Search Results (5,289)

Chronic inflammatory bowel disease, ulcerative colitis (UC), currently lacks specific drugs for clinical treatment, and screening effective therapeutic agents from natural plants represents a critical research strategy. This study aimed to investigate the therapeutic potential of the flavonoid extract of Polygonum viviparum L. (TFPV) against UC. Liquid chromatography-mass spectrometry (LC-MS) was used to identify the chemical components of TFPV, while cell and animal models were employed to evaluate its anti-inflammatory effects on lipopolysaccharide (LPS)-induced inflammation. The mechanism of anti-inflammatory action was further investigated using a mouse model of UC induced by dextran sulfate sodium (DSS). The results revealed the identification of 32 bioactive components in TFPV, with major compounds such as kaempferol, luteolin, galangin, and quercetin. TFPV effectively mitigated inflammatory damage induced by LPS in IPEC-J2 cells and C57BL/6 mice. In the UC modeled by DSS, TFPV attenuated intestinal inflammation by reducing pro-inflammatory cytokines IL-1β, IL-6, and TNF-α; increasing the anti-inflammatory cytokine IL-10; up-regulating tight junction protein expression such as Claudin-1, Occludin, and ZO-1; and inhibiting the expression of PI3K, AKT, NF-κB, and IL-17 proteins. Analysis of mice fecal samples through 16S rRNA gene sequencing demonstrated that TFPV adjusted the equilibrium of gut microbiota by boosting the abundance of Dubosiella and diminishing that of Enterococcus, Romboutsia, and Enterobacter. Untargeted metabolomics analysis further revealed that TFPV reduced inosine and ADP levels while increasing dGMP levels by the regulation of purine metabolism, ultimately resulting in decreased uric acid levels and thereby alleviating intestinal inflammation. Additionally, TFPV safeguarded the intestinal mucosal barrier by enhancing the expression of tight junctions. In conclusion, TFPV alleviates UC by blocking the PI3K/AKT/NF-κB and IL-17 signaling pathways, lessening intestinal inflammation and injury, safeguarding intestinal barrier integrity, balancing gut microbiota, and lowering uric acid levels, suggesting its promise as a therapeutic agent for UC. Full article

Introduction: Primary glomerulopathies are immune-driven kidney diseases. IgA nephropathy (IgAN) and membranoproliferative glomerulonephritis (MPGN) are prevalent entities with a risk of chronic progression. Immune checkpoints, such as PD-1/PD-L1, CTLA-4/CD86, and CD200R/CD200, regulate activation and tolerance in T, B, and NK cells, and also exist in soluble forms, reflecting systemic immune balance. Objective: To compare immune checkpoint profiles in IgAN and MPGN versus healthy volunteers (HV) through surface expression, soluble serum levels, and PBMC transcripts, with attention to sex-related differences and diagnostic value assessed by ROC curves. Materials and Methods: Ninety age-matched subjects were studied: IgAN (n = 30), MPGN (n = 30), HV (n = 30). Flow cytometry evaluated checkpoint expression on CD4+/CD8+ T cells, CD19+ B cells, and NK cells. ELISA quantified sPD-1, sPD-L1, sCTLA-4, sCD86, sCD200, sCD200R; PBMC transcript levels were assessed. Group comparisons, sex stratification, and ROC analyses were performed. Results: Lymphocyte distributions were preserved, but IgAN patients showed anemia and impaired renal function, while MPGN patients had greater proteinuria and dyslipidemia. GN patients displayed increased PD-1/PD-L1 and CD200R/CD200, with reduced CTLA-4/CD86, compared to HV. Serum analysis revealed elevated sPD-1, sPD-L1, sCD200, sCD200R and decreased sCTLA-4, sCD86. PBMC transcripts paralleled these trends, with PD-1/PD-L1 mainly increased in MPGN. Sex had minimal impact. ROC analyses showed strong GN vs. HV discrimination by CD19⁺CTLA-4⁺, PD-1/PD-L1, and CD200/CD200R, but limited ability to separate IgAN from MPGN. Conclusions: IgAN and MPGN share a sex-independent checkpoint signature: PD-1/PD-L1 and CD200R/CD200 upregulation with CTLA-4/CD86 downregulation. CD19⁺, CTLA-4⁺, and soluble PD-1/PD-L1/CD200(R) emerge as promising biomarkers requiring further validation. Full article

