Search Results (11)

Biochar has gained interest as a feed ingredient in livestock nutrition due to its functional properties, circularity, potential to reduce environmental impact, and alignment with sustainable agro-zootechnical practices. The in vivo effects of biochar are closely tied to its physical characteristics, which vary depending on the biomass used as feedstock and the production process. This variability can result in heterogeneity among biochar types used in animal nutrition, leading to inconsistent outcomes. The aim of this study was to characterize the metabolomic and functional properties of an aqueous biochar extract from vine pruning waste, in order to predict its potential in vivo effects as a functional feed ingredient. A metabolomic analysis of the biochar extracts was conducted using quadrupole time-f-light (QQTOF) high-performance liquid chromatography tandem mass spectrometry (HPLC MS/MS). Antimicrobial activity against E. coli F18+ and E. coli F4+ was assessed using standard growth inhibition assays, while quorum sensing in E. coli exposed to biochar extracts was evaluated using real-time PCR. Prebiotic activity was assessed by exposing selected Lactobacillus strains to the biochar extract, monitoring growth patterns to determine species-specific responses. The metabolomic profile revealed several distinct molecular classes, including multiple peaks for phenolic compounds. The extract significantly inhibited the growth of both E. coli pathotypes, reducing growth by 29% and 16% for the F4+ and F18+, respectively (p < 0.001). The relative expression of the genes involved in quorum sensing (MotA, FliA for biofilm formation, and FtsE, HflX for cell division) indicated that the observed inhibitory effects likely resulted from interference with flagellar synthesis, motility, and reduced cell division. The biochar extract also showed species-specific prebiotic potential. In conclusion, biochar derived from vine pruning waste represents a valuable feed ingredient with functional properties that may help to reduce antibiotic use in livestock production. Full article

The objective of this cross-sectional study was to assess eating competence (EC) and the adherence to the division of responsibility in child feeding (sDOR) of Brazilian caregivers of children with celiac disease (CD). It also examined the association between EC and sDOR, children’s adherence to a gluten-free diet, and sociodemographic data. This study administered a survey set that included sociodemographic data, health-related data, eating habits, and the instruments ecSI2.0^TMBR and sDOR.2-6y^TM BR, validated for a Brazilian population. The sample comprised 50 caregivers of children with CD (between 24 and 72 months of age). The participants following a gluten-free diet (GFD) presented higher scores for all EC domains and the total EC. The total EC scores were higher for the participants over 40 y/o, frequently having meals as a family, with their children consuming more than three servings of fruit and at least one serving of vegetables daily and complying with a GFD. Different from the EC, the sDOR.2-6y^TM scores did not differ between the participants complying with a GFD. The sDOR.2-6y^TM mealtime structure domain scores were significantly associated with the EC eating attitude, food acceptance, contextual skills, and total. These findings support the need for greater attention to exploring the division of responsibility in feeding and EC in pediatric celiac disease, potentially enhancing intervention strategies for patients and their families. Full article

Parents’ feeding practices are a function of child eating behaviors, health, and other factors. Adherence to the Satter Division of Responsibility in Feeding (sDOR) model has not been examined relating to child BMI, household food security, or child eating behavior. This study evaluates the adherence to sDOR in relation to child eating behavior, nutrition risk, BMI-for-age, dietary intake, and food security. Ninety-one parent–child (3 to <6 years) dyads completed a cross-sectional asymmetric survey in August–November 2019; n = 69 parents from the original sample completed additional and retrospective questions in June 2021. Main outcomes included sDOR adherence (sDOR.2-6y™), a Child Eating Behavior Questionnaire (CEBQ), nutrition risk (NutriSTEP^®), the USDA 6-item screener, the Block Kids Food Screener, and eating competence (ecSI 2.0™). The children’s weight and height were investigator-measured. Associations were tested with Pearson’s r and Chi Square for continuous and categorical variables, independent sample t-test, one-way ANOVA, or Mann–Whitney U compared means. The dietary comparisons used Spearman’s rho correlation coefficient. sDOR adherence was associated with a lower nutrition risk (r = 0.26, p = 0.03) and showed convergent validation with child eating behavior for three child eating behavior (CEBQ) constructs. sDOR.2-6y™ was not related to the child BMI-for-age z-score (r = 0.11, p = 0.39, n = 69). NutriSTEP^® was associated with dietary quality and higher ecSI 2.0TM (r = 0.32, p = 0.008, n = 69). No associations between sDOR.2-6y™ and food security or dietary intake were noted. Full article

