Search Results (45)

Extending the fruiting season of Annona squamosa L. requires overcoming autumn and winter flowering declines. This study investigates the efficacy of light-quality regulation technologies and their temperature dependence for floral induction. Field surveys initially identified temperature as the primary climatic factor governing flowering. Under suboptimal autumn temperatures, red light (R-660) night-break (NB) treatments significantly enhanced shoot growth and flowering compared to other light spectra. Transcriptomic analysis revealed 2027 upregulated and 341 downregulated transcripts consistently regulated by R-660, with significant enrichment in the plant hormone signal transduction pathway. Furthermore, R-660 upregulated cold response genes (e.g., CBFs, WRKYs, ERD7), which are associated with the maintenance of vegetative vigor under suboptimal autumn temperatures. However, mid-winter R-660 NB failed to induce flowering without supplemental greenhouse heating. Ultimately, warm ambient temperature is the absolute prerequisite for A. squamosa floral induction, with R-660 serving as a highly effective seasonal supplement to extend autumn flowering. Full article

The objective of this study was to evaluate the insecticidal potential of custard apple (Annona squamosa L.) seed extract against Chrysodeixis includens, a major soybean pest, and to assess its toxicological effects on a non-target organism. The extract was obtained by ultrasound-assisted extraction using water as the solvent. Ingestion bioassays exposed C. includens larvae to aqueous extract concentrations ranging from 0.25 to 10.0 g/L, with Dipel WG^® (5 g/L) and an untreated diet used as positive and negative controls, respectively. Mortality rates ranged from 40 to 97%, increasing with exposure time and concentration up to 1 g/L and remaining statistically stable at higher concentrations after 120 h. Extract concentrations from 1 to 10 g/L did not differ significantly from the positive control. Ecotoxicological assays were conducted using zebrafish (Danio rerio) exposed to extract concentrations of 0.84–16.80 µg/L, and biomarkers of neurotoxicity and oxidative stress were evaluated. At 16.8 µg/L, the extract caused lower biomarker alterations than Dipel WG^®, indicating reduced oxidative damage. Overall, A. squamosa seed extract effectively controls C. includens at low concentrations with minimal environmental risk to non-target organisms. Full article

Root rot disease severely threatens tropical fruit production, leading to plant mortality and reduced yields; however, the mechanisms of host defense responses and pathogen infection remain poorly understood. In this study, Fusarium acutatum was isolated from diseased Annona squamosa roots and identified through morphological features and ITS phylogeny (99.8% identity). Infection triggered a marked activation of antioxidant defenses, with elevated POD, SOD, PAL, PPO, and CAT activities. Transcriptomic and TMT-based quantitative proteomic analyses identified 23,791 and 74,403 differentially expressed genes (DEGs) and 367 and 609 differentially expressed proteins (DEPs) in root at 5 and 10 days post inoculation, respectively, relative to the control. These DEGs and DEPs were consistently enriched in pathways involving redox regulation, protein synthesis and processing, ubiquitin-mediated proteolysis, phenylpropanoid and flavonoid metabolism, cell wall remodeling, plant–pathogen interaction and MAPK signaling. Integrated transcriptomic–proteomic correlation analysis showed clear positive associations between key defense-related genes and proteins, suggesting that phenylpropanoid metabolism and reactive oxygen species (ROS) scavenging play central roles in resistance. Key genes such as CHI2, CHS, and CYP were strongly induced and validated by qPCR, supporting coordinated activation of the defense systems. Furthermore, F. acutatum exhibited upregulation of 50 pathogenic-related proteins, including 4 cell wall-degrading enzymes (e.g., CBH1, pectate lyase), 5 metabolic regulation or signal transduction enzymes (e.g., gabD, TPI, and ENO) and 3 potential effectors, suggesting coordinated pathogen strategies for host colonization. Collectively, this study provides comprehensive multi-omics insight into the molecular mechanisms underlying A. squamosa defense against F. acutatum and offers candidate targets supported by omics evidence, serving as a theoretical reference for the management of root rot. Full article