With the rapid advancement of energy storage technologies, there is a growing demand for affordable, efficient, and environmentally benign battery systems. Sodium-ion batteries (SIBs) present a promising alternative to lithium-ion systems due to sodium’s high abundance and similar electrochemical properties. Particular attention is given to developing NASICON -sodium (Na) super ionic conductor, type cathode materials, especially Na3V2(PO4)3, which exhibits high thermal and structural stability. This study focuses on the sol–gel synthesis of Na₃V₂(PO₄)₃ using citric acid and ethylene glycol, as well as investigating the effect of annealing temperature (400–1000 °C) on its structural and electrochemical properties. Phase composition, morphology, textural characteristics, and electrochemical performance were systematically analyzed. Above 700 °C, a highly crystalline NASICON phase free of secondary impurities was formed, as confirmed by X-ray diffraction (XRD). Microstructural evolution revealed a transition from a loose amorphous structure to a dense granular morphology, accompanied by changes in specific surface area and porosity. The highest surface area (67.40 m²/g) was achieved at 700 °C, while increasing the temperature to 1000 °C caused pore collapse due to sintering. X-ray photoelectron spectroscopy (XPS) confirmed the predominant presence of V³⁺ ions and the formation of V⁴⁺ at the highest temperature. The optimal balance of high crystallinity, uniform elemental distribution, and stable texture was achieved at 900 °C. Electrochemical testing in a Na/NVP half-cell configuration delivered an initial capacity of 70 mAh/g, which decayed to 55 mAh/g by the 100th cycle, attributed to solid-electrolyte interphase (SEI) formation and irreversible Na⁺ trapping. These results demonstrate that the proposed approach yields high-quality Na₃V₂(PO₄)₃ cathode materials with promising potential for sodium-ion battery applications. Full article

For the first time, the effect of low-temperature vacuum drying (LTVD) on wild murta (Ugni molinae Turcz) was evaluated, in comparison with freeze-drying (FD) and vacuum drying (VD), to assess their capacity to preserve bioactive compounds and associated bioactivities. Murta was dried using LTVD at 20, 30, and 40 °C under a constant vacuum of 10 mbar, where FD and VD at 60 °C (VD 60) were included as comparative methods. The content of fatty acids and tocols, along with the retention of bioactive compounds and their antioxidant, anti-inflammatory, cytotoxic, and α-glucosidase inhibitory activities, were systematically analyzed. LTVD- and VD-dried murta exhibited higher polyunsaturated-to-saturated fatty acid ratios (>9.0) and markedly greater tocol contents, whereas FD maintained a more balanced ratio (<5.0) but with lower tocol levels. While FD was most effective in preserving catechin, higher levels of other phenolic compounds were observed in samples dried by LTVD at 20 and 40 °C, as well as VD 60, possibly due to the release of bound forms during processing. The drying method significantly influenced murta bioactivity. LTVD 30 preserved the highest antioxidant capacity, while topical anti-inflammatory effects on skin lesions varied by pathway, with LTVD 40 being the most effective in the TPA model and FD in the AA model. These effects were evaluated only using a topical inflammation model in BALB/c mice of both sexes; dietary effects were not assessed in this study. Regarding other bioactivities, VD 60 extracts excelled in both cytotoxic and α-glucosidase inhibitory effects, whereas FD extracts were the most effective against AGS cells and LTVD 20 against α-glucosidase. In conclusion, LTVD emerges as a promising alternative to FD and VD, showing potential to preserve bioactive compounds and key bioactivities of wild murta, although further studies are needed to elucidate the underlying mechanisms. Full article