Lactation is a unique reproductive behavior in pigeons, with the crop serving as the organ responsible for secreting pigeon milk. Both male and female pigeons can produce crop milk and rear their offspring through a division of labor. Since the time of the secretion of pigeon crop milk is different in the process of feeding the young, whether the metabolism and formation of pigeon milk use the same mechanism is a very interesting scientific question. However, the metabolic dynamics and underlying genetic mechanisms involved in the formation of pigeon crop milk remain unclear, particularly during the incubation–feeding reproductive cycle. In this study, we integrated lactation-associated metabolism and transcriptome data from the crop tissues of both male and female pigeons during the brooding and feeding stages. We mapped the changes in metabolites related to milk formation in the crop tissues during these stages. Through metabolome profiling, we identified 1413 metabolites among 18 crop tissues. During the breeding cycles, the concentrations of estrone, L-ergothioneine, and L-histidine exhibited the most dynamic changes in females. In contrast, estrone, L-anserine, 1-methylhistidine, homovanillate, oxidized glutathione, and reducing glutathione showed the most dynamic changes in males. Gender-specific differences were observed in the metabolome, with several metabolites significantly differing between males and females, many of which were correlated with cytokine binding, immunity, and cytochrome P450 activity. Using this dataset, we constructed complex regulatory networks, enabling us to identify important metabolites and key genes involved in regulating the formation of pigeon milk in male and female pigeons, respectively. Additionally, we investigated gender-associated differences in the crop metabolites of pigeons. Our study revealed differences in the modulation of pigeon crop milk metabolism between males and females and shed light on the potential functions of male and female pigeon milk in the growth, development, and immunity of young pigeons, an area that has not been previously explored. In conclusion, our results provide new insights into the metabolic regulation of pigeon crop milk formation during the brooding and breeding stages. Furthermore, our findings lay the foundation for the accurate development of artificial pigeon milk. Full article

A 1 × 2 low-profile filtering antenna array is proposed, using an H-shape resonator to feed two strip dense dielectric patch (DDP) resonators. The even–odd mode of the H-shape resonator and the TM_δ1 mode of the strip DDP resonator form the radiation band. Additionally, the odd–odd mode of the H-shape resonator excites the TM_δ2 mode of the strip DDP resonator, thus achieving an upper-edge radiation null for the filtering response. The H-shape resonator not only participates in the antenna array radiation, but also excites two strip media patches at the same time, avoiding the traditional power distribution network and effectively reducing the complexity of the antenna array. In addition, compared with the reported dielectric filtering antenna designs, this design has the advantageous features of a low profile, a compact structure, wide bandwidth and a simplified structure. For example, the antenna prototype operating at 4.9 GHz achieves 10 dB impedance, a matching bandwidth of 7.1%, a maximum gain of 8.6 dBi and the compact size of 1.29 × 0.18 × 0.038 ${\lambda }_0^3$, without requiring a traditional power division network. Full article

