Search Results (15)

Pineapple (Ananas comosus) is one of the most economically important fruit cultivars in South Africa. The fruit is locally consumed, processed into various industrial products or exported to foreign markets. Approximately 115,106 metric tons of pineapple fruit are harvested in South Africa. The pineapple value chain generates significant amounts of waste, in the form of pomace, peel, crown, stem, core and base. If not properly treated, pineapple waste (PAW) could have a profound detrimental impact on the environment. This calls for advanced technological platforms to transform PAW into useful bio-based products. A biorefinery is a potent strategy to convert PAW into multiple food and non-food products while effectively disposing of the waste. The objective of this review is to explore possible pathways for the valorization of PAW into energy and material products in a biorefinery. The paper looks at 10 products including biogas, biohythane, bioethanol, biobutanol, biohydrogen, pyrolytic products, single-cell proteins, animal feed, vermicompost and bioactive compounds. Several platforms (i.e., biochemical, chemical, physical and thermochemical) are available to convert PAW into valuable goods. Amongst them, the biochemical route appears to be the most favorable option for the valorization of PAW. Anaerobic digestion and fermentation are well-established biochemical technologies for PAW valorization. These methods are simple, low-cost, eco-friendly and sustainable. The focal point of emerging research is the enhanced efficacy of biorefinery platforms. The commercialization of PAW biorefining is a potential gamechanger that could revitalize the entire South African economy. Full article

An internal two-stage bioreactor constructed with a hydrogen chamber and a methane chamber with a working volume of 300 mL and 4700 mL, respectively, was operated using various kitchen waste (KW) concentrations from 10 to 80 g COD/L with a hydraulic retention time of 2 days to characterize the biomethane production performance. The results showed that daily biohythane production exhibited a similar increasing trend at KW concentrations of 10 to 40 g COD/L. The peak biomethane production was 2481 mL/day at a KW concentration of 40 g COD/L. The KW concentration could also affect the COD, carbohydrate, lipid, and protein removal efficiencies. These removal efficiencies were somehow dependent on the KW concentration, with two notable KW concentration groups of 10–20 g COD/L and 40–80 g COD/L. After 80 days of cultivation, Firmicutes dominated the hydrogen chamber, and Methanobacteriaceae and Methanomicrobiaceae dominated the methane chamber. This study presents the optimal KW concentration for high biohythane production efficiency in a novel internal two-stage bioreactor and reveals the dominant microorganisms in its microbial community. Full article

Food waste (FW) is a significant global issue with a carbon footprint of 3.3 billion tonnes (Bt), primarily generated due to improper food supply chain management, storage issues, and transportation problems. Acidogenic processes like dark fermentation, anaerobic digestion, and a combination of DF-AD can produce renewable biofuels (Bio-CH₄, Bio-H₂) by valorising FW, aligning with the UN SDGs. FW is an ideal substrate for acidogenic processes due to its high moisture content, organic matter, and biodegradability. However, the choice of FW valorisation pathways depends on energy yield, conversion efficiency, and cost effectiveness. Acidogenic processes are not economically viable for industrial scale FW treatment due to reduced energy recovery from stand-alone processes. So, this study reviews comparative studies on biogas, biohydrogen, and biohythane production from FW via acidogenic processes, focusing on energy yield, energy recovery, and environmental and economic impact to provide a clear understanding of energy recovery and yield from all acidogenic processes. Additionally, this review also explores the recent advancements in digestate slurry management and the synergistic effects of AD and HTC processes. Lastly, a futuristic integrated bio-thermo-chemical process is proposed for maximum energy recovery, valuing food waste to energy vectors (Bio-H₂, Bio-CH₄, and hydro-char) along with digestate management and biofertilizer production. Full article

The supply of waste glycerol is rising steadily, partially due to the increased global production of biodiesel. Global biodiesel production totals about 47.1 billion liters and is a process that involves the co-production of waste glycerol, which accounts for over 12% of total esters produced. Waste glycerol is also generated during bioethanol production and is estimated to account for 10% of the total sugar consumed on average. Therefore, there is a real need to seek new technologies for reusing and neutralizing glycerol waste, as well as refining the existing ones. Biotechnological means of valorizing waste glycerol include converting it into gas biofuels via anaerobic fermentation processes. Glycerol-to-bioenergy conversion can be improved through the implementation of new technologies, the use of carefully selected or genetically modified microbial strains, the improvement of their metabolic efficiency, and the synthesis of new enzymes. The present study aimed to describe the mechanisms of microbial and anaerobic glycerol-to-biogas valorization processes (including methane, hydrogen, and biohythane) and assess their efficiency, as well as examine the progress of research and implementation work on the subject and present future avenues of research. Full article