Adipose tissue renewal requires precise coordination of stem/progenitor cell proliferation, preadipocyte commitment, and terminal adipocyte differentiation. T-cadherin (CDH13), an atypical GPI-anchored cadherin, is expressed in adipose tissue and functions as a receptor for high-molecular-weight (HMW) adiponectin—a key adipokine produced by adipose tissue and involved in metabolic regulation. While T-cadherin is implicated in cardiovascular and metabolic homeostasis, its role in adipogenesis still remains poorly understood. In this study, we used the 3T3-L1 preadipocyte model to investigate the function of T-cadherin in adipocyte differentiation. We analyzed T-cadherin expression dynamics during differentiation and assessed how T-cadherin overexpression or knockdown affects lipid accumulation, expression of adipogenic markers, and key signaling pathways including ERK, PI3K–AKT, AMPK, and mTOR. Our findings demonstrate that T-cadherin acts as a negative regulator of adipogenesis. T-cadherin overexpression ensured a proliferative, undifferentiated cell state, delaying early adipogenic differentiation and suppressing both lipid droplet accumulation and the expression of adipogenic markers. In contrast, T-cadherin downregulation accelerated differentiation, enhanced lipid accumulation, and increased insulin responsiveness, as indicated by PI3K–AKT pathway activation at specific stages of adipogenesis. These results position T-cadherin as a key modulator of adipose tissue plasticity, regulating the balance between progenitor expansion and terminal differentiation, with potential relevance to obesity and metabolic disease. Full article

This study focuses on the preparation of poly(HCQM-co-VP) copolymeric nanoparticles (NPs) to enhance the aqueous solubility and bioavailability of the hydrophobic and antitumor molecules HCQ (hydroxychloroquine) and α-TOS (α-tocopheryl succinate). HCQ is covalently incorporated into the polymer backbone, while α-TOS is encapsulated within the nanoparticles by non-covalent interactions. Poly(HCQM-co-VP) was synthesized from a vinyl derivative of HCQ (HCQM) and N-vinylpyrrolidone (VP), with a molar composition of 17% HCQM and 83% VP, providing the optimal hydrophobic/hydrophilic balance for forming, via nanoprecipitation, empty nanoparticles (NPs) with a diameter of 123.6 nm and a zeta potential of −5.8 mV. These nanoparticles effectively encapsulated α-TOS within their hydrophobic core, achieving an encapsulation efficiency (%EE) of 78%. These α-TOS-loaded NPs resulted in smaller diameters and more negative zeta potentials (71 nm, −19.2 mV) compared to the non-loaded NPs. The cytotoxicity of these NPs was evaluated using the AlamarBlue assay on MCF-7 breast cancer cells. The empty NPs showed no toxic effects within the tested concentration range, after 72 h of treatment. In contrast, the α-TOS-loaded NPs, exhibited a pronounced cytotoxic effect on MCF-7 cells with an IC₅₀ value of 100.2 μg·mL⁻¹, thereby demonstrating their potential as controlled drug delivery systems for cancer treatment. These findings contribute to the development of a new HCQ-based polymeric nanocarrier for α-TOS or other hydrophobic drugs for the treatment of cancer and other diseases treatable with these drugs. Full article

The increasing use of UAVs has reshaped urban logistics, enabling sustainable alternatives to traditional deliveries. To address critical issues inherent in the system, the proposed study presents the design and evaluation of an innovative unmanned aerial vehicle (UAV) prototype that integrates advanced electronic components and artificial intelligence (AI), with the aim of reducing environmental impact and enabling autonomous navigation in complex urban environments. The UAV platform incorporates brushless DC motors, high-density LiPo batteries and perovskite solar cells to improve energy efficiency and increase flight range. The Deep Q-Network (DQN) allocates energy and selects reference points in the presence of wind and payload disturbances, while an integrated sensor system monitors motor vibration/temperature and charge status to prevent failures. In urban canyon and field scenarios (wind from 0 to 8 m/s; payload from 0.35 to 0.55 kg), the system reduces energy consumption by up to 18%, increases area coverage by 12% for the same charge, and maintains structural safety factors > 1.5 under gust loading. The approach combines sustainable materials, efficient propulsion, and real-time AI-based navigation for energy-conscious flight planning. A hybrid methodology, combining experimental design principles with finite-element-based structural modelling and AI-enhanced monitoring, has been applied to ensure structural health awareness. The study implements proven edge-AI sensor fusion architectures, balancing portability and telemonitoring with an integrated low-power design. The results confirm a reduction in energy consumption and CO₂ emissions compared to traditional delivery vehicles, confirming that the proposed system represents a scalable and intelligent solution for last-mile delivery, contributing to climate resilience and urban sustainability. The findings position the proposed UAV as a scalable reference model for integrating AI-driven navigation and renewable energy systems in sustainable logistics. Full article