This cross-sectional study aimed to translate and perform a psychometric analysis (evaluation of reproducibility and internal consistency) of the sDOR.2-6y™ into Brazilian Portuguese. The translation and back-translation followed the protocol required by the NEEDs Center, and the approved version was called “sDOR.2-6y™—Português-Brasil”. The approved version was submitted to a test–retest round to verify its reproducibility through the Intraclass Correlation Coefficient (ICC). A pilot study was performed to assess the internal consistency of the instrument. The reproducibility analysis (n = 23) showed a total ICC of 0.945. With the data from the pilot study (n = 384), the internal consistency evaluation was analyzed through Cronbach’s alpha coefficient, and the instrument obtained an overall Cronbach’s alpha of 0.301. The translation of the sDOR.2-6y™ into Brazilian Portuguese is the first and only tool available for the Brazilian population to exclusively assess the division of responsibility in feeding, which is essential to the academic community, health professionals, and research on child feeding. Therefore, this instrument in Brazilian Portuguese will allow future research on the division of responsibility in feeding among those responsible for children in Brazil. Full article

This cross-sectional study aimed to assess Brazilian child caregivers’ eating competence (EC) and their adherence to the division of responsibility (sDOR) in child feeding. The research had national coverage in all Brazilian regions. The sample comprised 549 caregivers of Brazilian children (24 up to 72 months) recruited by social media (snowball method). Data on sDOR and EC were collected using the sDOR.2-6y^TM Portuguese—Brazil (sDOR.2-6y-BR) and ecSI2.0^TMBR, both instruments validated to the Brazilian population. The scores of the sDOR.2-6y-BR were described in terms of means, standard deviations (SD), medians, and interquartile range. Student’s t-test and analysis of variance (ANOVA) followed by Tukey’s post hoc tests were used to compare the scores of sDOR.2-6y-BR and ecSI2.0^TMBR with interest variables. The association between the sDOR.2-6y-BR and ecSI2.0^TMBR scores was verified by Pearson’s correlation coefficient. Most of the participants were female (n = 88.7%), 37.8 ± 5.1 y/o, had a high schooling level (70.31%), and high monthly income (more than 15 minimum wages—MW) (31.69%). The children for whom the participants were responsible were mostly girls (53.19%), with an average age of 3.6 ± 1.3 y/o. The instrument presented good responsiveness (floor and ceiling effects = 0%). Cronbach’s Alpha = 0.268. There was no statistical difference in sDOR.2-6y-BR scores between caregiver’s gender, age, level of education, number of people living in the household, or by gender or age of the child. Caregivers who reported (n = 100) that their children had some medical diagnosis (e.g., food allergy, autism, or Down syndrome) had lower sDOR adherence scores than caregivers who indicated their children had no medical diagnosis (p = 0.031). There was no statistical difference in ecSI2.0^TMBR scores between the categories of caregiver’s gender, age, occupancy, and child’s gender and age. Caregivers with income higher than 10 MW, living in houses with more than 3 people, and with graduate schooling showed higher EC scores. Caregivers considered competent eaters by ecSI2.0^TMBR scores differed only for educational level, which was more frequent among graduate participants. The total EC score was positively associated with total and mealtime structure (D1), how food is available to the child (D3), and the parent gives respect to the child’s autonomy in eating (D4) sDOR.2-6y^TM domains. There was a negative association with the what is available to the child (D2) sDOR.2-6y^TM domain. In general, the sDOR.2-6y-BR had a positive association with the ecSI2.0^TMBR in all domains and total, with a low but significant correlation. This study enables the investigation of the division of responsibility in feeding and EC of a sample of caregivers of children in Brazil. This is the first study to apply the translated and validated version of the sDOR.2-6y-BR and showed good results, where competent eaters’ caregivers adhere more to the principles of sDOR. Full article

Hyperosmolality can occur during industrial fed-batch cultivation processes of Chinese hamster ovary (CHO) cells as highly concentrated feed and base solutions are added to replenish nutrients and regulate pH values. Some effects of hyperosmolality, such as increased cell size and growth inhibition, have been elucidated by previous research, but the impact of hyperosmolality and the specific effects of the added osmotic-active reagents have rarely been disentangled. In this study, CHO cells were exposed to four osmotic conditions between 300 mOsm/kg (physiologic condition) and 530 mOsm/kg (extreme hyperosmolality) caused by the addition of either high-glucose-supplemented industrial feed or mannitol as an osmotic control. We present novel single-cell cultivation data revealing heterogeneity in mass gain and cell division in response to these treatments. Exposure to extreme mannitol-induced hyperosmolality and to high-glucose-oversupplemented feed causes cell cycle termination, mtDNA damage, and mitochondrial membrane depolarization, which hints at the onset of premature stress-induced senescence. Thus, this study shows that both mannitol-induced hyperosmolality (530 mOsm/kg) and glucose overfeeding induce severe negative effects on cell growth and mitochondrial activity; therefore, they need to be considered during process development for commercial production. Full article

