Search Results (5,110)

Regenerative agricultural practices emphasize the use of diverse pasture species within sustainable agriculture production systems. The inclusion of a range of legume species in diverse pasture swards is likely to increase biological N fixation (BNF) across seasons, reducing the system’s reliance on synthetic N inputs. The present field study aims to quantify BNF in selected legume species within diverse pasture (combining 9 species) and standard pastures (ryegrass and clover combination) and assess their performance to identify the potential for improving N supply while maintaining year-round pasture quality. A year-round seasonal BNF was assessed by evaluating soil N status, nodulation patterns, plant composition, and conducting ¹⁵N natural abundance studies. The results revealed that the diverse pasture sward produced 5.4% more dry matter compared to the standard pasture, while soil mineral N (NO₃⁻, NH₄⁺) remained statistically similar between the two treatments. Nitrogen yield was 9.3% higher in the diverse pasture than in the standard pasture. ¹⁵N natural abundance analysis assessment revealed no substantial variation in BNF rates across treatments throughout the study. However, in contrast to standard pasture, the BNF rate in diverse pasture experienced a 3-fold increase from winter to summer, while the standard pasture exhibited a 1.5-fold increase. In both pasture systems, BNF increased with clover proportion up to 30%, indicating optimal fixation at moderate clover levels. The findings underscore the potential of diverse pastures when strategically managed to enhance seasonal BNF while sustaining pasture productivity. Full article

Time series clustering plays a vital role in various analytical and pattern recognition tasks by partitioning structurally similar sequences into semantically coherent groups, thereby facilitating downstream analysis. However, building high-quality clustering models remains challenging due to three key issues: (i) capturing dynamic shape variations across sequences, (ii) ensuring discriminative cluster structures, and (iii) enabling end-to-end optimization. To address these challenges, we propose KDiscShapeNet, a structure-aware clustering framework that systematically extends the classical k-Shape model. First, to enhance temporal structure modeling, we adopt Kolmogorov–Arnold Networks (KAN) as the encoder, which leverages high-order functional representations to effectively capture elastic distortions and multi-scale shape features of time series. Second, to improve intra-cluster compactness and inter-cluster separability, we incorporate a dual-loss constraint by combining Center Loss and Supervised Contrastive Loss, thus enhancing the discriminative structure of the embedding space. Third, to overcome the non-differentiability of traditional K-Shape clustering, we introduce Differentiable k-Shape, embedding the normalized cross-correlation (NCC) metric into a differentiable framework that enables joint training of the encoder and the clustering module. We evaluate KDiscShapeNet on nine benchmark datasets from the UCR Archive and the ETT suite, spanning healthcare, industrial monitoring, energy forecasting, and astronomy. On the Trace dataset, it achieves an ARI of 0.916, NMI of 0.927, and Silhouette score of 0.931; on the large-scale ETTh1 dataset, it improves ARI by 5.8% and NMI by 17.4% over the best baseline. Statistical tests confirm the significance of these improvements (p < 0.01). Overall, the results highlight the robustness and practical utility of KDiscShapeNet, offering a novel and interpretable framework for time series clustering. Full article

The European e-Competence Framework (e-CF) and the European Skills, Competences, Qualifications and Occupations (ESCO) classification are two key initiatives developed by the European Commission to support skills transparency, mobility, and interoperability across labour and education systems. While e-CF defines essential competences for ICT professionals through a structured framework, it provides only a limited number of illustrative skills and knowledge examples for each competence. In contrast, ESCO offers a rich, multilingual taxonomy of skills and knowledge, each accompanied by a detailed description, alternative labels, and links to relevant occupations. This paper explores the possibility of enriching the e-CF framework by linking it to relevant ESCO ICT skills using text embedding (MPNet) and cosine similarity. This approach allows the extension to 15–25 semantically aligned skills and knowledge items per competence in e-CF, all with full description and officially translated into all EU languages, instead of the present amount of 4–10 brief examples. This significantly improves the clarity, usability, and interpretability of e-CF competences for the various stakeholders. Furthermore, since ESCO terminology serves as the foundation for labour market analysis across the EU, establishing this linkage provides a valuable bridge between the e-CF competence model and real-time labour market intelligence, a connection not available now. The results of this study offer practical insights into the application of semantic technologies to the enhancement and mutual alignment of European ICT skills frameworks. Full article