Background: Early detection of dysglycemia in young adults is important but underexplored. This study aimed to (1) predict long-term changes in fasting plasma glucose (δ-FPG) and (2) classify future prediabetes using complementary machine learning (ML) approaches. Methods: We analyzed 6247 Taiwanese men aged 18–35 years (mean follow-up 5.9 years). For δ-FPG (continuous outcome), random forest, stochastic gradient boosting (SGB), eXtreme gradient boosting (XGBoost), and elastic net were compared with multiple linear regression using Symmetric mean absolute percentage error (SMAPE), Root mean squared error (RMSE), Relative absolute error(RAE), and Root relative squared error (RRSE) Sensitivity analyses excluded baseline FPG (FPG_base). Shapley additive explanations(SHAP) values provided interpretability, and stability was assessed across 10 repeated train–test cycles with confidence intervals. For prediabetes (binary outcome), an XGBoost classifier was trained on top predictors, with class imbalance corrected by SMOTE-Tomek. Calibration and decision-curve analysis (DCA) were also performed. Results: ML models consistently outperformed regression on all error metrics. FPG_base was the dominant predictor in full models (100% importance). Without FPG_base, key predictors included body fat, white blood cell count, age, thyroid-stimulating hormone, triglycerides, and low-density lipoprotein cholesterol. The prediabetes classifier achieved accuracy 0.788, precision 0.791, sensitivity 0.995, ROC-AUC 0.667, and PR-AUC 0.873. At a high-sensitivity threshold (0.2892), sensitivity reached 99.53% (specificity 47.46%); at a balanced threshold (0.5683), sensitivity was 88.69% and specificity was 90.61%. Calibration was acceptable (Brier 0.1754), and DCA indicated clinical utility. Conclusions: FPG_base is the strongest predictor of glycemic change, but adiposity, inflammation, thyroid status, and lipids remain informative. A dual interpretable ML framework offers clinically actionable tools for screening and risk stratification in young men. Full article

Titanium and its alloys are widely used in biomedical implants due to their favorable mechanical properties and corrosion resistance; however, their natural surface lacks sufficient bioactivity and antibacterial performance. Micro-arc oxidation is a promising approach to producing bioactive coatings, and the incorporation of nanoparticles such as TiO₂ may further improve their functionality. This study aimed to determine the optimal TiO₂ nanoparticle concentration in the micro-arc oxidation electrolyte that ensures coating stability and biological safety. Calcium–phosphate coatings were fabricated on commercially pure titanium using micro-arc oxidation with two TiO₂ concentrations: 0.5 wt.% (MAO 1) and 1 wt.% (MAO 2). Surface morphology, porosity, and phase composition were analyzed by scanning electron microscopy, energy-dispersive spectroscopy, and X-ray diffraction. Corrosion resistance was evaluated via potentiodynamic polarization in NaCl and Ringer’s solutions, while biocompatibility was assessed in vitro using HOS human osteosarcoma cells and MTT assays. Increasing the TiO₂ content to 1% decreased coating porosity (13.7% vs. 26.3% for MAO 1), enhanced corrosion protection, and reduced the friction coefficient compared to bare titanium. However, MAO 2 exhibited high cytotoxicity (81% cell death) and partial structural degradation in the biological medium. MAO 1 maintained integrity and showed no toxic effects (3% cell death). These results suggest that 0.5% TiO₂ is the optimal concentration, providing a balance between corrosion resistance, mechanical stability, and biocompatibility, supporting the development of safer implant coatings. Full article