This study aims to enhance energy recovery from sugarcane leaf (SCL) through two-stage anaerobic digestion (TSAD) for hydrogen and methane production. The influence of hydraulic retention time (HRT) on this process was investigated. Optimal conditions established through batch experiments (5% total solids (TS) (w/v) and rice straw compost inoculum) were applied in semi-continuous stirred tank reactors (CSTR-H₂ and CSTR-CH₄). Remarkably, the highest production rates were achieved with HRTs of 5 days for CSTR-H₂ (60.1 mL-H₂/L·d) and 25 days for CSTR-CH₄ (238.6 mL-CH₄/L·d). Microbiological analysis by 16s rRNA sequencing identified Bacillus as predominant in CSTR-H₂ followed by Lactobacillus and Clostridium. Utilizing SCL for TSAD could reduce greenhouse gas (GHG) emissions by 2.88 Mt-CO₂ eq/year, compared to open-field burning, and mitigate emissions from fossil-fuel-based power plants by 228 kt-CO₂ eq/year. This research underscores the potential of TSAD for efficient energy recovery and significant GHG emission reductions. Full article

The current study explored bioenergy, particularly biohythane (a combination of biohydrogen (bioH₂) and biomethane (bioCH₄)), production from cow dung and untreated domestic wastewater sludge to valorize the waste into a value-added product. The experimental study consisted of a two-step process: dark fermentation (DF) and anaerobic digestion (AD) with a range of processing conditions varying the temperature and pH (acidic, neutral, and basic). The study maintained thermophilic conditions (55 °C) for bioH₂ production and mesophilic conditions (35 °C) for bioCH₄ production. The highest yields of bioH₂ and bioCH₄ were obtained at a pH of 5.5 (108.04 mL H₂/g VS) and a pH of 7.5 (768.54 mL CH₄/g VS), respectively. Microorganisms, such as Lactobacillus brevis and Clostridium butyricum, in the wastewater sludge accelerated the conversion reaction resulting in the highest bioH₂ yield for an acidic environment, while Clostridium and Bacilli enhanced bioCH₄ yield in basic conditions. The maximum cumulative yield of biohythane was obtained under basic pH conditions (pH 7.5) through DF and AD, resulting in 811.12 mL/g VS and a higher volumetric energy density of 3.316 MJ/L as compared to other reaction conditions. The experimental data were modelled using a modified Gompertz’s model at a 95% confidence interval and showed the best-fitting data from experimental and simulation results for biohythane production. The regression coefficient R² value was highly significant at 0.995 and 0.992 for bioH₂ and bioCH₄ with the change in pH during biohythane production. Thus, this study presented an effective pathway to utilize untreated domestic wastewater sludge as an inoculum, showcasing the potential of biohythane production and the generation of valuable metabolic end-products across a broad range of pH conditions. Full article

Anaerobic digestion (AD) is an environmentally friendly process for recovering low-carbon energy from the breakdown of organic substrates. In recent years, AD has undergone a major paradigm shift, and now the technology is not only considered as a “waste treatment” method and is instead viewed as a key enabler of the future “circular economy” with its potential for resource recovery (low-carbon energy, safe water, and nutrients). Currently, waste-derived biogas from AD is the most affordable and scalable source of renewable energy. Biomethane (upgraded biogas) can serve as a significant renewable and dispatchable energy source for combating the problem of global warming. Acidogenesis, an intermediate step of AD, can produce molecular hydrogen (H₂) along with green chemicals/platform chemicals. The use of low-carbon hydrogen as a clean energy source is on the rise throughout the world, and is currently considered a potential alternative energy source that can contribute to the transition to a carbon-neutral future. In order to determine the future trade routes for hydrogen, nations are developing hydrogen policies, and various agreements. Hydrogen produced by biological routes has been found to be suitable due to its potential as a green energy source that is carbon neutral for the developing “Hydrogen Economy”. Recently, hydrogen blended with methane to a specific proportion and known as biohythane/hydrogen-enriched compressed natural gas (HCNG) has emerged as a promising clean fuel that can substantially contribute to an integrated net-zero energy system. This review provides an overview of the current state of fermentative hydrogen and methane production from biogenic waste/wastewater in a biorefinery approach and its utilization in the context of energy transition. The limitations and economic viability of the process, which are crucial challenges associated with biohydrogen/biomethane production, are discussed, along with its utilization. Full article

