Search Results (15,126)

In this study, a new approach was developed for the estimation of optimum parameters (ODP), in terms of materials and design in livestock barns, and for optimal design. For this purpose, two thousand simulations were run using Monte Carlo (MC) techniques and Latin hypercube methods using the Energy Plus program on a 50-head closed dairy farm. In this study, the heat balance in the barn was adapted to Energy Plus using an innovative approach, using heat balance equations according to the ASHRAE Standard. First, data normality was determined using the Shapiro–Wilk (SW) and Kolmogorov–Smirnov (KS) tests. Data on thermal stress duration and energy consumption for dairy cattle welfare were estimated directly from the simulations, and sensitivity (SA) and uncertainty (UA) analyses were conducted. Furthermore, the statistical relationship between thermal comfort and energy consumption was determined using Pearson correlation. The predicted values obtained from the simulations were validated with barn values, and time-series overlay plots and histograms were generated. Furthermore, interpretations of the validation processes were made based on MBE, RSME, and R² statistical values. The study estimated an indoor thermal comfort temperature of 12 °C, and this value was taken into account in the innovatively developed simulations. The estimated optimum design parameters in the study resulted in energy reductions of 25% and 41% for walls and roofs, 48% and 19% for cooling and heating setpoint temperatures, 43% and 37% for window areas, and 75% and 40% for natural and mechanical ventilation, respectively. When the design parameters were evaluated holistically and analyzed in terms of average values, the new simulation model achieved approximately 50% energy savings. We believe that the newly developed approach will guide future planning for countries, the public, and private sectors to ensure animal welfare and reduce energy consumption. Full article

Organophosphates, especially their ester, are not only toxic to humans but equally toxic to aquatic and other animal life on Earth when exposed to them. Here, we designed an efficient and easy way to degrade hexamethyl phosphoramide and omethoate organophosphate catalytically in a natural way into non-toxic products. Both hexamethyl phosphoramide and omethoate are possible carcinogens and cause serious health issues in humans and other animals when exposed to them. In this work, a modified sonochemical method was used for the synthesis of ZnO nanoparticles using zinc acetate dihydrate, ethylenediamine dihydrochloride, and polyvinylpyrrolidone. Sodium hydroxide was used as the precipitating agent, and distilled water was used as the solvent. An Elmasonic ultra-sonicator with 240-watt power was used for the preparation of ZnO nanoparticles. The synthesized ZnO nanoparticles with a high surface area (250 m²/g), average particle size of 23 ± 1 nm, and a mesoporous structure with 1.858 nm average pore size were then used for the degradation of organophosphate, i.e., hexamethyl phosphoramide and omethoate pesticide, using 10 µL of concentration to check their catalytic efficiency for the first time. The degradation products were identified using gas chromatography–electron impact mass spectrometry (GC/EIMS). The results showed that omethoate was completely degraded, while hexamethyl phosphoramide showed partial degradation, both producing fewer toxic intermediates. Full article

Early testicular development is vital for adult male fertility but remains highly vulnerable to stress during the suckling stage. Fermented Chinese chive (Allium tuberosum) is known for its antioxidant and immunomodulatory properties, yet its role in testicular development remains unclear. In this study, Songliao Black piglets received 3% fermented Chinese chive (LK group) mixed with starter feed and compared to a control (OD group). Testicular samples at weaning (28 days) underwent transcriptomic and metabolomic analyses. Although no significant differences were observed in gross testicular morphology, the LK group significantly increased individual (13.85%) and litter (15.11%) weaning weights (p < 0.05), with elevated serum triglycerides, total cholesterol, and a 32.2% rise in IgG levels (p < 0.05). Integrated analysis identified 76 shared pathways, including ferroptosis, insulin resistance, PI3K-Akt, MAPK, and cAMP signaling. Upregulated genes in the LK group were mainly related to energy metabolism, antioxidant defense, immune regulation, steroidogenesis, and neuroendocrine signaling, suggesting improved metabolic activity, reduced oxidative stress, and accelerated reproductive maturation. Molecular docking indicated that kaempferol and isorhamnetin from Chinese chive bind strongly to proteins involved in testicular development. Overall, fermented Chinese chive supplementation enhances early testicular development in suckling piglets via integrated modulation of metabolic, immune, and signaling pathways, providing a nutritional strategy to optimize reproductive potential in breeding boars. Full article