Battery cell balancing is crucial in series-connected lithium-ion packs to maximize usable capacity, ensure safe operation, and prolong cycle life. This paper presents a comprehensive study and a novel adaptive duty-cycled hybrid balancing system that combines passive bleed resistors and an active switched-capacitor (SC) balancer, specifically designed for a 4-cell series-connected battery pack. This work also explored open circuit voltage (OCV)-driven adaptive pulse-frequency modulation (PFM) active balancing to achieve higher efficiency and better balancing speed based on different system requirements. Finally, this paper compares passive, active (SC-based), and adaptive duty-cycled hybrid balancing strategies in detail, including theoretical modeling of energy transfer and efficiency for each method. Simulation showed that the adaptive hybrid balancer speeds state-of-charge (SoC) equalization by 16.24% compared to active-only balancing while maintaining an efficiency of 97.71% with minimal thermal stress. The simulation result also showed that adaptive active balancing was able to achieve a high efficiency of 99.86% and provided an additional design degree of freedom for different applications. The results indicate that the adaptive hybrid balancer offered an excellent trade-off between balancing speed, efficiency, and implementation simplicity for 4-cell Li-ion packs, making it highly suitable for applications such as high-voltage portable chargers. Full article

Biodegradable plastics are not a primary solution to plastic pollution, and empirical evidence on whether they are environmentally friendly remains lacking. In this study, we systematically compared the toxic effects of traditional microplastics (polypropylene, PP; polystyrene, PS) with biodegradable microplastics (polylactic acid, PLA; polyhydroxyalkanoates, PHA) on the haplic phaeozem ecosystem. Through mathematical modeling analysis, it was found that earthworms initially rely on antioxidant enzymes to resist stress, mid-term activation of detoxifying enzymes to repair damage, and maintaining physiological balance through metabolic regulation and immune enhancement in later stages. We elucidated their mechanism differences: PLA and PP caused severe damage to the antioxidant system and cell membrane, with PLA mainly relying on POD to clear peroxides and PP relying on GST. In addition, PLA and PS can induce early neurotoxicity (AChE), while PHA induces late neurotoxicity. Furthermore, this study provides direct evidence proving that biodegradable microplastics are not environmentally friendly by breaking through the one-way research framework of “microplastic biotoxicity” and innovatively constructing a path analysis model that links biological physiological responses with soil ecological functions. We also provide a scientific basis to evaluate the ecological risks of microplastic pollution in soil and the whether biodegradable plastics are truly environmentally friendly. Full article

Introduction: Direct pulp capping (DPC) aims to preserve the vitality of the dental pulp by placing a protective biocompatible material over the exposed pulp tissue to facilitate healing. There are several calcium-silicate materials that have been designed to promote mineralization and the regulation of inflammation. These have strong potential for the repair and regeneration of dental pulp. Among them, Biodentine (BD) and EndoSequence RRM Putty (ES) have been found to promote in vitro and in vivo mineralization while minimizing some of the limitations of the first-generation calcium-silicate-based materials. Theracal-LC (TLC), a light-cured, resin-modified calcium-silicate material, is a newer product with potential to improve the clinical outcomes of DPC, but existing studies have reported conflicting findings regarding its biocompatibility and ability to support pulpal healing in direct contact with the pulp. A comprehensive assessment of the biocompatibility and pulpal protection provided by these three capping materials has not yet been performed. Aim: We aimed to quantify the inflammatory response, dentin bridge formation, and material adaptation following DPC using three calcium-silicate materials: ES, BD, and TLC. Materials and Methods: DPC was performed on the maxillary first molar of C57BL/6 female mice. Maxilla were collected and processed at 1 and 21 days post-DPC. The early inflammatory response was measured 24 h post-procedure using confocal imaging of anti-Lys6G6C, which indicates the extent of neutrophil and monocyte infiltration. Reparative mineralized bridge formation was assessed at 21 days post-procedure using high-resolution micro-computed tomography (micro-CT) and histology. Lastly, the homogeneity of the capping materials was evaluated by quantifying voids in calcium-silicate restorations using micro-CT. Results: DPC using TLC induced less infiltration of Lys6G6C⁺ cells at 24 h than BD or ES. BD promoted higher volumes of tertiary dentin than TLC, but TLC and ES showed no significant differences in volume. No differences were observed in material adaptation and void spaces among the three capping materials. Conclusions: All three materials under investigation supported pulp healing and maintained marginal integrity. However, TLC induced a lower inflammatory response on day 1 and induced similar levels of tertiary dentin to ES. These observations challenge the common perception that resin-based capping materials are not suitable for direct pulp capping. Our findings underscore the need to balance biological responses with physical properties when selecting pulp capping materials to improve long-term clinical success. Full article

