileum than females (p < 0.01 and p < 0.05). (3) At d 7 and 17, the villus height to crypt depth ratio in male broilers was significantly lower than that in females (p < 0.05). There was an interaction effect between NE/lysine ratios and sex on intestinal morphology. (4) High NE/lysine ratios (8.93 and 9.76) resulted in higher levels of Firmicutes and Bacteroidetes. Male broilers had higher levels of Firmicutes and Verrucomicrobia compared to females. Therefore, when lysine was at an appropriate level, a high NE/lysine ratio was more conducive to the growth and development of broilers through improving intestinal development and microbiota abundance. Female broilers showed faster intestinal development at the early age but weaker absorption capacity, while males showed dominance in intestinal length development. There were differences in characteristic gut microbiota between male and female broilers, with males having a higher abundance of energy metabolism-related microbiota. Full article

The impact of prematurity has been reported to affect ocular development during infancy and childhood. Research into long-term ocular outcomes in adults born preterm is highly relevant due to a possible impact on the development of age-related ocular diseases such as macular degeneration. The aim was to review the currently available literature regarding outcomes of prematurity on ocular morphology in adults to provide a summary of the long-term effects of prematurity and associated factors such as low birth weight (BW) and retinopathy of prematurity (ROP) and its treatment. Adults formerly born preterm have a higher prevalence of refractive error, lower visual acuity, a higher prevalence of strabismus, shorter axial length, a steeper corneal radius, increased macular thickness, and a thinner peripapillary retinal nerve fiber layer thickness (RNFL), as well as changes in vessel anatomy and the foveal avascular zone. Adults who suffered from ROP have a high risk of myopic refractive error, amblyopia, shallower anterior chambers and thicker crystalline lenses, higher corneal aberrations, thinner RNFL thickness, and foveal hypoplasia. Individuals with advanced ROP requiring treatment also have higher rates of astigmatism, an increased temporal RNFL thickness, altered macular curvature, and reduced visual acuity. Prematurity leads to lifelong ocular morphological and functional changes, suggesting that fetal origins may contribute to age-related ocular diseases. This could have implications for ophthalmologic monitoring and the frequency of check-ups in adulthood. Full article

Introduction: The rising global consumption of sugar-sweetened beverages (SSBs) has paralleled a concerning decline in sperm quality, raising concern about potential dietary impacts on male fertility. Sperm health parameters, including count, motility, and morphology, are critical indicators of reproductive potential and may be adversely affected by excessive sugar intake. This narrative review consolidates the current evidence on the association between SSB consumption and sperm health, highlighting potential biological mechanisms. Methods: A targeted literature search across PubMed, Scopus, and Google Scholar was conducted, utilising keywords “sugar-sweetened beverages”, “sperm health”, and related terms. A total of 11 eligible observational and cohort studies were selected. Studies focusing solely on animal models or unrelated dietary factors were excluded. Results: The primary research consistently reports a negative association between high SSB consumption and sperm parameters, including reduced count and motility, and increased DNA fragmentation. Potential mechanisms include oxidative stress, hormonal dysregulation, and metabolic dysfunction linked to obesity and insulin resistance. However, variability in study design, exposure assessment, and population demographics limits generalisability of the results. Conclusions: The current evidence suggests that regular SSB consumption adversely affects male reproductive health through oxidative damage and hormonal imbalances. These findings underscore the importance of public health strategies to reduce SSB intake, especially among young men of reproductive age. Further longitudinal studies with standardised methodologies, particularly in underrepresented populations such as Asian cohorts, are necessary to establish causal relationships and guide clinical recommendations. Full article

Background and Objectives: Post-term pregnancies are associated with increased risks of perinatal complications. This study aimed to evaluate potential cardiac electrophysiological changes in pregnant women by comparing the QRS duration, interval of corrected QT (QTc), and frontal QRS-T angle [f(QRS-T)] between term and post-term pregnancies. Materials and Methods: In this observational prospective study, 120 pregnant women were enrolled—60 term (37–41 weeks) and 60 post-term (≥42 weeks). All participants underwent standard 12-lead electrocardiography (ECG) and caesarean section with spinal anaesthesia. The QTc interval, QRS duration, and frontal QRS-T angle were measured. Demographic parameters such as age, gestational week, height, and weight were recorded. The SPSS software was used to analyse the data with p < 0.05 as the threshold for significance. Results: Post-operative QTc interval (417.3 ± 20.5 vs. 410.2 ± 14.5, p = 0.032) and f(QRS-T) (28 [16–55] vs. 22 [14–34], p = 0.042) were significantly higher in the post-term group than in the term group. When the change in the f(QRS-T) angle was analysed, there was a significant widening of this angle in the post-term group (from 21 [11–37] to 28 [16–55], p = 0.002). The increased f(QRS-T) angle reflects greater heterogeneity in ventricular depolarisation and repolarisation, which may indicate sub-clinical myocardial stress or altered autonomic regulation in the post-term period. Although no overt arrhythmias were observed, subtle changes in P-wave morphology and QT dispersion were more prevalent in the post-term group. Conclusions: Prolonged QRS duration and an increased f(QRS-T) angle in post-term pregnancies can reflect the underlying changes in cardiac electrophysiology related to prolonged gestation. These ECG parameters may serve as non-invasive indicators of sub-clinical cardiac stress, which could be relevant for anaesthetic risk assessment and perinatal management. Full article