The livestock sector in Serbia has been experiencing a prolonged period of structural and economic challenges, characterized by decreasing animal numbers, low productivity, and reduced competitiveness in both domestic and EU markets. This study analyses the key structural, technological, economic, and policy factors shaping these trends to provide strategic recommendations for sustainable sector revitalization. The methodology integrates macroeconomic analysis, agricultural economic accounts, and international trade data, applying regression modelling to examine relationships between domestic food prices, exchange rates, and agri-food import volumes. The results indicate that livestock’s share of agricultural gross value added remains below 35%, significantly lower than EU averages, while export quotas remain underutilized and the trade balance for animal products is persistently negative. Contributing factors include fragmented farm structures, outdated production technologies, limited adoption of innovations, demographic decline in rural areas, and insufficient alignment with EU CAP Strategic Plans and Green Deal objectives. Climate change impacts, such as droughts and heat stress, alongside animal disease outbreaks and macroeconomic pressures, further exacerbate these vulnerabilities. The study recommends modernizing production systems through investment in technological upgrades, strengthening farmer organizations and cooperatives, enhancing biosecurity and animal welfare standards, and improving policy frameworks to align with EU sustainability objectives. Emphasis is placed on developing integrated approaches that simultaneously address productivity, economic resilience, and environmental sustainability. Implementing these strategic measures is essential for enhancing food security, supporting rural development, and ensuring Serbia’s successful integration into the EU market as part of a more sustainable and resilient agri-food system. Full article

This study investigated the effects of genotype and sex on carcass traits, myosin heavy chain (MyHC) isoforms, and meat quality in pigs raised under Thai commercial conditions. Fifty pigs (25 barrows and 25 gilts) from five genotypes—purebred Duroc (D), LWLR1 (Large White_old × Landrace_old), LWLR2 (Large White_new × Landrace_new), DLWLR1 (Duroc × LWLR1), and DLWLR2 (Duroc × LWLR2)—were slaughtered at ~110 kg body weight. LWLR1 is traditional maternal line, whereas LWLR2 is a newly selected line for higher intramuscular fat (IMF). Significant genotype × sex interactions were observed for dressing percentage, carcass length, bone percentage, purge loss, pH₂₄, and L* (lightness) (p < 0.05). The Longissimus lumborum (LL) muscle of Duroc pigs showed the lowest pH₄₅ and shear force but highest IMF, whereas LWLR1 pigs exhibited higher crude protein, polyunsaturated fatty acids, and flavor-enhancing nucleotides. The LL muscle of LWLR2 and DLWLR2 pigs showed greater expression of MyHC I and IIA, as well as calpain 1 (CAPN1), calpain 2 (CAPN2), and calpastatin (CAST) genes. In contrast, Duroc pigs had the highest MyHC IIX and lowest IIB expression, opposite to patterns in LWLR1 and DLWLR1. Principal component analysis revealed distinct genotype-based clustering, emphasizing the influence of maternal lineage on pork quality and offering valuable insights for genetic selection strategies. Full article

Introduction: Plant-based phospholipid (PP) liposomes are sustainable, biocompatible, and biodegradable carriers with advantages over synthetic and animal-derived lipids, including lower immunogenic risk and abundant availability from sources such as soy, sunflower, and canola. This systematic review examines their characteristics, innovations, and applications in breast cancer (BCA) therapy. Methods: A total of 43 studies published between 2010 and June 2025 were identified from MEDLINE, Scopus, and Web of Science, focusing on PP composition, drug delivery mechanisms, and therapeutic efficacy in in vitro, in vivo, and preclinical BCA models. Results: Advances include nanotechnology and ligand-targeted systems that improve stability, control drug release, and enhance tumor-specific uptake. PP liposomes co-loaded with chemotherapeutics showed synergistic anticancer effects, increased tumor accumulation, and reduced systemic toxicity. Personalized targeting strategies further improved therapeutic precision and minimized off-target effects. Conclusions: PP liposomes offer an innovative and environmentally sustainable approach for BCA treatment with demonstrated preclinical benefits in efficacy and safety. Translation to clinical practice requires standardized characterization, scalable production, and well-designed trials to confirm safety, dosing, and long-term effectiveness. Full article