This paper proposes hybrid hydrogel insulin carriers based on alginate-hyaluronan (ALG/HA) and hydroxypropyl methylcellulose-hyaluronan (HPMC/HA) for topical application. The inclusion of insulin in a modern dressing can help restore metabolic balance and proper cell signaling in diseased tissue. Preformulation studies of the developed preparations were conducted, including analysis of the in vitro pharmaceutical availability of insulin, rotational and oscillatory rheology tests, and texture profile analysis. It was found that the developed insulin formulations provide an acceptable compromise between rheological and textural properties and ease of application, while ensuring prolonged release of the active substance. The results obtained provide a basis for further preclinical and clinical studies. Full article

Background: Temporal lobe epilepsy (TLE), a prevalent refractory focal epilepsy frequently complicated by comorbid anxiety and depression, poses significant therapeutic challenges due to the inadequate efficacy of current antiepileptic drugs in seizure control. Carbon dots (CDs) demonstrate notable biological activities and represent a promising class of nanomedicines for TLE intervention. Methods: This study established an eco-friendly calcination protocol to synthesize a novel suspension of Crinis Carbonisatus-derived carbon dots (CC-CDs) as a candidate therapeutic for TLE. Results: In a TLE mouse model, the CC-CDs suspension significantly inhibited phosphorylation of the MAPK pathway (p-JNK, p-ERK, p-p38; p < 0.01, p < 0.05), leading to reduced levels of pro-inflammatory cytokines (IL-6, IL-1β, TNF-α; p < 0.01, p < 0.05), upregulation of TGF-β1 (p < 0.01, p < 0.05), and restoration of antioxidant enzyme activities (SOD, GSH, CAT; p < 0.01, p < 0.05). These modifications subsequently regulated the Glu/GABA balance, alleviating excitotoxicity (p < 0.05), attenuating neuronal damage and Nissl body loss in hippocampal CA1/CA3 regions, and improving cognitive function alongside reducing anxiety-like behaviors (p < 0.01, p < 0.05). In vitro, the CC-CDs suspension suppressed LPS-induced apoptosis in BV2 cells. Conclusions: The CC-CDs suspension ameliorates TLE by inhibiting MAPK signaling, thereby reducing neuroinflammation and oxidative stress, rectifying Glu/GABA imbalance, attenuating excitotoxicity, and ultimately improving behavioral deficits. These findings underscore the therapeutic potential of CC-CDs suspension for TLE treatment. Full article

We explore a plasmonic interface for perovskite solar cells (PSCs) by integrating inkjet-printed TiO₂-AuNP microdot arrays (MDA) into the electron transport layer. This systematic study examines how the TiO₂ blocking layer (BL) surface conditioning, AuNP layer positioning, and nanoparticle loading collectively influence device performance. Pre-annealing the BL increases its hydrophobicity, yielding smaller and denser AuNP microdots with an enhanced localized surface plasmon resonance (LSPR). Positioning the AuNP MDA at the BL/perovskite interface (above the BL) maximizes near-field plasmonic coupling to the absorber, resulting in higher photocurrent and power conversion devices; these trends are corroborated by finite-difference time-domain (FDTD) simulations. Moreover, these devices demonstrate better stability over time compared to those with AuNPs at the transparent electrode (under BL). Although higher AuNP concentrations improve dispersion stability, preserve MAPI crystallinity, and yield more uniform nanoparticle sizes, device measurements showed no performance gains. After annealing, the samples with the Au content of 23 wt% relative to TiO₂ achieved optimal PSC efficiency by balancing plasmonic enhancement and charge transport without the increased resistance and recombination losses seen at higher loadings. Importantly, X-ray diffraction (XRD) confirms that introducing the TiO₂-AuNP MDA at the interface does not disrupt the perovskite’s crystal structure, underscoring the structural compatibility of this plasmonic enhancement. Overall, our findings highlight a scalable strategy to boost PSC efficiency via engineered light-matter interactions at the nanoscale without compromising the perovskite’s structural integrity. Full article