Background/Objectives: Over the years, the potential of the first-trimester (FT) ultrasound in the detection of fetal structural defects has increased. The main objectives of the first-trimester fetal screening evaluation are the detection of major structural anomalies and the diagnosis of additional sonographic markers for chromosomal disorders. When a fetal anomaly is diagnosed, patients have the right to be informed about the risks, necessary interventions, or alternatives. Depending on the severity of the anomalies and the pregnancy period, the legality of the pregnancy termination was evaluated. The aim of this study was to assess the impact of the first-trimester morphological screening of the fetus using an ultrasound protocol according to the latest international protocols (the ISUOG protocol). Methods: Between 1 January 2024 and 31 December 2024, 854 pregnancies with gestational ages between 11 weeks and 13 weeks + 6 days were morphologically evaluated during the nuchal scan in the Obstetrics and Gynecology Department of the Emergency County Hospital from Craiova. Both transabdominal and transvaginal ultrasound in 2D and in a color Doppler mode were used in the scanning technique. The ultrasound findings were correlated with the genetic testing results and pregnancy outcome. The medical law implications were related to the cases where the ultrasound was performed at about 13 weeks of gestation, and the screening genetic results showed an increased pregnancy risk, which arose during the FT. In these cases, we performed amniocentesis at about 16–17 weeks of gestation, and especially, the Non-Invasive Prenatal Testing (NIPT)-positive cases were confirmed by karyotyping. Still, at this gestational age of diagnosis, the Romanian law would not allow abortions. Results: By using this extended FT ultrasound protocol, we detected 58 cases with fetal structural anomalies. Eighteen cases were also associated with genetic syndromes after performing chorionic villous sampling (CVS). Three cases detected with minor structural anomalies (two cases with club foot and one case with a cleft upper lip) were lost to follow-up. Conclusions: Fetal morphological ultrasound evaluation is feasible in the late first trimester. By using an extended ultrasound protocol, we can detect most of the fetal structural anomalies and contribute to better medical counseling and improve pregnancy outcomes. Full article

Introduction: Paediatric hip disorders present unique challenges for artificial intelligence (AI)-aided assessments of medical imaging due to disease-related and age-dependent changes in hip morphology. This scoping review aimed to describe current and emerging applications of AI in medical imaging for paediatric hip disorders. Methods: A descriptive synthesis of articles identified through PubMed, Embase, Cochrane Library, Web of Science, Emcare, and Academic Search Premier databases was performed including articles published up until June 2024. Original research articles’ titles and abstracts were screened, followed by full-text screening. Two reviewers independently conducted article screening and data extraction (i.e., data on the article and the model and its performance). Results: Out of 871 unique articles, 40 were included. The first article was dated from 2017, with annual publication rates increasing thereafter. Research contributions were primarily from China (17 [43%]) and Canada (10 [25%]). Articles mainly focused on developing novel AI models (19 [47.5%]), applied to ultrasound images or radiographs of developmental dysplasia of the hip (DDH; 37 [93%]). The three remaining articles addressed Legg–Calvé–Perthes disease, neuromuscular hip dysplasia in cerebral palsy, or hip arthritis/osteomyelitis. External validation was performed in eight articles (20%). Models were mainly applied to the diagnosis/grading of the disorder (22 [55%]), or on screening/detection (17 [42.5%]). AI models were 17 to 124 times faster (median 30) in performing a specific task than experienced human assessors, with an accuracy of 86–100%. Conclusions: Research interest in AI applied to medical imaging of paediatric hip disorders has expanded significantly since 2017, though the scope remains restricted to developing novel models for DDH imaging. Future studies should focus on (1) the external validation of existing models, (2) implementation into clinical practice, addressing the current lack of implementation efforts, and (3) paediatric hip disorders other than DDH. Full article