The custard apple (CA) is a noble fruit in tropical regions worldwide. It has attracted a growing interest due to its organoleptic properties and nutritional value. With the expansion of international trade, both its cultivation and consumption have grown significantly in recent years. Previous researchers have sporadically investigated its nutritional composition and health benefits; however, existing information on its processing and utilization is highly fragmented and lacks a comprehensive overview of its constituents, biological activities, and potential applications. This review is a detailed summary of the nutritional and bioactive properties, safety evaluations, and potential applications of CA. Following PRISMA guidelines, peer-reviewed studies published between 2000 and 2025 were systematically searched in PubMed, Scopus, ResearchGate, and Web of Science. Inclusion criteria comprised studies reporting on nutritional composition, phytochemicals, bioactivities, health promotion, and applications of CA. In addition to primary nutrients like carbohydrates, protein, fatty acids, vitamins, and minerals, CA also contains a multitude of bioactive compounds, mainly including phenols, flavonoids, terpenoids, acetogenins, and alkaloids, which are attributed to a range of health benefits, such as antioxidant, anti-microbial, anti-tumor, blood sugar regulation, and cognitive function improvement. However, more clinical and toxicological profiles remain underexplored, and future research should focus on standardized extraction, safety evaluation, and translational applications. Additionally, the challenges and future perspectives in industrial applications are discussed, which are expected to offer comprehensive information for the utilization of CA. Full article

Sugar apple (Annona squamosa) is prized for its excellent taste, rich nutrition, and diverse uses, making it valuable for both fresh consumption and medicinal purposes. Predominantly found in tropical regions of the Americas and Asia, its harvesting remains labor-intensive in orchard settings, resulting in low efficiency and high costs. This study investigates the use of computer vision for sugar apple instance segmentation and introduces an improved deep learning model, GCE-YOLOv9-seg, specifically designed for orchard conditions. The model incorporates Gamma Correction (GC) to enhance image brightness and contrast, improving target region identification and feature extraction in orchard settings. An Efficient Multiscale Attention (EMA) mechanism was added to strengthen feature representation across scales, addressing sugar apple variability and maturity differences. Additionally, a Convolutional Block Attention Module (CBAM) refined the focus on key regions and deep semantic features. The model’s performance was evaluated on a self-constructed dataset of sugar apple instance segmentation images captured under natural orchard conditions. The experimental results demonstrate that the proposed GCE-YOLOv9-seg model achieved an F1 score (F1) of 90.0%, a precision (P) of 89.6%, a recall (R) level of 93.4%, a mAP@0.5 of 73.2%, and a mAP@[0.5:0.95] of 73.2%. Compared to the original YOLOv9-seg model, the proposed GCE-YOLOv9-seg showed improvements of 1.5% in the F1 score and 3.0% in recall for object detection, while the segmentation task exhibited increases of 0.3% in mAP@0.5 and 1.0% in mAP@[0.5:0.95]. Furthermore, when compared to the latest model YOLOv12-seg, the proposed GCE-YOLOv9-seg still outperformed with an F1 score increase of 2.8%, a precision (P) improvement of 0.4%, and a substantial recall (R) boost of 5.0%. In the segmentation task, mAP@0.5 rose by 3.8%, while mAP@[0.5:0.95] demonstrated a significant enhancement of 7.9%. This method may be directly applied to sugar apple instance segmentation, providing a promising solution for automated sugar apple detection in natural orchard environments. Full article