The aquaculture industry has rapidly increased in response to the increasing world population, with the appreciation that aquaculture products are beneficial for human health and nutrition. Globally, aquaculture organisms are mainly divided into two divisions, aquatic animals (finfish, crustaceans, and molluscs) and aquatic plants (microalgae and seaweed). Worldwide aquaculture production has reached more than 82 million tonnes (MTs) in 2018 with more than 450 cultured species. The development of economical, environmentally friendly, and large-scale feasible technologies to produce aquaculture organisms (even aquatic animals and/or aquatic plants) is an essential need of the world. Some aquaculture technologies are related to aquatic animals or aquatic plants, as well as some technologies have an integrated system. This integration between aquatic plants and aquatic animals could be performed during early larvae rearing, on-growing and/or mass production. In the context of the blue revolution, the current review focuses on the generations of integration between aquatic plants and aquatic animals, such as live feeds, biomass concentrates, water conditioners “green water technique”, aqua-feed additives, co-culturing technologies, and integrated multi-trophic aquaculture (IMTA). This review could shed light on the benefit of aquatic animals and plant integration, which could lead future low-cost, highly efficient, and sustainable aquaculture industry projects. Full article

Large portion sizes can make children overeat, alter their self-regulation abilities and induce weight gain. However, little is known about how parents determine portion sizes for their children. Using semi-structured interviews with 5 fathers and 32 mothers of pre-schoolers, this study examined French parents’ food portioning practices. The division of responsibility between parent and child in deciding portion sizes was explored, as well as the influencing factors and possible sources of information. Parents described a wide range of practices. For most, determining portion sizes is an intuitive action that depends on habits and mainly arises from experiences with feeding their child and his/her appetitive traits. Few parents grant autonomy to their child for portioning and serving food, especially for the first serving. Many influencing factors were identified, including child-related (e.g., appetite, food preferences), parent-related (e.g., avoiding food waste), and external factors (e.g., influence of siblings, French food culture). Most parents do not search for information/recommendations to guide their practices. Stimulating optimal self-regulation of eating in children is important and parents can play a crucial role in this. This study identified barriers and facilitators to guide parents in providing appropriate portion sizes and help include children in this decision process. Full article

This paper proposes a novel receiving antenna allocation scheme for downlink multiuser multiple input multiple output orthogonal frequency division multiplexing (MU-MIMO-OFDM) transmission; an access point (AP) simultaneously transmits data frames to a combination of allocated receiving antennas on a subcarrier basis at each station (STA). The proposed scheme combines a limited channel state information (CSI) feedback sequence with a receiving antenna decision method. In the proposed scheme, each STA estimates the channel responses of all receiving antennas by using the training preamble transmitted from an AP, and then feeds the channel response of the antenna with maximum norm back to the AP when the spatial correlation value between receiving antennas is higher than a threshold. Otherwise, each STA feeds full channel responses back to the AP. This scheme decreases the amount of CSI fed back while exploiting the spatial diversity gain, and the AP’s computational complexity is also decreased regarding the antenna allocation. Moreover, the receiving antenna decision method eliminates the overhead to notify the allocated antenna information from the AP to each STA by simply comparing its own receiving antenna powers. We clarify the effectiveness of the proposed scheme in our computer simulations using channel responses measured in an indoor environment. The results show that the proposed scheme maintains the channel capacity of the downlink MU-MIMO-OFDM transmission while greatly reducing the overhead and computational complexity. Full article