GH4099 is a nickel-based, high-temperature, precipitation-strengthened alloy with excellent mechanical properties and corrosion resistance, widely used in aerospace components. The performance of parts produced by additive manufacturing depends significantly on alloy powder quality and heat treatment. In this study, GH4099 alloy powder was prepared using the EIGA method, and its morphology, particle size distribution, and flowability were analyzed. The mechanical properties and microstructure of parts before and after solution-aging treatment were compared. Results showed that the powder had good sphericity and flowability, with a median diameter D50 of 28.88 μm. The formed parts underwent solution treatment at 1140 °C for 2 h followed by aging at 850 °C for 8 h. After heat treatment, the transverse tensile strength increased to 1122.11 MPa (+15.1%) and the yield strength to 866.56 MPa (+22.3%), while the longitudinal tensile strength reached 1116.81 MPa (+29.4%) and the yield strength 831.61 MPa (+35.2%). This improvement is attributed to the precipitation of γ′ phase. Fractographic analysis revealed a mixed fracture mode characterized by ductile dimples and cleavage facets, indicating that the alloy exhibits favorable toughness-related features under mechanical loading. These findings demonstrate the excellent microstructure and mechanical performance of GH4099 alloy in AM applications, providing a basis for its further use in high-performance aerospace components. Full article

Background/Objective: Aging is a significant risk factor for chronic diseases and disability, yet effective anti-aging interventions remain elusive. We explored the potential of 3-hydroxybutyrate (3HB), an endogenous metabolite with established safety, to modulate longevity in mice. Methods: In this study, we employed 2BS and WI-38 cell models, a yeast model, and naturally aging mouse models to investigate the effects of 3HB on aging in various systems. Additionally, we utilized RNA sequencing and metabolomics technologies to explore the potential mechanisms underlying the action of 3HB. Results: Our findings demonstrate that 3HB supplementation effectively delays cellular senescence, extending yeast lifespan by 51.3% and the median lifespan of naturally senescent mice by 21.0%. Notably, 3HB prolonged healthy lifespan in mice while mitigating age-related tissue morphology changes and organ senescence. Mechanistically, we identified that 3HB’s anti-aging properties are mediated through its ability to delay cellular senescence and metabolic reprogramming, while promoting the production of beneficial metabolites like trigoneline and isoguvacine. Conclusions: These findings highlight the promising therapeutic potential of 3HB as an anti-aging intervention and provide novel insights into its underlying mechanisms. Full article

Objectives: To evaluate the efficacy of faricimab in patients with neovascular age-related macular degeneration (nAMD) that did not respond to other VEGF inhibitors. Methods: This retrospective study included the eyes of patients diagnosed with nAMD who had been switched to faricimab treatment due to the persistence of intraretinal fluid (IRF) and/or subretinal fluid (SRF), despite monthly anti-VEGF treatment with aflibercept, bevacizumab, or ranibizumab using the treat and extend regimen, and who had received at least three faricimab injections following the switch. Best-corrected visual acuity (BCVA) measurement and optical coherence tomography (OCT) analysis were performed at each visit, and the OCT results were graded by two independent readers. Results: We included 41 eyes of 39 patients (21 male, 18 female) with a mean age of 80.5 ± 8.1 years. The median duration of anti-VEGF treatment prior to the switch to faricimab was 5.0 years, with a median of 53 injections. Complete resolution of IRF and SRF was observed after the first dose of faricimab in 12 eyes (29.3%) and after the third dose in 15 eyes (36.6%). Twenty-eight eyes reached a follow-up time after a switch of at least 12 months, with a median of 10 faricimab injections. Of these 28 eyes, 10 eyes (35.7%) exhibited complete IRF/SRF resolution; treatment intervals were extended beyond 4 weeks in 21 eyes (80.7%), and 8 eyes (28.6%) presented complete IRF/SRF resolution under extended treatment intervals at month 12. Central retinal thickness after 12 months was reduced from a median of 368.0 µm to 297.5 µm (p < 0.001), and the BCVA remained stable (p = 0.057). No adverse events were reported throughout the entire treatment period. Conclusions: In nAMD patients with poor anti-VEGF treatment response, complete and fast fluid resolution and the extension of treatment intervals can be reached by switching to faricimab, even after years of prior unsuccessful therapy. Full article