Due to the increasing demand for sustainable energy, non-edible oilseed crops are being explored as alternatives to traditional edible oils. Annona squamosa seeds are rich in oil content (24%/100 g) and often discarded as agricultural waste. Determination of mechanical properties of the seeds under compression loading is significant for designing machinery for its handling and processing. Thus, the present study assessed the effect of loading speeds, $LS,$ (5.0–25 mm/min) and moisture contents, $ms,$ (8.0–32.5%, db) on rupture force and energy, bioyield force and energy, deformation, and hardness at the seed’s horizontal and vertical orientations using a Testometric Universal Testing Machine. The results indicate that both $LS$ and $mc$ significantly ($p<0.05$) affect the mechanical properties of the seeds. Particularly, horizontal loading orientations consistently exhibited higher values for the selected compressive properties than vertical orientations, except for deformation at varying $LS$. The correlations between $LS$, $mc$, and the compressive parameters of the seed were mostly linear, at both orientations, with increasing $mc$ from 8.0 to 32.5% (db). High correlation coefficients ($R^2$) were obtained for the relationship between the studied parameters, $LS$, and $mc$. The data obtained would provide crucial insights into optimizing oil extraction processes by enabling the design of efficient machinery that accommodates the unique characteristics of the seeds. Thus, the findings contribute to the growing interest in alternative biodiesel feedstock, demonstrating that A. squamosa seeds can be repurposed for economic and environmental benefits. Full article

Background: Higenamine, a bioactive alkaloid derived from plants such as Aconitum and Annona squamosa, has been traditionally used in Chinese medicine for treating heart diseases like bradycardia, arrhythmia, and heart failure. It exhibits multiple pharmacological effects, including anti-oxidative stress, improved cellular energy metabolism, anti-apoptosis, and enhanced erectile dysfunction. Aim and Methods: To investigate the reasons for these functions of higenamine and its application in the clinic, the literature of the database was searched and read in this study. Results: As a non-selective β-agonist, higenamine activates both β₁- and β₂-adrenergic receptors, leading to cardiovascular benefits such as increased heart rate and myocardial contractility, as well as bronchodilation. It has also been studied for its potential in weight loss, anti-inflammatory properties, and antioxidant properties, with applications in treating asthma, cardiovascular diseases, and ischemia-reperfusion injuries. However, its clinical use is limited by the need for further research on its long-term safety, pharmacokinetics, and interactions with other drugs. Despite its promising therapeutic potential, higenamine’s inclusion in the World Anti-Doping Agency’s banned list highlights concerns over its stimulant effects and safety in athletic contexts. Conclusions: Future studies should focus on optimizing its clinical applications while ensuring safety and efficacy. In terms of clinical applications, future research will also be able to explore more possibilities to use higenamine more in the treatment of diseases. Full article

One of the most important nutrients needed for fruit tree growth is nitrogen. For orchards to get targeted, well-informed nitrogen fertilizer, accurate, large-scale, real-time monitoring, and assessment of nitrogen nutrition is essential. This study examines the Leaf Nitrogen Content (LNC) of the custard apple tree, a noteworthy fruit tree that is extensively grown in China’s Yunnan Province. This study uses an ensemble learning technique based on multiple machine learning algorithms to effectively and precisely monitor the leaf nitrogen content in the tree canopy using multispectral canopy footage of custard apple trees taken via Unmanned Aerial Vehicle (UAV) across different growth phases. First, canopy shadows and background noise from the soil are removed from the UAV imagery by using spectral shadow indices across growth phases. The noise-filtered imagery is then used to extract a number of vegetation indices (VIs) and textural features (TFs). Correlation analysis is then used to determine which features are most pertinent for LNC estimation. A two-layer ensemble model is built to quantitatively estimate leaf nitrogen using the stacking ensemble learning (Stacking) principles. Random Forest (RF), Adaptive Boosting (ADA), Gradient Boosting Decision Trees (GBDT), Linear Regression (LR), and Extremely Randomized Trees (ERT) are among the basis estimators that are integrated in the first layer. By detecting and eliminating redundancy among base estimators, the Least Absolute Shrinkage and Selection Operator regression (Lasso)model used in the second layer improves nitrogen estimation. According to the analysis results, Lasso successfully finds redundant base estimators in the suggested ensemble learning approach, which yields the maximum estimation accuracy for the nitrogen content of custard apple trees’ leaves. With a root mean square error (RMSE) of 0.059 and a mean absolute error (MAE) of 0.193, the coefficient of determination (R²) came to 0. 661. The significant potential of UAV-based ensemble learning techniques for tracking nitrogen nutrition in custard apple leaves is highlighted by this work. Additionally, the approaches investigated might offer insightful information and a point of reference for UAV remote sensing applications in nitrogen nutrition monitoring for other crops. Full article