Kitchen waste is an important component of domestic waste, and it is both harmful and rich in resources. Approximately 1.3 billion tons of kitchen waste are produced every year worldwide. Kitchen waste is high in moisture, is readily decayed, and has an unpleasant smell. Environmental pollution can be caused if this waste is treated improperly. Conventional treatments of kitchen waste (e.g., landfilling, incineration and pulverization discharge) cause environmental, economic, and social problems. Therefore, the development of a harmless and resource-based treatment technology is urgently needed. Profits can be generated from kitchen waste by converting it into biofuels. This review intends to highlight the latest technological progress in the preparation of gaseous fuels, such as biogas, biohythane and biohydrogen, and liquid fuels, such as biodiesel, bioethanol, biobutanol and bio-oil, from kitchen waste. Additionally, the pretreatment methods, preparation processes, influencing factors and improvement strategies of biofuel production from kitchen waste are summarized. Problems that are encountered in the preparation of biofuels from kitchen waste are discussed to provide a reference for its use in energy utilization. Optimizing the preparation process of biofuels, increasing the efficiency and service life of catalysts for reaction, reasonably treating and utilizing the by-products and reaction residues to eliminate secondary pollution, improving the yield of biofuels, and reducing the cost of biofuels, are the future directions in the biofuel conversion of kitchen waste. Full article

Though deemed a prospective method, the bioconversion of organic waste to biohydrogen via dark fermentation (DF) has multiple drawbacks and limitations. Technological difficulties of hydrogen fermentation may, in part, be eliminated by making DF a viable method for biohythane production. Aerobic granular sludge (AGS) is a little-known organic waste spurring a growing interest in the municipal sector; its characteristics indicate the feasibility of its use as a substrate for biohydrogen production. The major goal of the present study was to determine the effect of AGS pretreatment with solidified carbon dioxide (SCO₂) on the yield of H₂ (biohythane) production during anaerobic digestion (AD). It was found that an increasing dose of SCO₂ caused an increase in concentrations of COD, N-NH₄⁺, and P-PO₄³⁻ in the supernatant at the SCO₂/AGS volume ratios from 0 to 0.3. The AGS pretreatment at SCO₂/AGS ratios within the range of 0.1–0.3 was shown to enable the production of biogas with over 8% H₂ (biohythane) content. The highest yield of biohythane production, reaching 481 ± 23 cm³/gVS, was obtained at the SCO₂/AGS ratio of 0.3. This variant produced 79.0 ± 6% CH₄ and 8.9 ± 2% H₂. The higher SCO₂ doses applied caused a significant decrease in the pH value of AGS, modifying the anaerobic bacterial community to the extent that diminished anaerobic digestion performance. Full article

This study evaluated the feasibility of continuous biohythane production from rice straw (RS) using an integrated anaerobic bioreactor (IABR) at thermophilic conditions. NaOH/Urea solution was employed as a pretreatment method to enhance and improve biohythane production. Results showed that the maximum specific biohythane yield was 612.5 mL/g VS, including 104.1 mL/g VS for H₂ and 508.4 mL/g VS for CH₄, which was 31.3% higher than the control RS operation stage. The maximum total chemical oxygen demand (COD) removal stabilized at about 86.8%. COD distribution results indicated that 2% of the total COD (in the feed) was converted into H₂, 85.4% was converted to CH₄, and 12.6% was retained in the effluent. Furthermore, carbon distribution analysis demonstrated that H₂ production only diverted a small part of carbon, and most of the carbon flowed to the CH₄ fermentation process. Upon further energy conversion analysis, the maximum value was 166.7%, 31.7 times and 12.8% higher than a single H₂ and CH₄ production process. This study provides a new perspective on lignocellulose-to-biofuel recovery. Full article

Seaweeds represent a promising and sustainable feedstock for biofuel production which raises increasing research interests. Their high availability, easy fermentable composition, and good degradation potential make them a suitable candidate for alternating fossil fuels as an advantageous energy resource. This comprehensive review aims to summarize and discuss data from the literature on the biochemical composition of seaweeds and its potential for biomethane and biohydrogen production, as well as to investigate the effect of the common pretreatment methods. Satisfactory yields comparable to terrestrial biomass could be obtained through anaerobic digestion; concerning dark fermentation, the challenge remains to better define the operating conditions allowing a stable production of biohydrogen. Finally, we propose a potential energy production scheme with the seaweed found by the Caribbean Islands of Guadeloupe and Martinique, as well as current techno-economic challenges and future prospects. An annual energy potential of 66 GWh could be attained via a two-stage biohythane production process, this tends to be promising in terms of energetic valorization and coastal management. Full article