Introduction: Osteoporosis results from the dysregulation of osteoclast activation mechanisms. The subsequent inflammation in osteoporotic environments further hampers bone healing and impedes osseointegration. Therefore, developing treatments that can modulate osteoclast activity and regulate immune responses is essential for effectively treating osteoporotic bone defects. Methods: In this study, silver nanoparticle-decorated TiO₂ nanotubes (Ag@TiO₂-NTs) were synthesized through an electrochemical anodization technique for surface modification. The morphology and elemental composition of the Ag@TiO₂-NTs structures were characterized using scanning electron microscopy (SEM) and related methods. Subsequently, a series of in vitro and in vivo experiments were conducted to investigate the regenerative potential of Ag@TiO₂-NTs in osteoporotic bone defects. In vitro assays focused on evaluating cell viability and osteoclast function, while in vivo assessments employed osteoporotic rat models to monitor bone healing via histological examination and micro-computed tomography (micro-CT) imaging. Results: Our results demonstrated that Ag@TiO₂, through the controlled release of trace amounts of silver ions, significantly suppressed osteoclast activity and consequently alleviated bone resorption under osteoporotic conditions. In addition, Ag@TiO₂-NTs facilitated the polarization of macrophages toward the M2 phenotype. These biological effects were associated with the stimulation of autophagy, a fundamental mechanism involved in cellular repair. Moreover, the activation of autophagy contributed to the suppression of RANKL-induced NF-κB signaling, a pathway essential for the regulation of bone metabolism Conclusion: These results suggest that this surface modification strategy has the potential to be an ideal implant biomaterial for treating osteoporotic bone defects and a promising strategy for future implant surgeries. Full article

Astragalus and ginseng, esteemed as traditional Chinese herbal medicines, have demonstrated the ability to bolster physical health and enhance the immune function of organisms. In this study, the effects of a dietary astragalus–ginseng mixture on the growth performance, intestinal health, and nonspecific immunity of yellow catfish (Pelteobagrus fulvidraco) were evaluated, by measuring growth performance indices, intestinal villus morphology, enzyme activities, and expression levels of immune-related genes. Yellow catfish (n = 120, initial weight: 5.07 ± 0.18 g) were randomly assigned to four dietary groups: a control group (CT, 0 mg/kg) and three astragalus–ginseng treatment groups (AG1, 500 mg/kg; AG2, 1000 mg/kg; AG3, 2000 mg/kg). Each group had three replicates and was fed for six weeks. The results demonstrate that the treatment significantly enhanced the growth performance, as evidenced by increases in FBW, WG, WGR, SGR, and HSI. These improvements may be related to an increase in intestinal villi length and increased LPS activity, both of which are associated with enhanced digestive function. Meanwhile, the activity of antioxidant enzymes in the liver, including CAT, SOD, and GSH, was increased, whereas the level of MDA was decreased. In the serum, GSH was up-regulated, while SOD activity was decreased. Immune-related enzyme activities, such as ALT and LZM, were up-regulated, while AST showed no significant difference. Moreover, the treatment also promoted the expression of the anti-inflammatory factor IL-10. The pro-inflammatory factors, such as IL-1β, IL-6, and TNF-α, were decreased with the addition of low concentrations but increased with high concentrations. In conclusion, supplementation with an astragalus–ginseng mixture could promote growth performance by increasing digestive enzyme activity and intestinal villi length, and improve disease and stress resistance traits by modulating immune genes and antioxidant enzyme activity. A dosage of 1000 mg/kg was found to be optimal. Full article

Atrial fibrillation (AF) is the most frequently observed type of arrhythmia among adults, and its absolute prevalence is steadily rising in close association with the aging of the population, with its prevalence varying from 2% in the general population to 10–12% among the elderly. The relatively new concepts of ‘atrial cardiomyopathy’ and “AF-related atrial cardiomyopathy”, along with the growing body of knowledge regarding remodeling, function, and tissue characterization, highlight the need for novel approaches to the diagnostic process as well as in the therapeutic guidance and monitoring of atrial arrhythmias. Advanced imaging techniques, particularly cardiac magnetic resonance (CMR) imaging, have emerged as pivotal in the detailed assessment of atrial structure and function. CMR facilitates the precise measurement of left atrial volume and morphology, which are critical predictors of AF recurrence post-intervention. Furthermore, it enables the evaluation of atrial fibrosis using late gadolinium enhancement (LGE), offering a non-invasive method to assess the severity and distribution of fibrotic tissue. The possibility of an accurate CMR pulmonary vein anatomy mapping enhances the precision of pulmonary vein isolation procedures, potentially improving outcomes in AF management. This review underlines the integration of novel diagnostic tools in enhancing the understanding and management of AF, advocating for a shift towards more personalized and effective therapeutic programs. Full article