Aquaculture is the fastest-growing food production sector and plays a pivotal role in global food security. However, the reliance on conventional fishmeal and fish oil in aquafeeds raises sustainability concerns due to overfishing, high costs, and ecological burden. This review explores the valorisation of microalgae as a sustainable and functional alternative for aquafeed development. Microalgae are rich in proteins, polyunsaturated fatty acids, bioactive compounds, and pigments that support aquatic animal growth, immunity, and product quality. We critically examine the integration of green technologies, including cultivation systems, biomass harvesting, and eco-friendly extraction methods for optimising microalgal biomass and bioactive recovery. The review also discusses recent innovations in bioremediation and circular aquaculture systems, highlighting the role of microalgae in reducing nutrient discharge, carbon footprint, and operational cost. Challenges such as scalability, digestibility, and economic feasibility are also addressed, providing insight into pathways toward industrial adoption. This review aims to provide an updated and holistic perspective on microalgae-based aquafeeds in advancing sustainable aquaculture practices. Full article

In recent years, scientific interest in functional ingredients capable of replacing the non-therapeutic use of antibiotics in animal feed has intensified, fostering the exploration of novel additives such as Saccharomyces cerevisiae hydrolysate (SCH). This study investigated the effect of dietary SCH supplementation on growth performance, intestinal morphology, local immune response, and cecal microbiota composition in Ross 308 broiler chickens. A total of 300 one-day-old male chicks were randomly assigned to two experimental groups, receiving either a standard diet or a diet supplemented with SCH (500 mg/kg during the starter and grower phases; 250 mg/kg during the finisher phase). SCH supplementation significantly improved growth performance during the finisher phase, with increases in final body weight (p = 0.025), average daily gain (p = 0.049), and average daily feed intake (p = 0.027), without significant changes in feed conversion ratio (p > 0.05). Favourable intestinal morphological modifications were observed, with a significant increase in villus height to crypt depth ratio in both the jejunum and ileum at days 28 (p = 0.035 and 0.002, respectively) and 42 (p < 0.001). The expression of pro-inflammatory cytokine genes was significantly reduced, with lower levels of TNF-α, IL-1β, and IL-6, while tight junction protein genes ZO-1 and Occludin were significantly upregulated (p < 0.05). Microbiota profiling revealed higher alpha diversity and greater abundance of Prevotella. These findings highlight SCH as a promising dietary strategy to improve broiler performance, intestinal function, and sustainability in poultry production systems. Full article

Antimicrobial resistance (AMR) constitutes a serious public health issue, and the European Union (EU) requires reduction in the sales of antibiotics in farmed animals of 50% by 2030. A scoping review was conducted in PubMed for the years from 2000 to 2024, limited to the English and German languages, with the aims to (1) provide an overview of factors on commercial poultry farms potentially associated with health-related endpoints such as mortality, disease prevalence, carcass condemnation, performance as well as AMR/antimicrobial usage at different hierarchical levels (animal, flock/batch, stable, farm), and (2) identify inconsistencies with respect to these potential risk factors. Overall, 34 peer-reviewed publications met the inclusion criteria for the review. Significant associations identified in the uni- or multivariable statistical analysis were summarised using graphs and bar charts. The results highlight that risk factor–outcome associations often are complex, inconsistent with regards to the direction of the influence especially for some ordinal or categorical variables. In some associations such as the sex of the animals and performance, contrary directions were reported in different studies—illustrating the multifactorial dynamics of commercial poultry production. This research enhances the understanding of the complexity of commercial poultry production, which is essential when designing future studies and interpreting their results. Full article