Spatial separation into acidogenic and methanogenic stages is considered a viable option to ensure process stability, energy efficiency and the better control of key anaerobic digestion (AD) parameters. The elucidation of the optimal modes of two-stage AD for the maximization of the recovery of biofuels (H₂ and CH₄) is still an urgent task, the main optimization criteria being the highest energy yield (EY) and energy production rate (EPR). In this work, a response surface methodology was used for an optimization of energy production from the two-stage mesophilic–thermophilic AD of cheese whey (CW). Three dilution rates of CW, providing values of 10.9, 14.53 and 21.8 g for the chemical oxygen demand (COD)/L in the influent and three hydraulic retention times (HRTs) (1, 2 and 3 days) in methanogenic biofilters at a constant HRT in an acidogenic biofilter of 0.42 days, were tested to optimize the EY and EPR. The desirability approach produced combined optimum conditions as follows: the dilution rate of the CW provided 17.58 g COD/L (corresponding to OLR of 6.5 g COD/(L·day)) in the influent and a HRT in the methanogenic biofilter of 2.28 days, both of which provided a maximum EPR of 80.263 kJ/(L·day) and EY of 9.56 kJ/g COD, with an overall desirability value of 0.883. Full article

The scope of this paper was to examine the environmental and economic performance of alternative household fermentable waste (HFW) management scenarios. In Greece, the business-as-usual scheme for the management of HFW is its disposal in landfills as part of mixed waste. Within a HORIZON2020 called Waste4think a series of alternative approaches based on the benefits of source separation was developed. Specifically, source separated HFW is led to a drying/shredding plant, located in the municipality, for the production of a high-quality biomass product, which is called FORBI (Food Residue Biomass). Alternative approaches have been examined for the exploitation of FORBI: a simple alternative consists of the transportation of food waste (without drying/shredding) to the landfill, composting and covering the landfill’s layers with the produced compost. On the other hand, a set of technological alternatives examined are: one- and two-stage anaerobic digestion for the production of biogenic compressed natural gas (bio-CNG) and bio-hythane, composting and utilization of compost in the municipality, bio-ethanol production and pelletization. The alternatives have been assessed using Life Cycle Assessment and Life Cycle Costing tools. The results show that both the simple and the innovative alternatives proposed perform better than the baseline scenario both in economic and environmental terms. Full article

Due to rapid urbanization and industrialization, the population density of the world is intense in developing countries. This overgrowing population has resulted in the production of huge amounts of waste/refused water due to various anthropogenic activities. Household, municipal corporations (MC), urban local bodies (ULBs), and industries produce a huge amount of waste water, which is discharged into nearby water bodies and streams/rivers without proper treatment, resulting in water pollution. This mismanaged treatment of wastewater leads to various challenges like loss of energy to treat the wastewater and scarcity of fresh water, beside various water born infections. However, all these major issues can provide solutions to each other. Most of the wastewater generated by ULBs and industries is rich in various biopolymers like starch, lactose, glucose lignocellulose, protein, lipids, fats, and minerals, etc. These biopolymers can be converted into sustainable biofuels, i.e., ethanol, butanol, biodiesel, biogas, hydrogen, methane, biohythane, etc., through its bioremediation followed by dark fermentation (DF) and anaerobic digestion (AD). The key challenge is to plan strategies in such a way that they not only help in the treatment of wastewater, but also produce some valuable energy driven products from it. This review will deal with various strategies being used in the treatment of wastewater as well as for production of some valuable energy products from it to tackle the upcoming future demands and challenges of fresh water and energy crisis, along with sustainable development. Full article

In recent years, production of biohydrogen and biomethane (or a mixture of these; biohythane) from organic wastes using two-stage bioreactor have been implemented by developing countries such as Germany, USA and the United Kingdom using the anaerobic digestion (AD) process. In Thailand, biohythane production in a two-stage process has been widely studied. However, in Malaysia, treating organic and agricultural wastes using an integrated system of dark fermentation (DF) coupled with anaerobic digestion (AD) is scarce. For instance, in most oil palm mills, palm oil mill effluent (POME) is treated using a conventional open-ponding system or closed-digester tank for biogas capture. This paper reviewed relevant literature studies on treating POME and other organic wastes using integrated bioreactor implementing DF and/or AD process for biohydrogen and/or biomethane production. Although the number of papers that have been published in this area is increasing, a further review is needed to reveal current technology used and its benefits, especially in Malaysia, since Malaysia is the second-largest oil palm producer in the world. Full article