WRKY transcription factors play an important role in transcriptional reprogramming associated with plant abiotic stress responses. In this study, the role of Solanum tuberosum (S. tuberosum; St) WRKY transcription factors StWRKY4 and StWRKY56 were explored in response to salt stress by generating transgenic potato lines using RNAi. The results showed that the total chlorophyll content in transgenic StWRKY4 was 6.1 mg/g at 200 mM after 35 days; however, in StWRKY56, an elevated 12.6 mg/g total chlorophyll was observed which indicated different operating mechanisms of these StWRKY transcription factors under salt stress. Proline content increased to 1.0 mg/g in StWRKY4 while it decreased to 0.54 mg/g in StWRKY56 as compared to their respective control plants after 35 days at 200 mM of salt stress. For Na⁺/K⁺ ratios, StWRKY4 and StWRKY56 showed 32.3 and 5.5 values, respectively, in silenced plants under similar conditions. This shows contrasting trends in StWRKY4 and StWRKY56 for Na⁺/K⁺. However, the expression analyses of StSOS1s were found to be upregulated, whereas for StNHX3s these were found to be downregulated in StWRKY4 and StWRKY56 under salt stress. Thus, this study, for the first time, demonstrated the different but critical roles of StWRKY4 and StWRKY56 for fine-regulating salt stress tolerance in complex signaling network of potato plant. Full article

WWOX and HIF1α proteins are involved in cancer progression; their functions are closely related. WWOX binds HIF1α through its WW domains, sequestering it in the cytoplasm and inhibiting its transcriptional activity. This study evaluates the prognostic significance of the WWOX/HIF1A interaction across cancers, breast cancer subtypes, glioblastoma (GBM), low-grade glioma (LGG), and hepatocellular carcinoma (HCC) through gene expression and pathway analysis focused on metabolism, ECM, and epithelial–mesenchymal transition. In breast cancer, metabolic pathways correlated with good prognosis in basal subtypes. HER2 subtypes showed enrichment in DNA replication pathways. Luminal A subtypes showed favourable prognosis via TNF and PI3K/AKT signalling, while luminal B subtypes had poor prognosis tied to metabolic activity; genes associated with good prognosis mirrored those tied to poor prognosis in luminal A. In HCC, enhanced metabolic activity was associated with good prognosis. In contrast, poor prognosis involved TNF signalling and cytoskeleton-related pathways, indicating more aggressive tumour behaviour. In LGG, good prognosis was linked to metabolic and cAMP pathways, while poor outcomes involved TNF, cell cycle, apoptosis, and focal adhesion pathways. GBM showed similar patterns: metabolic and cAMP pathways indicated better outcomes, while NFKB, TNF, JAK-STAT, and PI3K/AKT pathways marked poor prognosis. These findings suggest the WWOX/HIF1A ratio is a robust prognostic marker and a possible guide for developing targeted treatments. Full article

Selection indices enhance dairy cattle breeding by optimizing multiple traits simultaneously. This study analyzed 2181 lactation records from Holstein–Friesian cows at Sakha Experimental Farm, Egypt, to evaluate selection indices for 305-day milk yield (MY), fat yield (FY), and protein yield (PY) using two relative economic value (REV) methods: actual economic values (REV1) and one phenotypic standard deviation (REV2). Using MTDFREML software, we estimated heritabilities of 0.27 ± 0.01 (MY), 0.22 ± 0.01 (FY), and 0.28 ± 0.02 (PY). Four selection indices were constructed based on actual relative economic values (REV1) and one phenotypic standard deviation (REV2). The comprehensive index (I₁) incorporated all three key production traits, viz., MY, FY, and PY, to maximize the genetic merit of the aggregate genotype. In contrast, the reduced indices (I₂, I₃, and I₄) included only two traits each. The I₂ incorporated MY and FY, the I₃ included MY and PY, and the I₄ included FY and PY. The index I₁ (including all traits) yields the highest genetic gains (305 kg MY, 14.0 kg FY, 11.93 kg PY per generation). Both REV methods produced comparable genetic gains, but REV2 is recommended for its computational simplicity. These findings support the use of selection indices for genetic improvement in Holstein–Friesian cows, offering practical guidance for dairy breeding programs in Egypt and similar environments. Full article