The utilization of nano-sized drug delivery systems in herbal drug delivery systems has a promising future for improving drug effectiveness and overcoming issues connected with herbal medicine. As a consequence, the use of nanocarriers as novel drug delivery systems for the improvement of traditional medicine is critical to combating infectious diseases globally. In line with this, we herein report the biosynthesis of silver nanoparticles (AgNPs), gold nanoparticles (AuNPs), and bimetallic nanoparticles (BMNPs) as antibacterial agents against pathogenic bacterial strains using Annona squamosa L. leaf extract as a bio-reductant and bio-stabilizing agent. The as-synthesized metal nanoparticles were characterized by transmission electron microscopy (TEM), ultraviolet–visible (UV–Vis) absorption spectroscopy, X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), and the dynamic light scattering (DLS) method. The as-synthesized MNPs had an average particle size of 6.98 nm ± 2.86 nm (AgNPs), 21.84 ± 8.72 nm (AuNPs), and 2.05 nm ± 0.76 nm (BMNPs). The as-synthesized AgNPs and BMNPs showed good antibacterial activity against pathogenic bacterial strains of Gram-positive Staphylococcus aureus (ATCC 25923) and Gram-negative Escherichia coli (ATCC 25922). The obtained results offer insight into the development of benign nanoparticles as safe antibacterial agents for antibiotic therapy using Annona squamosa L. leaf extract. Full article

Accurate diagnosis of plant diseases is crucial for crop health. This study introduces the EDA–ViT model, a Vision Transformer (ViT)-based approach that integrates adaptive entropy-based data augmentation for diagnosing custard apple (Annona squamosa) diseases. Traditional models like convolutional neural network and ViT face challenges with local feature extraction and large dataset requirements. EDA–ViT overcomes these by using a multi-scale weighted feature aggregation and a feature interaction module, enhancing both local and global feature extraction. The adaptive data augmentation method refines the training process, boosting accuracy and robustness. With a dataset of 8226 images, EDA–ViT achieved a classification accuracy of 96.58%, an F1 score of 96.10%, and a Matthews Correlation Coefficient (MCC) of 92.24%, outperforming other models. The inclusion of the Deformable Multi-head Self-Attention (DMSA) mechanism further enhanced feature capture. Ablation studies revealed that the adaptive augmentation contributed to a 0.56% accuracy improvement and a 0.34% increase in MCC. In summary, EDA–ViT presents an innovative solution for custard apple disease diagnosis, with potential applications in broader agricultural disease detection, ultimately aiding precision agriculture and crop health management. Full article

Background/Objectives: Annona squamosa is used in folk medicine to treat pain and arthritis. Palmatine is an alkaloid isolated from several plants, including A. squamosa leaves. The aim of the present study was to investigate the analgesic, anti-arthritic, and anti-inflammatory potential of the methanolic extract of A. squamosa (EMAS) and palmatine. Methods: The chemical profile of EMAS was evaluated by ultra high-performance liquid chromatography with electrospray ionization coupled to mass spectrometry (UHPLC-ESI/MS). EMAS and palmatine were evaluated in carrageenan-induced pleurisy, zymosan-induced joint inflammation, formalin-induced nociception, and tumor necrosis factor (TNF)-induced mechanical hyperalgesia in experimental models in mice. A cytotoxicity test of EMAS and palmatine was performed using a methylthiazolidiphenyl-tetrazolium (MTT) bromide assay. Results: The analysis of the chemical profile of the extract showed the presence of palmatine, liriodenine, and anonaine. Oral administration of EMAS and palmatine significantly reduced leukocyte migration and oxide nitric production in the carrageenan-induced pleurisy model. EMAS and palmatine reduced mechanical hyperalgesia, leukocyte migration, and edema formation in the joint inflammation induced by zymosan. In the formalin test, palmatine was effective against the second-phase nociceptive response, mechanical hyperalgesia, and cold allodynia. In addition, palmatine reduced mechanical hyperalgesia induced by TNF. EMAS and palmatine did not demonstrate cytotoxicity. Conclusions: The present study showed that A. squamosa and palmatine are analgesic and anti-inflammatory agents, and that the anti-hyperalgesic properties of palmatine may involve the TNF pathway. Palmatine may be one of the compounds responsible for the anti-hyperalgesic and/or anti-arthritic properties of this medicinal plant. Full article