Zearalenone (ZEN), a mycotoxin produced by Fusarium species, widely contaminates grains and feed, posing a serious threat to animal and human health. Traditional physical and chemical detoxification methods face challenges, including low efficiency, high costs, and nutrient loss. In contrast, enzymatic biodegradation has emerged as a research hotspot due to its high efficiency, specificity, and environmental friendliness. Lactone hydrolase can specifically hydrolyze the lactone ring of ZEN, converting it into a low-toxicity or non-toxic degradation product, thereby demonstrating significant potential for application in ensuring the safety of food, feed, and agricultural products. In recent years, with advancements in enzyme engineering and various biological technologies, remarkable progress has been made in ZEN-degrading enzyme research. Novel and highly efficient enzyme genes have been discovered through gene mining, while directed evolution and rational design have improved catalytic efficiency and stability. Additionally, immobilization techniques and formulation optimization have enhanced industrial applicability. This review, based on practical application needs, establishes a comprehensive evaluation system integrating enzyme characteristics, modification technologies, and process applicability, aiming to provide actionable theoretical guidance for the large-scale application of biological detoxification technologies. Full article

Increasingly complex epidemics of animal infectious diseases have emerged as a major risk to livestock production and human health. However, current detection methods for animal infectious diseases suffer from shortcomings such as insufficient sensitivity, complicated operation, and reliance on skilled personnel, highlighting the urgent need for novel sensing platforms. CRISPR/Cas systems are adaptive immune systems found in many prokaryotes. Owing to their ability to precisely and reliably target and cleave nucleic acids, the CRISPR/Cas-based nucleic acid detection technology is considered a promising new detection method. When leveraged with a pre-amplification step and established readout methods, CRISPR/Cas-based sensing platforms can achieve a high sensitivity of single-base resolution or attomolar levels on-site. In this review, we first outline the history, working principles, and nucleic acid detection platforms derived from various CRISPR/Cas systems. Next, we evaluate the advantages and limitations of different nucleic acid pre-amplification methods integrated with CRISPR/Cas systems, followed by a discussion of readout methods employed in CRISPR/Cas-based sensing platforms. Additionally, we highlight recent applications of CRISPR/Cas-based sensing platforms in identifying animal infectious diseases. Finally, we address the challenges and prospects of CRISPR/Cas-based sensing platforms for the early and accurate diagnosis of animal infectious diseases. Full article

Hydrothermal vents are “oases” of biological productivity and endemicity on the seafloor. Chemosynthetic communities at deep-sea hydrothermal vents are characterized by high abundance and endemism. The distribution of species among these isolated habitats supports regional biodiversity and stability, so understanding the fundamental processes is a key target of conservation. Larval dispersal resulting from deep-ocean circulations is one of the major factors influencing the diversity and distributions of vent animals. By combining a biophysical model with biological larvae traits, we quantify potential larval dispersal of vent species via ocean circulation in the Azores Triple Junction. Here we present results from a biophysical model of larval dispersal run for the hydrothermal vent benthic mussel Bathymodiolus azoricus. Several scenarios were implemented, based on similar data sets, although changing values for one or two parameters, such as swimming behaviour and planktonic larvae duration. Results showed that larvae retention is the most common pattern from the Azores Triple Junction vent fields mussel. The Rainbow vent field is rather isolated, being the sink population of the Menez Gwen and Lucky Strike but with a very low number of larvae exchange. Results are discussed in the framework of spatial management to maintain the populations after an impact by natural or human disturbance. Full article

The increasing demand for poultry meat calls for sustainable production methods that address animal welfare and combat antimicrobial resistance (AMR). Commensal Escherichia coli serve as reservoirs of resistance genes that may transfer to pathogens, facilitating AMR spread in agriculture. This study evaluated the efficacy of a bacteriophage cocktail, UPWr_E, applied as a litter spray to reduce total and antibiotic-resistant E. coli in broiler chicken rearing. The cocktail, containing four lytically active phages, was administered for four weeks. Microbiological analyses of litter, feces, and cecal contents showed a significant reduction in total E. coli by 3.2 log₁₀ CFU/g in litter and a decrease in resistant strains to gentamicin, enrofloxacin, tetracycline, and sulfamethoxazole–trimethoprim, compared to controls. No significant changes occurred in E. coli loads in feces or cecal contents, indicating limited impact on the number of commensal E. coli in cecal contents. Phages remained detectable and stable in litter and feces throughout the study. These findings demonstrate the potential of phage therapy as a targeted, environmentally friendly approach to control AMR reservoirs in poultry farming. Incorporating phage-based treatments into AMR management strategies could improve food safety and promote sustainable animal production. Full article