Background and Clinical Significance: Patients with a neurodegenerative condition known as posterior cortical atrophy (PCA) can present with attention impairments across a variety of cognitive contexts, but the consequences of these are little explored in example of single word reading. Case Presentation: We present a detailed single-case study of KL, a local resident of South Wales, a patient diagnosed with posterior cortical atrophy (PCA) in 2018, whose reading and letter-naming abilities are selectively disrupted under non-canonical visual presentations. In particular, KL shows significantly impaired accuracy performance when reading words presented in tilted (rotated 90°) format. By contrast, his reading under conventional horizontal (canonical) presentation is nearly flawless. Whilst other presentation formats including, mixed-case text (e.g., TaBLe) and vertical (marquee) format led to only mild performance decrements—even though mixed-case formats are generally thought to increase attentional ‘crowding’ effects. Discussion: These findings indicate that impairments of word reading can emerge in PCA when visual-attentional demands are sufficiently high, and access to ‘top down’ orthographic information is severely attenuated. Next, we explored a cardinal feature of attentional dyslexia, namely the word–letter reading dissociation in which word reading is superior to letter-in-string naming. In KL, a similar dissociative pattern could be provoked by non-canonical formats. That is, conditions that similarly disrupted his word reading led to a pronounced disparity between word and letter-in-string naming performance. Moreover, different orientation formats revealed the availability (or otherwise) of distinct compensatory strategies. KL successfully relied on an oral (letter by letter) spelling strategy when reading vertically presented words or naming letters-in-strings, whereas he had no ability to engage compensatory mental rotation processes for tilted text. Thus, the observed impact of non-canonical presentations was moderated by the success or failure of alternative compensatory strategies. Conclusions: Importantly, our results suggest that an attentional ‘dyslexia-like’ profile can be unmasked in PCA under sufficiently taxing visual-attentional conditions. This approach may prove useful in clinical assessment, highlighting subtle reading impairments that conventional testing might overlook. Full article

This study focuses on the detailed investigation of the eutectic Aluminium Silicon (Al-12.6 wt% Si) alloy, which was solidified without and with a 10 mT induction rotating magnetic field (RMF). The experiments were conducted as part of the MICAST Hungary project, as the mirror experiments were solidified in the Solidification and Quenching Furnace (SQF) at the International Space Station (ISS). The mirror samples were solidified using solidification parameters similar to the ISS experiments. This study examined the meso-structure of the samples and the eutectic microstructure in both stirred (RMF-applied) and non-stirred (RMF-free) samples. Special attention was given to the influence of magnetic stirring on key microstructural features, such as the eutectic lamellae distance, the length of the lamellae, and the spatial orientation of the lamellae were investigated. Measuring and analysing these parameters gives us an overall picture of the microstructure of the eutectics. The 10 mT low-intensity RMF used in the experiment has a demonstrable effect on the formation of the eutectic structure; short aluminium dendrites concentrate at both edges of the stirred sample, and their proportion decreases as the sample approaches its end. In contrast, in the non-stirred sample, long, elongated Al dendrites solidify parallel to the direction of heat removal, and their proportion and size continuously increase as the sample progresses. Furthermore, a possible relationship was found between the decrease in the eutectic lamella length and the lamellae’s average distance. Full article

This study evaluated the effects of red and blue laser irradiation on the development and reproduction of the Asian corn borer (Ostrinia furnacalis (Guenée)) under controlled laboratory conditions, aiming to explore its potential for non-chemical pest control. Larvae were exposed to laser light at different wavelengths and intensities, and key biological parameters—including egg hatching, larval duration, pupation, adult emergence, and oviposition—were assessed. Red laser light slightly delayed egg hatching but had minimal effects on subsequent developmental stages. In contrast, blue laser irradiation significantly prolonged the larval period and reduced pupation rates. Combined red–blue treatments produced similar inhibitory effects to blue light alone, suggesting that blue wavelengths were the primary factor driving developmental delays. These findings demonstrate that blue and red–blue laser irradiation can effectively interfere with the life cycle of O. furnacalis, offering a promising approach for sustainable, light-based pest management strategies. Full article