Seasonal drought and summer soil high-temperature stress in Southern China often lead to decreased yield and quality of Annona squamosa. It is important to explore reasonable and effective water and fertilizer management measures as well as cover measures to improve the soil hydrothermal conditions in orchards to realize the increase in yield and quality of Annona squamosa. This study involved a two-year (2022–2023) field experiment in Yun County, Lincang City, Yunnan Province, using three factors and a three-level orthogonal test, resulting in nine different experimental treatments for water, fertilizer and heat. The three irrigation levels were W1 (soil moisture content of 55% of field moisture capacity), W2 (soil moisture content of 75% of field moisture capacity) and W3 (soil moisture content of 85% of field moisture capacity). The three fertilizer levels were F1 (1666 kg·hm⁻²), F2 (2083 kg·hm⁻²) and F3 (2500 kg·hm⁻²), and the three cover methods were A1 (no cover), A2 (fresh grass cover) and A3 (straw cover). The effects of these treatments on soil hydrothermal conditions, growth indices and fruit yield and quality of Annona s1uamosa were systematically monitored and analyzed, and the relationships between these treatments and yield and quality was analyzed based on a Mantel test. The results showed that T5 (W2F2A3) had the highest average soil moisture content over two years, followed by T7 (W3F1A3). The T7 (W3F1A3) treatment effectively reduced soil temperature by 5 °C compared to T1 (W1F1A1). T5 (W2F2A3) had the highest average yield over two years, with an increase of 33.99% compared to T1 (W1F1A1). Additionally, T5 (W2F2A3) has the highest average soluble solids, soluble sugars and vitamin C content over two years, with increases of 28.13%, 13.36% and 4.86%, respectively, compared to T1 (W1F1A1). A Pearson correlation analysis showed that there was a significant correlation between Annona squamosa growth and soil moisture content and soil temperature, and the Mantel test showed that soil hydrothermal conditions had significant influence on the growth and yield. T5 (W2F2A3) has the highest comprehensive benefit in promoting growth, increasing yield and improving quality for the plant. The effects of different irrigation quantities, fertilizer amounts and different cover measures on the coupling interaction for soil hydrothermal status in the root zone, growth, yield and quality of Annona squamosa were investigated, providing reliable theoretical support for the scientific planting model of Annona squamosa in the low-heat river valley of Yunnan Province. Full article

Cherimoya (Annona squamosa L.) is a nutrient-rich fruit. However, it is not easy to store because of its susceptibility to browning. In order to prolong the storage period of cherimoya, the fruit was treated with chlorine dioxide (ClO₂) at different concentrations (20, 40, 60, 80, and 100 mg L⁻¹) and stored at 15 °C for 8 days. The quality and biochemical indexes of the fruit were investigated using a chromameter, high-performance liquid chromatography and scanning electron microscopy, etc. The results showed that all the treatments with various concentrations of ClO₂ could delay the increase in the browning index, loss of weight, and decrease in hardness. Meanwhile, ClO₂ treatment effectively reduced the consumption of starch, titratable acids, and phenolics as well as inhibited the polyphenol oxidase (PPO) activity and enzymatic oxidation. It can be seen from the Fourier transform infrared spectrum (FTIR) that the C=O stretching peak at 1731 cm⁻¹ disappeared at a ClO₂ concentration of 60 mg L⁻¹. We think the ClO₂ treatment may inhibit the oxidation of phenol to quinone. According to the Arrhenius formula, the values of the apparent activation energy (E_a) for enzymatic browning reaction were estimated. The E_a with catechol in cherimoya pericarp and flesh were 67.00 and 47.83 kJ mol⁻¹, respectively. It was found that the phenolic enzyme reaction with catechol has a much smaller E_a and a higher affinity for PPO. Therefore, treatment with ClO₂ at a suitable concentration for cherimoya stored at 15 °C could effectively maintain fruit quality and prolong the storage period; the most appropriate concentration is 60 mg L⁻¹. Full article