This paper investigates the associations between urban form, neighbourhood characteristics, socioeconomic factors and commuting mode choice and neighbourhood-level active travel (walking and cycling) in Windhoek, Namibia. Despite growing interest in sustainable mobility, limited research has examined these relationships in medium-sized African cities, particularly in distinguishing between commuting and neighbourhood travel behaviour. To address this gap, the study explores three interrelated research questions: (1) In what ways are urban form, accessibility, and socioeconomic factors associated with residents’ choices between motorised and non-motorised commuting modes? (2) What factors determine the propensity of cycling within neighbourhoods? (3) How are similar factors associated with walking propensity at the neighbourhood level? Using survey data from 1000 residents across nine constituencies and spatial analysis through GIS, the study applies binary logistic and multiple linear regression models to analyse commuting and local travel patterns. The findings show that commuting mode choice is significantly associated with socioeconomic status, car ownership, commuting time, and urban sprawl around homes, all of which reduce the likelihood of walking or cycling. Neighbourhood walking, in contrast, is largely driven by necessity in underserved, high-density areas and is positively associated with population density, perceived safety, and community belonging but constrained by inadequate infrastructure and car access. Cycling, though less frequent, is associated with perceived security, access to local amenities, and cycling competence, while negatively constrained by inexperience and cultural norms. The study concludes that fragmented urban form and socioeconomic disparities reinforce mobility exclusion and calls for equity-oriented transport planning that integrates infrastructure and behavioural change. Full article

Background/Objectives: Low levels of lysophosphatidylcholine (LPC) in the blood can be used as a diagnostic marker for sepsis. SARS-CoV-2 infection, a more recent cause of sepsis, shares similarities with non-SARS-CoV-2 sepsis but also exhibits distinct features. We have recently shown that plasma cholesteryl ester levels are higher in patients with SARS-CoV-2 infection than in patients without, and this study analysed whether this may extend to differences in LPC, a bioactive constituent of lipoproteins. Methods: The plasma levels of 13 LPC species were measured by flow injection analysis tandem mass spectrometry (FIA-MS/MS) in 157 patients with systemic inflammatory response syndrome (SIRS), sepsis or septic shock. Of these patients, 24 had SARS-CoV-2 infection. Results: Patients with SIRS exhibited higher plasma levels of the minor LPC species LPC 15:0 and 22:4 compared to those with sepsis or septic shock. Five LPC species were also reduced in the plasma of 31 patients with liver cirrhosis; therefore, patients with cirrhosis or SIRS were excluded from subsequent analyses. Compared to 76 non-COVID-19 patients with sepsis or septic shock, SARS-CoV-2 infection in 21 patients was associated with significantly higher plasma levels of ten individual LPC species and total LPC concentration. In patients with sepsis/septic shock, LPC species showed negative correlations with procalcitonin and interleukin-6, and positive correlations with gamma-glutamyltransferase and cholesteryl ester levels. In contrast, no significant associations were observed between LPC levels and C-reactive protein, aminotransferases, or free cholesterol. Conclusions: Differential LPC levels, despite comparable disease severity, may serve as metabolic biomarkers to distinguish SARS-CoV-2 sepsis from other causes of sepsis and inform targeted therapeutic approaches. Full article

Influenza vaccines are the primary strategy to prevent severe influenza disease; however, their efficacy is often suboptimal, particularly in older adults (OAs). LiteVax Adjuvant (LVA), a novel adjuvant containing carbohydrate fatty-acid monosulphate ester (CMS) as the active ingredient, has demonstrated a favourable safety profile and enhanced immunogenicity when combined with a low-dose seasonal influenza vaccine in adults aged 18 to 50 years in a first-in-human phase 1 study. The present study investigates the reactogenicity and immunogenicity of CMS-based adjuvanted seasonal influenza vaccine in OAs, with a comparison to responses in younger adults (YAs). In this phase 1b, double-blind, active-controlled clinical trial, 36 YAs (18–50 years) and 48 OAs (≥60 years) were randomized (1:1:1) to receive either 0.5 mg or 1 mg LVA combined with VaxigripTetra, or VaxigripTetra alone. Solicited adverse events (AEs) were recorded using an electronic diary for 7 days following vaccination. Hemagglutination inhibition (HI) titers against four influenza strains were measured at baseline (pre-vaccination) and at 7-, 28-, and 180-days post-vaccination. All 24 YAs and 31 out of 32 OAs receiving CMS-based adjuvanted vaccines reported pain post-vaccination, compared to 8/12 YAs and 4/16 OAs receiving VaxigripTetra. Systemic AEs were more frequently reported among YAs receiving CMS-based adjuvanted vaccines (22/24) compared to those receiving VaxigripTetra (8/12). In OAs, the number of systemic AEs was similar regardless of CMS-based adjuvant administration. Most AEs were mild to moderate and resolved within 3 days. Both CMS-based adjuvanted formulations elicited increased HI titers at Day 7, peaking at Day 28, with a decline thereafter that remained above baseline at Day 180. In YAs, HI titers were comparable between the CMS-based adjuvanted and non-adjuvanted vaccines across all strains and timepoints. In contrast, CMS-based adjuvanted vaccination in OAs induced higher HI titers at Days 28 and 180 for all influenza strains tested. LVA shows an acceptable safety profile in both age cohorts and enhances humoral immune responses in older adults. The 1 mg dose of LVA was more immunogenic, highlighting its potential utility in this target population. Future research will focus on elucidating the mechanisms underlying the immunostimulatory effect of the CMS-based adjuvant. Full article

Previous studies on decision strategies in multi-attribute decision-making (MADM) have primarily relied on computational simulations to assess strategy performance under varying conditions, with particular emphasis on comparisons to the weighted additive rule (WAD) and on evaluations of the cognitive effort required. In contrast, considerably less attention has been devoted to examining the consistency of decision outcomes across different strategies or to developing a systematic classification of strategies based on outcome similarity. To address this gap, the present study investigates the characteristics of decision strategies by analyzing the concordance rates of choices made under identical conditions, along with measures of decision accuracy and information-processing effort. We conducted a hierarchical cluster analysis and applied multi-dimensional scaling (MDS) to a choice concordance matrix derived from simulations using the Mersenne Twister method. In addition, linear multiple regression analyses were performed using the MDS coordinates as predictors of both decision accuracy and cognitive effort. The cluster analysis revealed a primary bifurcation between two major groups: one centered around the Disjunctive (DIS) rule, and another encompassing compensatory strategies such as WAD. Notably, although the Lexicographic (LEX) rule is traditionally considered non-compensatory, it exhibited high similarity in choice patterns to compensatory strategies when assessed via concordance rates. In contrast, DIS-based strategies produced markedly distinct choice patterns. Full article

The discipline of spirituality can be described as the study of human experience of encounter with the transcendent and our lived response to that encounter. There are commonalities to our experience of transcendence that cross the divides of culture and language, commonalities which are often obscured when we theologize about our experience. If we examine the concept of jinn, both among pre-Islamic peoples and in the Qur’an and Hadith and compare this to the demons described in The Life of Antony and The Sayings of the Desert Fathers, we see remarkable similarities. These similarities give evidence that the beliefs in jinn in early Islam and in demons among the Desert Fathers are grounded in a common desert experience. As the centers of theological activity move away from the desert, we find this experience explained by Christianity and Islam in diverse ways. The contrast between descriptive narrative and the subsequent theologizing exemplifies a movement from common spiritual experience to differing theological interpretation. Full article

Background/Objectives: Low semen quality and male infertility are critical global health issues. Emerging research highlights that nutritional factors could play a significant role in determining reproductive outcomes. Understanding and optimizing these dietary influences, including the role of polyphenols, is crucial for developing targeted strategies to improve male fertility. We aimed to explore the relationship between the intake of different classes of polyphenols and semen quality indicators in a cohort of healthy young males. Methods: This is a secondary analysis involving 106 male individuals, aged 18–35 years, from the FERTINUTS trial. Dietary intake was assessed using 3-day dietary records, and semen quality parameters were analyzed. Multivariable linear regression analysis was employed to evaluate the associations between dietary polyphenol consumption and semen quality indicators. Results: Our findings revealed both positive and negative associations between polyphenol consumption and sperm morphology parameters. A higher intake of total polyphenols was associated with a lower percentage of abnormalities in sperm heads but a higher rate of abnormalities in the principal piece. Similar results were observed for lignan and flavonoid intake. Additionally, a higher intake of flavonoids was also associated with a greater percentage of normal sperm forms. In contrast, a higher dietary intake of stilbenes was associated with a higher percentage of combined abnormalities. Conclusions: Higher intake of polyphenols, particularly flavonoids and lignans, was associated with improved sperm head morphology but also with increased tail abnormalities, although no associations with motility or vitality were observed. These results suggest that specific polyphenol classes may have both beneficial and adverse effects on sperm structure, warranting consideration of compound type and dosage in dietary recommendations. Further studies are needed to determine whether these morphological changes impact fertilization outcomes and reproductive potential. Full article