Lead (Pb) is a common pollutant that is not biodegradable and gravely endangers the environment and human health. Annona squamosa fruit has a wide range of medicinal uses owing to its phytochemical constituents. This study evaluated the effect of treatment with A. squamosa fruit extract (ASFE) on testicular toxicity induced in male rats by lead acetate. The metal-chelating capacity and phytochemical composition of ASFE were determined. The LD₅₀ of ASFE was evaluated by probit analysis. Molecular docking simulations were performed using Auto Dock Vina. Forty male Sprague Dawley rats were equally divided into the following groups: Gp1, a negative control group; Gp2, given ASFE (350 mg/kg body weight (b. wt.)) (1/10 of LD₅₀); Gp3, given lead acetate (PbAc) solution (100 mg/kg b. wt.); and Gp4, given PbAc as in Gp3 and ASFE as in Gp2. All treatments were given by oro-gastric intubation daily for 30 days. Body weight changes, spermatological parameters, reproductive hormone levels, oxidative stress parameters, and inflammatory biomarkers were evaluated, and molecular and histopathological investigations were performed. The results showed that ASFE had promising metal-chelating activity and phytochemical composition. The LD₅₀ of ASFE was 3500 mg/kg b. wt. The docking analysis showed that quercetin demonstrated a high binding affinity for JAK-1 and STAT-3 proteins, and this could make it a more promising candidate for targeting the JAK-1/STAT-3 pathway than others. The rats given lead acetate had defective testicular tissues, with altered molecular, biochemical, and histological features, as well as impaired spermatological characteristics. Treatment with ASFE led to a significant mitigation of these dysfunctions and modulated the JAK-1/STAT-3/SOCS-1 axis in the rats. Full article

Recently, there has been a surge towards searching for primitive treatment strategies to discover novel therapeutic approaches against multi-drug-resistant pathogens. Endophytes are considered unexplored yet perpetual sources of several secondary metabolites with therapeutic significance. This study aims to isolate and identify the endophytic fungi from Annona squamosa L. fruit peels using morphological, microscopical, and transcribed spacer (ITS-rDNA) sequence analysis; extract the fungus’s secondary metabolites by ethyl acetate; investigate the chemical profile using UPLC/MS; and evaluate the potential antibacterial, antibiofilm, and antiviral activities. An endophytic fungus was isolated and identified as Aspergillus flavus L. from the fruit peels. The UPLC/MS revealed seven compounds with various chemical classes. The antimicrobial activity of the fungal ethyl acetate extract (FEA) was investigated against different Gram-positive and Gram-negative standard strains, in addition to resistant clinical isolates using the agar diffusion method. The CPE-inhibition assay was used to identify the potential antiviral activity of the crude fungal extract against low pathogenic human coronavirus (HCoV 229E). Selective Gram-positive antibacterial and antibiofilm activities were evident, demonstrating pronounced efficacy against both methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-sensitive Staphylococcus aureus (MSSA). However, the extract exhibited very weak activity against Gram-negative bacterial strains. The ethyl acetate extract of Aspergillus flavus L exhibited an interesting antiviral activity with a half maximal inhibitory concentration (IC₅₀) value of 27.2 µg/mL against HCoV 229E. Furthermore, in silico virtual molecular docking-coupled dynamics simulation highlighted the promising affinity of the identified metabolite, orienting towards three MRSA biotargets and HCoV 229E main protease as compared to reported reference inhibitors/substrates. Finally, ADME analysis was conducted to evaluate the potential oral bioavailability of the identified metabolites. Full article